Restriction map for pFN21AB6536
 (Circular) (Six-base) MAPSORT of: pFN21AB6536.seq  Check: 3307  from: 1  to: 5875



pFN21AB6536  5875 bp

 With 182 enzymes: SgfI * 

                        July 6, 2012 17:36  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3685     278 
   Size:         53      83     186     936    2149    2468

  Fragments arranged by size: 

                2468    2149     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2695    3218    1937 
   Size:        758     523    4594

  Fragments arranged by size: 

                4594     758     523
 
Acc65I G'GTAC_C

Cuts at:   3196    3196 
   Size:       5875
 
AclI AA'CG_TT

Cuts at:   1563    4002    4375    1563 
   Size:       2439     373    3063

  Fragments arranged by size: 

                3063    2439     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3026    3938    5322    5590    1632 
   Size:       1394     912    1384     268    1917

  Fragments arranged by size: 

                1917    1394    1384     912     268
 
AfeI AGC'GCT

Cuts at:   3095    3095 
   Size:       5875
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5678
 
AflIII A'CryG_T

Cuts at:   1203    2878    4774    5751    1203 
   Size:       1675    1896     977    1327

  Fragments arranged by size: 

                1896    1675    1327     977
 
AgeI A'CCGG_T

Cuts at:   4706    4706 
   Size:       5875
 
AhdI GACnn_n'nnGTC

Cuts at:   4604    4604 
   Size:       5875
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5875
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5875
 
AlwNI CAG_nnn'CTG

Cuts at:   2306    2855    3618    5190    2306 
   Size:        549     763    1572    2991

  Fragments arranged by size: 

                2991    1572     763     549
 
ApaLI G'TGCA_C

Cuts at:   3932    5088    3932 
   Size:       1156    4719
 
ApoI r'AATT_y

Cuts at:   1493    2549    3184    3410    3562    5671    1493 
   Size:       1056     635     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1056     635     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4429     160 
   Size:        872    3397    1606

  Fragments arranged by size: 

                3397    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5875
 
AvaI C'yCGr_G

Cuts at:   1870    1943    3200    1870 
   Size:         73    1257    4545

  Fragments arranged by size: 

                4545    1257      73
 
BamHI G'GATC_C

Cuts at:   3205    3572    3205 
   Size:        367    5508
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2631    3196    4651     618 
   Size:        325     217     627     844     565    1455    1842

  Fragments arranged by size: 

                1842    1455     844     627     565     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2956    3194    1391 
   Size:       1565     238    4072

  Fragments arranged by size: 

                4072    1565     238
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4904
 
BbvCI CC'TCA_GC

Cuts at:   2995    2995 
   Size:       5875
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    1987    2021    4066    4100    1903 
   Size:         34      50      34    2045      34    3678

  Fragments arranged by size: 

                3678    2045      50      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3087    3767    4977    3087 
   Size:        680    1210    3985

  Fragments arranged by size: 

                3985    1210     680
 
BclI T'GATC_A

Cuts at:   1548    2617    1548 
   Size:       1069    4806
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4486     136 
   Size:        107     122      71     631     605    2814    1525

  Fragments arranged by size: 

                2814    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   2711    5870    2711 
   Size:       3159    2716
 
BlpI GC'TnA_GC

Cuts at:   3292    3292 
   Size:       5875
 
Bme1580I G_kGCm'C

Cuts at:   1790    3936    5092    1790 
   Size:       2146    1156    2573

  Fragments arranged by size: 

                2573    2146    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5875
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2394    2555    3606 
   Size:        416      53      83     399      24     977     161    1051

Cuts at:   3606    4564     442 
   Size:        958    1753

  Fragments arranged by size: 

                1753    1051     977     958     416     399     161      83
                  53      24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5875
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    4535    1239 
   Size:       3296    2579
 
Bpu10I CC'TnA_GC

Cuts at:   2995    2995 
   Size:       5875
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2776    4000    4865    5163    2776 
   Size:       1224     865     298    3488

  Fragments arranged by size: 

                3488    1224     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4538     882 
   Size:        617    3039    2219

  Fragments arranged by size: 

                3039    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3666     493 
   Size:        711    2462    2702

  Fragments arranged by size: 

                2702    2462     711
 
BsaBI GATnn'nnATC

Cuts at:   5869    5869 
   Size:       5875
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3682    4064    4699     275 
   Size:         53      83     186     936    2149     382     635    1451

  Fragments arranged by size: 

                2149    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   4308    4706    4980    5127    5538    4308 
   Size:        398     274     147     411    4645

  Fragments arranged by size: 

                4645     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2758    2788    1178 
   Size:         30     165      30    1355      30    4265

  Fragments arranged by size: 

                4265    1355     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2779    1186 
   Size:       1593    4282
 
BseYI C'CCAG_C

Cuts at:   2949    5078    5574    2949 
   Size:       2129     496    3250

  Fragments arranged by size: 

                3250    2129     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    5302
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3325    4086    5114    1540 
   Size:        275     186    1324     761    1028    2301

  Fragments arranged by size: 

                2301    1324    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3936    4021    5092    3936 
   Size:         85    1071    4719

  Fragments arranged by size: 

                4719    1071      85
 
BsmI GAATG_Cn'

Cuts at:   3386    3479    3386 
   Size:         93    5782
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2956    3194    3936    4021    5092    1391 
   Size:        399    1166     238     742      85    1071    2174

  Fragments arranged by size: 

                2174    1166    1071     742     399     238      85
 
BspEI T'CCGG_A

Cuts at:   5538    5538 
   Size:       5875
 
BspHI T'CATG_A

Cuts at:   3764    5494    3764 
   Size:       1730    4145
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3230     844 
   Size:       2386    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3762    1394 
   Size:        429    1939    3507

  Fragments arranged by size: 

                3507    1939     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4370    4544      65 
   Size:       1183    3122     174    1396

  Fragments arranged by size: 

                3122    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4519    4706    1700 
   Size:        110    2709     187    2869

  Fragments arranged by size: 

                2869    2709     187     110
 
BsrGI T'GTAC_A

Cuts at:     96    2217    2297      96 
   Size:       2121      80    3674

  Fragments arranged by size: 

                3674    2121      80
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5875
 
BssSI C'ACGA_G

Cuts at:   2046    3935    4947    2046 
   Size:       1889    1012    2974

  Fragments arranged by size: 

                2974    1889    1012
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5875
 
BstBI TT'CG_AA

Cuts at:   2238    4679    2238 
   Size:       2441    3434
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5875
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2917    2917 
   Size:       5875
 
BstYI r'GATC_y

Cuts at:   1972    2711    3205    3572    3957    3974    5426    5512    5524 
   Size:        739     494     367     385      17    1452      86      12

Cuts at:   5524    5870    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     739     494     385     367     346      86
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065     513 
   Size:        552    5323
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5136

  Fragments arranged by size: 

                5136     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3391    4185    4205    3391 
   Size:        794      20    5061

  Fragments arranged by size: 

                5061     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    2338    1554 
   Size:        784    5091
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564    2656    2691     529 
   Size:         35    2092      35    3713

  Fragments arranged by size: 

                3713    2092      35      35
 
DraI TTT'AAA

Cuts at:   3179    3179 
   Size:       5875
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4882     809 
   Size:        507    3566    1802

  Fragments arranged by size: 

                3566    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2175    2415    2919 
   Size:         54    1425      50     275      18     345     240     504

Cuts at:   2919    3322    4211    5840       8 
   Size:        403     889    1629      43

  Fragments arranged by size: 

                1629    1425     889     504     403     345     275     240
                  54      50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3322    1537 
   Size:        275    1510    4090

  Fragments arranged by size: 

                4090    1510     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2668    2868    3805    1353 
   Size:       1315     200     937    3423

  Fragments arranged by size: 

                3423    1315     937     200
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4462    4836    4982     387 
   Size:       1064     138    2873     374     146    1280

  Fragments arranged by size: 

                2873    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    3026    3938    4535    5322    5590    1239 
   Size:        393    1394     912     597     787     268    1524

  Fragments arranged by size: 

                1524    1394     912     787     597     393     268
 
EcoO109I rG'GnC_Cy

Cuts at:   3005    3005 
   Size:       5875
 
EcoRI G'AATT_C

Cuts at:   2549    3184    3562    2549 
   Size:        635     378    4862

  Fragments arranged by size: 

                4862     635     378
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   3127    3127 
   Size:       5875
 
FspI TGC'GCA

Cuts at:   4381    4381 
   Size:       5875
 
HaeII r_GCGC'y

Cuts at:   1678    3097    5022    1678 
   Size:       1419    1925    2531

  Fragments arranged by size: 

                2531    1925    1419
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2389    2421    4516    4548    4590 
   Size:         32     135      32     817      32    2095      32      42

Cuts at:   4590    4622    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    2095     817     135      42      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3219    3465     669 
   Size:        988     281    1281     246    3079

  Fragments arranged by size: 

                3079    1281     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5875
 
HpaI GTT'AAC

Cuts at:   3465    3465 
   Size:       5875
 
KpnI G_GTAC'C

Cuts at:   3200    3200 
   Size:       5875
 
MfeI C'AATT_G

Cuts at:   2817    2973    3474    2817 
   Size:        156     501    5218

  Fragments arranged by size: 

                5218     501     156
 
MluI A'CGCG_T

Cuts at:   5751    5751 
   Size:       5875
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2691    4989    5173    1305 
   Size:       1386    2298     184    2007

