(Circular) (Six-base) MAPSORT of: pFN21AE2116.seq Check: 7932 from: 1 to: 7330 pFN21AE2116 7330 bp With 182 enzymes: SgfI * July 6, 2012 17:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5140 278 Size: 53 83 186 936 3604 2468 Fragments arranged by size: 3604 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2730 2832 3886 3915 4673 1937 Size: 793 102 1054 29 758 4594 Fragments arranged by size: 4594 1054 793 758 102 29 Acc65I G'GTAC_C Cuts at: 4651 4651 Size: 7330 AclI AA'CG_TT Cuts at: 1563 5457 5830 1563 Size: 3894 373 3063 Fragments arranged by size: 3894 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3215 5393 6777 7045 1632 Size: 1583 2178 1384 268 1917 Fragments arranged by size: 2178 1917 1583 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7133 AflIII A'CryG_T Cuts at: 1203 3440 6229 7206 1203 Size: 2237 2789 977 1327 Fragments arranged by size: 2789 2237 1327 977 AgeI A'CCGG_T Cuts at: 6161 6161 Size: 7330 AhdI GACnn_n'nnGTC Cuts at: 6059 6059 Size: 7330 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7330 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7330 AlwNI CAG_nnn'CTG Cuts at: 5073 6645 5073 Size: 1572 5758 ApaLI G'TGCA_C Cuts at: 5387 6543 5387 Size: 1156 6174 ApoI r'AATT_y Cuts at: 1493 2409 2857 3068 3425 3472 3498 3677 3722 Size: 916 448 211 357 47 26 179 45 Cuts at: 3722 4110 4139 4639 4865 5017 7126 1493 Size: 388 29 500 226 152 2109 1697 Fragments arranged by size: 2109 1697 916 500 448 388 357 226 211 179 152 47 45 29 26 AseI AT'TA_AT Cuts at: 160 1032 2238 3354 5884 160 Size: 872 1206 1116 2530 1606 Fragments arranged by size: 2530 1606 1206 1116 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7330 AvaI C'yCGr_G Cuts at: 1870 1943 2222 2838 3273 4655 1870 Size: 73 279 616 435 1382 4545 Fragments arranged by size: 4545 1382 616 435 279 73 BamHI G'GATC_C Cuts at: 4660 5027 4660 Size: 367 6963 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2309 4651 6106 618 Size: 325 217 627 522 2342 1455 1842 Fragments arranged by size: 2342 1842 1455 627 522 325 217 BanII G_rGCy'C Cuts at: 1391 3204 4649 1391 Size: 1813 1445 4072 Fragments arranged by size: 4072 1813 1445 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2568 2625 928 Size: 971 669 57 5633 Fragments arranged by size: 5633 971 669 57 BbvCI CC'TCA_GC Cuts at: 2484 2484 Size: 7330 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5521 5555 1903 Size: 34 3584 34 3678 Fragments arranged by size: 3678 3584 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5222 6432 5222 Size: 1210 6120 BclI T'GATC_A Cuts at: 1548 2976 4035 1548 Size: 1428 1059 4843 Fragments arranged by size: 4843 1428 1059 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5941 136 Size: 107 122 71 631 605 4269 1525 Fragments arranged by size: 4269 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4060 7325 4060 Size: 3265 4065 BlpI GC'TnA_GC Cuts at: 2284 4747 2284 Size: 2463 4867 Bme1580I G_kGCm'C Cuts at: 1790 2314 4439 5391 6547 1790 Size: 524 2125 952 1156 2573 Fragments arranged by size: 2573 2125 1156 952 524 BmgBI CAC'GTC Cuts at: 1318 3590 1318 Size: 2272 5058 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3869 3965 5061 Size: 416 53 83 399 24 2452 96 1096 Cuts at: 5061 6019 442 Size: 958 1753 Fragments arranged by size: 2452 1753 1096 958 416 399 96 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2070 1056 Size: 1014 6316 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5990 1239 Size: 4751 2579 Bpu10I CC'TnA_GC Cuts at: 2484 2484 Size: 7330 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2315 2623 4546 5455 6320 6618 2315 Size: 308 1923 909 865 298 3027 Fragments arranged by size: 3027 1923 909 865 308 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5993 882 Size: 617 4494 2219 Fragments arranged by size: 4494 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4605 5121 493 Size: 711 3401 516 2702 Fragments arranged by size: 3401 2702 711 516 BsaBI GATnn'nnATC Cuts at: 2259 7324 2259 Size: 5065 2265 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5137 5519 6154 275 Size: 53 83 186 936 3604 382 635 1451 Fragments arranged by size: 3604 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2505 4552 5763 6161 6435 6582 6993 2505 Size: 2047 1211 398 274 147 411 2842 Fragments arranged by size: 2842 2047 1211 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3150 3180 1178 Size: 30 165 30 1747 30 5328 Fragments arranged by size: 5328 1747 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 7330 BseYI C'CCAG_C Cuts at: 6533 7029 6533 Size: 496 6834 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2000 2134 1163 Size: 573 264 134 6359 Fragments arranged by size: 6359 573 264 134 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4780 5541 6569 1540 Size: 275 186 2779 761 1028 2301 Fragments arranged by size: 2779 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4450 5391 5476 6547 4450 Size: 941 85 1071 5233 Fragments arranged by size: 5233 1071 941 85 BsmI GAATG_Cn' Cuts at: 3463 4841 4934 3463 Size: 1378 93 5859 Fragments arranged by size: 5859 1378 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2314 3204 4439 4450 4649 5391 5476 Size: 399 524 890 1235 11 199 742 85 Cuts at: 5476 6547 1391 Size: 1071 2174 Fragments arranged by size: 2174 1235 1071 890 742 524 399 199 85 11 BspEI T'CCGG_A Cuts at: 2505 4552 6993 2505 Size: 2047 2441 2842 Fragments arranged by size: 2842 2441 2047 BspHI T'CATG_A Cuts at: 5219 6949 5219 Size: 1730 5600 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4685 844 Size: 3841 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5217 1394 Size: 429 3394 3507 Fragments arranged by size: 3507 3394 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3070 5825 5999 65 Size: 1183 1822 2755 174 1396 Fragments arranged by size: 2755 1822 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5974 6161 1700 Size: 110 4164 187 2869 Fragments arranged by size: 4164 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3507 3895 96 Size: 3411 388 3531 Fragments arranged by size: 3531 3411 388 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7330 BssSI C'ACGA_G Cuts at: 5390 6402 5390 Size: 1012 6318 BstAPI GCAn_nnn'nTGC Cuts at: 794 2600 794 Size: 1806 5524 BstBI TT'CG_AA Cuts at: 3119 6134 3119 Size: 3015 4315 BstEII G'GTnAC_C Cuts at: 1184 4070 4222 4512 1184 Size: 2886 152 290 4002 Fragments arranged by size: 4002 2886 290 152 BstYI r'GATC_y Cuts at: 1972 2842 4060 4660 5027 5412 5429 6881 6967 Size: 870 1218 600 367 385 17 1452 86 Cuts at: 6967 6979 7325 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1218 870 600 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2833 3887 2833 Size: 1054 6276 BtgI C'CryG_G Cuts at: 513 1065 2004 3906 513 Size: 552 939 1902 3937 Fragments arranged by size: 3937 1902 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2954 487 Size: 683 56 1728 4863 Fragments arranged by size: 4863 1728 683 56 BtsI GCAGTG_nn' Cuts at: 4846 5640 5660 4846 Size: 794 20 6516 Fragments arranged by size: 6516 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7330 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3135 3170 529 Size: 35 2571 35 4689 Fragments arranged by size: 4689 2571 35 35 DraI TTT'AAA Cuts at: 2371 3990 4634 2371 Size: 1619 644 5067 Fragments arranged by size: 5067 1619 644 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6337 809 Size: 507 5021 1802 Fragments arranged by size: 5021 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4777 5666 7295 Size: 54 1425 50 275 18 2947 889 1629 Cuts at: 7295 8 Size: 43 Fragments arranged by size: 2947 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4777 1537 Size: 275 2965 4090 Fragments arranged by size: 4090 2965 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2388 2461 2550 3192 3339 4421 5260 1353 Size: 1035 73 89 642 147 1082 839 3423 Fragments arranged by size: 3423 1082 1035 839 642 147 89 73 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5917 6291 6437 387 Size: 1064 138 4328 374 146 1280 Fragments arranged by size: 4328 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3215 5393 5990 6777 7045 1239 Size: 393 1583 2178 597 787 268 1524 Fragments arranged by size: 2178 1583 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 4455 4455 Size: 7330 EcoO109I rG'GnC_Cy Cuts at: 2687 3269 2687 Size: 582 6748 EcoRI G'AATT_C Cuts at: 3498 4639 5017 3498 Size: 1141 378 5811 Fragments arranged by size: 5811 1141 378 EcoRV GAT'ATC Cuts at: 4050 4050 Size: 7330 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2304 4592 2304 Size: 2288 5042 FspI TGC'GCA Cuts at: 5836 5836 Size: 7330 HaeII r_GCGC'y Cuts at: 1678 2358 6477 1678 Size: 680 4119 2531 Fragments arranged by size: 4119 2531 680 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2684 2716 4072 4104 5971 Size: 32 135 32 1112 32 1356 32 1867 Cuts at: 5971 6003 6045 6077 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1867 1356 1112 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2124 4674 4920 669 Size: 988 281 186 2550 246 3079 Fragments arranged by size: 3079 2550 988 281 246 186 HindIII A'AGCT_T Cuts at: 748 748 Size: 7330 HpaI GTT'AAC Cuts at: 2124 4920 2124 Size: 2796 4534 KpnI