(Circular) (Six-base) MAPSORT of: pFN21AE1971.seq Check: 3537 from: 1 to: 6319 pFN21AE1971 6319 bp With 182 enzymes: SgfI * October 25, 2019 12:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4129 278 Size: 53 83 186 936 2593 2468 Fragments arranged by size: 2593 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3286 3662 1937 Size: 1349 376 4594 Fragments arranged by size: 4594 1349 376 Acc65I G'GTAC_C Cuts at: 3640 3640 Size: 6319 AclI AA'CG_TT Cuts at: 1563 4446 4819 1563 Size: 2883 373 3063 Fragments arranged by size: 3063 2883 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2173 2387 2435 2717 3534 4382 5766 6034 Size: 541 214 48 282 817 848 1384 268 Cuts at: 6034 1632 Size: 1917 Fragments arranged by size: 1917 1384 848 817 541 282 268 214 48 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6122 AflIII A'CryG_T Cuts at: 1203 5218 6195 1203 Size: 4015 977 1327 Fragments arranged by size: 4015 1327 977 AgeI A'CCGG_T Cuts at: 5150 5150 Size: 6319 AhdI GACnn_n'nnGTC Cuts at: 5048 5048 Size: 6319 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6319 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6319 AlwNI CAG_nnn'CTG Cuts at: 3466 4062 5634 3466 Size: 596 1572 4151 Fragments arranged by size: 4151 1572 596 ApaLI G'TGCA_C Cuts at: 4376 5532 4376 Size: 1156 5163 ApoI r'AATT_y Cuts at: 1493 2472 2720 2765 3628 3854 4006 6115 1493 Size: 979 248 45 863 226 152 2109 1697 Fragments arranged by size: 2109 1697 979 863 248 226 152 45 AseI AT'TA_AT Cuts at: 160 1032 2388 4873 160 Size: 872 1356 2485 1606 Fragments arranged by size: 2485 1606 1356 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6319 AvaI C'yCGr_G Cuts at: 1870 1943 2877 3483 3644 1870 Size: 73 934 606 161 4545 Fragments arranged by size: 4545 934 606 161 73 BamHI G'GATC_C Cuts at: 3649 4016 3649 Size: 367 5952 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2134 3640 5095 618 Size: 325 217 627 347 1506 1455 1842 Fragments arranged by size: 1842 1506 1455 627 347 325 217 BanII G_rGCy'C Cuts at: 1391 2470 3638 1391 Size: 1079 1168 4072 Fragments arranged by size: 4072 1168 1079 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3598 928 Size: 971 1699 3649 Fragments arranged by size: 3649 1699 971 BbvCI CC'TCA_GC Cuts at: 3573 3573 Size: 6319 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4510 4544 1903 Size: 34 2573 34 3678 Fragments arranged by size: 3678 2573 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2876 3131 4211 5421 2876 Size: 255 1080 1210 3774 Fragments arranged by size: 3774 1210 1080 255 BclI T'GATC_A Cuts at: 1548 2892 1548 Size: 1344 4975 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4930 136 Size: 107 122 71 631 605 3258 1525 Fragments arranged by size: 3258 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2233 6314 2233 Size: 4081 2238 BlpI GC'TnA_GC Cuts at: 2088 3736 2088 Size: 1648 4671 Bme1580I G_kGCm'C Cuts at: 1790 2677 2937 3218 4380 5536 1790 Size: 887 260 281 1162 1156 2573 Fragments arranged by size: 2573 1162 1156 887 281 260 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6319 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4050 5008 442 Size: 416 53 83 399 24 2633 958 1753 Fragments arranged by size: 2633 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6319 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2581 2581 Size: 6319 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2309 4979 1239 Size: 1070 2670 2579 Fragments arranged by size: 2670 2579 1070 Bpu10I CC'TnA_GC Cuts at: 2255 3573 2255 Size: 1318 5001 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2231 4444 5309 5607 2231 Size: 2213 865 298 2943 Fragments arranged by size: 2943 2213 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4982 882 Size: 617 3483 2219 Fragments arranged by size: 3483 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2095 4110 493 Size: 711 891 2015 2702 Fragments arranged by size: 2702 2015 891 711 BsaBI GATnn'nnATC Cuts at: 6313 6313 Size: 6319 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4126 4508 5143 275 Size: 53 83 186 936 2593 382 635 1451 Fragments arranged by size: 2593 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4752 5150 5424 5571 5982 4752 Size: 398 274 147 411 5089 Fragments arranged by size: 5089 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2771 2801 1178 Size: 30 165 30 1368 30 4696 