(Linear) (Six-base) MAPSORT of: KIEE3290.seq Check: 6093 from: 1 to: 2241 KIEE3290.orf With 182 enzymes: SgfI * July 3, 2012 17:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 609 2241 Size: 609 1632 AccI GT'mk_AC Cuts at: 0 576 2241 Size: 576 1665 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 181 1828 2241 Size: 181 1647 413 Fragments arranged by size: 1647 413 181 AgeI A'CCGG_T Cuts at: 0 1521 2241 Size: 1521 720 AlwNI CAG_nnn'CTG Cuts at: 0 1942 2019 2169 2196 2241 Size: 1942 77 150 27 45 Fragments arranged by size: 1942 150 77 45 27 ApaI G_GGCC'C Cuts at: 0 1247 2241 Size: 1247 994 ApaLI G'TGCA_C Cuts at: 0 1130 2241 Size: 1130 1111 ApoI r'AATT_y Cuts at: 0 458 700 1201 1366 1481 2218 2241 Size: 458 242 501 165 115 737 23 Fragments arranged by size: 737 501 458 242 165 115 23 AvaI C'yCGr_G Cuts at: 0 884 1180 1625 2241 Size: 884 296 445 616 Fragments arranged by size: 884 616 445 296 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 2143 2176 2241 Size: 2143 33 65 Fragments arranged by size: 2143 65 33 BanI G'GyrC_C Cuts at: 0 1560 1686 2241 Size: 1560 126 555 Fragments arranged by size: 1560 555 126 BanII G_rGCy'C Cuts at: 0 1181 1247 1910 2241 Size: 1181 66 663 331 Fragments arranged by size: 1181 663 331 66 BbsI GAAGACnn'nnnn_ Cuts at: 0 1001 2241 Size: 1001 1240 BbvCI CC'TCA_GC Cuts at: 0 1172 1943 2036 2241 Size: 1172 771 93 205 Fragments arranged by size: 1172 771 205 93 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2219 2241 Size: 2219 22 BfrBI ATG'CAT Cuts at: 0 550 2241 Size: 550 1691 BglII A'GATC_T Cuts at: 0 358 2241 Size: 358 1883 Bme1580I G_kGCm'C Cuts at: 0 977 1134 1247 1691 2241 Size: 977 157 113 444 550 Fragments arranged by size: 977 550 444 157 113 BmrI ACTGGGnnnn_n' Cuts at: 0 145 2241 Size: 145 2096 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 377 2241 Size: 377 1864 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 173 323 664 1186 2041 2191 2241 Size: 173 150 341 522 855 150 50 Fragments arranged by size: 855 522 341 173 150 150 50 Bpu10I CC'TnA_GC Cuts at: 0 1172 1943 2036 2241 Size: 1172 771 93 205 Fragments arranged by size: 1172 771 205 93 BsaI GGTCTCn'nnnn_ Cuts at: 0 1088 2241 Size: 1088 1153 BsaAI yAC'GTr Cuts at: 0 1213 2241 Size: 1213 1028 BsaWI w'CCGG_w Cuts at: 0 1521 2241 Size: 1521 720 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 115 145 2164 2194 2241 Size: 115 30 2019 30 47 Fragments arranged by size: 2019 115 47 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 121 646 1047 1189 2083 2119 2241 Size: 121 525 401 142 894 36 122 Fragments arranged by size: 894 525 401 142 122 121 36 BseYI C'CCAG_C Cuts at: 0 344 2057 2241 Size: 344 1713 184 Fragments arranged by size: 1713 344 184 BsiHKAI G_wGCw'C Cuts at: 0 525 1134 1181 1806 2241 Size: 525 609 47 625 435 Fragments arranged by size: 625 609 525 435 47 BsmI GAATG_Cn' Cuts at: 0 552 1493 2241 Size: 552 941 748 Fragments arranged by size: 941 748 552 Bsp1286I G_dGCh'C Cuts at: 0 525 977 1134 1181 1247 1691 1806 1910 Size: 525 452 157 47 66 444 115 104 Cuts at: 1910 2241 Size: 331 Fragments arranged by size: 525 452 444 331 157 115 104 66 47 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 609 1153 1742 2241 Size: 609 544 589 499 Fragments arranged by size: 609 589 544 499 BsrBI CCG'CTC Cuts at: 0 2010 2241 Size: 2010 231 BsrFI r'CCGG_y Cuts at: 0 1521 2241 Size: 1521 720 BstBI TT'CG_AA Cuts at: 0 1485 2241 Size: 1485 756 BstEII G'GTnAC_C Cuts at: 0 759 2241 Size: 759 1482 BstYI r'GATC_y Cuts at: 0 358 2241 Size: 358 1883 BtsI GCAGTG_nn' Cuts at: 0 525 2235 2241 Size: 525 1710 6 Fragments arranged by size: 1710 525 6 ClaI AT'CG_AT Cuts at: 0 452 2241 Size: 452 1789 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1577 1612 2241 Size: 1577 35 629 Fragments arranged by size: 1577 629 35 EaeI y'GGCC_r Cuts at: 0 2111 2241 Size: 2111 130 EarI CTCTTCn'nnn_ Cuts at: 0 643 1505 1791 1990 2020 2084 2241 Size: 643 862 286 199 30 64 157 Fragments arranged by size: 862 643 286 199 157 64 30 EcoICRI GAG'CTC Cuts at: 0 1179 2241 Size: 1179 1062 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 173 181 323 664 1186 1828 2041 2191 Size: 173 8 142 341 522 642 213 150 Cuts at: 2191 2241 Size: 50 Fragments arranged by size: 642 522 341 213 173 150 142 50 8 EcoO109I rG'GnC_Cy Cuts at: 0 1947 2241 Size: 1947 294 EcoRI G'AATT_C Cuts at: 0 700 1481 2241 Size: 700 781 760 Fragments arranged by size: 781 760 700 EcoRV GAT'ATC Cuts at: 0 1024 2241 Size: 1024 1217 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 283 2241 Size: 283 1958 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 22 54 1318 1350 1521 1553 1918 1950 Size: 22 32 1264 32 171 32 365 32 Cuts at: 1950 2164 2196 2241 Size: 214 32 45 Fragments arranged by size: 1264 365 214 171 45 32 32 32 32 32 22 HincII GTy'rAC Cuts at: 0 231 774 2241 Size: 231 543 1467 Fragments arranged by size: 1467 543 231 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 2045 2241 Size: 2045 196 MscI TGG'CCA Cuts at: 0 2113 2241 Size: 2113 128 MspA1I CmG'CkG Cuts at: 0 1833 1983 2241 Size: 1833 150 258 Fragments arranged by size: 1833 258 150 NdeI CA'TA_TG Cuts at: 0 969 2241 Size: 969 1272 NsiI A_TGCA'T Cuts at: 0 552 2241 Size: 552 1689 PflMI CCAn_nnn'nTGG Cuts at: 0 1555 2064 2169 2241 Size: 1555 509 105 72 Fragments arranged by size: 1555 509 105 72 PfoI T'CCnGG_A Cuts at: 0 1322 2241 Size: 1322 919 PmlI CAC'GTG Cuts at: 0 1213 2241 Size: 1213 1028 PspOMI G'GGCC_C Cuts at: 0 1243 2241 Size: 1243 998 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1628 1660 2241 Size: 1628 32 581 Fragments arranged by size: 1628 581 32 PstI C_TGCA'G Cuts at: 0 1676 2241 Size: 1676 565 PvuII CAG'CTG Cuts at: 0 1833 1983 2241 Size: 1833 150 258 Fragments arranged by size: 1833 258 150 SacI G_AGCT'C Cuts at: 0 1181 2241 Size: 1181 1060 SapI GCTCTTCn'nnn_ Cuts at: 0 2084 2241 Size: 2084 157 ScaI AGT'ACT Cuts at: 0 627 2241 Size: 627 1614 SfcI C'TryA_G Cuts at: 0 1672 2080 2241 Size: 1672 408 161 Fragments arranged by size: 1672 408 161 SmlI C'TyrA_G Cuts at: 0 1180 1625 2241 Size: 1180 445 616 Fragments arranged by size: 1180 616 445 StyI C'CwwG_G Cuts at: 0 62 569 930 2241 Size: 62 507 361 1311 Fragments arranged by size: 1311 507 361 62 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1293 1402 2241 Size: 1293 109 839 Fragments arranged by size: 1293 839 109 TatI w'GTAC_w Cuts at: 0 625 1110 1153 2241 Size: 625 485 43 1088 Fragments arranged by size: 1088 625 485 43 XhoI C'TCGA_G Cuts at: 0 1180 1625 2241 Size: 1180 445 616 Fragments arranged by size: 1180 616 445 XmnI GAAnn'nnTTC Cuts at: 0 1976 2241 Size: 1976 265 Enzymes that do cut: AarI AccI AcuI AgeI AlwNI ApaI ApaLI ApoI AvaI BaeI BanI BanII BbsI BbvCI BcgI BfrBI BglII Bme1580I BmrI BplI BpmI Bpu10I BsaI BsaAI BsaWI BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrFI BstBI BstEII BstYI BtsI ClaI CspCI EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI Hin4I HincII MmeI MscI MspA1I NdeI NsiI PflMI PfoI PmlI PspOMI PsrI PstI PvuII SacI SapI ScaI SfcI SmlI StyI TaqII TatI XhoI XmnI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AflIII AhdI AleI AlfI AloI AscI AseI AsiSI AvrII BamHI BbeI BciVI BclI BglI BlpI BmgBI BmtI BpuEI BsaBI BsaHI BsgI BsiEI BsiWI BsmBI BspEI BspHI BsrDI BsrGI BssHII BssSI BstAPI BstXI BstZ17I Bsu36I BtgI BtgZI DraI DraIII DrdI EagI EciI EcoNI FseI FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI MslI NaeI NarI NcoI NgoMIV NheI NotI NruI NspI PacI PasI PciI PmeI PpiI PpuMI PshAI PsiI PspXI PvuI RsrII SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI Tth111I XbaI XcmI XmaI ZraI