Restriction map for pFN21AE2116
 (Linear) (Six-base) MAPSORT of: KIEE2116.seq  Check: 9235  from: 1  to: 2625



KIEE2116.orf

 With 182 enzymes: SgfI * 

                        July 3, 2012 17:09  ..

AccI GT'mk_AC

Cuts at:      0     725     827    1881    1910    2625 
   Size:        725     102    1054      29     715

  Fragments arranged by size: 

                1054     725     715     102      29
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1210    2625 
   Size:       1210    1415
 
AflIII A'CryG_T

Cuts at:      0    1435    2625 
   Size:       1435    1190
 
ApoI r'AATT_y

Cuts at:      0     404     852    1063    1420    1467    1493    1672    1717 
   Size:        404     448     211     357      47      26     179      45

Cuts at:   1717    2105    2134    2625 
   Size:        388      29     491

  Fragments arranged by size: 

                 491     448     404     388     357     211     179      47
                  45      29      26
 
AseI AT'TA_AT

Cuts at:      0     233    1349    2625 
   Size:        233    1116    1276

  Fragments arranged by size: 

                1276    1116     233
 
AvaI C'yCGr_G

Cuts at:      0     217     833    1268    2625 
   Size:        217     616     435    1357

  Fragments arranged by size: 

                1357     616     435     217
 
BanI G'GyrC_C

Cuts at:      0     304    2625 
   Size:        304    2321
 
BanII G_rGCy'C

Cuts at:      0    1199    2625 
   Size:       1199    1426
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     563     620    2625 
   Size:        563      57    2005

  Fragments arranged by size: 

                2005     563      57
 
BbvCI CC'TCA_GC

Cuts at:      0     479    2625 
   Size:        479    2146
 
BclI T'GATC_A

Cuts at:      0     971    2030    2625 
   Size:        971    1059     595

  Fragments arranged by size: 

                1059     971     595
 
BglII A'GATC_T

Cuts at:      0    2055    2625 
   Size:       2055     570
 
BlpI GC'TnA_GC

Cuts at:      0     279    2625 
   Size:        279    2346
 
Bme1580I G_kGCm'C

Cuts at:      0     309    2434    2625 
   Size:        309    2125     191

  Fragments arranged by size: 

                2125     309     191
 
BmgBI CAC'GTC

Cuts at:      0    1585    2625 
   Size:       1585    1040
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1864    1960    2625 
   Size:       1864      96     665

  Fragments arranged by size: 

                1864     665      96
 
BmtI G_CTAG'C

Cuts at:      0      65    2625 
   Size:         65    2560
 
Bpu10I CC'TnA_GC

Cuts at:      0     479    2625 
   Size:        479    2146
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     310     618    2541    2625 
   Size:        310     308    1923      84

  Fragments arranged by size: 

                1923     310     308      84
 
BsaAI yAC'GTr

Cuts at:      0    2600    2625 
   Size:       2600      25
 
BsaBI GATnn'nnATC

Cuts at:      0     254    2625 
   Size:        254    2371
 
BsaWI w'CCGG_w

Cuts at:      0     500    2547    2625 
   Size:        500    2047      78

  Fragments arranged by size: 

                2047     500      78
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1145    1175    2625 
   Size:       1145      30    1450

  Fragments arranged by size: 

                1450    1145      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     129    2625 
   Size:        129    2496
 
BsiHKAI G_wGCw'C

Cuts at:      0    2445    2625 
   Size:       2445     180
 
BsmI GAATG_Cn'

Cuts at:      0    1458    2625 
   Size:       1458    1167
 
Bsp1286I G_dGCh'C

Cuts at:      0     309    1199    2434    2445    2625 
   Size:        309     890    1235      11     180

  Fragments arranged by size: 

                1235     890     309     180      11
 
BspEI T'CCGG_A

Cuts at:      0     500    2547    2625 
   Size:        500    2047      78

  Fragments arranged by size: 

                2047     500      78
 
BsrDI GCAATG_nn'

Cuts at:      0    1065    2625 
   Size:       1065    1560
 
BsrGI T'GTAC_A

Cuts at:      0    1502    1890    2625 
   Size:       1502     388     735

  Fragments arranged by size: 

                1502     735     388
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     595    2625 
   Size:        595    2030
 
BstBI TT'CG_AA

Cuts at:      0    1114    2625 
   Size:       1114    1511
 
BstEII G'GTnAC_C

Cuts at:      0    2065    2217    2507    2625 
   Size:       2065     152     290     118

  Fragments arranged by size: 

                2065     290     152     118
 
BstYI r'GATC_y

Cuts at:      0     837    2055    2625 
   Size:        837    1218     570

  Fragments arranged by size: 

                1218     837     570
 
BstZ17I GTA'TAC

Cuts at:      0     828    1882    2625 
   Size:        828    1054     743

  Fragments arranged by size: 

                1054     828     743
 
BtgI C'CryG_G

Cuts at:      0    1901    2625 
   Size:       1901     724
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     949    2625 
   Size:        949    1676
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1130    1165    2625 
   Size:       1130      35    1460

  Fragments arranged by size: 

                1460    1130      35
 
DraI TTT'AAA

Cuts at:      0     366    1985    2625 
   Size:        366    1619     640

  Fragments arranged by size: 

                1619     640     366
 
EarI CTCTTCn'nnn_

Cuts at:      0     383     456     545    1187    1334    2416    2625 
   Size:        383      73      89     642     147    1082     209

  Fragments arranged by size: 

                1082     642     383     209     147      89      73
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1210    2625 
   Size:       1210    1415
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2450    2625 
   Size:       2450     175
 
EcoO109I rG'GnC_Cy

Cuts at:      0     682    1264    2625 
   Size:        682     582    1361

  Fragments arranged by size: 

                1361     682     582
 
EcoRI G'AATT_C

Cuts at:      0    1493    2625 
   Size:       1493    1132
 
EcoRV GAT'ATC

Cuts at:      0    2045    2625 
   Size:       2045     580
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     299    2587    2625 
   Size:        299    2288      38

  Fragments arranged by size: 

                2288     299      38
 
HaeII r_GCGC'y

Cuts at:      0     353    2625 
   Size:        353    2272
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     679     711    2067    2099    2625 
   Size:        679      32    1356      32     526

  Fragments arranged by size: 

                1356     679     526      32      32
 
HincII GTy'rAC

Cuts at:      0     119    2625 
   Size:        119    2506
 
HpaI GTT'AAC

Cuts at:      0     119    2625 
   Size:        119    2506
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     196    2625 
   Size:        196    2429
 
MspA1I CmG'CkG

Cuts at:      0      82    1728    2625 
   Size:         82    1646     897

  Fragments arranged by size: 

                1646     897      82
 
NcoI C'CATG_G

Cuts at:      0    1901    2625 
   Size:       1901     724
 
NdeI CA'TA_TG

Cuts at:      0    2213    2625 
   Size:       2213     412
 
NheI G'CTAG_C

Cuts at:      0      61    2625 
   Size:         61    2564
 
NspI r_CATG'y

Cuts at:      0     593    1439    1503    2622    2625 
   Size:        593     846      64    1119       3

  Fragments arranged by size: 

                1119     846     593      64       3
 
PciI A'CATG_T

Cuts at:      0    1435    2625 
   Size:       1435    1190
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1759    2625 
   Size:       1759     866
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1408    1440    2625 
   Size:       1408      32    1185

  Fragments arranged by size: 

                1408    1185      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1264    2625 
   Size:       1264    1361
 
PshAI GACnn'nnGTC

Cuts at:      0     981    2625 
   Size:        981    1644
 
PsiI TTA'TAA

Cuts at:      0     227    1046    2625 
   Size:        227     819    1579

  Fragments arranged by size: 

                1579     819     227
 
PspXI vC'TCGA_Gb

Cuts at:      0     217    1268    2625 
   Size:        217    1051    1357

  Fragments arranged by size: 

                1357    1051     217
 
PstI C_TGCA'G

Cuts at:      0    1647    2625 
   Size:       1647     978
 
PvuII CAG'CTG

Cuts at:      0      82    1728    2625 
   Size:         82    1646     897

  Fragments arranged by size: 

                1646     897      82
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1187    2625 
   Size:       1187    1438
 
ScaI AGT'ACT

Cuts at:      0    1853    2242    2625 
   Size:       1853     389     383

  Fragments arranged by size: 

                1853     389     383
 
SfcI C'TryA_G

Cuts at:      0    1530    1643    1911    2318    2625 
   Size:       1530     113     268     407     307

  Fragments arranged by size: 

                1530     407     307     268     113
 
SmlI C'TyrA_G

Cuts at:      0     217     289     633     833    1268    2556    2625 
   Size:        217      72     344     200     435    1288      69

  Fragments arranged by size: 

                1288     435     344     217     200      72      69
 
SphI G_CATG'C

Cuts at:      0    2622    2625 
   Size:       2622       3
 
StyI C'CwwG_G

Cuts at:      0     521     960    1901    2625 
   Size:        521     439     941     724

  Fragments arranged by size: 

                 941     724     521     439
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1979    2625 
   Size:       1979     646
 
TatI w'GTAC_w

Cuts at:      0    1502    1851    1890    2240    2625 
   Size:       1502     349      39     350     385

  Fragments arranged by size: 

                1502     385     350     349      39
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1145    1177    2625 
   Size:       1145      32    1448

  Fragments arranged by size: 

                1448    1145      32
 
XbaI T'CTAG_A

Cuts at:      0    1371    2292    2625 
   Size:       1371     921     333

  Fragments arranged by size: 

                1371     921     333
 
XhoI C'TCGA_G

Cuts at:      0     217     833    1268    2625 
   Size:        217     616     435    1357

  Fragments arranged by size: 

                1357     616     435     217
 
XmnI GAAnn'nnTTC

Cuts at:      0     856    1420    1721    2378    2625 
   Size:        856     564     301     657     247

  Fragments arranged by size: 

                 856     657     564     301     247
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     ApoI     AseI     AvaI     BanI    BanII
     BbsI    BbvCI     BclI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaWI    BsaXI     BsgI
  BsiHKAI     BsmI Bsp1286I    BspEI    BsrDI    BsrGI   BstAPI    BstBI
   BstEII    BstYI  BstZ17I     BtgI    BtgZI    CspCI     DraI     EarI
  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI    HaeII    Hin4I
   HincII     HpaI     MmeI   MspA1I     NcoI     NdeI     NheI     NspI
     PciI    PflMI     PpiI    PpuMI    PshAI     PsiI    PspXI     PstI
    PvuII     SapI     ScaI     SfcI     SmlI     SphI     StyI    TaqII
     TatI     TstI     XbaI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI    AsiSI
    AvrII     BaeI    BamHI     BbeI     BcgI    BciVI    BfrBI     BglI
     BplI     BpmI     BsaI    BsaHI    BseRI    BseYI    BsiEI    BsiWI
    BsmBI    BspHI    BspMI    BsrBI    BsrFI   BssHII    BssSI    BstXI
   Bsu36I     BtsI     ClaI   DraIII     DrdI     EaeI     EagI     EciI
  EcoICRI     FseI     FspI    FspAI  HindIII     KasI     KpnI     MfeI
     MluI     MscI     MslI     NaeI     NarI   NgoMIV     NotI     NruI
     NsiI     PacI     PasI     PfoI     PmeI     PmlI   PspOMI     PsrI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SbfI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI
     SspI     StuI     SwaI  Tth111I     XcmI     XmaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com