(Linear) (Six-base) MAPSORT of: KIEE9256.seq Check: 7161 from: 1 to: 2154 KIEE9256.orf With 182 enzymes: SgfI * July 3, 2012 19:02 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 812 1809 2087 2154 Size: 812 997 278 67 Fragments arranged by size: 997 812 278 67 AccI GT'mk_AC Cuts at: 0 299 2154 Size: 299 1855 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 322 505 796 1450 1459 1831 1858 2118 Size: 322 183 291 654 9 372 27 260 Cuts at: 2118 2154 Size: 36 Fragments arranged by size: 654 372 322 291 260 183 36 27 9 AleI CACnn'nnGTG Cuts at: 0 1651 2154 Size: 1651 503 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 448 2154 Size: 448 1706 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1454 1486 2026 2058 2154 Size: 1454 32 540 32 96 Fragments arranged by size: 1454 540 96 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 567 693 1104 1377 1668 2031 2154 Size: 567 126 411 273 291 363 123 Fragments arranged by size: 567 411 363 291 273 126 123 ApaLI G'TGCA_C Cuts at: 0 1333 2074 2154 Size: 1333 741 80 Fragments arranged by size: 1333 741 80 AvaI C'yCGr_G Cuts at: 0 242 1076 2010 2125 2154 Size: 242 834 934 115 29 Fragments arranged by size: 934 834 242 115 29 AvrII C'CTAG_G Cuts at: 0 757 2154 Size: 757 1397 BanI G'GyrC_C Cuts at: 0 872 1744 2084 2154 Size: 872 872 340 70 Fragments arranged by size: 872 872 340 70 BanII G_rGCy'C Cuts at: 0 243 317 1118 1590 1721 2066 2132 2154 Size: 243 74 801 472 131 345 66 22 Fragments arranged by size: 801 472 345 243 131 74 66 22 BbeI G_GCGC'C Cuts at: 0 876 2088 2154 Size: 876 1212 66 Fragments arranged by size: 1212 876 66 BbsI GAAGACnn'nnnn_ Cuts at: 0 11 1001 2154 Size: 11 990 1153 Fragments arranged by size: 1153 990 11 BbvCI CC'TCA_GC Cuts at: 0 1313 2110 2154 Size: 1313 797 44 Fragments arranged by size: 1313 797 44 BglII A'GATC_T Cuts at: 0 43 2154 Size: 43 2111 BlpI GC'TnA_GC Cuts at: 0 1361 2154 Size: 1361 793 Bme1580I G_kGCm'C Cuts at: 0 1337 2078 2154 Size: 1337 741 76 Fragments arranged by size: 1337 741 76 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 99 2154 Size: 99 2055 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 172 274 403 568 616 703 820 934 Size: 172 102 129 165 48 87 117 114 Cuts at: 934 974 1105 1174 1399 1438 1464 1564 1585 Size: 40 131 69 225 39 26 100 21 Cuts at: 1585 1597 2053 2154 Size: 12 456 101 Fragments arranged by size: 456 225 172 165 131 129 117 114 102 101 100 87 69 48 40 39 26 21 12 Bpu10I CC'TnA_GC Cuts at: 0 976 1313 1501 2110 2154 Size: 976 337 188 609 44 Fragments arranged by size: 976 609 337 188 44 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1783 1837 2154 Size: 1783 54 317 Fragments arranged by size: 1783 317 54 BsaI GGTCTCn'nnnn_ Cuts at: 0 463 814 1156 2154 Size: 463 351 342 998 Fragments arranged by size: 998 463 351 342 BsaBI GATnn'nnATC Cuts at: 0 1924 2154 Size: 1924 230 BsaHI Gr'CG_yC Cuts at: 0 873 2085 2154 Size: 873 1212 69 Fragments arranged by size: 1212 873 69 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1733 1763 1927 1957 2026 2056 2154 Size: 1733 30 164 30 69 30 98 Fragments arranged by size: 1733 164 98 69 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 376 805 1126 1159 1396 1501 1531 1561 Size: 376 429 321 33 237 105 30 30 Cuts at: 1561 1754 2154 Size: 193 400 Fragments arranged by size: 429 400 376 321 237 193 105 33 30 30 BseYI C'CCAG_C Cuts at: 0 867 947 1286 1386 1752 2154 Size: 867 80 339 100 366 402 Fragments arranged by size: 867 402 366 339 100 80 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1345 1612 1654 2154 Size: 1345 267 42 500 Fragments arranged by size: 1345 500 267 42 BsiEI CG_ry'CG Cuts at: 0 303 1274 2154 Size: 303 971 880 Fragments arranged by size: 971 880 303 BsiHKAI G_wGCw'C Cuts at: 0 317 1118 1337 1590 1973 2066 2078 2132 Size: 317 801 219 253 383 93 12 54 Cuts at: 2132 2154 Size: 22 Fragments arranged by size: 801 383 317 253 219 93 54 22 12 BsmBI CGTCTCn'nnnn_ Cuts at: 0 886 2154 Size: 886 1268 Bsp1286I G_dGCh'C Cuts at: 0 243 317 1118 1337 1590 1721 1973 2066 Size: 243 74 801 219 253 131 252 93 Cuts at: 2066 2078 2132 2154 Size: 12 54 22 Fragments arranged by size: 801 253 252 243 219 131 93 74 54 22 12 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 593 812 1113 1809 2087 2154 Size: 593 219 301 696 278 67 Fragments arranged by size: 696 593 301 278 219 67 BsrBI CCG'CTC Cuts at: 0 204 345 1221 1533 1581 2154 Size: 204 141 876 312 48 573 Fragments arranged by size: 876 573 312 204 141 48 BsrFI r'CCGG_y Cuts at: 0 1909 1946 2154 Size: 1909 37 208 Fragments arranged by size: 1909 208 37 BssHII G'CGCG_C Cuts at: 0 797 1725 2154 Size: 797 928 429 Fragments arranged by size: 928 797 429 BssSI C'ACGA_G Cuts at: 0 1168 1672 2154 Size: 1168 504 482 Fragments arranged by size: 1168 504 482 BstAPI GCAn_nnn'nTGC Cuts at: 0 144 567 1104 1332 1668 1895 2154 Size: 144 423 537 228 336 227 259 Fragments arranged by size: 537 423 336 259 228 227 144 BstBI TT'CG_AA Cuts at: 0 178 2154 Size: 178 1976 BstXI CCAn_nnnn'nTGG Cuts at: 0 1011 1653 1800 2154 Size: 1011 642 147 354 Fragments arranged by size: 1011 642 354 147 BstYI r'GATC_y Cuts at: 0 43 170 494 678 1874 2154 Size: 43 127 324 184 1196 280 Fragments arranged by size: 1196 324 280 184 127 43 BtgI C'CryG_G Cuts at: 0 23 968 1730 1931 2054 2147 2154 Size: 23 945 762 201 123 93 7 Fragments arranged by size: 945 762 201 123 93 23 7 BtsI GCAGTG_nn' Cuts at: 0 403 514 2154 Size: 403 111 1640 Fragments arranged by size: 1640 403 111 DraIII CAC_nnn'GTG Cuts at: 0 2055 2154 Size: 2055 99 EaeI y'GGCC_r Cuts at: 0 932 1131 1271 1655 2154 Size: 932 199 140 384 499 Fragments arranged by size: 932 499 384 199 140 EagI C'GGCC_G Cuts at: 0 1271 2154 Size: 1271 883 EarI CTCTTCn'nnn_ Cuts at: 0 836 2137 2154 Size: 836 1301 17 Fragments arranged by size: 1301 836 17 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 373 1507 1711 2154 Size: 373 1134 204 443 Fragments arranged by size: 1134 443 373 204 EcoICRI GAG'CTC Cuts at: 0 315 1116 1588 2064 2130 2154 Size: 315 801 472 476 66 24 Fragments arranged by size: 801 476 472 315 66 24 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 172 274 322 403 505 568 616 703 Size: 172 102 48 81 102 63 48 87 Cuts at: 703 796 820 934 974 1105 1174 1399 1438 Size: 93 24 114 40 131 69 225 39 Cuts at: 1438 1450 1459 1464 1564 1585 1597 1831 1858 Size: 12 9 5 100 21 12 234 27 Cuts at: 1858 2053 2118 2154 Size: 195 65 36 Fragments arranged by size: 234 225 195 172 131 114 102 102 100 93 87 81 69 65 63 48 48 40 39 36 27 24 21 12 12 9 5 EcoNI CCTnn'n_nnAGG Cuts at: 0 193 1672 2154 Size: 193 1479 482 Fragments arranged by size: 1479 482 193 EcoO109I rG'GnC_Cy Cuts at: 0 1200 1247 1374 2154 Size: 1200 47 127 780 Fragments arranged by size: 1200 780 127 47 HaeII r_GCGC'y Cuts at: 0 546 876 1061 1076 1177 1349 1668 2088 Size: 546 330 185 15 101 172 319 420 Cuts at: 2088 2154 Size: 66 Fragments arranged by size: 546 420 330 319 185 172 101 66 15 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 663 695 1344 1376 1733 1765 2154 Size: 663 32 649 32 357 32 389 Fragments arranged by size: 663 649 389 357 32 32 32 HincII GTy'rAC Cuts at: 0 300 2154 Size: 300 1854 KasI G'GCGC_C Cuts at: 0 872 2084 2154 Size: 872 1212 70 Fragments arranged by size: 1212 872 70 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 533 1121 2154 Size: 533 588 1033 Fragments arranged by size: 1033 588 533 MscI TGG'CCA Cuts at: 0 934 1657 2154 Size: 934 723 497 Fragments arranged by size: 934 723 497 MslI CAynn'nnrTG Cuts at: 0 196 1651 2071 2154 Size: 196 1455 420 83 Fragments arranged by size: 1455 420 196 83 MspA1I CmG'CkG Cuts at: 0 150 381 579 783 1104 1130 1137 1290 Size: 150 231 198 204 321 26 7 153 Cuts at: 1290 1365 1575 1689 1848 2154 Size: 75 210 114 159 306 Fragments arranged by size: 321 306 231 210 204 198 159 153 150 114 75 26 7 NarI GG'CG_CC Cuts at: 0 873 2085 2154 Size: 873 1212 69 Fragments arranged by size: 1212 873 69 NcoI C'CATG_G Cuts at: 0 23 968 1730 2154 Size: 23 945 762 424 Fragments arranged by size: 945 762 424 23 NspI r_CATG'y Cuts at: 0 147 1305 1416 2154 Size: 147 1158 111 738 Fragments arranged by size: 1158 738 147 111 PflMI CCAn_nnn'nTGG Cuts at: 0 969 1140 2148 2154 Size: 969 171 1008 6 Fragments arranged by size: 1008 969 171 6 PfoI T'CCnGG_A Cuts at: 0 623 836 1877 2154 Size: 623 213 1041 277 Fragments arranged by size: 1041 623 277 213 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2026 2058 2154 Size: 2026 32 96 Fragments arranged by size: 2026 96 32 PpuMI rG'GwC_Cy Cuts at: 0 1200 1247 2154 Size: 1200 47 907 Fragments arranged by size: 1200 907 47 PspXI vC'TCGA_Gb Cuts at: 0 242 2125 2154 Size: 242 1883 29 Fragments arranged by size: 1883 242 29 PstI C_TGCA'G Cuts at: 0 125 509 572 860 1025 1109 1595 1694 Size: 125 384 63 288 165 84 486 99 Cuts at: 1694 2154 Size: 460 Fragments arranged by size: 486 460 384 288 165 125 99 84 63 PvuII CAG'CTG Cuts at: 0 150 381 579 783 1104 1137 1290 1365 Size: 150 231 198 204 321 33 153 75 Cuts at: 1365 1575 1689 2154 Size: 210 114 465 Fragments arranged by size: 465 321 231 210 204 198 153 150 114 75 33 SacI G_AGCT'C Cuts at: 0 317 1118 1590 2066 2132 2154 Size: 317 801 472 476 66 22 Fragments arranged by size: 801 476 472 317 66 22 SalI G'TCGA_C Cuts at: 0 298 2154 Size: 298 1856 SapI GCTCTTCn'nnn_ Cuts at: 0 836 2137 2154 Size: 836 1301 17 Fragments arranged by size: 1301 836 17 ScaI AGT'ACT Cuts at: 0 1279 2154 Size: 1279 875 SfcI C'TryA_G Cuts at: 0 121 505 568 856 1021 1105 1591 1690 Size: 121 384 63 288 165 84 486 99 Cuts at: 1690 2154 Size: 464 Fragments arranged by size: 486 464 384 288 165 121 99 84 63 SfoI GGC'GCC Cuts at: 0 874 2086 2154 Size: 874 1212 68 Fragments arranged by size: 1212 874 68 SmaI CCC'GGG Cuts at: 0 1078 2154 Size: 1078 1076 SmlI C'TyrA_G Cuts at: 0 242 1762 1852 2125 2154 Size: 242 1520 90 273 29 Fragments arranged by size: 1520 273 242 90 29 SphI G_CATG'C Cuts at: 0 147 1305 2154 Size: 147 1158 849 Fragments arranged by size: 1158 849 147 StuI AGG'CCT Cuts at: 0 1894 2154 Size: 1894 260 StyI C'CwwG_G Cuts at: 0 23 757 968 1214 1730 1793 2154 Size: 23 734 211 246 516 63 361 Fragments arranged by size: 734 516 361 246 211 63 23 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1988 2154 Size: 1988 166 TatI w'GTAC_w Cuts at: 0 1277 2154 Size: 1277 877 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 493 525 2154 Size: 493 32 1629 Fragments arranged by size: 1629 493 32 XhoI C'TCGA_G Cuts at: 0 242 2125 2154 Size: 242 1883 29 Fragments arranged by size: 1883 242 29 XmaI C'CCGG_G Cuts at: 0 1076 2154 Size: 1076 1078 XmnI GAAnn'nnTTC Cuts at: 0 55 2154 Size: 55 2099 Enzymes that do cut: AarI AccI AcuI AleI AlfI AloI AlwNI ApaLI AvaI AvrII BanI BanII BbeI BbsI BbvCI BglII BlpI Bme1580I BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI BtgI BtsI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NarI NcoI NspI PflMI PfoI PpiI PpuMI PspXI PstI PvuII SacI SalI SapI ScaI SfcI SfoI SmaI SmlI SphI StuI StyI TaqII TatI TstI XhoI XmaI XmnI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI ApaI ApoI AscI AseI AsiSI BaeI BamHI BcgI BciVI BclI BfrBI BglI BmgBI BmrI BmtI BsaAI BsaWI BsiWI BsmI BspEI BspHI BsrDI BsrGI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KpnI MfeI MluI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PmeI PmlI PshAI PsiI PspOMI PsrI PvuI RsrII SacII SanDI SbfI SexAI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XcmI ZraI