G_GTAC'C Cuts at: 4655 4655 Size: 7330 MfeI C'AATT_G Cuts at: 4929 4929 Size: 7330 MluI A'CGCG_T Cuts at: 7206 7206 Size: 7330 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2201 6444 6628 1305 Size: 896 4243 184 2007 Fragments arranged by size: 4243 2007 896 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5508 Fragments arranged by size: 5508 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 5288 5647 5806 518 Size: 648 4122 359 159 2042 Fragments arranged by size: 4122 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2087 3733 4746 5423 6571 6816 1606 Size: 481 1646 1013 677 1148 245 2120 Fragments arranged by size: 2120 1646 1148 1013 677 481 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3906 513 Size: 552 939 1902 3937 Fragments arranged by size: 3937 1902 939 552 NdeI CA'TA_TG Cuts at: 387 4218 387 Size: 3831 3499 NheI G'CTAG_C Cuts at: 1052 2066 1052 Size: 1014 6316 NotI GC'GGCC_GC Cuts at: 4777 4777 Size: 7330 NspI r_CATG'y Cuts at: 1132 1229 2598 3444 3508 4627 4688 6233 1132 Size: 97 1369 846 64 1119 61 1545 2229 Fragments arranged by size: 2229 1545 1369 1119 846 97 64 61 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7330 PciI A'CATG_T Cuts at: 3440 6229 3440 Size: 2789 4541 PflMI CCAn_nnn'nTGG Cuts at: 1108 3764 1108 Size: 2656 4674 PmeI GTTT'AAAC Cuts at: 4634 4634 Size: 7330 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3413 3445 5399 5431 6912 6944 3413 Size: 32 1954 32 1481 32 3799 Fragments arranged by size: 3799 1954 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3269 3269 Size: 7330 PshAI GACnn'nnGTC Cuts at: 1613 2986 1613 Size: 1373 5957 PsiI TTA'TAA Cuts at: 2232 3051 4900 2232 Size: 819 1849 4662 Fragments arranged by size: 4662 1849 819 PspXI vC'TCGA_Gb Cuts at: 2222 3273 2222 Size: 1051 6279 PstI C_TGCA'G Cuts at: 830 3652 4682 830 Size: 2822 1030 3478 Fragments arranged by size: 3478 2822 1030 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7330 PvuII CAG'CTG Cuts at: 2087 3733 2087 Size: 1646 5684 SalI G'TCGA_C Cuts at: 1936 4672 1936 Size: 2736 4594 SapI GCTCTTCn'nnn_ Cuts at: 3192 3192 Size: 7330 SbfI CC_TGCA'GG Cuts at: 4682 4682 Size: 7330 ScaI AGT'ACT Cuts at: 3858 4247 5578 7290 3858 Size: 389 1331 1712 3898 Fragments arranged by size: 3898 1712 1331 389 SfcI C'TryA_G Cuts at: 826 1045 3535 3648 3916 4323 4678 5813 6494 Size: 219 2490 113 268 407 355 1135 681 Cuts at: 6494 6685 826 Size: 191 1471 Fragments arranged by size: 2490 1471 1135 681 407 355 268 219 191 113 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7330 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7330 SmaI CCC'GGG Cuts at: 1872 4657 1872 Size: 2785 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2222 2294 2638 2838 3273 4561 Size: 197 926 279 72 344 200 435 1288 Cuts at: 4561 5434 6335 6597 820 Size: 873 901 262 1553 Fragments arranged by size: 1553 1288 926 901 873 435 344 279 262 200 197 72 SnaBI TAC'GTA Cuts at: 493 5121 493 Size: 4628 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7330 SphI G_CATG'C Cuts at: 1132 4627 4688 1132 Size: 3495 61 3774 Fragments arranged by size: 3774 3495 61 SspI AAT'ATT Cuts at: 5 52 5254 5 Size: 47 5202 2081 Fragments arranged by size: 5202 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7330 StyI C'CwwG_G Cuts at: 513 1065 2004 2526 2965 3906 4769 513 Size: 552 939 522 439 941 863 3074 Fragments arranged by size: 3074 941 939 863 552 522 439 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3984 5357 5374 5527 5712 6157 325 Size: 1255 2404 1373 17 153 185 445 1498 Fragments arranged by size: 2404 1498 1373 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3507 3856 3895 Size: 274 80 33 51 1443 1530 349 39 Cuts at: 3895 4245 5576 7288 96 Size: 350 1331 1712 138 Fragments arranged by size: 1712 1530 1443 1331 350 349 274 138 80 51 39 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3150 3182 7290 7322 3150 Size: 32 4108 32 3158 Fragments arranged by size: 4108 3158 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7330 XbaI T'CTAG_A Cuts at: 3376 4297 4666 3376 Size: 921 369 6040 Fragments arranged by size: 6040 921 369 XhoI C'TCGA_G Cuts at: 1943 2222 2838 3273 1943 Size: 279 616 435 6000 Fragments arranged by size: 6000 616 435 279 XmaI C'CCGG_G Cuts at: 1870 4655 1870 Size: 2785 4545 XmnI GAAnn'nnTTC Cuts at: 2861 3425 3726 4383 5459 2861 Size: 564 301 657 1076 4732 Fragments arranged by size: 4732 1076 657 564 301 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5138 276 Size: 53 83 186 936 3604 2468 Fragments arranged by size: 3604 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI