Restriction map for
pFN21AB7634
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# NO CUT ENZYMES ( 131 enzymes) #
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AatII Acc65I AccI AcyI AflII
AflIII AgeI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AvaI AviII AvrII
BalI BbeI BbrPI BbsI BbvCI
BclI BcnI BfaI BfrI BglI
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsiEI BsiWI BsmBI
BsmFI BsoBI BspDI BspHI BspLU11I
BspMI BsrDI BsrFI BssHII Bst1107I
BstEII BstPI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraII DraIII
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
FbaI FseI FspI HaeII HgaI
Hin1I HpaI KasI KpnI KspI
MaeI MamI MfeI MluI MluNI
MscI MunI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
PstI PvuI RcaI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SgrAI SmaI SmlI SnaBI
SpeI Sse8387I SspI StuI SwaI
Tsp45I Van91I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 63 enzymes) #
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AccII 47 CGCG CG^CG
AccIII 228 TCCGGA T^CCGGA
AclI 1061 AACGTT AA^CGTT
AhdI 526 GACTCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 967 GAACTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 526 GACTCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 121 GAGCTC G[AT]GC[AT]^C
AspI 490 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 132 GGACC G^G[AT]CC
BamHI 719 GGATCC G^GATCC
BanI 1051 GGTGCC G^G[CT][GA]CC
BcgI 238 GCATTTAATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 231 GGATAC GTATCC(6/5)
BglII 1070 AGATCT A^GATCT
BpmI 118 CTGGAG CTGGAG(16/14)
BsaWI 228 TCCGGA [AT]^CCGG[AT]
BseAI 228 TCCGGA T^CCGGA
BseRI 106 GAGGAG GAGGAG(10/8)
BsiHKAI 121 GAGCTC G[AT]GC[AT]^C
BsmI 216 GAATGC GAATGC(1/-1)
Bsp1407I 206 TGTACA T^GTACA
BspEI 228 TCCGGA T^CCGGA
BsrGI 206 TGTACA T^GTACA
BssSI 824 CTCGTG CACGAG(-5/-1)
BstAPI 670 GCAAAAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 246 TTCGAA TT^CGAA
BstUI 47 CGCG CG^CG
BstXI 911 CCATCAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr13I 132 GGACC G^G[ACGT]CC
DrdI 334 GACCAGCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 95 CCATGG C^C[GA][CT]GG
EaeI 16 TGGCCG [CT]^GGCC[GA]
Eam1105I 526 GACTCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 790 GAAGAG CTCTTC(1/4)
EcoT14I 95 CCATGG C^C[AT][AT]GG
EcoT22I 218 ATGCAT ATGCA^T
HincII 1044 GTTGAC GT[CT]^[GA]AC
HindII 1044 GTTGAC GT[CT]^[GA]AC
HindIII 838 AAGCTT A^AGCTT
Ksp632I 790 GAAGAG CTCTTC(1/4)
MroI 228 TCCGGA T^CCGGA
MslI 475 CACCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 469 CAGCTG C[AC]G^C[GT]G
MvnI 47 CGCG CG^CG
NcoI 95 CCATGG C^CATGG
NsiI 218 ATGCAT ATGCA^T
NspI 666 GCATGC [GA]CATG^[CT]
NspV 246 TTCGAA TT^CGAA
PflFI 490 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 1061 AACGTT AA^CGTT
PvuII 469 CAGCTG CAG^CTG
SacI 121 GAGCTC GAGCT^C
SapI 790 GAAGAGC GCTCTTC(1/4)
Sau96I 132 GGACC G^G[ACGT]CC
SfcI 604 CTACAG C^T[GA][CT]AG
SfuI 246 TTCGAA TT^CGAA
SphI 666 GCATGC GCATG^C
SspBI 206 TGTACA T^GTACA
StyI 95 CCATGG C^C[AT][AT]GG
TfiI 857 GATTC G^A[AT]TC
Tth111I 490 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 620 CCACTGTAAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 967 GAACTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 16 enzymes) #
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BanII 121 GAGCTC G[GA]GC[CT]^C
727 GAGCCC
BmyI 121 GAGCTC G[AGT]GC[ACT]^C
727 GAGCCC
BsaI 109 GAGACC GGTCTC(1/5)
170 GGTCTC
Bsp1286I 121 GAGCTC G[AGT]GC[ACT]^C
727 GAGCCC
BsrBI 143 CCGCTC CCGCTC(-3/-3)
22 GAGCGG
BsrI 135 CCAGT ACTGG(1/-1)
187 CCAGT
Cac8I 39 GCTCGC GC[ACGT]^[ACGT]GC
666 GCATGC
DraI 868 TTTAAA TTT^AAA
1114 TTTAAA
FauI 63 CCCGC CCCGC(4/6)
24 GCGGG
HapII 19 CCGG C^CGG
229 CCGG
HpaII 19 CCGG C^CGG
229 CCGG
MaeII 422 ACGT A^CGT
1062 ACGT
MaeIII 316 GTTAC ^GT[ACGT]AC
956 GTTAC
MspI 19 CCGG C^CGG
229 CCGG
PleI 490 GACTC GAGTC(4/5)
526 GACTC
SfaNI 220 GCATC GCATC(5/9)
541 GCATC
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# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BbvI BsaJI BsgI BsiYI
BslI BsmAI BssKI BstF5I BstNI
BstYI CfoI DdeI DpnII EcoRII
Fnu4HI FokI HaeIII HhaI HinP1I
HinfI HphI ItaI MboI MboII
MflI MnlI MseI MvaI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
ScrFI TaqI Tru9I TseI Tsp509I
TspRI TthHB8I XhoII