  Fragments arranged by size: 

                2298    2007    1386     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2177    2921      10 
   Size:         54    1425     343     345     744    2964

  Fragments arranged by size: 

                2964    1425     744     345     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3833    4192    4351     518 
   Size:        648    2667     359     159    2042

  Fragments arranged by size: 

                2667    2042     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    3291    3968    5116    5361    1606 
   Size:       1685     677    1148     245    2120

  Fragments arranged by size: 

                2120    1685    1148     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    5323
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5875
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5875
 
NotI GC'GGCC_GC

Cuts at:   3322    3322 
   Size:       5875
 
NspI r_CATG'y

Cuts at:   1132    1229    2882    3233    4778    1132 
   Size:         97    1653     351    1545    2229

  Fragments arranged by size: 

                2229    1653    1545     351      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5875
 
PciI A'CATG_T

Cuts at:   2878    4774    2878 
   Size:       1896    3979
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5875
 
PfoI T'CCnGG_A

Cuts at:   2644    2644 
   Size:       5875
 
PmeI GTTT'AAAC

Cuts at:   3179    3179 
   Size:       5875
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3944    3976    5457    5489    3944 
   Size:         32    1481      32    4330

  Fragments arranged by size: 

                4330    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   3005    3005 
   Size:       5875
 
PshAI GACnn'nnGTC

Cuts at:   1613    2657    1613 
   Size:       1044    4831
 
PsiI TTA'TAA

Cuts at:   3445    3445 
   Size:       5875
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:   2365    2397    2365 
   Size:         32    5843
 
PstI C_TGCA'G

Cuts at:    830    3227     830 
   Size:       2397    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5875
 
SalI G'TCGA_C

Cuts at:   1936    3217    1936 
   Size:       1281    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   2868    2868 
   Size:       5875
 
SbfI CC_TGCA'GG

Cuts at:   3227    3227 
   Size:       5875
 
ScaI AGT'ACT

Cuts at:   4123    5835    4123 
   Size:       1712    4163
 
SfcI C'TryA_G

Cuts at:    826    1045    2856    3223    4358    5039    5230     826 
   Size:        219    1811     367    1135     681     191    1471

  Fragments arranged by size: 

                1811    1471    1135     681     367     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5875
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5875
 
SmaI CCC'GGG

Cuts at:   1872    3202    1872 
   Size:       1330    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2791    3979    4880    5142     820 
   Size:        197     926     848    1188     901     262    1553

  Fragments arranged by size: 

                1553    1188     926     901     848     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3666     493 
   Size:       3173    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5875
 
SphI G_CATG'C

Cuts at:   1132    3233    1132 
   Size:       2101    3774
 
SspI AAT'ATT

Cuts at:      5      52    2593    3120    3799       5 
   Size:         47    2541     527     679    2081

  Fragments arranged by size: 

                2541    2081     679     527      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5875
 
StyI C'CwwG_G

Cuts at:    513    1065    3314     513 
   Size:        552    2249    3074

  Fragments arranged by size: 

                3074    2249     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2186    2413    3150    3902    3919    4072    4257 
   Size:       1255     606     227     737     752      17     153     185

Cuts at:   4257    4702     325 
   Size:        445    1498

  Fragments arranged by size: 

                1498    1255     752     737     606     445     227     185
                 153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2217    2297    4121 
   Size:        274      80      33      51    1443     240      80    1824

Cuts at:   4121    5833      96 
   Size:       1712     138

  Fragments arranged by size: 

                1824    1712    1443     274     240     138      80      80
                  51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5835    5867    5835 
   Size:         32    5843
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5875
 
XbaI T'CTAG_A

Cuts at:   3211    3211 
   Size:       5875
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2642    2642 
   Size:       5875
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5875
 
XmaI C'CCGG_G

Cuts at:   1870    3200    1870 
   Size:       1330    4545
 
XmnI GAAnn'nnTTC

Cuts at:   4004    4004 
   Size:       5875
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3683     276 
   Size:         53      83     186     936    2149    2468

  Fragments arranged by size: 

                2468    2149     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI    BbvCI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI
     EarI     EciI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII
    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NspI
     PasI     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI
     PsiI     PsrI     PstI     PvuI     SalI     SapI     SbfI     ScaI
     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AloI     ApaI     AscI    AvrII     BaeI     BbeI    BfrBI
     BplI    BsiWI    BsmBI  BstZ17I   Bsu36I   DraIII  EcoICRI    EcoNI
    EcoRV     FseI    FspAI     KasI     NaeI     NarI   NgoMIV     NruI
     NsiI     PacI     PmlI   PspOMI    PspXI    PvuII    RsrII     SacI
    SacII    SanDI    SexAI     SfiI     SfoI     SrfI     SwaI
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