Restriction map for pFN21AB5986
 (Linear) (Six-base) MAPSORT of: KIBB5986.seq  Check: 5087  from: 1  to: 1707



KIBB5986.orf

 With 182 enzymes: SgfI * 

                        February 8, 2013 18:16  ..

AccI GT'mk_AC

Cuts at:      0     713    1195    1707 
   Size:        713     482     512

  Fragments arranged by size: 

                 713     512     482
 
Acc65I G'GTAC_C

Cuts at:      0    1604    1707 
   Size:       1604     103
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1121    1365    1424    1707 
   Size:       1121     244      59     283

  Fragments arranged by size: 

                1121     283     244      59
 
AleI CACnn'nnGTG

Cuts at:      0     521    1707 
   Size:        521    1186
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1653    1707 
   Size:       1653      54
 
ApoI r'AATT_y

Cuts at:      0     297     334     640    1510    1707 
   Size:        297      37     306     870     197

  Fragments arranged by size: 

                 870     306     297     197      37
 
AseI AT'TA_AT

Cuts at:      0    1319    1707 
   Size:       1319     388
 
AvaI C'yCGr_G

Cuts at:      0     629    1702    1707 
   Size:        629    1073       5

  Fragments arranged by size: 

                1073     629       5
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     179     212    1707 
   Size:        179      33    1495

  Fragments arranged by size: 

                1495     179      33
 
BamHI G'GATC_C

Cuts at:      0     721    1707 
   Size:        721     986
 
BanI G'GyrC_C

Cuts at:      0    1604    1707 
   Size:       1604     103
 
BanII G_rGCy'C

Cuts at:      0     691    1707 
   Size:        691    1016
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     833    1307    1350    1707 
   Size:        833     474      43     357

  Fragments arranged by size: 

                 833     474     357      43
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1181    1707 
   Size:       1181     526
 
BfrBI ATG'CAT

Cuts at:      0     592    1707 
   Size:        592    1115
 
Bme1580I G_kGCm'C

Cuts at:      0     427    1707 
   Size:        427    1280
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     808    1707 
   Size:        808     899
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73     198    1489    1707 
   Size:         73     125    1291     218

  Fragments arranged by size: 

                1291     218     125      73
 
Bpu10I CC'TnA_GC

Cuts at:      0     257    1707 
   Size:        257    1450
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1483    1707 
   Size:       1483     224
 
BsaAI yAC'GTr

Cuts at:      0    1695    1707 
   Size:       1695      12
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     404     434    1707 
   Size:        404      30    1273

  Fragments arranged by size: 

                1273     404      30
 
BseYI C'CCAG_C

Cuts at:      0     690    1389    1707 
   Size:        690     699     318

  Fragments arranged by size: 

                 699     690     318
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     401    1707 
   Size:        401    1306
 
Bsp1286I G_dGCh'C

Cuts at:      0     427     691    1707 
   Size:        427     264    1016

  Fragments arranged by size: 

                1016     427     264
 
BsrDI GCAATG_nn'

Cuts at:      0     767    1707 
   Size:        767     940
 
BsrFI r'CCGG_y

Cuts at:      0     751    1707 
   Size:        751     956
 
BstEII G'GTnAC_C

Cuts at:      0    1628    1707 
   Size:       1628      79
 
BstYI r'GATC_y

Cuts at:      0     721    1707 
   Size:        721     986
 
Bsu36I CC'TnA_GG

Cuts at:      0    1235    1707 
   Size:       1235     472
 
ClaI AT'CG_AT

Cuts at:      0    1504    1707 
   Size:       1504     203
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     320     355    1707 
   Size:        320      35    1352

  Fragments arranged by size: 

                1352     320      35
 
DraI TTT'AAA

Cuts at:      0     444    1224    1707 
   Size:        444     780     483

  Fragments arranged by size: 

                 780     483     444
 
EaeI y'GGCC_r

Cuts at:      0     753    1707 
   Size:        753     954
 
EarI CTCTTCn'nnn_

Cuts at:      0    1400    1707 
   Size:       1400     307
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73     198    1121    1365    1424    1489    1707 
   Size:         73     125     923     244      59      65     218

  Fragments arranged by size: 

                 923     244     218     125      73      65      59
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     256    1707 
   Size:        256    1451
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      33      65     706     738    1337    1369    1707 
   Size:         33      32     641      32     599      32     338

  Fragments arranged by size: 

                 641     599     338      33      32      32      32
 
HincII GTy'rAC

Cuts at:      0    1034    1707 
   Size:       1034     673
 
HindIII A'AGCT_T

Cuts at:      0    1133    1707 
   Size:       1133     574
 
KpnI G_GTAC'C

Cuts at:      0    1608    1707 
   Size:       1608      99
 
MfeI C'AATT_G

Cuts at:      0     733    1707 
   Size:        733     974
 
MslI CAynn'nnrTG

Cuts at:      0     521     547     880    1045    1707 
   Size:        521      26     333     165     662

  Fragments arranged by size: 

                 662     521     333     165      26
 
MspA1I CmG'CkG

Cuts at:      0    1385    1541    1707 
   Size:       1385     156     166

  Fragments arranged by size: 

                1385     166     156
 
NsiI A_TGCA'T

Cuts at:      0     594    1707 
   Size:        594    1113
 
NspI r_CATG'y

Cuts at:      0    1614    1707 
   Size:       1614      93
 
PfoI T'CCnGG_A

Cuts at:      0     214    1707 
   Size:        214    1493
 
PmlI CAC'GTG

Cuts at:      0    1695    1707 
   Size:       1695      12
 
PshAI GACnn'nnGTC

Cuts at:      0    1569    1707 
   Size:       1569     138
 
PsiI TTA'TAA

Cuts at:      0     365     917    1707 
   Size:        365     552     790

  Fragments arranged by size: 

                 790     552     365
 
PvuII CAG'CTG

Cuts at:      0    1385    1707 
   Size:       1385     322
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1400    1707 
   Size:       1400     307
 
SfcI C'TryA_G

Cuts at:      0     200    1707 
   Size:        200    1507
 
SmaI CCC'GGG

Cuts at:      0     631    1707 
   Size:        631    1076
 
SmlI C'TyrA_G

Cuts at:      0    1462    1707 
   Size:       1462     245
 
StuI AGG'CCT

Cuts at:      0     675    1707 
   Size:        675    1032
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1381    1707 
   Size:       1381     326
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     404     436    1237    1269    1707 
   Size:        404      32     801      32     438

  Fragments arranged by size: 

                 801     438     404      32      32
 
XbaI T'CTAG_A

Cuts at:      0     651    1707 
   Size:        651    1056
 
XmaI C'CCGG_G

Cuts at:      0     629    1707 
   Size:        629    1078
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI     AleI    AlwNI     ApoI     AseI     AvaI
     BaeI    BamHI     BanI    BanII     BbsI    BciVI    BfrBI Bme1580I
     BmrI     BpmI   Bpu10I    BpuEI    BsaAI    BsaXI    BseYI    BsmBI
 Bsp1286I    BsrDI    BsrFI   BstEII    BstYI   Bsu36I     ClaI    CspCI
     DraI     EaeI     EarI  Eco57MI     FalI    Hin4I   HincII  HindIII
     KpnI     MfeI     MslI   MspA1I     NsiI     NspI     PfoI     PmlI
    PshAI     PsiI    PvuII     SapI     SfcI     SmaI     SmlI     StuI
    TaqII     TstI     XbaI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI    AvrII     BbeI
    BbvCI     BcgI     BclI     BglI    BglII     BlpI    BmgBI     BmtI
     BplI     BsaI    BsaBI    BsaHI    BsaWI    BseRI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BspEI    BspHI    BspMI    BsrBI    BsrGI
   BssHII    BssSI   BstAPI    BstBI    BstXI  BstZ17I     BtgI    BtgZI
     BtsI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII     HpaI     KasI
     MluI     MmeI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     PacI     PasI     PciI    PflMI     PmeI
     PpiI    PpuMI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI
     StyI     SwaI     TatI  Tth111I     XcmI     XhoI     XmnI     ZraI
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