(Circular) (Six-base) MAPSORT of: pFN21AE3369.seq Check: 6564 from: 1 to: 7972 pFN21AE3369 7972 bp With 182 enzymes: SgfI * November 11, 2016 17:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5782 278 Size: 53 83 186 936 4246 2468 Fragments arranged by size: 4246 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4779 5315 1937 Size: 2842 536 4594 Fragments arranged by size: 4594 2842 536 Acc65I G'GTAC_C Cuts at: 2506 4740 5293 2506 Size: 2234 553 5185 Fragments arranged by size: 5185 2234 553 AclI AA'CG_TT Cuts at: 1563 6099 6472 1563 Size: 4536 373 3063 Fragments arranged by size: 4536 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2211 2950 3336 3566 3959 4934 5024 5160 Size: 579 739 386 230 393 975 90 136 Cuts at: 5160 5229 6035 7419 7687 1632 Size: 69 806 1384 268 1917 Fragments arranged by size: 1917 1384 975 806 739 579 393 386 268 230 136 90 69 AfeI AGC'GCT Cuts at: 5044 5044 Size: 7972 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7775 AflIII A'CryG_T Cuts at: 1203 6871 7848 1203 Size: 5668 977 1327 Fragments arranged by size: 5668 1327 977 AgeI A'CCGG_T Cuts at: 6803 6803 Size: 7972 AhdI GACnn_n'nnGTC Cuts at: 6701 6701 Size: 7972 AleI CACnn'nnGTG Cuts at: 1166 2493 3101 4842 1166 Size: 1327 608 1741 4296 Fragments arranged by size: 4296 1741 1327 608 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2798 1184 Size: 1614 6358 AlwNI CAG_nnn'CTG Cuts at: 5038 5715 7287 5038 Size: 677 1572 5723 Fragments arranged by size: 5723 1572 677 ApaI G_GGCC'C Cuts at: 2562 2562 Size: 7972 ApaLI G'TGCA_C Cuts at: 3307 6029 7185 3307 Size: 2722 1156 4094 Fragments arranged by size: 4094 2722 1156 ApoI r'AATT_y Cuts at: 1493 2128 3672 4896 5281 5507 5659 7768 1493 Size: 635 1544 1224 385 226 152 2109 1697 Fragments arranged by size: 2109 1697 1544 1224 635 385 226 152 AseI AT'TA_AT Cuts at: 160 1032 6526 160 Size: 872 5494 1606 Fragments arranged by size: 5494 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7972 AvaI C'yCGr_G Cuts at: 1870 1943 2468 2837 3438 4413 5297 1870 Size: 73 525 369 601 975 884 4545 Fragments arranged by size: 4545 975 884 601 525 369 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3433 3466 3433 Size: 33 7939 BamHI G'GATC_C Cuts at: 5302 5669 5302 Size: 367 7605 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2506 4682 4740 5293 6748 Size: 325 217 627 719 2176 58 553 1455 Cuts at: 6748 618 Size: 1842 Fragments arranged by size: 2176 1842 1455 719 627 553 325 217 58 BanII G_rGCy'C Cuts at: 1391 2204 2562 5291 1391 Size: 813 358 2729 4072 Fragments arranged by size: 4072 2729 813 358 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2036 3171 5001 5214 5265 928 Size: 971 137 1135 1830 213 51 3635 Fragments arranged by size: 3635 1830 1135 971 213 137 51 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2858 2892 3724 3758 6163 6197 1903 Size: 34 921 34 832 34 2405 34 3678 Fragments arranged by size: 3678 2405 921 832 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5864 7074 5864 Size: 1210 6762 BclI T'GATC_A Cuts at: 1548 3271 3618 1548 Size: 1723 347 5902 Fragments arranged by size: 5902 1723 347 BfrBI ATG'CAT Cuts at: 4537 4964 4537 Size: 427 7545 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2279 2885 6583 Size: 107 122 71 631 605 607 606 3698 Cuts at: 6583 136 Size: 1525 Fragments arranged by size: 3698 1525 631 607 606 605 122 107 71 BglII A'GATC_T Cuts at: 4503 4665 7967 4503 Size: 162 3302 4508 Fragments arranged by size: 4508 3302 162 BlpI GC'TnA_GC Cuts at: 2057 5389 2057 Size: 3332 4640 Bme1580I G_kGCm'C Cuts at: 1790 2562 3311 6033 7189 1790 Size: 772 749 2722 1156 2573 Fragments arranged by size: 2722 2573 1156 772 749 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7972 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3297 5020 5703 Size: 416 53 83 399 24 1880 1723 683 Cuts at: 5703 6661 442 Size: 958 1753 Fragments arranged by size: 1880 1753 1723 958 683 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7972 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3451 3451 Size: 7972 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2775 3700 3828 6632 1239 Size: 1536 925 128 2804 2579 Fragments arranged by size: 2804 2579 1536 925 128 Bpu10I CC'TnA_GC Cuts at: 3156 4085 3156 Size: 929 7043 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3222 3739 6097 6962 7260 3222 Size: 517 2358 865 298 3934 Fragments arranged by size: 3934 2358 865 517 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3482 4816 5192 6635 882 Size: 617 1983 1334 376 1443 2219 Fragments arranged by size: 2219 1983 1443 1334 617 376 BsaAI yAC'GTr Cuts at: 493 1204 5763 493 Size: 711 4559 2702 Fragments arranged by size: 4559 2702 711 BsaBI GATnn'nnATC Cuts at: 3617 7966 3617 Size: 4349 3623 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5779 6161 6796 275 Size: 53 83 186 936 4246 382 635 1451 Fragments arranged by size: 4246 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4743 6405 6803 7077 7224 7635 4743 Size: 1662 398 274 147 411 5080 Fragments arranged by size: 5080 1662 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4035 4065 1178 Size: 30 165 30 2632 30 5085 Fragments arranged by size: 5085 2632 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3500 5108 5111 1186 Size: 2314 1608 3 4047 Fragments arranged by size: 4047 2314 1608 3 BseYI C'CCAG_C Cuts at: 3985 7175 7671 3985 Size: 3190 496 4286 Fragments arranged by size: 4286 3190 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2141 3979 1163 Size: 573 405 1838 5156 Fragments arranged by size: 5156 1838 573 405 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5422 6183 7211 1540 Size: 275 186 3421 761 1028 2301 Fragments arranged by size: 3421 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2204 3311 6033 6118 7189 2204 Size: 1107 2722 85 1071 2987 Fragments arranged by size: 2987 2722 1107 1071 85 BsmI GAATG_Cn' Cuts at: 4650 5483 5576 4650 Size: 833 93 7046 Fragments arranged by size: 7046 833 93 BsmBI CGTCTCn'nnnn_ Cuts at: 5150 5150 Size: 7972 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2204 2562 3311 5291 6033 6118 7189 Size: 399 414 358 749 1980 742 85 1071 Cuts at: 7189 1391 Size: 2174 Fragments arranged by size: 2174 1980 1071 749 742 414 399 358 85 BspEI T'CCGG_A Cuts at: 7635 7635 Size: 7972 BspHI T'CATG_A Cuts at: 3867 5861 7591 3867 Size: 1994 1730 4248 Fragments arranged by size: 4248 1994 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2518 2997 5327 844 Size: 1674 479 2330 3489 Fragments arranged by size: 3489 2330 1674 479 BsrBI CCG'CTC Cuts at: 1394 1823 5859 1394 Size: 429 4036 3507 Fragments arranged by size: 4036 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6467 6641 65 Size: 1183 5219 174 1396 Fragments arranged by size: 5219 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3852 6616 6803 1700 Size: 110 2042 2764 187 2869 Fragments arranged by size: 2869 2764 2042 187 110 BsrGI T'GTAC_A Cuts at: 96 2295 2820 96 Size: 2199 525 5248 Fragments arranged by size: 5248 2199 525 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7972 BssSI C'ACGA_G Cuts at: 6032 7044 6032 Size: 1012 6960 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7972 BstBI TT'CG_AA Cuts at: 4544 6776 4544 Size: 2232 5740 BstEII G'GTnAC_C Cuts at: 1184 2701 1184 Size: 1517 6455 BstXI CCAn_nnnn'nTGG Cuts at: 2815 3103 2815 Size: 288 7684 BstYI r'GATC_y Cuts at: 1972 2647 3429 3905 4503 4665 5302 5669 6054 Size: 675 782 476 598 162 637 367 385 Cuts at: 6054 6071 7523 7609 7621 7967 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 782 675 637 598 476 385 367 346 162 86 17 12 Bsu36I CC'TnA_GG Cuts at: 5091 5091 Size: 7972 BtgI C'CryG_G Cuts at: 513 1065 2004 2844 3277 3453 3897 513 Size: 552 939 840 433 176 444 4588 Fragments arranged by size: 4588 939 840 552 444 433 176 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3478 3517 487 Size: 