################################## # NO CUT ENZYMES ( 162 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AcyI AflII AgeI AhdI Alw44I AocI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbsI BbvI BcgI BcnI BfrI BglI BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsmFI BsmI BsoBI BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII Bst1107I BstAPI BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT14I EcoT22I FauI Fnu4HI FseI FspI HapII HgaI Hin1I HincII HindII HindIII HpaI HpaII ItaI KasI KpnI KspI MaeII MamI MluI MroI MslI MspA1I MspI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI StyI SwaI TseI Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccI 689 GTAGAC GT^[AC][GT]AC AcsI 544 GAATTC [GA]^AATT[CT] AflIII 873 ACATGT A^C[GA][CT]GT AlwI 552 GATCC GGATC(4/5) Aor51HI 1088 AGCGCT AGC^GCT ApoI 544 GAATTC [GA]^AATT[CT] AvaII 1000 GGTCC G^G[AT]CC BanI 626 GGTGCC G^G[CT][GA]CC BanII 947 GGGCTC G[GA]GC[CT]^C BbvCI 989 CCTCAGC CCTCAGC(-5/-2) BciVI 1071 GTATCC GTATCC(6/5) BclI 612 TGATCA T^GATCA BglII 706 AGATCT A^GATCT BmyI 947 GGGCTC G[AGT]GC[ACT]^C BseRI 759 GAGGAG GAGGAG(10/8) Bsp1286I 947 GGGCTC G[AGT]GC[ACT]^C BspDI 332 ATCGAT AT^CGAT BspLU11I 873 ACATGT A^CATGT BssSI 41 CTCGTG CACGAG(-5/-1) BstBI 232 TTCGAA TT^CGAA BstXI 905 CCAAGTCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 706 AGATCT [GA]^GATC[CT] Cac8I 423 GCTGGC GC[ACGT]^[ACGT]GC CfoI 1089 GCGC GCG^C ClaI 332 ATCGAT AT^CGAT DraII 999 AGGTCCT [GA]G^G[ACGT]CC[CT] Eco47III 1088 AGCGCT AGC^GCT EcoO109I 999 AGGTCCT [GA]G^G[ACGT]CC[CT] EcoRI 544 GAATTC G^AATTC FbaI 612 TGATCA T^GATCA HaeII 1088 AGCGCT [GA]GCGC^[CT] HhaI 1089 GCGC GCG^C HinP1I 1089 GCGC G^CGC HphI 861 TCACC GGTGA(8/7) MflI 706 AGATCT [GA]^GATC[CT] MseI 164 TTAA T^TAA NspI 873 ACATGT [GA]CATG^[CT] NspV 232 TTCGAA TT^CGAA PleI 463 GAGTC GAGTC(4/5) PpuMI 999 AGGTCCT [GA]G^G[AT]CC[CT] PshAI 648 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SapI 856 GCTCTTC GCTCTTC(1/4) SfcI 851 CTGTAG C^T[GA][CT]AG SfuI 232 TTCGAA TT^CGAA SmlI 786 CTCAAG C^T[CT][GA]AG Tru9I 164 TTAA T^TAA XcmI 630 CCAAAGCCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 706 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AlwNI 296 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG 845 CAGATTCTG BalI 170 TGGCCA TGG^CCA 914 TGGCCA BfaI 91 CTAG C^TAG 454 CTAG Bsp1407I 212 TGTACA T^GTACA 292 TGTACA BsrGI 212 TGTACA T^GTACA 292 TGTACA Cfr13I 442 GGGCC G^G[ACGT]CC 1000 GGTCC EarI 657 CTCTTC CTCTTC(1/4) 857 CTCTTC Ksp632I 657 CTCTTC CTCTTC(1/4) 857 CTCTTC MaeI 91 CTAG C^TAG 454 CTAG MfeI 812 CAATTG C^AATTG 968 CAATTG MluNI 170 TGGCCA TGG^CCA 914 TGGCCA MscI 170 TGGCCA TGG^CCA 914 TGGCCA MunI 812 CAATTG C^AATTG 968 CAATTG Sau96I 442 GGGCC G^G[ACGT]CC 1000 GGTCC SspBI 212 TGTACA T^GTACA 292 TGTACA SspI 586 AATATT AAT^ATT 1113 AATATT TfiI 450 GATTC G^A[AT]TC 847 GATTC Tsp45I 909 GTCAC ^GT[GC]AC 1082 GTCAC TspRI 911 CACTG CA[GC]TG[ACGT][ACGT]^ 1039 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 29 enzymes) # ######################################### AfaI AluI BsmAI BsrI BssKI BstF5I BstNI DdeI DpnII EaeI EcoRII FokI HaeIII HinfI MaeIII MboI MboII MnlI MvaI NdeII NlaIII NlaIV RsaI Sau3AI ScrFI SfaNI TaqI Tsp509I TthHB8I