(Linear) (Six-base) MAPSORT of: KIEE2116.seq Check: 9235 from: 1 to: 2625 KIEE2116.orf With 182 enzymes: SgfI * July 3, 2012 17:09 .. AccI GT'mk_AC Cuts at: 0 725 827 1881 1910 2625 Size: 725 102 1054 29 715 Fragments arranged by size: 1054 725 715 102 29 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1210 2625 Size: 1210 1415 AflIII A'CryG_T Cuts at: 0 1435 2625 Size: 1435 1190 ApoI r'AATT_y Cuts at: 0 404 852 1063 1420 1467 1493 1672 1717 Size: 404 448 211 357 47 26 179 45 Cuts at: 1717 2105 2134 2625 Size: 388 29 491 Fragments arranged by size: 491 448 404 388 357 211 179 47 45 29 26 AseI AT'TA_AT Cuts at: 0 233 1349 2625 Size: 233 1116 1276 Fragments arranged by size: 1276 1116 233 AvaI C'yCGr_G Cuts at: 0 217 833 1268 2625 Size: 217 616 435 1357 Fragments arranged by size: 1357 616 435 217 BanI G'GyrC_C Cuts at: 0 304 2625 Size: 304 2321 BanII G_rGCy'C Cuts at: 0 1199 2625 Size: 1199 1426 BbsI GAAGACnn'nnnn_ Cuts at: 0 563 620 2625 Size: 563 57 2005 Fragments arranged by size: 2005 563 57 BbvCI CC'TCA_GC Cuts at: 0 479 2625 Size: 479 2146 BclI T'GATC_A Cuts at: 0 971 2030 2625 Size: 971 1059 595 Fragments arranged by size: 1059 971 595 BglII A'GATC_T Cuts at: 0 2055 2625 Size: 2055 570 BlpI GC'TnA_GC Cuts at: 0 279 2625 Size: 279 2346 Bme1580I G_kGCm'C Cuts at: 0 309 2434 2625 Size: 309 2125 191 Fragments arranged by size: 2125 309 191 BmgBI CAC'GTC Cuts at: 0 1585 2625 Size: 1585 1040 BmrI ACTGGGnnnn_n' Cuts at: 0 1864 1960 2625 Size: 1864 96 665 Fragments arranged by size: 1864 665 96 BmtI G_CTAG'C Cuts at: 0 65 2625 Size: 65 2560 Bpu10I CC'TnA_GC Cuts at: 0 479 2625 Size: 479 2146 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 310 618 2541 2625 Size: 310 308 1923 84 Fragments arranged by size: 1923 310 308 84 BsaAI yAC'GTr Cuts at: 0 2600 2625 Size: 2600 25 BsaBI GATnn'nnATC Cuts at: 0 254 2625 Size: 254 2371 BsaWI w'CCGG_w Cuts at: 0 500 2547 2625 Size: 500 2047 78 Fragments arranged by size: 2047 500 78 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1145 1175 2625 Size: 1145 30 1450 Fragments arranged by size: 1450 1145 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 129 2625 Size: 129 2496 BsiHKAI G_wGCw'C Cuts at: 0 2445 2625 Size: 2445 180 BsmI GAATG_Cn' Cuts at: 0 1458 2625 Size: 1458 1167 Bsp1286I G_dGCh'C Cuts at: 0 309 1199 2434 2445 2625 Size: 309 890 1235 11 180 Fragments arranged by size: 1235 890 309 180 11 BspEI T'CCGG_A Cuts at: 0 500 2547 2625 Size: 500 2047 78 Fragments arranged by size: 2047 500 78 BsrDI GCAATG_nn' Cuts at: 0 1065 2625 Size: 1065 1560 BsrGI T'GTAC_A Cuts at: 0 1502 1890 2625 Size: 1502 388 735 Fragments arranged by size: 1502 735 388 BstAPI GCAn_nnn'nTGC Cuts at: 0 595 2625 Size: 595 2030 BstBI TT'CG_AA Cuts at: 0 1114 2625 Size: 1114 1511 BstEII G'GTnAC_C Cuts at: 0 2065 2217 2507 2625 Size: 2065 152 290 118 Fragments arranged by size: 2065 290 152 118 BstYI r'GATC_y Cuts at: 0 837 2055 2625 Size: 837 1218 570 Fragments arranged by size: 1218 837 570 BstZ17I GTA'TAC Cuts at: 0 828 1882 2625 Size: 828 1054 743 Fragments arranged by size: 1054 828 743 BtgI C'CryG_G Cuts at: 0 1901 2625 Size: 1901 724 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 949 2625 Size: 949 1676 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1130 1165 2625 Size: 1130 35 1460 Fragments arranged by size: 1460 1130 35 DraI TTT'AAA Cuts at: 0 366 1985 2625 Size: 366 1619 640 Fragments arranged by size: 1619 640 366 EarI CTCTTCn'nnn_ Cuts at: 0 383 456 545 1187 1334 2416 2625 Size: 383 73 89 642 147 1082 209 Fragments arranged by size: 1082 642 383 209 147 89 73 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1210 2625 Size: 1210 1415 EcoNI CCTnn'n_nnAGG Cuts at: 0 2450 2625 Size: 2450 175 EcoO109I rG'GnC_Cy Cuts at: 0 682 1264 2625 Size: 682 582 1361 Fragments arranged by size: 1361 682 582 EcoRI G'AATT_C Cuts at: 0 1493 2625 Size: 1493 1132 EcoRV GAT'ATC Cuts at: 0 2045 2625 Size: 2045 580 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 299 2587 2625 Size: 299 2288 38 Fragments arranged by size: 2288 299 38 HaeII r_GCGC'y Cuts at: 0 353 2625 Size: 353 2272 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 679 711 2067 2099 2625 Size: 679 32 1356 32 526 Fragments arranged by size: 1356 679 526 32 32 HincII GTy'rAC Cuts at: 0 119 2625 Size: 119 2506 HpaI GTT'AAC Cuts at: 0 119 2625 Size: 119 2506 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 196 2625 Size: 196 2429 MspA1I CmG'CkG Cuts at: 0 82 1728 2625 Size: 82 1646 897 Fragments arranged by size: 1646 897 82 NcoI C'CATG_G Cuts at: 0 1901 2625 Size: 1901 724 NdeI CA'TA_TG Cuts at: 0 2213 2625 Size: 2213 412 NheI G'CTAG_C Cuts at: 0 61 2625 Size: 61 2564 NspI r_CATG'y Cuts at: 0 593 1439 1503 2622 2625 Size: 593 846 64 1119 3 Fragments arranged by size: 1119 846 593 64 3 PciI A'CATG_T Cuts at: 0 1435 2625 Size: 1435 1190 PflMI CCAn_nnn'nTGG Cuts at: 0 1759 2625 Size: 1759 866 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1408 1440 2625 Size: 1408 32 1185 Fragments arranged by size: 1408 1185 32 PpuMI rG'GwC_Cy Cuts at: 0 1264 2625 Size: 1264 1361 PshAI GACnn'nnGTC Cuts at: 0 981 2625 Size: 981 1644 PsiI TTA'TAA Cuts at: 0 227 1046 2625 Size: 227 819 1579 Fragments arranged by size: 1579 819 227 PspXI vC'TCGA_Gb Cuts at: 0 217 1268 2625 Size: 217 1051 1357 Fragments arranged by size: 1357 1051 217 PstI C_TGCA'G Cuts at: 0 1647 2625 Size: 1647 978 PvuII CAG'CTG Cuts at: 0 82 1728 2625 Size: 82 1646 897 Fragments arranged by size: 1646 897 82 SapI GCTCTTCn'nnn_ Cuts at: 0 1187 2625 Size: 1187 1438 ScaI AGT'ACT Cuts at: 0 1853 2242 2625 Size: 1853 389 383 Fragments arranged by size: 1853 389 383 SfcI C'TryA_G Cuts at: 0 1530 1643 1911 2318 2625 Size: 1530 113 268 407 307 Fragments arranged by size: 1530 407 307 268 113 SmlI C'TyrA_G Cuts at: 0 217 289 633 833 1268 2556 2625 Size: 217 72 344 200 435 1288 69 Fragments arranged by size: 1288 435 344 217 200 72 69 SphI G_CATG'C Cuts at: 0 2622 2625 Size: 2622 3 StyI C'CwwG_G Cuts at: 0 521 960 1901 2625 Size: 521 439 941 724 Fragments arranged by size: 941 724 521 439 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1979 2625 Size: 1979 646 TatI w'GTAC_w Cuts at: 0 1502 1851 1890 2240 2625 Size: 1502 349 39 350 385 Fragments arranged by size: 1502 385 350 349 39 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1145 1177 2625 Size: 1145 32 1448 Fragments arranged by size: 1448 1145 32 XbaI T'CTAG_A Cuts at: 0 1371 2292 2625 Size: 1371 921 333 Fragments arranged by size: 1371 921 333 XhoI C'TCGA_G Cuts at: 0 217 833 1268 2625 Size: 217 616 435 1357 Fragments arranged by size: 1357 616 435 217 XmnI GAAnn'nnTTC Cuts at: 0 856 1420 1721 2378 2625 Size: 856 564 301 657 247 Fragments arranged by size: 856 657 564 301 247 Enzymes that do cut: AccI AcuI AflIII ApoI AseI AvaI BanI BanII BbsI BbvCI BclI BglII BlpI Bme1580I BmgBI BmrI BmtI Bpu10I BpuEI BsaAI BsaBI BsaWI BsaXI BsgI BsiHKAI BsmI Bsp1286I BspEI BsrDI BsrGI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI CspCI DraI EarI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HpaI MmeI MspA1I NcoI NdeI NheI NspI PciI PflMI PpiI PpuMI PshAI PsiI PspXI PstI PvuII SapI ScaI SfcI SmlI SphI StyI TaqII TatI TstI XbaI XhoI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI AsiSI AvrII BaeI BamHI BbeI BcgI BciVI BfrBI BglI BplI BpmI BsaI BsaHI BseRI BseYI BsiEI BsiWI BsmBI BspHI BspMI BsrBI BsrFI BssHII BssSI BstXI Bsu36I BtsI ClaI DraIII DrdI EaeI EagI EciI EcoICRI FseI FspI FspAI HindIII KasI KpnI MfeI MluI MscI MslI NaeI NarI NgoMIV NotI NruI NsiI PacI PasI PfoI PmeI PmlI PspOMI PsrI PvuI RsrII SacI SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI Tth111I XcmI XmaI ZraI