################################## # NO CUT ENZYMES ( 90 enzymes) # ################################## AatII Acc65I AccII AclI AflII AflIII AgeI Alw44I ApaLI AscI AseI AsnI Asp700I Asp718I AspI BbrPI BclI BfrI BglI BglII BsaAI BsaBI BsiEI BsiWI BsmI BspDI BspHI BspLU11I BsrBI BssHII Bst1107I BstBI BstUI BstXI BstZ17I ClaI CpoI DraI EagI EclXI Eco52I EcoT22I FbaI FseI HincII HindII HindIII HpaI KpnI KspI MamI MfeI MluI MunI MvnI NdeI NheI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI ScaI SfiI SfuI SgrAI SnaBI SpeI SphI SspI StuI SwaI Tth111I XbaI XmnI ################################### # ONE CUT ENZYMES ( 65 enzymes) # ################################### AccI 1290 GTCTAC GT^[AC][GT]AC AccIII 108 TCCGGA T^CCGGA AcsI 619 GAATTC [GA]^AATT[CT] AfaI 324 GTAC GT^AC AhdI 1378 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 1331 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 1604 AGCGCT AGC^GCT ApaI 135 GGGCCC GGGCC^C ApoI 619 GAATTC [GA]^AATT[CT] AspEI 1378 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 1263 TGCGCA TGC^GCA AvrII 139 CCTAGG C^CTAGG BalI 1072 TGGCCA TGG^CCA BamHI 1173 GGATCC G^GATCC BbeI 822 GGCGCC GGCGC^C BcgI 1673 GCATCCTTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1938 GGATAC GTATCC(6/5) BfaI 140 CTAG C^TAG BlnI 139 CCTAGG C^CTAGG BseAI 108 TCCGGA T^CCGGA BsmBI 1353 CGTCTC CGTCTC(1/5) Bsp1407I 323 TGTACA T^GTACA BspEI 108 TCCGGA T^CCGGA BsrFI 271 GCCGGC [GA]^CCGG[CT] BsrGI 323 TGTACA T^GTACA BssSI 1681 CTCGTG CACGAG(-5/-1) BstAPI 1485 GCATCCCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 1173 GGATCC [GA]^GATC[CT] Cfr10I 271 GCCGGC [GA]^CCGG[CT] DrdI 366 GACAGAGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 538 CCATGG C^C[GA][CT]GG Eam1105I 1378 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1604 AGCGCT AGC^GCT EcoRI 619 GAATTC G^AATTC EcoRV 162 GATATC GAT^ATC FspI 1263 TGCGCA TGC^GCA KasI 822 GGCGCC G^GCGCC MaeI 140 CTAG C^TAG MflI 1173 GGATCC [GA]^GATC[CT] MluNI 1072 TGGCCA TGG^CCA MroI 108 TCCGGA T^CCGGA MscI 1072 TGGCCA TGG^CCA MseI 410 TTAA T^TAA NaeI 271 GCCGGC GCC^GGC NarI 822 GGCGCC GG^CGCC NcoI 538 CCATGG C^CATGG NgoMIV 271 GCCGGC G^CCGGC NspI 860 ACATGC [GA]CATG^[CT] PaeR7I 1894 CTCGAG C^TCGAG PflMI 1064 CCATGTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RsaI 324 GTAC GT^AC SbfI 1836 CCTGCAGG CCTGCA^GG SexAI 491 ACCAGGT A^CC[AT]GGT SfoI 822 GGCGCC GGC^GCC SmaI 1079 CCCGGG CCC^GGG SmlI 1894 CTCGAG C^T[CT][GA]AG Sse8387I 1836 CCTGCAGG CCTGCA^GG SspBI 323 TGTACA T^GTACA Tru9I 410 TTAA T^TAA Van91I 1064 CCATGTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 966 CCAATTTGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1894 CTCGAG C^TCGAG XhoII 1173 GGATCC [GA]^GATC[CT] XmaCI 1079 CCCGGG C^CCGGG XmaI 1079 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AspHI 183 GTGCTC G[AT]GC[AT]^C 1413 GAGCAC BanII 135 GGGCCC G[GA]GC[CT]^C 1082 GGGCTC BbsI 1462 GAAGAC GAAGAC(2/6) 1899 GAAGAC BlpI 275 GCTCAGC GC^T[ACGT]AGC 1410 GCTGAGC Bpu1102I 275 GCTCAGC GC^T[ACGT]AGC 1410 GCTGAGC BpuAI 1462 GAAGAC GAAGAC(2/6) 1899 GAAGAC BsaI 478 GAGACC GGTCTC(1/5) 1504 GAGACC BsaWI 108 TCCGGA [AT]^CCGG[AT] 521 ACCGGA BsiHKAI 183 GTGCTC G[AT]GC[AT]^C 1413 GAGCAC BsrDI 1219 GCAATG GCAATG(2/0) 1322 GCAATG BstEII 12 GGTGACC G^GT[ACGT]ACC 384 GGTGACC BstPI 12 GGTGACC G^GT[ACGT]ACC 384 GGTGACC CelII 275 GCTCAGC GC^T[ACGT]AGC 1410 GCTGAGC EaeI 452 TGGCCG [CT]^GGCC[GA] 1072 TGGCCA EcoNI 350 CCTGGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1836 CCTGCAGGAGG EcoO65I 12 GGTGACC G^GT[ACGT]ACC 384 GGTGACC MslI 435 CATCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1614 CATCGAAGTG Tsp509I 620 AATT ^AATT 968 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 85 enzymes) # ######################################### AciI AcyI AluI AlwI AocI AvaI AvaII BanI BbvCI BbvI BcnI BmyI BpmI BsaHI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DraIII EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI TseI Tsp45I TspRI TthHB8I