Fragments arranged by size: 4696 1368 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2447 2795 3323 1186 Size: 1261 348 528 4182 Fragments arranged by size: 4182 1261 528 348 BseYI C'CCAG_C Cuts at: 3382 5522 6018 3382 Size: 2140 496 3683 Fragments arranged by size: 3683 2140 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3025 1163 Size: 573 1289 4457 Fragments arranged by size: 4457 1289 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3769 4530 5558 1540 Size: 275 186 1768 761 1028 2301 Fragments arranged by size: 2301 1768 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2094 4380 4465 5536 2094 Size: 2286 85 1071 2877 Fragments arranged by size: 2877 2286 1071 85 BsmI GAATG_Cn' Cuts at: 3830 3923 3830 Size: 93 6226 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2094 2470 2677 2937 3218 3638 4380 Size: 399 304 376 207 260 281 420 742 Cuts at: 4380 4465 5536 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 420 399 376 304 281 260 207 85 BspEI T'CCGG_A Cuts at: 5982 5982 Size: 6319 BspHI T'CATG_A Cuts at: 4208 5938 4208 Size: 1730 4589 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2097 2604 3674 844 Size: 1253 507 1070 3489 Fragments arranged by size: 3489 1253 1070 507 BsrBI CCG'CTC Cuts at: 1394 1823 2153 4206 1394 Size: 429 330 2053 3507 Fragments arranged by size: 3507 2053 429 330 BsrDI GCAATG_nn' Cuts at: 65 1248 4814 4988 65 Size: 1183 3566 174 1396 Fragments arranged by size: 3566 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4963 5150 1700 Size: 110 3153 187 2869 Fragments arranged by size: 3153 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6319 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6319 BssSI C'ACGA_G Cuts at: 4379 5391 4379 Size: 1012 5307 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6319 BstBI TT'CG_AA Cuts at: 5123 5123 Size: 6319 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6319 BstYI r'GATC_y Cuts at: 1972 2233 3649 4016 4401 4418 5870 5956 5968 Size: 261 1416 367 385 17 1452 86 12 Cuts at: 5968 6314 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1416 385 367 346 261 86 17 12 BstZ17I GTA'TAC Cuts at: 3287 3287 Size: 6319 BtgI C'CryG_G Cuts at: 513 1065 2004 3130 513 Size: 552 939 1126 3702 Fragments arranged by size: 3702 1126 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2144 3490 3504 487 Size: 683 56 918 1346 14 3302 Fragments arranged by size: 3302 1346 918 683 56 14 BtsI GCAGTG_nn' Cuts at: 3835 4629 4649 3835 Size: 794 20 5505 Fragments arranged by size: 5505 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6319 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2983 3018 529 Size: 35 2419 35 3830 Fragments arranged by size: 3830 2419 35 35 DraI TTT'AAA Cuts at: 2176 3623 2176 Size: 1447 4872 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5326 809 Size: 507 4010 1802 Fragments arranged by size: 4010 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3390 3766 4655 Size: 54 1425 50 275 18 1560 376 889 Cuts at: 4655 6284 8 Size: 1629 43 Fragments arranged by size: 1629 1560 1425 889 376 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3766 1537 Size: 275 1954 4090 Fragments arranged by size: 4090 1954 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2014 2384 2618 3000 4249 1353 Size: 661 370 234 382 1249 3423 Fragments arranged by size: 3423 1249 661 382 370 234 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4906 5280 5426 387 Size: 1064 138 3317 374 146 1280 Fragments arranged by size: 3317 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2173 2309 2387 2435 2717 3534 4382 Size: 393 541 136 78 48 282 817 848 Cuts at: 4382 4979 5766 6034 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 848 817 787 597 541 393 282 268 136 78 48 EcoO109I rG'GnC_Cy Cuts at: 3172 3172 Size: 6319 EcoRI G'AATT_C Cuts at: 2720 3628 4006 2720 Size: 908 378 5033 Fragments arranged by size: 5033 908 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2203 2320 2203 Size: 117 6202 FspI TGC'GCA Cuts at: 4825 4825 Size: 6319 HaeII r_GCGC'y Cuts at: 1678 5466 1678 Size: 3788 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2225 2257 2819 2851 3176 Size: 32 135 32 653 32 562 32 325 Cuts at: 3176 3208 3323 3355 4960 4992 5034 5066 1373 Size: 32 115 32 1605 32 42 32 2626 Fragments arranged by size: 2626 1605 653 562 325 135 115 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3663 