Restriction map for pFN21AE2182
 (Circular) (Six-base) MAPSORT of: pFN21AE2182.seq  Check: 9938  from: 1  to: 5248



pFN21AE2182  5248 bp

 With 182 enzymes: SgfI * 

                        July 6, 2012 17:36  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2138    2138 
   Size:       5248
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3058     278 
   Size:         53      83     186     936    1522    2468

  Fragments arranged by size: 

                2468    1522     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2054    2591    1937 
   Size:        117     537    4594

  Fragments arranged by size: 

                4594     537     117
 
Acc65I G'GTAC_C

Cuts at:   2569    2569 
   Size:       5248
 
AclI AA'CG_TT

Cuts at:   1563    3375    3748    1563 
   Size:       1812     373    3063

  Fragments arranged by size: 

                3063    1812     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3311    4695    4963    1632 
   Size:       1679    1384     268    1917

  Fragments arranged by size: 

                1917    1679    1384     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5051
 
AflIII A'CryG_T

Cuts at:   1203    4147    5124    1203 
   Size:       2944     977    1327

  Fragments arranged by size: 

                2944    1327     977
 
AgeI A'CCGG_T

Cuts at:   4079    4079 
   Size:       5248
 
AhdI GACnn_n'nnGTC

Cuts at:   3977    3977 
   Size:       5248
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5248
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5248
 
AlwNI CAG_nnn'CTG

Cuts at:   2991    4563    2991 
   Size:       1572    3676
 
ApaLI G'TGCA_C

Cuts at:   3305    4461    3305 
   Size:       1156    4092
 
ApoI r'AATT_y

Cuts at:   1493    2557    2783    2935    5044    1493 
   Size:       1064     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1064     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3802     160 
   Size:        872    2770    1606

  Fragments arranged by size: 

                2770    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5248
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2573    1870 
   Size:         73     630    4545

  Fragments arranged by size: 

                4545     630      73
 
BamHI G'GATC_C

Cuts at:   2578    2945    2578 
   Size:        367    4881
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2569    4024     618 
   Size:        325     217     627     782    1455    1842

  Fragments arranged by size: 

                1842    1455     782     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2244    2567    1391 
   Size:        853     323    4072

  Fragments arranged by size: 

                4072     853     323
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2405     928 
   Size:        971     506    3771

  Fragments arranged by size: 

                3771     971     506
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    1987    2021    3439    3473    1903 
   Size:         34      50      34    1418      34    3678

  Fragments arranged by size: 

                3678    1418      50      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3140    4350    3140 
   Size:       1210    4038
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5248
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2037    2432    3859 
   Size:        107     122      71     631     605     365     395    1427

Cuts at:   3859     136 
   Size:       1525

  Fragments arranged by size: 

                1525    1427     631     605     395     365     122     107
                  71
 
BglII A'GATC_T

Cuts at:   5243    5243 
   Size:       5248
 
BlpI GC'TnA_GC

Cuts at:   2665    2665 
   Size:       5248
 
Bme1580I G_kGCm'C

Cuts at:   1790    3309    4465    1790 
   Size:       1519    1156    2573

  Fragments arranged by size: 

                2573    1519    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5248
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2979    3937     442 
   Size:        416      53      83     399      24    1562     958    1753

  Fragments arranged by size: 

                1753    1562     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5248
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    3908    1239 
   Size:       2669    2579
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3373    4238    4536    3373 
   Size:        865     298    4085

  Fragments arranged by size: 

                4085     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    3911     882 
   Size:        617    2412    2219

  Fragments arranged by size: 

                2412    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3039     493 
   Size:        711    1835    2702

  Fragments arranged by size: 

                2702    1835     711
 
BsaBI GATnn'nnATC

Cuts at:   5242    5242 
   Size:       5248
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3055    3437    4072     275 
   Size:         53      83     186     936    1522     382     635    1451

  Fragments arranged by size: 

                1522    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   2442    3681    4079    4353    4500    4911    2442 
   Size:       1239     398     274     147     411    2779

  Fragments arranged by size: 

                2779    1239     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5023

  Fragments arranged by size: 

                5023     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2081    2132    2344    2471    1186 
   Size:        895      51     212     127    3963

  Fragments arranged by size: 

