################################## # NO CUT ENZYMES ( 101 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AflIII AgeI AhdI AocI Aor51HI ApaI ApoI AscI AseI AsnI Asp718I AspEI AspI AviII BamHI BbrPI BcgI BciVI BclI BfrI BglI BsaAI BsaWI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI Bst1107I BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DrdI Eam1105I Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KpnI KspI MaeII MfeI MluI MroI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SbfI SexAI SfiI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI Tsp509I Tth111I XbaI XcmI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AccI 298 GTCGAC GT^[AC][GT]AC Asp700I 51 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvrII 757 CCTAGG C^CTAGG BfaI 758 CTAG C^TAG BglII 43 AGATCT A^GATCT BlnI 757 CCTAGG C^CTAGG BlpI 1360 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1360 GCTCAGC GC^T[ACGT]AGC BsaBI 1920 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmBI 892 GAGACG CGTCTC(1/5) BstBI 177 TTCGAA TT^CGAA CelII 1360 GCTCAGC GC^T[ACGT]AGC DraIII 2050 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 1271 CGGCCG C^GGCCG EclXI 1271 CGGCCG C^GGCCG Eco52I 1271 CGGCCG C^GGCCG HincII 298 GTCGAC GT[CT]^[GA]AC HindII 298 GTCGAC GT[CT]^[GA]AC MaeI 758 CTAG C^TAG MamI 1920 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MseI 674 TTAA T^TAA NspV 177 TTCGAA TT^CGAA SalI 298 GTCGAC G^TCGAC ScaI 1277 AGTACT AGT^ACT SfuI 177 TTCGAA TT^CGAA SmaI 1076 CCCGGG CCC^GGG StuI 1892 AGGCCT AGG^CCT TfiI 1007 GATTC G^A[AT]TC Tru9I 674 TTAA T^TAA XmaCI 1076 CCCGGG C^CCGGG XmaI 1076 CCCGGG C^CCGGG XmnI 51 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AcyI 872 GGCGCC G[GA]^CG[CT]C 2084 GGCGCC Alw44I 1333 GTGCAC G^TGCAC 2074 GTGCAC ApaLI 1333 GTGCAC G^TGCAC 2074 GTGCAC BalI 932 TGGCCA TGG^CCA 1655 TGGCCA BbeI 872 GGCGCC GGCGC^C 2084 GGCGCC BbsI 4 GAAGAC GAAGAC(2/6) 994 GAAGAC BbvCI 2109 CCTCAGC CCTCAGC(-5/-2) 1312 GCTGAGG BpuAI 4 GAAGAC GAAGAC(2/6) 994 GAAGAC BsaHI 872 GGCGCC G[GA]^CG[CT]C 2084 GGCGCC BsiEI 300 CGACCG CG[GA][CT]^CG 1271 CGGCCG BsrFI 1909 ACCGGC [GA]^CCGG[CT] 1946 ACCGGC BssHII 797 GCGCGC G^CGCGC 1725 GCGCGC BssSI 1168 CACGAG CACGAG(-5/-1) 1672 CACGAG Cfr10I 1909 ACCGGC [GA]^CCGG[CT] 1946 ACCGGC EarI 2131 CTCTTC CTCTTC(1/4) 841 GAAGAG EcoNI 189 CCTCATCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1668 CCTGCACGAGG Hin1I 872 GGCGCC G[GA]^CG[CT]C 2084 GGCGCC KasI 872 GGCGCC G^GCGCC 2084 GGCGCC Ksp632I 2131 CTCTTC CTCTTC(1/4) 841 GAAGAG MluNI 932 TGGCCA TGG^CCA 1655 TGGCCA MscI 932 TGGCCA TGG^CCA 1655 TGGCCA NarI 872 GGCGCC GG^CGCC 2084 GGCGCC PaeR7I 242 CTCGAG C^TCGAG 2125 CTCGAG PpuMI 1199 GGGTCCT [GA]G^G[AT]CC[CT] 1246 GGGTCCT SapI 841 GAAGAGC GCTCTTC(1/4) 2130 GCTCTTC SfoI 872 GGCGCC GGC^GCC 2084 GGCGCC SphI 143 GCATGC GCATG^C 1301 GCATGC XhoI 242 CTCGAG C^TCGAG 2125 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 97 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI AspHI AvaI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TseI Tsp45I TspRI TthHB8I Van91I XhoII