Restriction map for pFN21AE1584
 (Circular) (Six-base) MAPSORT of: pFN21AE1584.seq  Check: 7180  from: 1  to: 4981



pFN21AE1584  4981 bp

 With 182 enzymes: SgfI * 

                        July 6, 2012 17:38  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    2791     278 
   Size:         53      83     186     936    1255    2468

  Fragments arranged by size: 

                2468    1255     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2324    1937 
   Size:        387    4594
 
Acc65I G'GTAC_C

Cuts at:   2302    2302 
   Size:       4981
 
AclI AA'CG_TT

Cuts at:   1563    2280    3108    3481    1563 
   Size:        717     828     373    3063

  Fragments arranged by size: 

                3063     828     717     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3044    4428    4696    1632 
   Size:       1412    1384     268    1917

  Fragments arranged by size: 

                1917    1412    1384     268
 
AfeI AGC'GCT

Cuts at:   2067    2067 
   Size:       4981
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    4784
 
AflIII A'CryG_T

Cuts at:   1203    2259    3880    4857    1203 
   Size:       1056    1621     977    1327

  Fragments arranged by size: 

                1621    1327    1056     977
 
AgeI A'CCGG_T

Cuts at:   3812    3812 
   Size:       4981
 
AhdI GACnn_n'nnGTC

Cuts at:   3710    3710 
   Size:       4981
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       4981
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    2241    1184 
   Size:       1057    3924
 
AlwNI CAG_nnn'CTG

Cuts at:   2175    2724    4296    2175 
   Size:        549    1572    2860

  Fragments arranged by size: 

                2860    1572     549
 
ApaLI G'TGCA_C

Cuts at:   3038    4194    3038 
   Size:       1156    3825
 
ApoI r'AATT_y

Cuts at:   1493    2290    2516    2668    4777    1493 
   Size:        797     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     797     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3535     160 
   Size:        872    2503    1606

  Fragments arranged by size: 

                2503    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       4981
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2306    1870 
   Size:         73     363    4545

  Fragments arranged by size: 

                4545     363      73
 
BamHI G'GATC_C

Cuts at:   2311    2678    2311 
   Size:        367    4614
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2302    3757     618 
   Size:        325     217     627     515    1455    1842

  Fragments arranged by size: 

                1842    1455     627     515     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2087    2300    1391 
   Size:        696     213    4072

  Fragments arranged by size: 

                4072     696     213
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4010
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3172    3206    1903 
   Size:         34    1235      34    3678

  Fragments arranged by size: 

                3678    1235      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2873    4083    2873 
   Size:       1210    3771
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       4981
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    3592     136 
   Size:        107     122      71     631     605    1920    1525

  Fragments arranged by size: 

                1920    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   4976    4976 
   Size:       4981
 
BlpI GC'TnA_GC

Cuts at:   2398    2398 
   Size:       4981
 
Bme1580I G_kGCm'C

Cuts at:   1790    3042    4198    1790 
   Size:       1252    1156    2573

  Fragments arranged by size: 

                2573    1252    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       4981
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2229    2712    3670 
   Size:        416      53      83     399      24     812     483     958

Cuts at:   3670     442 
   Size:       1753

  Fragments arranged by size: 

                1753     958     812     483     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       4981
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2046    3641    1239 
   Size:        807    1595    2579

  Fragments arranged by size: 

                2579    1595     807
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3106    3971    4269    3106 
   Size:        865     298    3818

  Fragments arranged by size: 

                3818     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2156    2234    3644     882 
   Size:        617     657      78    1410    2219

  Fragments arranged by size: 

                2219    1410     657     617      78
 
BsaAI yAC'GTr

Cuts at:    493    1204    2260    2772     493 
   Size:        711    1056     512    2702

  Fragments arranged by size: 

                2702    1056     711     512
 
BsaBI GATnn'nnATC

Cuts at:   4975    4975 
   Size:       4981
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2788    3170    3805     275 
   Size:         53      83     186     936    1255     382     635    1451

  Fragments arranged by size: 

                1451    1255     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3414    3812    4086    4233    4644    3414 
   Size:        398     274     147     411    3751

  Fragments arranged by size: 

                3751     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2246    2276    1178 
   Size:         30     165      30     843      30    3883

  Fragments arranged by size: 

                3883     843     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2024    1186 
   Size:        838    4143
 
BseYI C'CCAG_C

Cuts at:   2181    4184    4680    2181 
   Size:       2003     496    2482

  Fragments arranged by size: 

                2482    2003     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4408
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2431    3192    4220    1540 
   Size:        275     186     430     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     430     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3042    3127    4198    3042 
   Size:         85    1071    3825

  Fragments arranged by size: 

                3825    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2492    2585    2492 
   Size:         93    4888
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2087    2300    3042    3127    4198    1391 
   Size:        399     297     213     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     399     297     213      85
 
BspEI T'CCGG_A

Cuts at:   4644    4644 
   Size:       4981
 
BspHI T'CATG_A

Cuts at:   2870    4600    2870 
   Size:       1730    3251
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2336     844 
   Size:       1492    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2868    1394 
   Size:        429    1045    3507

  Fragments arranged by size: 

                3507    1045     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3476    3650      65 
   Size:       1183    2228     174    1396

  Fragments arranged by size: 

                2228    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3625    3812    1700 
   Size:        110    1815     187    2869

  Fragments arranged by size: 

                2869    1815     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       4981
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       4981
 
BssSI C'ACGA_G

Cuts at:   3041    4053    3041 
   Size:       1012    3969
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2175     794 
   Size:       1381    3600
 
BstBI TT'CG_AA

Cuts at:   3785    3785 
   Size:       4981
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       4981
 
BstYI r'GATC_y

Cuts at:   1972    2311    2678    3063    3080    4532    4618    4630    4976 
   Size:        339     367     385      17    1452      86      12     346

Cuts at:   4976    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452     385     367     346     339      86      17
                  12
 
BtgI C'CryG_G

Cuts at:    513    1065     513 
   Size:        552    4429
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4242

  Fragments arranged by size: 

                4242     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2497    3291    3311    2497 
   Size:        794      20    4167

  Fragments arranged by size: 

                4167     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       4981
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    4946
 
DraI TTT'AAA

Cuts at:   2285    2285 
   Size:       4981
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    2026    3988     809 
   Size:        507     710    1962    1802

  Fragments arranged by size: 

                1962    1802     710     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2428    3317    4946 
   Size:         54    1425      50     275      18     598     889    1629

Cuts at:   4946       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425     889     598     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2428    1537 
   Size:        275     616    4090

  Fragments arranged by size: 

                4090     616     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2911    1353 
   Size:       1558    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3568    3942    4088     387 
   Size:       1064     138    1979     374     146    1280

  Fragments arranged by size: 

                1979    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2046    3044    3641    4428    4696    1239 
   Size:        393     414     998     597     787     268    1524

  Fragments arranged by size: 

                1524     998     787     597     414     393     268
 
EcoRI G'AATT_C

Cuts at:   2290    2668    2290 
   Size:        378    4603
 
FspI TGC'GCA

Cuts at:   3487    3487 
   Size:       4981
 
HaeII r_GCGC'y

Cuts at:   1678    2069    4128    1678 
   Size:        391    2059    2531

  Fragments arranged by size: 

                2531    2059     391
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    3622    3654    3696    3728    1373 
   Size:         32     135      32    2050      32      42      32    2626

  Fragments arranged by size: 

                2626    2050     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2325    2571     669 
   Size:        988     281     387     246    3079

  Fragments arranged by size: 

                3079     988     387     281     246
 
HindIII A'AGCT_T

Cuts at:    748    2129     748 
   Size:       1381    3600
 
HpaI GTT'AAC

Cuts at:   2571    2571 
   Size:       4981
 
KpnI G_GTAC'C

Cuts at:   2306    2306 
   Size:       4981
 
MfeI C'AATT_G

Cuts at:   2580    2580 
   Size:       4981
 
MluI A'CGCG_T

Cuts at:   4857    4857 
   Size:       4981
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4095    4279    1305 
   Size:       2790     184    2007

  Fragments arranged by size: 

                2790    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3159

  Fragments arranged by size: 

                3159    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2939    3298    3457     518 
   Size:        648    1773     359     159    2042

  Fragments arranged by size: 

                2042    1773     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2189    2397    3074    4222    4467    1606 
   Size:        583     208     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     677     583     245     208
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    4429
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       4981
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       4981
 
NotI GC'GGCC_GC

Cuts at:   2428    2428 
   Size:       4981
 
NspI r_CATG'y

Cuts at:   1132    1229    2339    3884    1132 
   Size:         97    1110    1545    2229

  Fragments arranged by size: 

                2229    1545    1110      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       4981
 
PciI A'CATG_T

Cuts at:   3880    3880 
   Size:       4981
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       4981
 
PfoI T'CCnGG_A

Cuts at:   2250    2250 
   Size:       4981
 
PmeI GTTT'AAAC

Cuts at:   2285    2285 
   Size:       4981
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3050    3082    4563    4595    3050 
   Size:         32    1481      32    3436

  Fragments arranged by size: 

                3436    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    2246    1613 
   Size:        633    4348
 
PsiI TTA'TAA

Cuts at:   2551    2551 
   Size:       4981
 
PstI C_TGCA'G

Cuts at:    830    2333     830 
   Size:       1503    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       4981
 
PvuII CAG'CTG

Cuts at:   2189    2189 
   Size:       4981
 
SalI G'TCGA_C

Cuts at:   1936    2323    1936 
   Size:        387    4594
 
SbfI CC_TGCA'GG

Cuts at:   2333    2333 
   Size:       4981
 
ScaI AGT'ACT

Cuts at:   3229    4941    3229 
   Size:       1712    3269
 
SfcI C'TryA_G

Cuts at:    826    1045    2329    3464    4145    4336     826 
   Size:        219    1284    1135     681     191    1471

  Fragments arranged by size: 

                1471    1284    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       4981
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       4981
 
SmaI CCC'GGG

Cuts at:   1872    2308    1872 
   Size:        436    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3085    3986    4248     820 
   Size:        197     926    1142     901     262    1553

  Fragments arranged by size: 

                1553    1142     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    2772     493 
   Size:       2279    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       4981
 
SphI G_CATG'C

Cuts at:   1132    2339    1132 
   Size:       1207    3774
 
SspI AAT'ATT

Cuts at:      5      52    2905       5 
   Size:         47    2853    2081

  Fragments arranged by size: 

                2853    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       4981
 
StyI C'CwwG_G

Cuts at:    513    1065    2420     513 
   Size:        552    1355    3074

  Fragments arranged by size: 

                3074    1355     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3008    3025    3178    3363    3808     325 
   Size:       1255    1428      17     153     185     445    1498

  Fragments arranged by size: 

                1498    1428    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3227    4939      96 
   Size:        274      80      33      51    1443    1250    1712     138

  Fragments arranged by size: 

                1712    1443    1250     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   4941    4973    4941 
   Size:         32    4949
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       4981
 
XbaI T'CTAG_A

Cuts at:   2317    2317 
   Size:       4981
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       4981
 
XmaI C'CCGG_G

Cuts at:   1870    2306    1870 
   Size:        436    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3110    3110 
   Size:       4981
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    2789     276 
   Size:         53      83     186     936    1255    2468

  Fragments arranged by size: 

                2468    1255     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI  Eco57MI
    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NcoI     NdeI
     NheI     NotI     NspI     PasI     PciI    PflMI     PfoI     PmeI
     PpiI    PshAI     PsiI     PstI     PvuI    PvuII     SalI     SbfI
     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AloI     ApaI     AscI    AvrII     BaeI     BbeI    BbvCI
    BfrBI     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I
   DraIII  EcoICRI    EcoNI EcoO109I    EcoRV     FalI     FseI    FspAI
     KasI     NaeI     NarI   NgoMIV     NruI     NsiI     PacI     PmlI
    PpuMI   PspOMI    PspXI     PsrI    RsrII     SacI    SacII    SanDI
     SapI    SexAI     SfiI     SfoI     SrfI     SwaI     XcmI
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