(Circular) (Six-base) MAPSORT of: pFN21AE2044.seq Check: 8103 from: 1 to: 6691 pFN21AE2044 6691 bp With 182 enzymes: SgfI * July 6, 2012 17:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3158 3550 3158 Size: 392 6299 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4501 278 Size: 53 83 186 936 2965 2468 Fragments arranged by size: 2965 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3296 4034 1937 Size: 1359 738 4594 Fragments arranged by size: 4594 1359 738 Acc65I G'GTAC_C Cuts at: 4012 4012 Size: 6691 AclI AA'CG_TT Cuts at: 1563 4818 5191 1563 Size: 3255 373 3063 Fragments arranged by size: 3255 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3598 4754 6138 6406 1632 Size: 1966 1156 1384 268 1917 Fragments arranged by size: 1966 1917 1384 1156 268 AfeI AGC'GCT Cuts at: 3611 3611 Size: 6691 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6494 AflIII A'CryG_T Cuts at: 1203 5590 6567 1203 Size: 4387 977 1327 Fragments arranged by size: 4387 1327 977 AgeI A'CCGG_T Cuts at: 5522 5522 Size: 6691 AhdI GACnn_n'nnGTC Cuts at: 3388 5420 3388 Size: 2032 4659 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6691 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2085 3024 3641 1184 Size: 901 939 617 4234 Fragments arranged by size: 4234 939 901 617 AlwNI CAG_nnn'CTG Cuts at: 3341 4434 6006 3341 Size: 1093 1572 4026 Fragments arranged by size: 4026 1572 1093 ApaI G_GGCC'C Cuts at: 2144 2144 Size: 6691 ApaLI G'TGCA_C Cuts at: 4748 5904 4748 Size: 1156 5535 ApoI r'AATT_y Cuts at: 1493 2624 4000 4226 4378 6487 1493 Size: 1131 1376 226 152 2109 1697 Fragments arranged by size: 2109 1697 1376 1131 226 152 AseI AT'TA_AT Cuts at: 160 1032 5245 160 Size: 872 4213 1606 Fragments arranged by size: 4213 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6691 AvaI C'yCGr_G Cuts at: 1870 1943 2137 2932 3084 3275 3899 4016 1870 Size: 73 194 795 152 191 624 117 4545 Fragments arranged by size: 4545 795 624 194 191 152 117 73 AvrII C'CTAG_G Cuts at: 2144 2144 Size: 6691 BamHI G'GATC_C Cuts at: 3178 4021 4388 3178 Size: 843 367 5481 Fragments arranged by size: 5481 843 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2523 2827 3403 3729 3923 Size: 325 217 627 736 304 576 326 194 Cuts at: 3923 4012 5467 618 Size: 89 1455 1842 Fragments arranged by size: 1842 1455 736 627 576 326 325 304 217 194 89 BanII G_rGCy'C Cuts at: 1391 2144 3091 4010 1391 Size: 753 947 919 4072 Fragments arranged by size: 4072 947 919 753 BbeI G_GCGC'C Cuts at: 2831 2831 Size: 6691 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3474 3911 928 Size: 971 1575 437 3708 Fragments arranged by size: 3708 1575 971 437 BbvCI CC'TCA_GC Cuts at: 2508 3334 3584 2508 Size: 826 250 5615 Fragments arranged by size: 5615 826 250 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3667 3701 4882 4916 1903 Size: 34 1730 34 1181 34 3678 Fragments arranged by size: 3678 1730 1181 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3937 4583 5793 3937 Size: 646 1210 4835 Fragments arranged by size: 4835 1210 646 BclI T'GATC_A Cuts at: 1548 1548 Size: 6691 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5302 136 Size: 107 122 71 631 605 3630 1525 Fragments arranged by size: 3630 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6686 6686 Size: 6691 BlpI GC'TnA_GC Cuts at: 2281 3416 4108 2281 Size: 1135 692 4864 Fragments arranged by size: 4864 1135 692 Bme1580I G_kGCm'C Cuts at: 1790 2144 3406 3740 3928 4752 5908 1790 Size: 354 1262 334 188 824 1156 2573 Fragments arranged by size: 2573 1262 1156 824 354 334 188 BmgBI CAC'GTC Cuts at: 1318 2997 3358 1318 Size: 1679 361 4651 Fragments arranged by size: 4651 1679 361 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3409 3809 4422 Size: 416 53 83 399 24 1992 400 613 Cuts at: 4422 5380 442 Size: 958 1753 Fragments arranged by size: 1992 1753 958 613 416 400 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6691 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2624 2689 2819 3226 3616 3804 5351 1239 Size: 1385 65 130 407 390 188 1547 2579 Fragments arranged by size: 2579 1547 1385 407 390 188 130 65 Bpu10I CC'TnA_GC Cuts at: 2246 2508 3334 3584 3674 3930 2246 Size: 262 826 250 90 256 5007 Fragments arranged by size: 5007 826 262 256 250 90 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4816 5681 5979 4816 Size: 865 298 5528 Fragments arranged by size: 5528 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2477 3503 5354 882 Size: 617 978 1026 1851 2219 Fragments arranged by size: 2219 1851 1026 978 617 BsaAI yAC'GTr Cuts at: 493 1204 4482 493 Size: 711 3278 2702 Fragments arranged by size: 3278 2702 711 BsaBI GATnn'nnATC Cuts at: 6685 6685 Size: 6691 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2828 3432 3693 4498 Size: 53 83 186 936 1295 604 261 805 Cuts at: 4498 4880 5515 275 Size: 382 635 1451 Fragments arranged by size: 1451 1295 936 805 635 604 382 261 186 83 53 BsaWI w'CCGG_w Cuts at: 2113 2526 5124 5522 5796 5943 6354 2113 Size: 413 2598 398 274 147 411 2450 Fragments arranged by size: 2598 2450 413 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2174 2204 2844 2874 1178 Size: 30 165 30 771 30 640 30 4995 Fragments arranged by size: 4995 771 640 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2027 2418 2776 2953 1186 Size: 841 391 358 177 4924 Fragments arranged by size: 4924 841 391 358 177 BseYI C'CCAG_C Cuts at: 2216 2312 2534 2647 2746 2793 3341 3606 5894 Size: 96 222 113 99 47 548 265 2288 Cuts at: 5894 6390 2216 Size: 496 2517 Fragments arranged by size: 2517 2288 548 496 265 222 113 99 96 47 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2534 2891 3518 3578 3647 1163 Size: 573 798 357 627 60 69 4207 Fragments arranged by size: 4207 798 627 573 357 69 60 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4141 4902 5930 1540 Size: 275 186 2140 761 1028 2301 Fragments arranged by size: 2301 2140 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2192 3422 4752 4837 5908 2192 Size: 1230 1330 85 1071 2975 Fragments arranged by size: 2975 1330 1230 1071 85 BsmI GAATG_Cn' Cuts at: 4202 4295 4202 Size: 93 6598 BsmBI CGTCTCn'nnnn_ Cuts at: 3364 3364 Size: 6691 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2144 2192 3091 3406 3422 3740 3928 Size: 399 354 48 899 315 16 318 188 Cuts at: 3928 4010 4752 4837 5908 1391 Size: 82 742 85 1071 2174 Fragments arranged by size: 2174 1071 899 742 399 354 318 315 188 85 82 48 16 BspEI T'CCGG_A Cuts at: 2113 6354 2113 Size: 4241 2450 BspHI T'CATG_A Cuts at: 4580 6310 4580 Size: 1730 4961 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3158 3550 3619 4046 844 Size: 2314 392 69 427 3489 Fragments arranged by size: 3489 2314 427 392 69 BsrBI CCG'CTC Cuts at: 1394 1823 4578 1394 Size: 429 2755 3507 Fragments arranged by size: 3507 2755 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3231 3334 5186 5360 65 Size: 1183 1983 103 1852 174 1396 Fragments arranged by size: 1983 1852 1396 1183 174 103 BsrFI r'CCGG_y Cuts at: 1700 1810 2276 5335 5522 1700 Size: 110 466 3059 187 2869 Fragments arranged by size: 3059 2869 466 187 110 BsrGI T'GTAC_A Cuts at: 96 2328 96 Size: 2232 4459 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6691 BssSI C'ACGA_G Cuts at: 3686 4751 5763 3686 Size: 1065 1012 4614 Fragments arranged by size: 4614 1065 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3496 794 Size: 2702 3989 BstBI TT'CG_AA Cuts at: 5495 5495 Size: 6691 BstEII G'GTnAC_C Cuts at: 1184 2017 2389 1184 Size: 833 372 5486 Fragments arranged by size: 5486 833 372 BstYI r'GATC_y Cuts at: 1972 3178 4021 4388 4773 4790 6242 6328 6340 Size: 1206 843 367 385 17 1452 86 12 Cuts at: 6340 6686 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1206 843 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2385 2568 2721 3918 2385 Size: 183 153 1197 5158 Fragments arranged by size: 5158 1197 183 153 BtgI C'CryG_G Cuts at: 513 1065 2004 2543 513 Size: 552 939 539 4661 Fragments arranged by size: 4661 939 552 539 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5952 Fragments arranged by size: 5952 683 56 BtsI GCAGTG_nn' Cuts at: 3809 4207 5001 5021 3809 Size: 398 794 20 5479 Fragments arranged by size: 5479 794 398 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6691 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6656 DraI TTT'AAA Cuts at: 3995 3995 Size: 6691 DraIII CAC_nnn'GTG Cuts at: 3291 3579 3758 3291 Size: 288 179 6224 Fragments arranged by size: 6224 288 179 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2377 5698 809 Size: 507 1061 3321 1802 Fragments arranged by size: 3321 1802 1061 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2457 3077 4138 Size: 54 1425 50 275 18 627 620 1061 Cuts at: 4138 5027 6656 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1061 889 627 620 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4138 1537 Size: 275 2326 4090 Fragments arranged by size: 4090 2326 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2045 2051 3825 4621 1353 Size: 692 6 1774 796 3423 Fragments arranged by size: 3423 1774 796 692 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3757 5278 5652 5798 387 Size: 1064 138 2168 1521 374 146 1280 Fragments arranged by size: 2168 1521 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2624 2689 2819 3226 3598 3616 3804 Size: 393 992 65 130 407 372 18 188 Cuts at: 3804 4754 5351 6138 6406 1239 Size: 950 597 787 268 1524 Fragments arranged by size: 1524 992 950 787 597 407 393 372 268 188 130 65 18 EcoNI CCTnn'n_nnAGG Cuts at: 2359 3845 2359 Size: 1486 5205 EcoO109I rG'GnC_Cy Cuts at: 2101 2141 2149 2207 2901 3139 3850 2101 Size: 40 8 58 694 238 711 4942 Fragments arranged by size: 4942 711 694 238 58 40 8 EcoRI G'AATT_C Cuts at: 2624 4000 4378 2624 Size: 1376 378 4937 Fragments arranged by size: 4937 1376 378 EcoRV GAT'ATC Cuts at: 2169 2169 Size: 6691 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3304 3304 Size: 6691 FspI TGC'GCA Cuts at: 3270 5197 3270 Size: 1927 4764 HaeII r_GCGC'y Cuts at: 1678 2070 2260 2831 3613 5838 1678 Size: 392 190 571 782 2225 2531 Fragments arranged by size: 2531 2225 782 571 392 190 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2108 2114 2140 2146 2178 Size: 32 135 32 536 6 26 6 32 Cuts at: 2178 2570 2602 2844 2876 2911 2943 3374 3406 Size: 392 32 242 32 35 32 431 32 Cuts at: 3406 3409 3441 3880 3912 5332 5364 5406 5438 Size: 3 32 439 32 1420 32 42 32 Cuts at: 5438 1373 Size: 2626 Fragments arranged by size: 2626 1420 536 439 431 392 242 135 42 35 32 32 32 32 32 32 32 32 32 32 32 26 6 6 3 HincII GTy'rAC Cuts at: 669 1657 1938 4035 4281 669 Size: 988 281 2097 246 3079 Fragments arranged by size: 3079 2097 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6691 HpaI GTT'AAC Cuts at: 4281 4281 Size: 6691 KasI G'GCGC_C Cuts at: 2827 2827 Size: 6691 KpnI G_GTAC'C Cuts at: 4016 4016 Size: 6691 MfeI C'AATT_G Cuts at: 4290 4290 Size: 6691 MluI A'CGCG_T Cuts at: 6567 6567 Size: 6691 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2328 2659 3031 5805 5989 1305 Size: 1023 331 372 2774 184 2007 Fragments arranged by size: 2774 2007 1023 372 331 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3079 10 Size: 54 1425 343 1247 3622 Fragments arranged by size: 3622 1425 1247 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2444 3623 4649 5008 5167 518 Size: 648 1278 1179 1026 359 159 2042 Fragments arranged by size: 2042 1278 1179 1026 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2014 2216 2467 2647 2746 2797 3204 3341 Size: 408 202 251 180 99 51 407 137 Cuts at: 3341 3456 3745 4107 4784 5932 6177 1606 Size: 115 289 362 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 408 407 362 289 251 245 202 180 137 115 99 51 NaeI GCC'GGC Cuts at: 2278 2278 Size: 6691 NarI GG'CG_CC Cuts at: 2828 2828 Size: 6691 NcoI C'CATG_G Cuts at: 513 1065 2004 2543 513 Size: 552 939 539 4661 Fragments arranged by size: 4661 939 552 539 NdeI CA'TA_TG Cuts at: 387 387 Size: 6691 NgoMIV G'CCGG_C Cuts at: 2276 2276 Size: 6691 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6691 NotI GC'GGCC_GC Cuts at: 4138 4138 Size: 6691 NspI r_CATG'y Cuts at: 1132 1229 2869 4049 5594 1132 Size: 97 1640 1180 1545 2229 Fragments arranged by size: 2229 1640 1545 1180 97 PasI CC'CwG_GG Cuts at: 1348 3661 1348 Size: 2313 4378 PciI A'CATG_T Cuts at: 5590 5590 Size: 6691 PflMI CCAn_nnn'nTGG Cuts at: 1108 3075 1108 Size: 1967 4724 PfoI T'CCnGG_A Cuts at: 2666 3825 2666 Size: 1159 5532 PmeI GTTT'AAAC Cuts at: 3995 3995 Size: 6691 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2501 2533 4760 4792 6273 6305 2501 Size: 32 2227 32 1481 32 2887 Fragments arranged by size: 2887 2227 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2101 2149 2207 2901 2101 Size: 48 58 694 5891 Fragments arranged by size: 5891 694 58 48 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6691 PsiI TTA'TAA Cuts at: 4261 4261 Size: 6691 PspOMI G'GGCC_C Cuts at: 2140 2140 Size: 6691 PstI C_TGCA'G Cuts at: 830 2078 2298 2400 3155 3846 4043 830 Size: 1248 220 102 755 691 197 3478 Fragments arranged by size: 3478 1248 755 691 220 197 102 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6691 PvuII CAG'CTG Cuts at: 2216 2467 2647 2746 2797 3204 3341 2216 Size: 251 180 99 51 407 137 5566 Fragments arranged by size: 5566 407 251 180 137 99 51 SalI G'TCGA_C Cuts at: 1936 4033 1936 Size: 2097 4594 SanDI GG'GwC_CC Cuts at: 2207 2207 Size: 6691 SbfI CC_TGCA'GG Cuts at: 3846 4043 3846 Size: 197 6494 ScaI AGT'ACT Cuts at: 4939 6651 4939 Size: 1712 4979 SexAI A'CCwGG_T Cuts at: 2496 2496 Size: 6691 SfcI C'TryA_G Cuts at: 826 1045 2074 2294 2396 2855 3151 3199 3842 Size: 219 1029 220 102 459 296 48 643 Cuts at: 3842 4039 5174 5855 6046 826 Size: 197 1135 681 191 1471 Fragments arranged by size: 1471 1135 1029 681 643 459 296 220 219 197 191 102 48 SfoI GGC'GCC Cuts at: 2829 2829 Size: 6691 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6691 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6691 SmaI CCC'GGG Cuts at: 1872 3086 4018 1872 Size: 1214 932 4545 Fragments arranged by size: 4545 1214 932 SmlI C'TyrA_G Cuts at: 820 1017 1943 3899 4795 5696 5958 820 Size: 197 926 1956 896 901 262 1553 Fragments arranged by size: 1956 1553 926 901 896 262 197 SnaBI TAC'GTA Cuts at: 493 4482 493 Size: 3989 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6691 SphI G_CATG'C Cuts at: 1132 4049 1132 Size: 2917 3774 SrfI GCCC'GGGC Cuts at: 3086 3086 Size: 6691 SspI AAT'ATT Cuts at: 5 52 4615 5 Size: 47 4563 2081 Fragments arranged by size: 4563 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6691 StyI C'CwwG_G Cuts at: 513 1065 2004 2144 2543 2656 2889 3697 3706 Size: 552 939 140 399 113 233 808 9 Cuts at: 3706 3957 4130 513 Size: 251 173 3074 Fragments arranged by size: 3074 939 808 552 399 251 233 173 140 113 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2014 2335 2548 3032 4718 4735 4888 Size: 1255 434 321 213 484 1686 17 153 Cuts at: 4888 5073 5518 325 Size: 185 445 1498 Fragments arranged by size: 1686 1498 1255 484 445 434 321 213 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2328 4937 6649 Size: 274 80 33 51 1443 351 2609 1712 Cuts at: 6649 96 Size: 138 Fragments arranged by size: 2609 1712 1443 351 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2158 2190 2855 2887 6651 6683 2158 Size: 32 665 32 3764 32 2166 Fragments arranged by size: 3764 2166 665 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6691 XbaI T'CTAG_A Cuts at: 4027 4027 Size: 6691 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2978 2011 Size: 967 5724 XhoI C'TCGA_G Cuts at: 1943 3899 1943 Size: 1956 4735 XmaI C'CCGG_G Cuts at: 1870 3084 4016 1870 Size: 1214 932 4545 Fragments arranged by size: 4545 1214 932 XmnI GAAnn'nnTTC Cuts at: 4820 4820 Size: 6691 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4499 276 Size: 53 83 186 936 2965 2468 Fragments arranged by size: 2965 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BaeI BfrBI BplI BsiWI BstXI BstZ17I EcoICRI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SapI SfiI SwaI