################################## # NO CUT ENZYMES ( 160 enzymes) # ################################## AatII Acc65I AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsoBI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoO109I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeII MamI MfeI MluI MseI MunI NaeI NarI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp509I TspRI Tth111I Van91I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccI 48 GTATAC GT^[AC][GT]AC AccII 72 CGCG CG^CG AccIII 437 TCCGGA T^CCGGA AciI 447 GCGG CCGC(-3/-1) AlwI 297 GGATC GGATC(4/5) AocI 58 CCTGAGG CC^T[ACGT]AGG BalI 428 TGGCCA TGG^CCA BanII 235 GGGCTC G[GA]GC[CT]^C BbsI 393 GAAGAC GAAGAC(2/6) BfaI 172 CTAG C^TAG BmyI 235 GGGCTC G[AGT]GC[ACT]^C BpuAI 393 GAAGAC GAAGAC(2/6) BsaWI 437 TCCGGA [AT]^CCGG[AT] BseAI 437 TCCGGA T^CCGGA BsmI 518 GAATGC GAATGC(1/-1) Bsp1286I 235 GGGCTC G[AGT]GC[ACT]^C BspEI 437 TCCGGA T^CCGGA BspMI 142 GCAGGT ACCTGC(4/8) BsrI 294 ACTGG ACTGG(1/-1) Bst1107I 48 GTATAC GTA^TAC BstEII 145 GGTGACC G^GT[ACGT]ACC BstPI 145 GGTGACC G^GT[ACGT]ACC BstUI 72 CGCG CG^CG BstXI 257 CCAACCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 297 GGATCT [GA]^GATC[CT] BstZ17I 48 GTATAC GTA^TAC Bsu36I 58 CCTGAGG CC^T[ACGT]AGG Eco81I 58 CCTGAGG CC^T[ACGT]AGG EcoNI 4 CCTAAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 145 GGTGACC G^GT[ACGT]ACC EcoT14I 407 CCAAGG C^C[AT][AT]GG FauI 447 GCGGG CCCGC(4/6) HgaI 70 GACGC GACGC(5/10) HinfI 175 GACTC G^A[ACGT]TC HphI 145 GGTGA GGTGA(8/7) MaeI 172 CTAG C^TAG MflI 297 GGATCT [GA]^GATC[CT] MluNI 428 TGGCCA TGG^CCA MroI 437 TCCGGA T^CCGGA MscI 428 TGGCCA TGG^CCA MslI 372 CATGAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 72 CGCG CG^CG NlaIII 372 CATG CATG^ PleI 175 GACTC GAGTC(4/5) PvuII 76 CAGCTG CAG^CTG SfaNI 496 GATGC GCATC(5/9) StyI 407 CCAAGG C^C[AT][AT]GG XbaI 171 TCTAGA T^CTAGA XcmI 407 CCAAGGCTGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 297 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AluI 77 AGCT AG^CT 317 AGCT BglI 26 GCCACAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 421 GCCCGGCTGGC BsaJI 101 CCAGGG C^C[ACGT][ACGT]GG 407 CCAAGG BstF5I 435 CATCC GGATG(2/0) 192 GGATG BstNI 101 CCAGG CC^[AT]GG 417 CCTGG CfoI 73 GCGC GCG^C 219 GCGC Cfr13I 307 GGGCC G^G[ACGT]CC 420 GGCCC DpnII 66 GATC ^GATC 298 GATC EaeI 386 TGGCCG [CT]^GGCC[GA] 428 TGGCCA EcoRII 101 CCAGG ^CC[AT]GG 417 CCTGG FokI 435 CATCC GGATG(9/13) 192 GGATG HhaI 73 GCGC GCG^C 219 GCGC HinP1I 73 GCGC G^CGC 219 GCGC MboI 66 GATC ^GATC 298 GATC MspA1I 76 CAGCTG C[AC]G^C[GT]G 445 CAGCGG MvaI 101 CCAGG CC^[AT]GG 417 CCTGG NdeII 66 GATC ^GATC 298 GATC PstI 79 CTGCAG CTGCA^G 140 CTGCAG Sau3AI 66 GATC ^GATC 298 GATC Sau96I 307 GGGCC G^G[ACGT]CC 420 GGCCC SfcI 79 CTGCAG C^T[GA][CT]AG 140 CTGCAG SfiI 25 GGCCACAAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 420 GGCCCGGCTGGCC ######################################### # 3 OR MORE CUT ENZYMES ( 26 enzymes) # ######################################### BbvI BcnI BseRI BsiYI BslI BssKI Cac8I DdeI EarI Fnu4HI HaeIII HapII HpaII ItaI Ksp632I MaeIII MboII MnlI MspI MwoI NciI ScrFI TaqI TseI Tsp45I TthHB8I