Restriction map for
pFN21AE1798
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# NO CUT ENZYMES ( 103 enzymes) #
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AatII Acc65I AccI AclI AflII
AgeI AhdI AocI Aor51HI ApaI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII BbvCI BcgI
BfrI BglII BlpI Bpu1102I BsaBI
BsiEI BsiWI BsmI BsoBI BspDI
BspLU11I BspMI BsrBI BsrFI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI FseI FspI HpaI
KpnI MamI MfeI MluI MunI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I StuI SwaI Tth111I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Alw44I 1621 GTGCAC G^TGCAC
AlwNI 1729 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1621 GTGCAC G^TGCAC
AvrII 162 CCTAGG C^CTAGG
BamHI 1065 GGATCC G^GATCC
BbeI 28 GGCGCC GGCGC^C
BbrPI 917 CACGTG CAC^GTG
BbsI 1737 GAAGAC GAAGAC(2/6)
BciVI 1966 GTATCC GTATCC(6/5)
BclI 1467 TGATCA T^GATCA
BcnI 2370 CCCGG CC^[GC]GG
BglI 2212 GCCCCTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 162 CCTAGG C^CTAGG
BpuAI 1737 GAAGAC GAAGAC(2/6)
BsaAI 917 CACGTG [CT]AC^GT[GA]
BsaI 2374 GAGACC GGTCTC(1/5)
BsmFI 37 GGGAC GGGAC(10/14)
Bsp1407I 1835 TGTACA T^GTACA
BsrDI 1234 GCAATG GCAATG(2/0)
BsrGI 1835 TGTACA T^GTACA
BssSI 88 CACGAG CACGAG(-5/-1)
DsaI 32 CCGCGG C^C[GA][CT]GG
EcoRV 798 GATATC GAT^ATC
EcoT22I 1603 ATGCAT ATGCA^T
FbaI 1467 TGATCA T^GATCA
HaeII 28 GGCGCC [GA]GCGC^[CT]
HincII 706 GTTGAC GT[CT]^[GA]AC
HindII 706 GTTGAC GT[CT]^[GA]AC
KasI 28 GGCGCC G^GCGCC
KspI 32 CCGCGG CCGC^GG
NarI 28 GGCGCC GG^CGCC
NciI 2370 CCCGG CC^[GC]GG
NsiI 1603 ATGCAT ATGCA^T
PflMI 504 CCACAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 917 CACGTG CAC^GTG
PmlI 917 CACGTG CAC^GTG
PpuMI 158 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 2338 CTGCAG CTGCA^G
SacII 32 CCGCGG CCGC^GG
SfcI 2338 CTGCAG C^T[GA][CT]AG
SfoI 28 GGCGCC GGC^GCC
SspBI 1835 TGTACA T^GTACA
Van91I 504 CCACAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 893 CCAAGAAGAGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 32 enzymes) #
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AccIII 2100 TCCGGA T^CCGGA
2119 TCCGGA
AcyI 28 GGCGCC G[GA]^CG[CT]C
1825 GGCGTC
AflIII 1478 ACACGT A^C[GA][CT]GT
1888 ACGTGT
Asp700I 1456 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1539 GAAGCACTTC
BalI 210 TGGCCA TGG^CCA
564 TGGCCA
BanI 28 GGCGCC G^G[CT][GA]CC
660 GGCACC
BanII 148 GAGCTC G[GA]GC[CT]^C
1774 GAGCTC
BsaHI 28 GGCGCC G[GA]^CG[CT]C
1825 GGCGTC
BseAI 2100 TCCGGA T^CCGGA
2119 TCCGGA
BsgI 995 CTGCAC GTGCAG(16/14)
460 GTGCAG
BsiYI 504 CCACAAGTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
2385 CCAGTTCTCGG
BslI 504 CCACAAGTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
2385 CCAGTTCTCGG
BsmBI 1552 GAGACG CGTCTC(1/5)
2260 GAGACG
BspEI 2100 TCCGGA T^CCGGA
2119 TCCGGA
BstAPI 87 GCACGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
453 GCATGAGGTGC
BstXI 213 CCATGATACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1895 CCAGCGCGGTGG
DraII 158 AGGTCCT [GA]G^G[ACGT]CC[CT]
2210 AGGCCCC
EaeI 210 TGGCCA [CT]^GGCC[GA]
564 TGGCCA
EcoO109I 158 AGGTCCT [GA]G^G[ACGT]CC[CT]
2210 AGGCCCC
EcoT14I 162 CCTAGG C^C[AT][AT]GG
1992 CCAAGG
FauI 1081 CCCGC CCCGC(4/6)
34 GCGGG
Hin1I 28 GGCGCC G[GA]^CG[CT]C
1825 GGCGTC
MluNI 210 TGGCCA TGG^CCA
564 TGGCCA
MroI 2100 TCCGGA T^CCGGA
2119 TCCGGA
MscI 210 TGGCCA TGG^CCA
564 TGGCCA
NspI 117 ACATGC [GA]CATG^[CT]
1601 ACATGC
SacI 148 GAGCTC GAGCT^C
1774 GAGCTC
SfaNI 922 GATGC GCATC(5/9)
754 GCATC
SmlI 538 CTTGAG C^T[CT][GA]AG
2040 CTTGAG
StyI 162 CCTAGG C^C[AT][AT]GG
1992 CCAAGG
Tsp45I 1213 GTGAC ^GT[GC]AC
1808 GTGAC
XmnI 1456 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1539 GAAGCACTTC
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# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaII BbvI
BfaI BmyI BpmI BsaJI BsaWI
BseRI BsiHKAI BsmAI Bsp1286I BspHI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII EarI EcoRII Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NdeII NlaIII NlaIV PleI PvuII
RcaI RsaI Sau3AI Sau96I ScrFI
SspI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I XhoII