(Circular) (Six-base) MAPSORT of: pFN21AB5783.seq Check: 2233 from: 1 to: 6790 pFN21AB5783 6790 bp With 182 enzymes: SgfI * July 6, 2012 17:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2860 3106 3349 2860 Size: 246 243 6301 Fragments arranged by size: 6301 246 243 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4600 278 Size: 53 83 186 936 3064 2468 Fragments arranged by size: 3064 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4133 1937 Size: 2196 4594 Acc65I G'GTAC_C Cuts at: 2162 4111 2162 Size: 1949 4841 AclI AA'CG_TT Cuts at: 1563 2556 4917 5290 1563 Size: 993 2361 373 3063 Fragments arranged by size: 3063 2361 993 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2156 3600 4010 4853 6237 6505 1632 Size: 524 1444 410 843 1384 268 1917 Fragments arranged by size: 1917 1444 1384 843 524 410 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6593 AflIII A'CryG_T Cuts at: 1203 5689 6666 1203 Size: 4486 977 1327 Fragments arranged by size: 4486 1327 977 AgeI A'CCGG_T Cuts at: 5621 5621 Size: 6790 AhdI GACnn_n'nnGTC Cuts at: 2829 5519 2829 Size: 2690 4100 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6790 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6790 AlwNI CAG_nnn'CTG Cuts at: 3107 3455 3977 4533 6105 3107 Size: 348 522 556 1572 3792 Fragments arranged by size: 3792 1572 556 522 348 ApaI G_GGCC'C Cuts at: 3466 3734 3466 Size: 268 6522 ApaLI G'TGCA_C Cuts at: 4847 6003 4847 Size: 1156 5634 ApoI r'AATT_y Cuts at: 1493 2519 3913 4099 4325 4477 6586 1493 Size: 1026 1394 186 226 152 2109 1697 Fragments arranged by size: 2109 1697 1394 1026 226 186 152 AseI AT'TA_AT Cuts at: 160 1032 5344 160 Size: 872 4312 1606 Fragments arranged by size: 4312 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6790 AvaI C'yCGr_G Cuts at: 1870 1943 2815 3086 3372 4115 1870 Size: 73 872 271 286 743 4545 Fragments arranged by size: 4545 872 743 286 271 73 BamHI G'GATC_C Cuts at: 4120 4487 4120 Size: 367 6423 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2162 4111 5566 618 Size: 325 217 627 375 1949 1455 1842 Fragments arranged by size: 1949 1842 1455 627 375 325 217 BanII G_rGCy'C Cuts at: 1391 2477 2822 3466 3594 3699 3734 4109 1391 Size: 1086 345 644 128 105 35 375 4072 Fragments arranged by size: 4072 1086 644 375 345 128 105 35 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2331 928 Size: 971 432 5387 Fragments arranged by size: 5387 971 432 BbvCI CC'TCA_GC Cuts at: 3378 3456 3786 3378 Size: 78 330 6382 Fragments arranged by size: 6382 330 78 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4981 5015 1903 Size: 34 3044 34 3678 Fragments arranged by size: 3678 3044 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2362 3220 4682 5892 2362 Size: 858 1462 1210 3260 Fragments arranged by size: 3260 1462 1210 858 BclI T'GATC_A Cuts at: 1548 3402 3979 1548 Size: 1854 577 4359 Fragments arranged by size: 4359 1854 577 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2726 3815 5401 Size: 107 122 71 631 605 1054 1089 1586 Cuts at: 5401 136 Size: 1525 Fragments arranged by size: 1586 1525 1089 1054 631 605 122 107 71 BglII A'GATC_T Cuts at: 6785 6785 Size: 6790 BlpI GC'TnA_GC Cuts at: 4207 4207 Size: 6790 Bme1580I G_kGCm'C Cuts at: 1790 2449 3466 3574 3734 4851 6007 1790 Size: 659 1017 108 160 1117 1156 2573 Fragments arranged by size: 2573 1156 1117 1017 659 160 108 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6790 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2714 2745 4521 Size: 416 53 83 399 24 1297 31 1776 Cuts at: 4521 5479 442 Size: 958 1753 Fragments arranged by size: 1776 1753 1297 958 416 399 83 53 31 24 BmtI G_CTAG'C Cuts at: 1056 3833 1056 Size: 2777 4013 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3664 3664 Size: 6790 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2617 3132 3432 5450 1239 Size: 1378 515 300 2018 2579 Fragments arranged by size: 2579 2018 1378 515 300 Bpu10I CC'TnA_GC Cuts at: 3378 3456 3786 3378 Size: 78 330 6382 Fragments arranged by size: 6382 330 78 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4915 5780 6078 4915 Size: 865 298 5627 Fragments arranged by size: 5627 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3698 4029 5453 882 Size: 617 2199 331 1424 2219 Fragments arranged by size: 2219 2199 1424 617 331 BsaAI yAC'GTr Cuts at: 493 1204 4581 493 Size: 711 3377 2702 Fragments arranged by size: 3377 2702 711 BsaBI GATnn'nnATC Cuts at: 6784 6784 Size: 6790 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4597 4979 5614 275 Size: 53 83 186 936 3064 382 635 1451 Fragments arranged by size: 3064 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3748 5223 5621 5895 6042 6453 3748 Size: 1475 398 274 147 411 4085 Fragments arranged by size: 4085 1475 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6565 Fragments arranged by size: 6565 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2813 3062 3332 3395 3407 3542 3602 3714 Size: 1627 249 270 63 12 135 60 112 Cuts at: 3714 1186 Size: 4262 Fragments arranged by size: 4262 1627 270 249 135 112 63 60 12 BseYI C'CCAG_C Cuts at: 2086 5993 6489 2086 Size: 3907 496 2387 Fragments arranged by size: 3907 2387 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3582 3711 3786 1163 Size: 573 1846 129 75 4167 Fragments arranged by size: 4167 1846 573 129 75 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4240 5001 6029 1540 Size: 275 186 2239 761 1028 2301 Fragments arranged by size: 2301 2239 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2477 3594 4851 4936 6007 2477 Size: 1117 1257 85 1071 3260 Fragments arranged by size: 3260 1257 1117 1071 85 BsmI GAATG_Cn' Cuts at: 4301 4394 4301 Size: 93 6697 BsmBI CGTCTCn'nnnn_ Cuts at: 2635 2862 2635 Size: 227 6563 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2449 2477 2822 3466 3574 3594 3699 Size: 399 659 28 345 644 108 20 105 Cuts at: 3699 3734 4109 4851 4936 6007 1391 Size: 35 375 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 659 644 399 375 345 108 105 85 35 28 20 BspEI T'CCGG_A Cuts at: 6453 6453 Size: 6790 BspHI T'CATG_A Cuts at: 4679 6409 4679 Size: 1730 5060 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2860 3092 3106 3349 4145 844 Size: 2016 232 14 243 796 3489 Fragments arranged by size: 3489 2016 796 243 232 14 BsrBI CCG'CTC Cuts at: 1394 1823 4677 1394 Size: 429 2854 3507 Fragments arranged by size: 3507 2854 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2772 3140 5285 5459 65 Size: 1183 1524 368 2145 174 1396 Fragments arranged by size: 2145 1524 1396 1183 368 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3184 5434 5621 1700 Size: 110 1374 2250 187 2869 Fragments arranged by size: 2869 2250 1374 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6790 BssHII G'CGCG_C Cuts at: 1925 2061 1925 Size: 136 6654 BssSI C'ACGA_G Cuts at: 2342 4850 5862 2342 Size: 2508 1012 3270 Fragments arranged by size: 3270 2508 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3107 3569 794 Size: 2313 462 4015 Fragments arranged by size: 4015 2313 462 BstBI TT'CG_AA Cuts at: 2121 5594 2121 Size: 3473 3317 BstEII G'GTnAC_C Cuts at: 1184 2708 2880 1184 Size: 1524 172 5094 Fragments arranged by size: 5094 1524 172 BstXI CCAn_nnnn'nTGG Cuts at: 2586 2586 Size: 6790 BstYI r'GATC_y Cuts at: 1972 3061 4120 4487 4872 4889 6341 6427 6439 Size: 1089 1059 367 385 17 1452 86 12 Cuts at: 6439 6785 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1089 1059 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2796 3033 3609 2796 Size: 237 576 5977 Fragments arranged by size: 5977 576 237 BtgI C'CryG_G Cuts at: 513 1065 2004 3810 513 Size: 552 939 1806 3493 Fragments arranged by size: 3493 1806 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2392 487 Size: 683 56 1166 4885 Fragments arranged by size: 4885 1166 683 56 BtsI GCAGTG_nn' Cuts at: 3405 3890 4306 5100 5120 3405 Size: 485 416 794 20 5075 Fragments arranged by size: 5075 794 485 416 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6790 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6755 DraI TTT'AAA Cuts at: 4094 4094 Size: 6790 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5797 809 Size: 507 4481 1802 Fragments arranged by size: 4481 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 4237 5126 Size: 54 1425 50 275 18 177 2230 889 Cuts at: 5126 6755 8 Size: 1629 43 Fragments arranged by size: 2230 1629 1425 889 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4237 1537 Size: 275 2425 4090 Fragments arranged by size: 4090 2425 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2541 2565 3654 4720 1353 Size: 1188 24 1089 1066 3423 Fragments arranged by size: 3423 1188 1089 1066 24 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3500 5377 5751 5897 387 Size: 1064 138 1911 1877 374 146 1280 Fragments arranged by size: 1911 1877 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2475 3592 2475 Size: 1117 5673 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2156 2617 3132 3432 3600 4010 4853 Size: 393 524 461 515 300 168 410 843 Cuts at: 4853 5450 6237 6505 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 843 787 597 524 515 461 410 393 300 268 168 EcoNI CCTnn'n_nnAGG Cuts at: 3934 3934 Size: 6790 EcoO109I rG'GnC_Cy Cuts at: 2074 2352 2762 2903 3151 3462 3463 3731 3781 Size: 278 410 141 248 311 1 268 50 Cuts at: 3781 2074 Size: 5083 Fragments arranged by size: 5083 410 311 278 268 248 141 50 1 EcoRI G'AATT_C Cuts at: 4099 4477 4099 Size: 378 6412 FspI TGC'GCA Cuts at: 5296 5296 Size: 6790 HaeII r_GCGC'y Cuts at: 1678 2750 5937 1678 Size: 1072 3187 2531 Fragments arranged by size: 3187 2531 1072 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2564 2596 2815 2847 5431 Size: 32 135 32 992 32 219 32 2584 Cuts at: 5431 5463 5505 5537 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2584 992 219 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2560 4134 4380 669 Size: 988 281 622 1574 246 3079 Fragments arranged by size: 3079 1574 988 622 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6790 HpaI GTT'AAC Cuts at: 4380 4380 Size: 6790 KpnI G_GTAC'C Cuts at: 2166 4115 2166 Size: 1949 4841 MfeI C'AATT_G Cuts at: 4389 4389 Size: 6790 MluI A'CGCG_T Cuts at: 6666 6666 Size: 6790 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2581 3415 3675 5904 6088 1305 Size: 1276 834 260 2229 184 2007 Fragments arranged by size: 2229 2007 1276 834 260 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4968 Fragments arranged by size: 4968 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2584 4748 5107 5266 518 Size: 648 1418 2164 359 159 2042 Fragments arranged by size: 2164 2042 1418 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3431 3455 4206 4883 6031 6276 1606 Size: 1825 24 751 677 1148 245 2120 Fragments arranged by size: 2120 1825 1148 751 677 245 24 NaeI GCC'GGC Cuts at: 3186 3186 Size: 6790 NcoI C'CATG_G Cuts at: 513 1065 2004 3810 513 Size: 552 939 1806 3493 Fragments arranged by size: 3493 1806 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6790 NgoMIV G'CCGG_C Cuts at: 3184 3184 Size: 6790 NheI G'CTAG_C