################################## # NO CUT ENZYMES ( 107 enzymes) # ################################## AatII AccI AccIII AcyI AflII AflIII AgeI Alw44I Aor51HI ApaLI AscI AseI AsnI AspI AviII AvrII BalI BamHI BbeI BbrPI BcgI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BseAI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI Bst1107I BstZ17I CelII ClaI CpoI DraI DraIII DrdI EagI EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FseI FspI Hin1I HindIII HpaI KasI KspI MamI MfeI MluI MluNI MroI MscI MunI NarI NdeI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI PvuI RcaI RsrII SacII SalI SapI ScaI SexAI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SspBI SspI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### Acc65I 157 GGTACC G^GTACC AclI 550 AACGTT AA^CGTT AhdI 819 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp700I 511 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 157 GGTACC G^GTACC AspEI 819 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BanI 157 GGTACC G^G[CT][GA]CC BbsI 319 GAAGAC GAAGAC(2/6) BpuAI 319 GAAGAC GAAGAC(2/6) BsaWI 1743 ACCGGA [AT]^CCGG[AT] BsrFI 1179 GCCGGC [GA]^CCGG[CT] BssHII 56 GCGCGC G^CGCGC BssSI 337 CTCGTG CACGAG(-5/-1) BstBI 115 TTCGAA TT^CGAA BstXI 574 CCATCATTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 1056 GGATCT [GA]^GATC[CT] Cfr10I 1179 GCCGGC [GA]^CCGG[CT] DsaI 1805 CCATGG C^C[GA][CT]GG EaeI 2 TGGCCG [CT]^GGCC[GA] Eam1105I 819 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 1925 CCTTACAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HaeII 741 AGCGCC [GA]GCGC^[CT] HincII 553 GTTGAC GT[CT]^[GA]AC HindII 553 GTTGAC GT[CT]^[GA]AC KpnI 157 GGTACC GGTAC^C MflI 1056 GGATCT [GA]^GATC[CT] MslI 575 CATCATTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 1179 GCCGGC GCC^GGC NcoI 1805 CCATGG C^CATGG NgoMIV 1179 GCCGGC G^CCGGC NheI 1824 GCTAGC G^CTAGC NspI 206 GCATGC [GA]CATG^[CT] NspV 115 TTCGAA TT^CGAA Psp1406I 550 AACGTT AA^CGTT SbfI 1093 CCTGCAGG CCTGCA^GG SfuI 115 TTCGAA TT^CGAA SphI 206 GCATGC GCATG^C Sse8387I 1093 CCTGCAGG CCTGCA^GG XhoII 1056 GGATCT [GA]^GATC[CT] XmnI 511 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AcsI 514 GAATTT [GA]^AATT[CT] 1908 AAATTC AlwI 1069 GATCC GGATC(4/5) 1056 GGATC ApaI 1457 GGGCCC GGGCC^C 1725 GGGCCC ApoI 514 GAATTT [GA]^AATT[CT] 1908 AAATTC AspHI 468 GAGCTC G[AT]GC[AT]^C 1585 GAGCTC BciVI 363 GGATAC GTATCC(6/5) 1221 GGATAC BclI 1397 TGATCA T^GATCA 1974 TGATCA BcnI 1502 CCGGG CC^[GC]GG 1723 CCGGG BfaI 424 CTAG C^TAG 1825 CTAG BglI 715 GCCGACAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1804 GCCATGGGGGC BsaI 1699 GAGACC GGTCTC(1/5) 2030 GAGACC BsiHKAI 468 GAGCTC G[AT]GC[AT]^C 1585 GAGCTC BsmBI 624 CGTCTC CGTCTC(1/5) 851 CGTCTC BsrDI 768 CATTGC GCAATG(2/0) 1136 CATTGC BstAPI 1096 GCAGGTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1558 GCAGAGAGTGC BstEII 703 GGTGACC G^GT[ACGT]ACC 875 GGTCACC BstPI 703 GGTGACC G^GT[ACGT]ACC 875 GGTCACC EcoO65I 703 GGTGACC G^GT[ACGT]ACC 875 GGTCACC FbaI 1397 TGATCA T^GATCA 1974 TGATCA MaeI 424 CTAG C^TAG 1825 CTAG MspA1I 1424 CAGCTG C[AC]G^C[GT]G 1448 CAGCTG NciI 1502 CCGGG CC^[GC]GG 1723 CCGGG PflMI 75 CCAAAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 447 CCACCATCTGG PvuII 1424 CAGCTG CAG^CTG 1448 CAGCTG SacI 468 GAGCTC GAGCT^C 1585 GAGCTC Van91I 75 CCAAAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 447 CCACCATCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 85 enzymes) # ######################################### AccII AciI AfaI AluI AlwNI AocI AvaI AvaII BanII BbvCI BbvI BmyI BpmI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MnlI MseI MspI MvaI MvnI MwoI NdeII NlaIII NlaIV PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I