                3963     895     212     127      51
 
BseYI C'CCAG_C

Cuts at:   4451    4947    4451 
   Size:        496    4752
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4675
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2698    3459    4487    1540 
   Size:        275     186     697     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     697     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3309    3394    4465    3309 
   Size:         85    1071    4092

  Fragments arranged by size: 

                4092    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2529    2759    2852    2529 
   Size:        230      93    4925

  Fragments arranged by size: 

                4925     230      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2244    2567    3309    3394    4465    1391 
   Size:        399     454     323     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     454     399     323      85
 
BspEI T'CCGG_A

Cuts at:   2442    4911    2442 
   Size:       2469    2779
 
BspHI T'CATG_A

Cuts at:   3137    4867    3137 
   Size:       1730    3518
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2138    2603     844 
   Size:       1294     465    3489

  Fragments arranged by size: 

                3489    1294     465
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3135    1394 
   Size:        429    1312    3507

  Fragments arranged by size: 

                3507    1312     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3743    3917      65 
   Size:       1183    2495     174    1396

  Fragments arranged by size: 

                2495    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3892    4079    1700 
   Size:        110    2082     187    2869

  Fragments arranged by size: 

                2869    2082     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5248
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5248
 
BssSI C'ACGA_G

Cuts at:   3308    4320    3308 
   Size:       1012    4236
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5248
 
BstBI TT'CG_AA

Cuts at:   4052    4052 
   Size:       5248
 
BstEII G'GTnAC_C

Cuts at:   1184    2150    1184 
   Size:        966    4282
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2269    2269 
   Size:       5248
 
BstYI r'GATC_y

Cuts at:   1972    2302    2578    2945    3330    3347    4799    4885    4897 
   Size:        330     276     367     385      17    1452      86      12

Cuts at:   4897    5243    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     385     367     346     330     276      86
                  17      12
 
BstZ17I GTA'TAC

Cuts at:   2055    2055 
   Size:       5248
 
Bsu36I CC'TnA_GG

Cuts at:   2064    2064 
   Size:       5248
 
BtgI C'CryG_G

Cuts at:    513    1065     513 
   Size:        552    4696
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4509

  Fragments arranged by size: 

                4509     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2764    3558    3578    2764 
   Size:        794      20    4434

  Fragments arranged by size: 

                4434     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5248
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5213
 
DraI TTT'AAA

Cuts at:   2552    2552 
   Size:       5248
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4255     809 
   Size:        507    2939    1802

  Fragments arranged by size: 

                2939    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2391    2433    2695 
   Size:         54    1425      50     275      18     561      42     262

Cuts at:   2695    3584    5213       8 
   Size:        889    1629      43

  Fragments arranged by size: 

                1629    1425     889     561     275     262      54      50
                  43      42      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2695    1537 
   Size:        275     883    4090

  Fragments arranged by size: 

                4090     883     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2109    2337    2472    3178    1353 
   Size:        756     228     135     706    3423

  Fragments arranged by size: 

                3423     756     706     228     135
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3835    4209    4355     387 
   Size:       1064     138    2246     374     146    1280

  Fragments arranged by size: 

                2246    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    3311    3908    4695    4963    1239 
   Size:        393    1679     597     787     268    1524

  Fragments arranged by size: 

                1679    1524     787     597     393     268
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2013    2543    2013 
   Size:        530    4718
 
EcoRI G'AATT_C

Cuts at:   2557    2935    2557 
   Size:        378    4870
 
FspI TGC'GCA

Cuts at:   3754    3754 
   Size:       5248
 
HaeII r_GCGC'y

Cuts at:   1678    4395    1678 
   Size:       2717    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2156    2188    3889    3921    3963 
   Size:         32     135      32     584      32    1701      32      42

Cuts at:   3963    3995    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    1701     584     135      42      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2592    2838     669 
   Size:        988     281     654     246    3079

  Fragments arranged by size: 

                3079     988     654     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5248
 
HpaI GTT'AAC

Cuts at:   2838    2838 
   Size:       5248
 
KpnI G_GTAC'C

Cuts at:   2573    2573 
   Size:       5248
 
MfeI C'AATT_G

Cuts at:   2847    2847 
   Size:       5248
 
MluI A'CGCG_T

Cuts at:   5124    5124 
   Size:       5248
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4362    4546    1305 
   Size:       3057     184    2007

  Fragments arranged by size: 

                3057    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2435      10 
   Size:         54    1425     343     603    2823

  Fragments arranged by size: 

                2823    1425     603     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2381    3206    3565    3724     518 
   Size:        648    1215     825     359     159    2042

  Fragments arranged by size: 

                2042    1215     825     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2083    2452    2664    3341    4489    4734    1606 
   Size:        477     369     212     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     677     477     369     245     212
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    4696
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5248
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5248
 
NotI GC'GGCC_GC

Cuts at:   2695    2695 
   Size:       5248
 
NspI r_CATG'y

Cuts at:   1132    1229    2606    4151    1132 
   Size:         97    1377    1545    2229

  Fragments arranged by size: 

                2229    1545    1377      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5248
 
PciI A'CATG_T

Cuts at:   4147    4147 
   Size:       5248
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5248
 
PmeI GTTT'AAAC

Cuts at:   2552    2552 
   Size:       5248
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3317    3349    4830    4862    3317 
   Size:         32    1481      32    3703

  Fragments arranged by size: 

                3703    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5248
 
PsiI TTA'TAA

Cuts at:   2818    2818 
   Size:       5248
 
PstI C_TGCA'G

Cuts at:    830    2088    2149    2600     830 
   Size:       1258      61     451    3478

  Fragments arranged by size: 

                3478    1258     451      61
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5248
 
PvuII CAG'CTG

Cuts at:   2083    2083 
   Size:       5248
 
SalI G'TCGA_C

Cuts at:   1936    2590    1936 
   Size:        654    4594
 
SbfI CC_TGCA'GG

Cuts at:   2600    2600 
   Size:       5248
 
ScaI AGT'ACT

Cuts at:   3496    5208    3496 
   Size:       1712    3536
 
SfcI C'TryA_G

Cuts at:    826    1045    2084    2145    2596    3731    4412    4603     826 
   Size:        219    1039      61     451    1135     681     191    1471

  Fragments arranged by size: 

                1471    1135    1039     681     451     219     191      61
            
 
SfiI GGCCn_nnn'nGGCC

Cuts at:   2037    2432    2037 
   Size:        395    4853
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5248
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5248
 
SmaI CCC'GGG

Cuts at:   1872    2575    1872 
   Size:        703    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3352    4253    4515     820 
   Size:        197     926    1409     901     262    1553

  Fragments arranged by size: 

                1553    1409     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3039     493 
   Size:       2546    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5248
 
SphI G_CATG'C

Cuts at:   1132    2606    1132 
   Size:       1474    3774
 
SspI AAT'ATT

Cuts at:      5      52    3172       5 
   Size:         47    3120    2081

  Fragments arranged by size: 

                3120    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5248
 
StyI C'CwwG_G

Cuts at:    513    1065    2412    2687     513 
   Size:        552    1347     275    3074

  Fragments arranged by size: 

                3074    1347     552     275
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3275    3292    3445    3630    4075     325 
   Size:       1255    1695      17     153     185     445    1498

  Fragments arranged by size: 

                1695    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3494    5206      96 
   Size:        274      80      33      51    1443    1517    1712     138

  Fragments arranged by size: 

                1712    1517    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5208    5240    5208 
   Size:         32    5216
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5248
 
XbaI T'CTAG_A

Cuts at:   2176    2584    2176 
   Size:        408    4840
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2419    2419 
   Size:       5248
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5248
 
XmaI C'CCGG_G

Cuts at:   1870    2573    1870 
   Size:        703    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3377    3377 
   Size:       5248
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3056     276 
   Size:         53      83     186     936    1522    2468

  Fragments arranged by size: 

                2468    1522     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI
     EarI     EciI  Eco57MI    EcoNI    EcoRI     FspI    HaeII    Hin4I
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NspI     PasI
     PciI    PflMI     PmeI     PpiI    PshAI     PsiI     PstI     PvuI
    PvuII     SalI     SbfI     ScaI     SfcI     SfiI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI    BbvCI
    BfrBI     BplI   Bpu10I    BsiWI    BsmBI   DraIII  EcoICRI EcoO109I
    EcoRV     FalI     FseI    FspAI     KasI     NaeI     NarI   NgoMIV
     NruI     NsiI     PacI     PfoI     PmlI    PpuMI   PspOMI    PspXI
     PsrI    RsrII     SacI    SacII    SanDI     SapI    SexAI     SfoI
     SrfI     SwaI
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