Restriction map for
pFN21AE9610
(Linear) (Six-base) MAPSORT of: KIEE9610.seq Check: 6811 from: 1 to: 897
KIEE9610.orf
With 182 enzymes: SgfI *
March 10, 2017 10:38 ..
AccI GT'mk_AC
Cuts at: 0 137 897
Size: 137 760
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 664 696 897
Size: 664 32 201
Fragments arranged by size:
664 201 32
AlwNI CAG_nnn'CTG
Cuts at: 0 364 539 897
Size: 364 175 358
Fragments arranged by size:
364 358 175
ApaI G_GGCC'C
Cuts at: 0 118 130 897
Size: 118 12 767
Fragments arranged by size:
767 118 12
AvaI C'yCGr_G
Cuts at: 0 472 554 897
Size: 472 82 343
Fragments arranged by size:
472 343 82
BanII G_rGCy'C
Cuts at: 0 118 130 897
Size: 118 12 767
Fragments arranged by size:
767 118 12
BciVI GTATCCnnnnn_n'
Cuts at: 0 600 897
Size: 600 297
BfrBI ATG'CAT
Cuts at: 0 382 897
Size: 382 515
BglI GCCn_nnn'nGGC
Cuts at: 0 550 897
Size: 550 347
BglII A'GATC_T
Cuts at: 0 174 897
Size: 174 723
Bme1580I G_kGCm'C
Cuts at: 0 62 118 130 897
Size: 62 56 12 767
Fragments arranged by size:
767 62 56 12
BmrI ACTGGGnnnn_n'
Cuts at: 0 374 687 897
Size: 374 313 210
Fragments arranged by size:
374 313 210
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 199 754 897
Size: 37 162 555 143
Fragments arranged by size:
555 162 143 37
Bpu10I CC'TnA_GC
Cuts at: 0 621 897
Size: 621 276
BsaAI yAC'GTr
Cuts at: 0 669 897
Size: 669 228
BsaHI Gr'CG_yC
Cuts at: 0 95 897
Size: 95 802
BsaWI w'CCGG_w
Cuts at: 0 701 897
Size: 701 196
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 325 355 664 694 897
Size: 325 30 309 30 203
Fragments arranged by size:
325 309 203 30 30
BseYI C'CCAG_C
Cuts at: 0 123 269 897
Size: 123 146 628
Fragments arranged by size:
628 146 123
BsiHKAI G_wGCw'C
Cuts at: 0 668 897
Size: 668 229
Bsp1286I G_dGCh'C
Cuts at: 0 62 118 130 668 897
Size: 62 56 12 538 229
Fragments arranged by size:
538 229 62 56 12
BsrBI CCG'CTC
Cuts at: 0 225 897
Size: 225 672
BstYI r'GATC_y
Cuts at: 0 3 174 897
Size: 3 171 723
Fragments arranged by size:
723 171 3
BtgI C'CryG_G
Cuts at: 0 62 131 897
Size: 62 69 766
Fragments arranged by size:
766 69 62
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 760 897
Size: 760 137
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 199 754 897
Size: 37 162 555 143
Fragments arranged by size:
555 162 143 37
EcoO109I rG'GnC_Cy
Cuts at: 0 114 115 127 827 897
Size: 114 1 12 700 70
Fragments arranged by size:
700 114 70 12 1
HaeII r_GCGC'y
Cuts at: 0 187 277 897
Size: 187 90 620
Fragments arranged by size:
620 187 90
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 271 303 604 636 757 789 897
Size: 271 32 301 32 121 32 108
Fragments arranged by size:
301 271 121 108 32 32 32
MspA1I CmG'CkG
Cuts at: 0 38 64 710 897
Size: 38 26 646 187
Fragments arranged by size:
646 187 38 26
NcoI C'CATG_G
Cuts at: 0 131 897
Size: 131 766
NsiI A_TGCA'T
Cuts at: 0 384 897
Size: 384 513
NspI r_CATG'y
Cuts at: 0 315 897
Size: 315 582
PfoI T'CCnGG_A
Cuts at: 0 731 863 897
Size: 731 132 34
Fragments arranged by size:
731 132 34
PmlI CAC'GTG
Cuts at: 0 669 897
Size: 669 228
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 664 696 897
Size: 664 32 201
Fragments arranged by size:
664 201 32
PpuMI rG'GwC_Cy
Cuts at: 0 827 897
Size: 827 70
PspOMI G'GGCC_C
Cuts at: 0 114 126 897
Size: 114 12 771
Fragments arranged by size:
771 114 12
PstI C_TGCA'G
Cuts at: 0 461 599 897
Size: 461 138 298
Fragments arranged by size:
461 298 138
PvuII CAG'CTG
Cuts at: 0 710 897
Size: 710 187
SacII CC_GC'GG
Cuts at: 0 65 897
Size: 65 832
SanDI GG'GwC_CC
Cuts at: 0 827 897
Size: 827 70
SfcI C'TryA_G
Cuts at: 0 457 595 897
Size: 457 138 302
Fragments arranged by size:
457 302 138
SmaI CCC'GGG
Cuts at: 0 474 556 897
Size: 474 82 341
Fragments arranged by size:
474 341 82
SphI G_CATG'C
Cuts at: 0 315 897
Size: 315 582
StyI C'CwwG_G
Cuts at: 0 131 545 897
Size: 131 414 352
Fragments arranged by size:
414 352 131
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 421 897
Size: 421 476
Tth111I GACn'n_nGTC
Cuts at: 0 262 897
Size: 262 635
XmaI C'CCGG_G
Cuts at: 0 472 554 897
Size: 472 82 343
Fragments arranged by size:
472 343 82
Enzymes that do cut:
AccI AloI AlwNI ApaI AvaI BanII BciVI BfrBI
BglI BglII Bme1580I BmrI BpmI Bpu10I BsaAI BsaHI
BsaWI BsaXI BseYI BsiHKAI Bsp1286I BsrBI BstYI BtgI
EciI Eco57MI EcoO109I HaeII Hin4I MspA1I NcoI NsiI
NspI PfoI PmlI PpiI PpuMI PspOMI PstI PvuII
SacII SanDI SfcI SmaI SphI StyI TaqII Tth111I
XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BbsI BbvCI
BcgI BclI BlpI BmgBI BmtI BplI BpuEI BsaI
BsaBI BseRI BsgI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BspMI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI NaeI NarI NdeI NgoMIV NheI NotI NruI
PacI PasI PciI PflMI PmeI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SalI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI
SrfI SspI StuI SwaI TatI TstI XbaI XcmI
XhoI XmnI ZraI