3909 669 Size: 988 281 1725 246 3079 Fragments arranged by size: 3079 1725 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6319 HpaI GTT'AAC Cuts at: 3909 3909 Size: 6319 KpnI G_GTAC'C Cuts at: 3644 3644 Size: 6319 MfeI C'AATT_G Cuts at: 3918 3918 Size: 6319 MluI A'CGCG_T Cuts at: 6195 6195 Size: 6319 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2322 2631 5433 5617 1305 Size: 1017 309 2802 184 2007 Fragments arranged by size: 2802 2007 1017 309 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3392 10 Size: 54 1425 343 1560 2937 Fragments arranged by size: 2937 1560 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2615 2976 4277 4636 4795 518 Size: 648 1449 361 1301 359 159 2042 Fragments arranged by size: 2042 1449 1301 648 361 359 159 MspA1I CmG'CkG Cuts at: 1606 3735 4412 5560 5805 1606 Size: 2129 677 1148 245 2120 Fragments arranged by size: 2129 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3130 513 Size: 552 939 1126 3702 Fragments arranged by size: 3702 1126 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6319 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6319 NotI GC'GGCC_GC Cuts at: 3766 3766 Size: 6319 NspI r_CATG'y Cuts at: 1132 1229 3677 5222 1132 Size: 97 2448 1545 2229 Fragments arranged by size: 2448 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6319 PciI A'CATG_T Cuts at: 5218 5218 Size: 6319 PflMI CCAn_nnn'nTGG Cuts at: 1108 3085 1108 Size: 1977 4342 PfoI T'CCnGG_A Cuts at: 2325 3360 2325 Size: 1035 5284 PmeI GTTT'AAAC Cuts at: 3623 3623 Size: 6319 PmlI CAC'GTG Cuts at: 2095 2095 Size: 6319 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4388 4420 5901 5933 4388 Size: 32 1481 32 4774 Fragments arranged by size: 4774 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6319 PsiI TTA'TAA Cuts at: 3889 3889 Size: 6319 PspXI vC'TCGA_Gb Cuts at: 3483 3483 Size: 6319 PstI C_TGCA'G Cuts at: 830 3430 3671 830 Size: 2600 241 3478 Fragments arranged by size: 3478 2600 241 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6319 SalI G'TCGA_C Cuts at: 1936 3661 1936 Size: 1725 4594 SapI GCTCTTCn'nnn_ Cuts at: 2014 2384 3000 2014 Size: 370 616 5333 Fragments arranged by size: 5333 616 370 SbfI CC_TGCA'GG Cuts at: 3671 3671 Size: 6319 ScaI AGT'ACT Cuts at: 4567 6279 4567 Size: 1712 4607 SexAI A'CCwGG_T Cuts at: 3437 3437 Size: 6319 SfcI C'TryA_G Cuts at: 826 1045 2482 3426 3667 4802 5483 5674 826 Size: 219 1437 944 241 1135 681 191 1471 Fragments arranged by size: 1471 1437 1135 944 681 241 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6319 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6319 SmaI CCC'GGG Cuts at: 1872 3646 1872 Size: 1774 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2246 3483 4423 5324 5586 820 Size: 197 926 303 1237 940 901 262 1553 Fragments arranged by size: 1553 1237 940 926 901 303 262 197 SnaBI TAC'GTA Cuts at: 493 4110 493 Size: 3617 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6319 SphI G_CATG'C Cuts at: 1132 3677 1132 Size: 2545 3774 SspI AAT'ATT Cuts at: 5 52 4243 5 Size: 47 4191 2081 Fragments arranged by size: 4191 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6319 StyI C'CwwG_G Cuts at: 513 1065 2004 3130 3758 513 Size: 552 939 1126 628 3074 Fragments arranged by size: 3074 1126 939 628 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4346 4363 4516 4701 5146 325 Size: 1255 2766 17 153 185 445 1498 Fragments arranged by size: 2766 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4565 6277 96 Size: 274 80 33 51 1443 2588 1712 138 Fragments arranged by size: 2588 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2771 2803 6279 6311 2771 Size: 32 3476 32 2779 Fragments arranged by size: 3476 2779 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6319 XbaI T'CTAG_A Cuts at: 3655 3655 Size: 6319 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2575 2575 Size: 6319 XhoI C'TCGA_G Cuts at: 1943 3483 1943 Size: 1540 4779 XmaI C'CCGG_G Cuts at: 1870 3644 1870 Size: 1774 4545 XmnI GAAnn'nnTTC Cuts at: 3009 4448 3009 Size: 1439 4880 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4127 276 Size: 53 83 186 936 2593 2468 Fragments arranged by size: 2593 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PstI PvuI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspOMI PsrI PvuII RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI