(Circular) (Six-base) MAPSORT of: pFN21AE2192.seq Check: 217 from: 1 to: 6427 pFN21AE2192 6427 bp With 182 enzymes: SgfI * July 6, 2012 17:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3640 3640 Size: 6427 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4237 278 Size: 53 83 186 936 2701 2468 Fragments arranged by size: 2701 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3770 1937 Size: 1833 4594 Acc65I G'GTAC_C Cuts at: 3748 3748 Size: 6427 AclI AA'CG_TT Cuts at: 1563 4554 4927 1563 Size: 2991 373 3063 Fragments arranged by size: 3063 2991 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2372 2662 3090 3207 3429 3681 4490 5874 Size: 740 290 428 117 222 252 809 1384 Cuts at: 5874 6142 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 809 740 428 290 268 252 222 117 AflII C'TTAA_G Cuts at: 820 1017 2411 820 Size: 197 1394 4836 Fragments arranged by size: 4836 1394 197 AflIII A'CryG_T Cuts at: 1203 5326 6303 1203 Size: 4123 977 1327 Fragments arranged by size: 4123 1327 977 AgeI A'CCGG_T Cuts at: 5258 5258 Size: 6427 AhdI GACnn_n'nnGTC Cuts at: 5156 5156 Size: 6427 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6427 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6427 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2749 2781 2749 Size: 32 6395 AlwNI CAG_nnn'CTG Cuts at: 2822 4170 5742 2822 Size: 1348 1572 3507 Fragments arranged by size: 3507 1572 1348 ApaLI G'TGCA_C Cuts at: 3653 4484 5640 3653 Size: 831 1156 4440 Fragments arranged by size: 4440 1156 831 ApoI r'AATT_y Cuts at: 1493 2968 3008 3624 3736 3962 4114 6223 1493 Size: 1475 40 616 112 226 152 2109 1697 Fragments arranged by size: 2109 1697 1475 616 226 152 112 40 AseI AT'TA_AT Cuts at: 160 1032 4981 160 Size: 872 3949 1606 Fragments arranged by size: 3949 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6427 AvaI C'yCGr_G Cuts at: 1870 1943 3752 1870 Size: 73 1809 4545 Fragments arranged by size: 4545 1809 73 BamHI G'GATC_C Cuts at: 3757 4124 3757 Size: 367 6060 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3748 5203 618 Size: 325 217 627 1961 1455 1842 Fragments arranged by size: 1961 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3746 1391 Size: 2355 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2312 3669 928 Size: 971 413 1357 3686 Fragments arranged by size: 3686 1357 971 413 BbvCI CC'TCA_GC Cuts at: 3450 3450 Size: 6427 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2535 2569 4618 4652 1903 Size: 34 598 34 2049 34 3678 Fragments arranged by size: 3678 2049 598 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3046 3450 3585 4319 5529 3046 Size: 404 135 734 1210 3944 Fragments arranged by size: 3944 1210 734 404 135 BclI T'GATC_A Cuts at: 1548 1548 Size: 6427 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5038 136 Size: 107 122 71 631 605 3366 1525 Fragments arranged by size: 3366 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6422 6422 Size: 6427 BlpI GC'TnA_GC Cuts at: 3844 3844 Size: 6427 Bme1580I G_kGCm'C Cuts at: 1790 2012 3657 4488 5644 1790 Size: 222 1645 831 1156 2573 Fragments arranged by size: 2573 1645 1156 831 222 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6427 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2025 4158 5116 Size: 416 53 83 399 24 608 2133 958 Cuts at: 5116 442 Size: 1753 Fragments arranged by size: 2133 1753 958 608 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6427 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2042 2904 3452 3493 5087 1239 Size: 803 862 548 41 1594 2579 Fragments arranged by size: 2579 1594 862 803 548 41 Bpu10I CC'TnA_GC Cuts at: 3450 3450 Size: 6427 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4552 5417 5715 4552 Size: 865 298 5264 Fragments arranged by size: 5264 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2891 3512 5090 882 Size: 617 1392 621 1578 2219 Fragments arranged by size: 2219 1578 1392 621 617 BsaAI yAC'GTr Cuts at: 493 1204 4218 493 Size: 711 3014 2702 Fragments arranged by size: 3014 2702 711 BsaBI GATnn'nnATC Cuts at: 6421 6421 Size: 6427 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4234 4616 5251 275 Size: 53 83 186 936 2701 382 635 1451 Fragments arranged by size: 2701 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4860 5258 5532 5679 6090 4860 Size: 398 274 147 411 5197 Fragments arranged by size: 5197 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6202 Fragments arranged by size: 6202 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3297 1186 Size: 2111 4316 BseYI C'CCAG_C Cuts at: 5630 6126 5630 Size: 496 5931 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5854 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3877 4638 5666 1540 Size: 275 186 1876 761 1028 2301 Fragments arranged by size: 2301 1876 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3657 4488 4573 5644 3657 Size: 831 85 1071 4440 Fragments arranged by size: 4440 1071 831 85 BsmI GAATG_Cn' Cuts at: 2252 3938 4031 2252 Size: 1686 93 4648 Fragments arranged by size: 4648 1686 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 3657 3746 4488 4573 5644 1391 Size: 399 222 1645 89 742 85 1071 2174 Fragments arranged by size: 2174 1645 1071 742 399 222 89 85 BspEI T'CCGG_A Cuts at: 6090 6090 Size: 6427 BspHI T'CATG_A Cuts at: 3346 3685 3706 4316 6046 3346 Size: 339 21 610 1730 3727 Fragments arranged by size: 3727 1730 610 339 21 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3640 3782 844 Size: 2796 142 3489 Fragments arranged by size: 3489 2796 142 BsrBI CCG'CTC Cuts at: 1394 1823 4314 1394 Size: 429 2491 3507 Fragments arranged by size: 3507 2491 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4922 5096 65 Size: 1183 3674 174 1396 Fragments arranged by size: 3674 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5071 5258 1700 Size: 110 3261 187 2869 Fragments arranged by size: 3261 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6427 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6427 BssSI C'ACGA_G Cuts at: 4487 5499 4487 Size: 1012 5415 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6427 BstBI TT'CG_AA Cuts at: 5231 5231 Size: 6427 BstEII G'GTnAC_C Cuts at: 1184 3312 1184 Size: 2128 4299 BstXI CCAn_nnnn'nTGG Cuts at: 2271 2271 Size: 6427 BstYI r'GATC_y Cuts at: 1972 2360 3757 4124 4509 4526 5978 6064 6076 Size: 388 1397 367 385 17 1452 86 12 Cuts at: 6076 6422 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1397 388 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2537 3394 2537 Size: 857 5570 BtgI C'CryG_G Cuts at: 513 1065 2004 2044 3353 513 Size: 552 939 40 1309 3587 Fragments arranged by size: 3587 1309 939 552 40 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2194 3352 487 Size: 683 56 968 1158 3562 Fragments arranged by size: 3562 1158 968 683 56 BtsI GCAGTG_nn' Cuts at: 2185 3572 3943 4737 4757 2185 Size: 1387 371 794 20 3855 Fragments arranged by size: 3855 1387 794 371 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6427 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6392 DraI TTT'AAA Cuts at: 3132 3731 3132 Size: 599 5828 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5434 809 Size: 507 4118 1802 Fragments arranged by size: 4118 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3874 4763 6392 Size: 54 1425 50 275 18 2044 889 1629 Cuts at: 6392 8 Size: 43 Fragments arranged by size: 2044 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3874 1537 Size: 275 2062 4090 Fragments arranged by size: 4090 2062 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2753 4357 1353 Size: 1400 1604 3423 Fragments arranged by size: 3423 1604 1400 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5014 5388 5534 387 Size: 1064 138 3425 374 146 1280 Fragments arranged by size: 3425 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2042 2372 2662 2904 3090 3207 3429 Size: 393 410 330 290 242 186 117 222 Cuts at: 3429 3452 3493 3681 4490 5087 5874 6142 1239 Size: 23 41 188 809 597 787 268 1524 Fragments arranged by size: 1524 809 787 597 410 393 330 290 268 242 222 188 186 117 41 23 EcoO109I rG'GnC_Cy Cuts at: 2541 2541 Size: 6427 EcoRI G'AATT_C Cuts at: 3736 4114 3736 Size: 378 6049 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3281 3356 3281 Size: 75 6352 FspI TGC'GCA Cuts at: 4933 4933 Size: 6427 HaeII r_GCGC'y Cuts at: 1678 2080 5574 1678 Size: 402 3494 2531 Fragments arranged by size: 3494 2531 402 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2017 2049 2485 2517 3292 Size: 32 135 32 445 32 436 32 775 Cuts at: 3292 3324 5068 5100 5142 5174 1373 Size: 32 1744 32 42 32 2626 Fragments arranged by size: 2626 1744 775 445 436 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2674 3771 4017 669 Size: 988 281 736 1097 246 3079 Fragments arranged by size: 3079 1097 988 736 281 246 HindIII A'AGCT_T Cuts at: 748 3359 748 Size: 2611 3816 HpaI GTT'AAC Cuts at: 2674 4017 2674 Size: 1343 5084 KpnI G_GTAC'C Cuts at: 3752 3752 Size: 6427 MfeI C'AATT_G Cuts at: 3579 4026 3579 Size: 447 5980 MluI A'CGCG_T Cuts at: 6303 6303 Size: 6427 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5541 5725 1305 Size: 4236 184 2007 Fragments arranged by size: 4236 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4605 Fragments arranged by size: 4605 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2269 2394 4385 4744 4903 518 Size: 648 1103 125 1991 359 159 2042 Fragments arranged by size: 2042 1991 1103 648 359 159 125 MspA1I CmG'CkG Cuts at: 1606 2046 3449 3843 4520 5668 5913 1606 Size: 440 1403 394 677 1148 245 2120 Fragments arranged by size: 2120 1403 1148 677 440 394 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3353 513 Size: 552 939 1349 3587 Fragments arranged by size: 3587 1349 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6427 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6427 NotI GC'GGCC_GC Cuts at: 3874 3874 Size: 6427 NspI r_CATG'y Cuts at: 1132 1229 2892 3785 5330 1132 Size: 97 1663 893 1545 2229 Fragments arranged by size: 2229 1663 1545 893 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6427 PciI A'CATG_T Cuts at: 5326 5326 Size: 6427 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6427 PmeI GTTT'AAAC Cuts at: 3731 3731 Size: 6427 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4496 4528 6009 6041 4496 Size: 32 1481 32 4882 Fragments arranged by size: 4882 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2541 2541 Size: 6427 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6427 PsiI TTA'TAA Cuts at: 3997 3997 Size: 6427 PstI C_TGCA'G Cuts at: 830 2239 2480 3779 830 Size: 1409 241 1299 3478 Fragments arranged by size: 3478 1409 1299 241 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6427 PvuII CAG'CTG Cuts at: 3449 3449 Size: 6427 SacII CC_GC'GG Cuts at: 2047 2047 Size: 6427 SalI G'TCGA_C Cuts at: 1936 3769 1936 Size: 1833 4594 SapI GCTCTTCn'nnn_ Cuts at: 2753 2753 Size: 6427 SbfI CC_TGCA'GG Cuts at: 3779 3779 Size: 6427 ScaI AGT'ACT Cuts at: 4675 6387 4675 Size: 1712 4715 SfcI C'TryA_G Cuts at: 826 1045 2235 2476 3775 4910 5591 5782 826 Size: 219 1190 241 1299 1135 681 191 1471 Fragments arranged by size: 1471 1299 1190 1135 681 241 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6427 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6427 SmaI CCC'GGG Cuts at: 1872 3754 1872 Size: 1882 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2411 4531 5432 5694 820 Size: 197 926 468 2120 901 262 1553 Fragments arranged by size: 2120 1553 926 901 468 262 197 SnaBI TAC'GTA Cuts at: 493 4218 493 Size: 3725 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6427 SphI G_CATG'C Cuts at: 1132 3785 1132 Size: 2653 3774 SspI AAT'ATT Cuts at: 5 52 4351 5 Size: 47 4299 2081 Fragments arranged by size: 4299 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6427 StyI C'CwwG_G Cuts at: 513 1065 2004 3353 3866 513 Size: 552 939 1349 513 3074 Fragments arranged by size: 3074 1349 939 552 513 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3300 4454 4471 4624 4809 5254 325 Size: 1255 1720 1154 17 153 185 445 1498 Fragments arranged by size: 1720 1498 1255 1154 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2328 2437 4673 Size: 274 80 33 51 1443 351 109 2236 Cuts at: 4673 6385 96 Size: 1712 138 Fragments arranged by size: 2236 1712 1443 351 274 138 109 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6387 6419 6387 Size: 32 6395 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6427 XbaI T'CTAG_A Cuts at: 3763 3763 Size: 6427 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6427 XmaI C'CCGG_G Cuts at: 1870 3752 1870 Size: 1882 4545 XmnI GAAnn'nnTTC Cuts at: 3179 4556 3179 Size: 1377 5050 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4235 276 Size: 53 83 186 936 2701 2468 Fragments arranged by size: 2701 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SanDI SexAI SfiI SfoI SrfI SwaI XcmI