Restriction map for
pFN21AE1971
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspI
AviII AvrII BamHI BbeI BcgI
BfrI BglI BlnI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII
BstPI BstUI BstXI Bsu36I CfoI
Cfr10I ClaI CpoI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRV EcoT22I
FauI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MroI MspA1I
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PflFI PinAI
PmeI PpuMI PshAI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 66 enzymes) #
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AccI 1280 GTATAC GT^[AC][GT]AC
AfaI 1437 GTAC GT^AC
AlwNI 1456 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 382 ATTAAT AT^TAAT
AsnI 382 ATTAAT AT^TAAT
Asp700I 1000 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 85 GAGCAC G[AT]GC[AT]^C
AvaII 1083 GGACC G^G[AT]CC
BalI 1385 TGGCCA TGG^CCA
BanI 129 GGTGCC G^G[CT][GA]CC
BanII 461 GGGCTC G[GA]GC[CT]^C
BbrPI 88 CACGTG CAC^GTG
BbsI 1600 GTCTTC GAAGAC(2/6)
BbvCI 1567 CCTCAGC CCTCAGC(-5/-2)
BclI 887 TGATCA T^GATCA
BcnI 1356 CCCGG CC^[GC]GG
BfaI 419 CTAG C^TAG
BglII 228 AGATCT A^GATCT
BlpI 82 GCTGAGC GC^T[ACGT]AGC
BpmI 319 CTCCAG CTGGAG(16/14)
Bpu1102I 82 GCTGAGC GC^T[ACGT]AGC
BpuAI 1600 GTCTTC GAAGAC(2/6)
BsaAI 88 CACGTG [CT]AC^GT[GA]
BsgI 1035 CTGCAC GTGCAG(16/14)
BsiHKAI 85 GAGCAC G[AT]GC[AT]^C
BsrBI 146 GAGCGG CCGCTC(-3/-3)
Bst1107I 1280 GTATAC GTA^TAC
BstYI 228 AGATCT [GA]^GATC[CT]
BstZ17I 1280 GTATAC GTA^TAC
Cac8I 686 GCCTGC GC[ACGT]^[ACGT]GC
CelII 82 GCTGAGC GC^T[ACGT]AGC
DraI 169 TTTAAA TTT^AAA
DraII 1166 AGGCCCC [GA]G^G[ACGT]CC[CT]
DsaI 1125 CCATGG C^C[GA][CT]GG
EaeI 1385 TGGCCA [CT]^GGCC[GA]
EcoO109I 1166 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRI 715 GAATTC G^AATTC
EcoT14I 1125 CCATGG C^C[AT][AT]GG
FbaI 887 TGATCA T^GATCA
HapII 1357 CCGG C^CGG
HpaII 1357 CCGG C^CGG
MaeI 419 CTAG C^TAG
MaeII 89 ACGT A^CGT
MflI 228 AGATCT [GA]^GATC[CT]
MluNI 1385 TGGCCA TGG^CCA
MscI 1385 TGGCCA TGG^CCA
MspI 1357 CCGG C^CGG
NciI 1356 CCCGG CC^[GC]GG
NcoI 1125 CCATGG C^CATGG
PaeR7I 1478 CTCGAG C^TCGAG
PflMI 1074 CCAACTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 88 CACGTG CAC^GTG
PmlI 88 CACGTG CAC^GTG
PshBI 382 ATTAAT AT^TAAT
PstI 1421 CTGCAG CTGCA^G
RsaI 1437 GTAC GT^AC
SexAI 1432 ACCTGGT A^CC[AT]GGT
StyI 1125 CCATGG C^C[AT][AT]GG
TaqI 1479 TCGA T^CGA
Tsp45I 1446 GTCAC ^GT[GC]AC
TthHB8I 1479 TCGA T^CGA
Van91I 1074 CCAACTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 563 CCAAATGCAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1478 CTCGAG C^TCGAG
XhoII 228 AGATCT [GA]^GATC[CT]
XmnI 1000 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AciI 268 CCGC CCGC(-3/-1)
148 GCGG
AvaI 872 CTCGGG C^[CT]CG[GA]G
1478 CTCGAG
BciVI 877 GGATAC GTATCC(6/5)
1115 GTATCC
BsmFI 110 GGGAC GGGAC(10/14)
950 GGGAC
BsoBI 872 CTCGGG C^[CT]CG[GA]G
1478 CTCGAG
BspMI 590 ACCTGC ACCTGC(4/8)
101 GCAGGT
Cfr13I 1083 GGACC G^G[ACGT]CC
1167 GGCCC
HphI 1336 TCACC GGTGA(8/7)
1496 TCACC
MaeIII 1091 GTAAC ^GT[ACGT]AC
1446 GTCAC
MslI 606 CATAGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
967 CACTTGGATG
MwoI 270 GCAAAGGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1103 GCTTCATCTGC
Sau96I 1083 GGACC G^G[ACGT]CC
1167 GGCCC
SfcI 477 CTACAG C^T[GA][CT]AG
1421 CTGCAG
SmlI 241 CTCAAG C^T[CT][GA]AG
1478 CTCGAG
TfiI 829 GAATC G^A[AT]TC
1297 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AcsI AluI ApoI BbvI BmyI
BsaJI BseRI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
DdeI DpnII EarI EcoRII Fnu4HI
FokI HaeIII HinfI ItaI Ksp632I
MboI MboII MnlI MseI MvaI
NdeII NlaIII NlaIV PleI SapI
Sau3AI ScrFI SfaNI Tru9I TseI
Tsp509I TspRI