(Linear) (Six-base) MAPSORT of: KIBB5783.seq Check: 9478 from: 1 to: 2085 KIBB5783.orf With 182 enzymes: SgfI * July 3, 2012 16:06 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 855 1101 1344 2085 Size: 855 246 243 741 Fragments arranged by size: 855 741 246 243 Acc65I G'GTAC_C Cuts at: 0 157 2085 Size: 157 1928 AclI AA'CG_TT Cuts at: 0 551 2085 Size: 551 1534 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 151 1595 2005 2085 Size: 151 1444 410 80 Fragments arranged by size: 1444 410 151 80 AhdI GACnn_n'nnGTC Cuts at: 0 824 2085 Size: 824 1261 AlwNI CAG_nnn'CTG Cuts at: 0 1102 1450 1972 2085 Size: 1102 348 522 113 Fragments arranged by size: 1102 522 348 113 ApaI G_GGCC'C Cuts at: 0 1461 1729 2085 Size: 1461 268 356 Fragments arranged by size: 1461 356 268 ApoI r'AATT_y Cuts at: 0 514 1908 2085 Size: 514 1394 177 Fragments arranged by size: 1394 514 177 AvaI C'yCGr_G Cuts at: 0 810 1081 1367 2085 Size: 810 271 286 718 Fragments arranged by size: 810 718 286 271 BanI G'GyrC_C Cuts at: 0 157 2085 Size: 157 1928 BanII G_rGCy'C Cuts at: 0 472 817 1461 1589 1694 1729 2085 Size: 472 345 644 128 105 35 356 Fragments arranged by size: 644 472 356 345 128 105 35 BbsI GAAGACnn'nnnn_ Cuts at: 0 326 2085 Size: 326 1759 BbvCI CC'TCA_GC Cuts at: 0 1373 1451 1781 2085 Size: 1373 78 330 304 Fragments arranged by size: 1373 330 304 78 BciVI GTATCCnnnnn_n' Cuts at: 0 357 1215 2085 Size: 357 858 870 Fragments arranged by size: 870 858 357 BclI T'GATC_A Cuts at: 0 1397 1974 2085 Size: 1397 577 111 Fragments arranged by size: 1397 577 111 BglI GCCn_nnn'nGGC Cuts at: 0 721 1810 2085 Size: 721 1089 275 Fragments arranged by size: 1089 721 275 Bme1580I G_kGCm'C Cuts at: 0 444 1461 1569 1729 2085 Size: 444 1017 108 160 356 Fragments arranged by size: 1017 444 356 160 108 BmrI ACTGGGnnnn_n' Cuts at: 0 709 740 2085 Size: 709 31 1345 Fragments arranged by size: 1345 709 31 BmtI G_CTAG'C Cuts at: 0 1828 2085 Size: 1828 257 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1659 2085 Size: 1659 426 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 612 1127 1427 2085 Size: 612 515 300 658 Fragments arranged by size: 658 612 515 300 Bpu10I CC'TnA_GC Cuts at: 0 1373 1451 1781 2085 Size: 1373 78 330 304 Fragments arranged by size: 1373 330 304 78 BsaI GGTCTCn'nnnn_ Cuts at: 0 1693 2024 2085 Size: 1693 331 61 Fragments arranged by size: 1693 331 61 BsaWI w'CCGG_w Cuts at: 0 1743 2085 Size: 1743 342 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 808 1057 1327 1390 1402 1537 1597 1709 Size: 808 249 270 63 12 135 60 112 Cuts at: 1709 2085 Size: 376 Fragments arranged by size: 808 376 270 249 135 112 63 60 12 BseYI C'CCAG_C Cuts at: 0 81 2085 Size: 81 2004 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1577 1706 1781 2085 Size: 1577 129 75 304 Fragments arranged by size: 1577 304 129 75 BsiHKAI G_wGCw'C Cuts at: 0 472 1589 2085 Size: 472 1117 496 Fragments arranged by size: 1117 496 472 BsmBI CGTCTCn'nnnn_ Cuts at: 0 630 857 2085 Size: 630 227 1228 Fragments arranged by size: 1228 630 227 Bsp1286I G_dGCh'C Cuts at: 0 444 472 817 1461 1569 1589 1694 1729 Size: 444 28 345 644 108 20 105 35 Cuts at: 1729 2085 Size: 356 Fragments arranged by size: 644 444 356 345 108 105 35 28 20 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 855 1087 1101 1344 2085 Size: 855 232 14 243 741 Fragments arranged by size: 855 741 243 232 14 BsrDI GCAATG_nn' Cuts at: 0 767 1135 2085 Size: 767 368 950 Fragments arranged by size: 950 767 368 BsrFI r'CCGG_y Cuts at: 0 1179 2085 Size: 1179 906 BssHII G'CGCG_C Cuts at: 0 56 2085 Size: 56 2029 BssSI C'ACGA_G Cuts at: 0 337 2085 Size: 337 1748 BstAPI GCAn_nnn'nTGC Cuts at: 0 1102 1564 2085 Size: 1102 462 521 Fragments arranged by size: 1102 521 462 BstBI TT'CG_AA Cuts at: 0 116 2085 Size: 116 1969 BstEII G'GTnAC_C Cuts at: 0 703 875 2085 Size: 703 172 1210 Fragments arranged by size: 1210 703 172 BstXI CCAn_nnnn'nTGG Cuts at: 0 581 2085 Size: 581 1504 BstYI r'GATC_y Cuts at: 0 1056 2085 Size: 1056 1029 Bsu36I CC'TnA_GG Cuts at: 0 791 1028 1604 2085 Size: 791 237 576 481 Fragments arranged by size: 791 576 481 237 BtgI C'CryG_G Cuts at: 0 1805 2085 Size: 1805 280 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 387 2085 Size: 387 1698 BtsI GCAGTG_nn' Cuts at: 0 1400 1885 2085 Size: 1400 485 200 Fragments arranged by size: 1400 485 200 EaeI y'GGCC_r Cuts at: 0 2 2085 Size: 2 2083 EarI CTCTTCn'nnn_ Cuts at: 0 536 560 1649 2085 Size: 536 24 1089 436 Fragments arranged by size: 1089 536 436 24 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1495 2085 Size: 1495 590 EcoICRI GAG'CTC Cuts at: 0 470 1587 2085 Size: 470 1117 498 Fragments arranged by size: 1117 498 470 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 151 612 1127 1427 1595 2005 2085 Size: 151 461 515 300 168 410 80 Fragments arranged by size: 515 461 410 300 168 151 80 EcoNI CCTnn'n_nnAGG Cuts at: 0 1929 2085 Size: 1929 156 EcoO109I rG'GnC_Cy Cuts at: 0 69 347 757 898 1146 1457 1458 1726 Size: 69 278 410 141 248 311 1 268 Cuts at: 1726 1776 2085 Size: 50 309 Fragments arranged by size: 410 311 309 278 268 248 141 69 50 1 HaeII r_GCGC'y Cuts at: 0 745 2085 Size: 745 1340 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 559 591 810 842 2085 Size: 559 32 219 32 1243 Fragments arranged by size: 1243 559 219 32 32 HincII GTy'rAC Cuts at: 0 555 2085 Size: 555 1530 KpnI G_GTAC'C Cuts at: 0 161 2085 Size: 161 1924 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 576 1410 1670 2085 Size: 576 834 260 415 Fragments arranged by size: 834 576 415 260 MslI CAynn'nnrTG Cuts at: 0 579 2085 Size: 579 1506 MspA1I CmG'CkG Cuts at: 0 1426 1450 2085 Size: 1426 24 635 Fragments arranged by size: 1426 635 24 NaeI GCC'GGC Cuts at: 0 1181 2085 Size: 1181 904 NcoI C'CATG_G Cuts at: 0 1805 2085 Size: 1805 280 NgoMIV G'CCGG_C Cuts at: 0 1179 2085 Size: 1179 906 NheI G'CTAG_C Cuts at: 0 1824 2085 Size: 1824 261 NspI r_CATG'y Cuts at: 0 210 2085 Size: 210 1875 PasI CC'CwG_GG Cuts at: 0 1142 2085 Size: 1142 943 PflMI CCAn_nnn'nTGG Cuts at: 0 81 453 2085 Size: 81 372 1632 Fragments arranged by size: 1632 372 81 PfoI T'CCnGG_A Cuts at: 0 1772 2085 Size: 1772 313 PpuMI rG'GwC_Cy Cuts at: 0 69 347 757 1146 1776 2085 Size: 69 278 410 389 630 309 Fragments arranged by size: 630 410 389 309 278 69 PspOMI G'GGCC_C Cuts at: 0 1457 1725 2085 Size: 1457 268 360 Fragments arranged by size: 1457 360 268 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1910 1942 2085 Size: 1910 32 143 Fragments arranged by size: 1910 143 32 PstI C_TGCA'G Cuts at: 0 467 947 1098 2085 Size: 467 480 151 987 Fragments arranged by size: 987 480 467 151 PvuII CAG'CTG Cuts at: 0 1426 1450 2085 Size: 1426 24 635 Fragments arranged by size: 1426 635 24 SacI G_AGCT'C Cuts at: 0 472 1589 2085 Size: 472 1117 496 Fragments arranged by size: 1117 496 472 SbfI CC_TGCA'GG Cuts at: 0 1098 2085 Size: 1098 987 SfcI C'TryA_G Cuts at: 0 463 943 1094 1679 2085 Size: 463 480 151 585 406 Fragments arranged by size: 585 480 463 406 151 SphI G_CATG'C Cuts at: 0 210 2085 Size: 210 1875 StuI AGG'CCT Cuts at: 0 217 724 1843 2051 2085 Size: 217 507 1119 208 34 Fragments arranged by size: 1119 507 217 208 34 StyI C'CwwG_G Cuts at: 0 735 1353 1436 1694 1805 2085 Size: 735 618 83 258 111 280 Fragments arranged by size: 735 618 280 258 111 83 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 691 863 1137 1308 2085 Size: 691 172 274 171 777 Fragments arranged by size: 777 691 274 172 171 TatI w'GTAC_w Cuts at: 0 2024 2085 Size: 2024 61 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1424 1456 2085 Size: 1424 32 629 Fragments arranged by size: 1424 629 32 XmnI GAAnn'nnTTC Cuts at: 0 515 2085 Size: 515 1570 Enzymes that do cut: AarI Acc65I AclI AcuI AhdI AlwNI ApaI ApoI AvaI BanI BanII BbsI BbvCI BciVI BclI BglI Bme1580I BmrI BmtI BplI BpmI Bpu10I BsaI BsaWI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII KpnI MmeI MslI MspA1I NaeI NcoI NgoMIV NheI NspI PasI PflMI PfoI PpuMI PspOMI PsrI PstI PvuII SacI SbfI SfcI SphI StuI StyI TaqII TatI TstI XmnI Enzymes that do not cut: AatII AccI AfeI AflII AflIII AgeI AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BcgI BfrBI BglII BlpI BmgBI BpuEI BsaAI BsaBI BsaHI BsaXI BsiEI BsiWI BsmI BspEI BspHI BsrBI BsrGI BstZ17I ClaI CspCI DraI DraIII DrdI EagI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KasI MfeI MluI MscI NarI NdeI NotI NruI NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI PvuI RsrII SacII SalI SanDI SapI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XcmI XhoI XmaI ZraI