################################## # NO CUT ENZYMES ( 198 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AgeI AhdI Alw44I AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstF5I BstPI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DraIII DsaI EaeI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FokI FseI FspI HaeIII HapII HgaI Hin1I HincII HindII HinfI HpaI HpaII HphI KasI KpnI Ksp632I KspI MamI MboII MfeI MflI MluI MluNI MroI MscI MseI MslI MspI MunI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tru9I Tsp509I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccII 117 CGCG CG^CG AfaI 252 GTAC GT^AC AflIII 254 ACGTGT A^C[GA][CT]GT AlwI 226 GATCC GGATC(4/5) AlwNI 165 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 60 AGCGCT AGC^GCT AvaII 243 GGTCC G^G[AT]CC BanII 78 GGGCTC G[GA]GC[CT]^C BfaI 37 CTAG C^TAG BmyI 78 GGGCTC G[AGT]GC[ACT]^C BpmI 56 CTCCAG CTGGAG(16/14) BsaAI 253 TACGTG [CT]AC^GT[GA] BsaJI 239 CCTGGG C^C[ACGT][ACGT]GG BseRI 28 CTCCTC GAGGAG(10/8) Bsp1286I 78 GGGCTC G[AGT]GC[ACT]^C BsrI 230 CCAGT ACTGG(1/-1) BstAPI 164 GCAGCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 117 CGCG CG^CG CfoI 61 GCGC GCG^C Cfr13I 243 GGTCC G^G[ACGT]CC DpnII 226 GATC ^GATC DrdI 15 GACTGTCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 60 AGCGCT AGC^GCT HaeII 60 AGCGCT [GA]GCGC^[CT] HhaI 61 GCGC GCG^C HinP1I 61 GCGC G^CGC HindIII 124 AAGCTT A^AGCTT MaeI 37 CTAG C^TAG MaeII 254 ACGT A^CGT MaeIII 13 GTGAC ^GT[ACGT]AC MboI 226 GATC ^GATC MspA1I 182 CAGCTG C[AC]G^C[GT]G MvnI 117 CGCG CG^CG NdeII 226 GATC ^GATC NlaIII 33 CATG CATG^ NlaIV 242 GGGTCC GG[ACGT]^[ACGT]CC PshAI 237 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 182 CAGCTG CAG^CTG RsaI 252 GTAC GT^AC Sau3AI 226 GATC ^GATC Sau96I 243 GGTCC G^G[ACGT]CC Tsp45I 13 GTGAC ^GT[GC]AC TspRI 231 CAGTG CA[GC]TG[ACGT][ACGT]^ ################################### # TWO CUT ENZYMES ( 4 enzymes) # ################################### AciI 95 CCGC CCGC(-3/-1) 116 CCGC BsaI 157 GAGACC GGTCTC(1/5) 235 GAGACC DdeI 66 CTCAG C^T[ACGT]AG 81 CTCAG MwoI 61 GCGCTCTCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 164 GCAGCCTCTGC ######################################### # 3 OR MORE CUT ENZYMES ( 13 enzymes) # ######################################### AluI BbvI BsmAI BssKI BstNI Cac8I EcoRII Fnu4HI ItaI MnlI MvaI ScrFI TseI