Cuts at: 1052 3829 1052 Size: 2777 4013 NotI GC'GGCC_GC Cuts at: 4237 4237 Size: 6790 NspI r_CATG'y Cuts at: 1132 1229 2215 4148 5693 1132 Size: 97 986 1933 1545 2229 Fragments arranged by size: 2229 1933 1545 986 97 PasI CC'CwG_GG Cuts at: 1348 3147 1348 Size: 1799 4991 PciI A'CATG_T Cuts at: 5689 5689 Size: 6790 PflMI CCAn_nnn'nTGG Cuts at: 1108 2086 2458 1108 Size: 978 372 5440 Fragments arranged by size: 5440 978 372 PfoI T'CCnGG_A Cuts at: 3777 3777 Size: 6790 PmeI GTTT'AAAC Cuts at: 4094 4094 Size: 6790 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4859 4891 6372 6404 4859 Size: 32 1481 32 5245 Fragments arranged by size: 5245 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2074 2352 2762 3151 3781 2074 Size: 278 410 389 630 5083 Fragments arranged by size: 5083 630 410 389 278 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6790 PsiI TTA'TAA Cuts at: 4360 4360 Size: 6790 PspOMI G'GGCC_C Cuts at: 3462 3730 3462 Size: 268 6522 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3915 3947 3915 Size: 32 6758 PstI C_TGCA'G Cuts at: 830 2472 2952 3103 4142 830 Size: 1642 480 151 1039 3478 Fragments arranged by size: 3478 1642 1039 480 151 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6790 PvuII CAG'CTG Cuts at: 3431 3455 3431 Size: 24 6766 SacI G_AGCT'C Cuts at: 2477 3594 2477 Size: 1117 5673 SalI G'TCGA_C Cuts at: 1936 4132 1936 Size: 2196 4594 SbfI CC_TGCA'GG Cuts at: 3103 4142 3103 Size: 1039 5751 ScaI AGT'ACT Cuts at: 5038 6750 5038 Size: 1712 5078 SfcI C'TryA_G Cuts at: 826 1045 2468 2948 3099 3684 4138 5273 5954 Size: 219 1423 480 151 585 454 1135 681 Cuts at: 5954 6145 826 Size: 191 1471 Fragments arranged by size: 1471 1423 1135 681 585 480 454 219 191 151 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6790 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6790 SmaI CCC'GGG Cuts at: 1872 4117 1872 Size: 2245 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4894 5795 6057 820 Size: 197 926 2951 901 262 1553 Fragments arranged by size: 2951 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4581 493 Size: 4088 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6790 SphI G_CATG'C Cuts at: 1132 2215 4148 1132 Size: 1083 1933 3774 Fragments arranged by size: 3774 1933 1083 SspI AAT'ATT Cuts at: 5 52 4714 5 Size: 47 4662 2081 Fragments arranged by size: 4662 2081 47 StuI AGG'CCT Cuts at: 1517 2222 2729 3848 4056 1517 Size: 705 507 1119 208 4251 Fragments arranged by size: 4251 1119 705 507 208 StyI C'CwwG_G Cuts at: 513 1065 2004 2740 3358 3441 3699 3810 4229 Size: 552 939 736 618 83 258 111 419 Cuts at: 4229 513 Size: 3074 Fragments arranged by size: 3074 939 736 618 552 419 258 111 83 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2696 2868 3142 3313 4817 4834 4987 Size: 1255 1116 172 274 171 1504 17 153 Cuts at: 4987 5172 5617 325 Size: 185 445 1498 Fragments arranged by size: 1504 1498 1255 1116 445 274 185 172 171 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4029 5036 6748 Size: 274 80 33 51 1443 2052 1007 1712 Cuts at: 6748 96 Size: 138 Fragments arranged by size: 2052 1712 1443 1007 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3429 3461 6750 6782 3429 Size: 32 3289 32 3437 Fragments arranged by size: 3437 3289 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6790 XbaI T'CTAG_A Cuts at: 4126 4126 Size: 6790 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6790 XmaI C'CCGG_G Cuts at: 1870 4115 1870 Size: 2245 4545 XmnI GAAnn'nnTTC Cuts at: 2520 4919 2520 Size: 2399 4391 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4598 276 Size: 53 83 186 936 3064 2468 Fragments arranged by size: 3064 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI