(Linear) (Six-base) MAPSORT of: KIEE4354.seq Check: 3505 from: 1 to: 1113 KIEE4354.orf With 182 enzymes: SgfI * July 3, 2012 19:00 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 117 889 1113 Size: 117 772 224 Fragments arranged by size: 772 224 117 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 977 1113 Size: 977 136 AflIII A'CryG_T Cuts at: 0 409 1113 Size: 409 704 AleI CACnn'nnGTG Cuts at: 0 1081 1113 Size: 1081 32 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 410 1113 Size: 410 703 ApaLI G'TGCA_C Cuts at: 0 210 1113 Size: 210 903 ApoI r'AATT_y Cuts at: 0 691 1113 Size: 691 422 AvaI C'yCGr_G Cuts at: 0 817 1113 Size: 817 296 BamHI G'GATC_C Cuts at: 0 1015 1113 Size: 1015 98 BanI G'GyrC_C Cuts at: 0 248 1113 Size: 248 865 BanII G_rGCy'C Cuts at: 0 463 824 932 1113 Size: 463 361 108 181 Fragments arranged by size: 463 361 181 108 Bme1580I G_kGCm'C Cuts at: 0 214 1113 Size: 214 899 BmgBI CAC'GTC Cuts at: 0 410 1113 Size: 410 703 BmrI ACTGGGnnnn_n' Cuts at: 0 350 1113 Size: 350 763 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 138 211 1006 1113 Size: 138 73 795 107 Fragments arranged by size: 795 138 107 73 BsaAI yAC'GTr Cuts at: 0 372 1104 1113 Size: 372 732 9 Fragments arranged by size: 732 372 9 BsaBI GATnn'nnATC Cuts at: 0 97 1113 Size: 97 1016 BsaWI w'CCGG_w Cuts at: 0 455 1113 Size: 455 658 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 17 47 1113 Size: 17 30 1066 Fragments arranged by size: 1066 30 17 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 86 427 727 940 1009 1113 Size: 86 341 300 213 69 104 Fragments arranged by size: 341 300 213 104 86 69 BseYI C'CCAG_C Cuts at: 0 42 787 1113 Size: 42 745 326 Fragments arranged by size: 745 326 42 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 145 674 923 1113 Size: 145 529 249 190 Fragments arranged by size: 529 249 190 145 BsiHKAI G_wGCw'C Cuts at: 0 214 1113 Size: 214 899 BsmI GAATG_Cn' Cuts at: 0 144 1113 Size: 144 969 BsmBI CGTCTCn'nnnn_ Cuts at: 0 266 1113 Size: 266 847 Bsp1286I G_dGCh'C Cuts at: 0 214 463 824 932 1113 Size: 214 249 361 108 181 Fragments arranged by size: 361 249 214 181 108 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 117 508 889 1113 Size: 117 391 381 224 Fragments arranged by size: 391 381 224 117 BsrBI CCG'CTC Cuts at: 0 1097 1113 Size: 1097 16 BstXI CCAn_nnnn'nTGG Cuts at: 0 605 978 1113 Size: 605 373 135 Fragments arranged by size: 605 373 135 BstYI r'GATC_y Cuts at: 0 432 1015 1113 Size: 432 583 98 Fragments arranged by size: 583 432 98 Bsu36I CC'TnA_GG Cuts at: 0 923 1113 Size: 923 190 BtsI GCAGTG_nn' Cuts at: 0 404 1113 Size: 404 709 DraIII CAC_nnn'GTG Cuts at: 0 476 1113 Size: 476 637 EaeI y'GGCC_r Cuts at: 0 713 947 1113 Size: 713 234 166 Fragments arranged by size: 713 234 166 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 138 211 977 1006 1113 Size: 138 73 766 29 107 Fragments arranged by size: 766 138 107 73 29 EcoNI CCTnn'n_nnAGG Cuts at: 0 226 442 1113 Size: 226 216 671 Fragments arranged by size: 671 226 216 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 17 49 466 498 717 749 790 822 Size: 17 32 417 32 219 32 41 32 Cuts at: 822 1113 Size: 291 Fragments arranged by size: 417 291 219 41 32 32 32 32 17 MscI TGG'CCA Cuts at: 0 715 949 1113 Size: 715 234 164 Fragments arranged by size: 715 234 164 MslI CAynn'nnrTG Cuts at: 0 392 1081 1113 Size: 392 689 32 Fragments arranged by size: 689 392 32 NspI r_CATG'y Cuts at: 0 623 1113 Size: 623 490 PflMI CCAn_nnn'nTGG Cuts at: 0 876 894 1113 Size: 876 18 219 Fragments arranged by size: 876 219 18 PfoI T'CCnGG_A Cuts at: 0 154 227 848 1113 Size: 154 73 621 265 Fragments arranged by size: 621 265 154 73 PmlI CAC'GTG Cuts at: 0 372 1113 Size: 372 741 SfcI C'TryA_G Cuts at: 0 177 1113 Size: 177 936 SmaI CCC'GGG Cuts at: 0 819 1113 Size: 819 294 StuI AGG'CCT Cuts at: 0 199 1006 1113 Size: 199 807 107 Fragments arranged by size: 807 199 107 StyI C'CwwG_G Cuts at: 0 837 1044 1113 Size: 837 207 69 Fragments arranged by size: 837 207 69 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 883 1006 1113 Size: 883 123 107 Fragments arranged by size: 883 123 107 TatI w'GTAC_w Cuts at: 0 87 1113 Size: 87 1026 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 519 723 1113 Size: 519 204 390 Fragments arranged by size: 519 390 204 XmaI C'CCGG_G Cuts at: 0 817 1113 Size: 817 296 Enzymes that do cut: AarI AcuI AflIII AleI AlfI ApaLI ApoI AvaI BamHI BanI BanII Bme1580I BmgBI BmrI BpmI BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BstXI BstYI Bsu36I BtsI DraIII EaeI Eco57MI EcoNI Hin4I MscI MslI NspI PflMI PfoI PmlI SfcI SmaI StuI StyI TaqII TatI XcmI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AloI AlwNI ApaI AscI AseI AsiSI AvrII BaeI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI BmtI BplI Bpu10I BpuEI BsaI BsaHI BsiEI BsiWI BspEI BspHI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgI BtgZI ClaI CspCI DraI DrdI EagI EarI EciI EcoICRI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I XbaI XhoI XmnI ZraI