683 56 2252 39 4942 Fragments arranged by size: 4942 2252 683 56 39 BtsI GCAGTG_nn' Cuts at: 2275 3885 5488 6282 6302 2275 Size: 1610 1603 794 20 3945 Fragments arranged by size: 3945 1610 1603 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7972 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7937 DraI TTT'AAA Cuts at: 2628 5276 2628 Size: 2648 5324 DraIII CAC_nnn'GTG Cuts at: 4631 4631 Size: 7972 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4334 6979 809 Size: 507 3018 2645 1802 Fragments arranged by size: 3018 2645 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3172 3399 3900 Size: 54 1425 50 275 18 1342 227 501 Cuts at: 3900 4521 4749 5419 6308 7937 8 Size: 621 228 670 889 1629 43 Fragments arranged by size: 1629 1425 1342 889 670 621 501 275 228 227 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5419 1537 Size: 275 3607 4090 Fragments arranged by size: 4090 3607 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2190 2322 3206 4814 4910 5136 5248 5902 Size: 837 132 884 1608 96 226 112 654 Cuts at: 5902 1353 Size: 3423 Fragments arranged by size: 3423 1608 884 837 654 226 132 112 96 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6559 6933 7079 387 Size: 1064 138 4970 374 146 1280 Fragments arranged by size: 4970 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2202 2202 Size: 7972 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2211 2775 2950 3336 3566 3700 3828 Size: 393 579 564 175 386 230 134 128 Cuts at: 3828 3959 4934 5024 5160 5229 6035 6632 7419 Size: 131 975 90 136 69 806 597 787 Cuts at: 7419 7687 1239 Size: 268 1524 Fragments arranged by size: 1524 975 806 787 597 579 564 393 386 268 230 175 136 134 131 128 90 69 EcoO109I rG'GnC_Cy Cuts at: 2544 2558 2559 4007 5101 2544 Size: 14 1 1448 1094 5415 Fragments arranged by size: 5415 1448 1094 14 1 EcoRI G'AATT_C Cuts at: 2128 5281 5659 2128 Size: 3153 378 4441 Fragments arranged by size: 4441 3153 378 EcoRV GAT'ATC Cuts at: 3045 4123 3045 Size: 1078 6894 FspI TGC'GCA Cuts at: 6478 6478 Size: 7972 HaeII r_GCGC'y Cuts at: 1678 5046 7119 1678 Size: 3368 2073 2531 Fragments arranged by size: 3368 2531 2073 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2002 2034 3064 3096 3934 Size: 32 135 32 430 32 1030 32 838 Cuts at: 3934 3966 4035 4067 4346 4378 4625 4657 4909 Size: 32 69 32 279 32 247 32 252 Cuts at: 4909 4941 6613 6645 6687 6719 1373 Size: 32 1672 32 42 32 2626 Fragments arranged by size: 2626 1672 1030 838 430 279 252 247 135 69 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2734 2986 3234 4054 5316 5562 Size: 988 281 796 252 248 820 1262 246 Cuts at: 5562 669 Size: 3079 Fragments arranged by size: 3079 1262 988 820 796 281 252 248 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7972 HpaI GTT'AAC Cuts at: 3234 5562 3234 Size: 2328 5644 KpnI G_GTAC'C Cuts at: 2510 4744 5297 2510 Size: 2234 553 5185 Fragments arranged by size: 5185 2234 553 MfeI C'AATT_G Cuts at: 5571 5571 Size: 7972 MluI A'CGCG_T Cuts at: 7848 7848 Size: 7972 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2955 4293 7086 7270 1305 Size: 1650 1338 2793 184 2007 Fragments arranged by size: 2793 2007 1650 1338 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3174 3401 4523 4751 10 Size: 54 1425 343 1342 227 1122 228 3231 Fragments arranged by size: 3231 1425 1342 1122 343 228 227 54 MslI CAynn'nnrTG Cuts at: 518 1166 2363 2493 2780 3101 3497 4202 4703 Size: 648 1197 130 287 321 396 705 501 Cuts at: 4703 4842 4946 5216 5930 6289 6448 518 Size: 139 104 270 714 359 159 2042 Fragments arranged by size: 2042 1197 714 705 648 501 396 359 321 287 270 159 139 130 104 MspA1I CmG'CkG Cuts at: 1606 2125 2238 3314 3742 5388 6065 7213 7458 Size: 519 113 1076 428 1646 677 1148 245 Cuts at: 7458 1606 Size: 2120 Fragments arranged by size: 2120 1646 1148 1076 677 519 428 245 113 NcoI C'CATG_G Cuts at: 513 1065 2004 3277 513 Size: 552 939 1273 5208 Fragments arranged by size: 5208 1273 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7972 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7972 NotI GC'GGCC_GC Cuts at: 5419 5419 Size: 7972 NsiI A_TGCA'T Cuts at: 4539 4966 4539 Size: 427 7545 NspI r_CATG'y Cuts at: 1132 1229 4945 5330 6875 1132 Size: 97 3716 385 1545 2229 Fragments arranged by size: 3716 2229 1545 385 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7972 PciI A'CATG_T Cuts at: 6871 6871 Size: 7972 PflMI CCAn_nnn'nTGG Cuts at: 1108 3776 1108 Size: 2668 5304 PfoI T'CCnGG_A Cuts at: 3386 3386 Size: 7972 PmeI GTTT'AAAC Cuts at: 5276 5276 Size: 7972 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6041 6073 7554 7586 6041 Size: 32 1481 32 6427 Fragments arranged by size: 6427 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2544 5101 2544 Size: 2557 5415 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7972 PsiI TTA'TAA Cuts at: 5542 5542 Size: 7972 PspOMI G'GGCC_C Cuts at: 2558 2558 Size: 7972 PstI C_TGCA'G Cuts at: 830 3595 5324 830 Size: 2765 1729 3478 Fragments arranged by size: 3478 2765 1729 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7972 PvuII CAG'CTG Cuts at: 2125 3314 3742 2125 Size: 1189 428 6355 Fragments arranged by size: 6355 1189 428 SacI G_AGCT'C Cuts at: 2204 2204 Size: 7972 SalI G'TCGA_C Cuts at: 1936 5314 1936 Size: 3378 4594 SapI GCTCTTCn'nnn_ Cuts at: 3206 3206 Size: 7972 SbfI CC_TGCA'GG Cuts at: 5324 5324 Size: 7972 ScaI AGT'ACT Cuts at: 6220 7932 6220 Size: 1712 6260 SexAI A'CCwGG_T Cuts at: 5250 5250 Size: 7972 SfcI C'TryA_G Cuts at: 826 1045 2553 3591 5320 6455 7136 7327 826 Size: 219 1508 1038 1729 1135 681 191 1471 Fragments arranged by size: 1729 1508 1471 1135 1038 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7972 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7972 SmaI CCC'GGG Cuts at: 1872 5299 1872 Size: 3427 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3237 3754 6076 6977 7239 820 Size: 197 926 1294 517 2322 901 262 1553 Fragments arranged by size: 2322 1553 1294 926 901 517 262 197 SnaBI TAC'GTA Cuts at: 493 5763 493 Size: 5270 2702 SpeI A'CTAG_T Cuts at: 152 3781 152 Size: 3629 4343 SphI G_CATG'C Cuts at: 1132 4945 5330 1132 Size: 3813 385 3774 Fragments arranged by size: 3813 3774 385 SspI AAT'ATT Cuts at: 5 52 4265 5896 5 Size: 47 4213 1631 2081 Fragments arranged by size: 4213 2081 1631 47 StuI AGG'CCT Cuts at: 1517 4371 1517 Size: 2854 5118 StyI C'CwwG_G Cuts at: 513 1065 2004 3277 3523 4675 5411 513 Size: 552 939 1273 246 1152 736 3074 Fragments arranged by size: 3074 1273 1152 939 736 552 246 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4110 5999 6016 6169 6354 6799 325 Size: 1255 2530 1889 17 153 185 445 1498 Fragments arranged by size: 2530 1889 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2295 2820 6218 Size: 274 80 33 51 1443 318 525 3398 Cuts at: 6218 7930 96 Size: 1712 138 Fragments arranged by size: 3398 1712 1443 525 318 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2449 2481 3285 3313 3317 3345 3618 3650 4215 Size: 32 804 28 4 28 273 32 565 Cuts at: 4215 4247 4482 4514 7932 7964 2449 Size: 32 235 32 3418 32 2457 Fragments arranged by size: 3418 2457 804 565 273 235 32 32 32 32 32 28 28 4 Tth111I GACn'n_nGTC Cuts at: 1316 3263 5084 1316 Size: 1947 1821 4204 Fragments arranged by size: 4204 1947 1821 XbaI T'CTAG_A Cuts at: 2650 5308 2650 Size: 2658 5314 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3259 3284 3259 Size: 25 7947 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7972 XmaI C'CCGG_G Cuts at: 1870 5297 1870 Size: 3427 4545 XmnI GAAnn'nnTTC Cuts at: 2942 3111 4896 6101 2942 Size: 169 1785 1205 4813 Fragments arranged by size: 4813 1785 1205 169 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5780 276 Size: 53 83 186 936 4246 2468 Fragments arranged by size: 4246 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BbeI BbvCI BsiWI BstZ17I EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI