Restriction map for
pFN21AE1971
(Linear) (Six-base) MAPSORT of: KIEE1971.seq Check: 3303 from: 1 to: 1614
KIEE1971.orf
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AccI GT'mk_AC
Cuts at: 0 1281 1614
Size: 1281 333
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 168 382 430 712 1529 1614
Size: 168 214 48 282 817 85
Fragments arranged by size:
817 282 214 168 85 48
AlwNI CAG_nnn'CTG
Cuts at: 0 1461 1614
Size: 1461 153
ApoI r'AATT_y
Cuts at: 0 467 715 760 1614
Size: 467 248 45 854
Fragments arranged by size:
854 467 248 45
AseI AT'TA_AT
Cuts at: 0 383 1614
Size: 383 1231
AvaI C'yCGr_G
Cuts at: 0 872 1478 1614
Size: 872 606 136
Fragments arranged by size:
872 606 136
BanI G'GyrC_C
Cuts at: 0 129 1614
Size: 129 1485
BanII G_rGCy'C
Cuts at: 0 465 1614
Size: 465 1149
BbsI GAAGACnn'nnnn_
Cuts at: 0 1593 1614
Size: 1593 21
BbvCI CC'TCA_GC
Cuts at: 0 1568 1614
Size: 1568 46
BciVI GTATCCnnnnn_n'
Cuts at: 0 871 1126 1614
Size: 871 255 488
Fragments arranged by size:
871 488 255
BclI T'GATC_A
Cuts at: 0 887 1614
Size: 887 727
BglII A'GATC_T
Cuts at: 0 228 1614
Size: 228 1386
BlpI GC'TnA_GC
Cuts at: 0 83 1614
Size: 83 1531
Bme1580I G_kGCm'C
Cuts at: 0 672 932 1213 1614
Size: 672 260 281 401
Fragments arranged by size:
672 401 281 260
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 576 1614
Size: 576 1038
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 1614
Size: 304 1310
Bpu10I CC'TnA_GC
Cuts at: 0 250 1568 1614
Size: 250 1318 46
Fragments arranged by size:
1318 250 46
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 1614
Size: 226 1388
BsaAI yAC'GTr
Cuts at: 0 90 1614
Size: 90 1524
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 766 796 1614
Size: 766 30 818
Fragments arranged by size:
818 766 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 442 790 1318 1614
Size: 442 348 528 296
Fragments arranged by size:
528 442 348 296
BseYI C'CCAG_C
Cuts at: 0 1377 1614
Size: 1377 237
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1020 1614
Size: 1020 594
BsiHKAI G_wGCw'C
Cuts at: 0 89 1614
Size: 89 1525
Bsp1286I G_dGCh'C
Cuts at: 0 89 465 672 932 1213 1614
Size: 89 376 207 260 281 401
Fragments arranged by size:
401 376 281 260 207 89
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 92 599 1614
Size: 92 507 1015
Fragments arranged by size:
1015 507 92
BsrBI CCG'CTC
Cuts at: 0 148 1614
Size: 148 1466
BstYI r'GATC_y
Cuts at: 0 228 1614
Size: 228 1386
BstZ17I GTA'TAC
Cuts at: 0 1282 1614
Size: 1282 332
BtgI C'CryG_G
Cuts at: 0 1125 1614
Size: 1125 489
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 139 1485 1499 1614
Size: 139 1346 14 115
Fragments arranged by size:
1346 139 115 14
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 978 1013 1614
Size: 978 35 601
Fragments arranged by size:
978 601 35
DraI TTT'AAA
Cuts at: 0 171 1614
Size: 171 1443
EaeI y'GGCC_r
Cuts at: 0 1385 1614
Size: 1385 229
EarI CTCTTCn'nnn_
Cuts at: 0 9 379 613 995 1614
Size: 9 370 234 382 619
Fragments arranged by size:
619 382 370 234 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 168 304 382 430 712 1529 1614
Size: 168 136 78 48 282 817 85
Fragments arranged by size:
817 282 168 136 85 78 48
EcoO109I rG'GnC_Cy
Cuts at: 0 1167 1614
Size: 1167 447
EcoRI G'AATT_C
Cuts at: 0 715 1614
Size: 715 899
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 198 315 1614
Size: 198 117 1299
Fragments arranged by size:
1299 198 117
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 220 252 814 846 1171 1203 1318 1350
Size: 220 32 562 32 325 32 115 32
Cuts at: 1350 1614
Size: 264
Fragments arranged by size:
562 325 264 220 115 32 32 32
32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 317 626 1614
Size: 317 309 988
Fragments arranged by size:
988 317 309
MscI TGG'CCA
Cuts at: 0 1387 1614
Size: 1387 227
MslI CAynn'nnrTG
Cuts at: 0 610 971 1614
Size: 610 361 643
Fragments arranged by size:
643 610 361
NcoI C'CATG_G
Cuts at: 0 1125 1614
Size: 1125 489
PflMI CCAn_nnn'nTGG
Cuts at: 0 1080 1614
Size: 1080 534
PfoI T'CCnGG_A
Cuts at: 0 320 1355 1614
Size: 320 1035 259
Fragments arranged by size:
1035 320 259
PmlI CAC'GTG
Cuts at: 0 90 1614
Size: 90 1524
PspXI vC'TCGA_Gb
Cuts at: 0 1478 1614
Size: 1478 136
PstI C_TGCA'G
Cuts at: 0 1425 1614
Size: 1425 189
SapI GCTCTTCn'nnn_
Cuts at: 0 9 379 995 1614
Size: 9 370 616 619
Fragments arranged by size:
619 616 370 9
SexAI A'CCwGG_T
Cuts at: 0 1432 1614
Size: 1432 182
SfcI C'TryA_G
Cuts at: 0 477 1421 1614
Size: 477 944 193
Fragments arranged by size:
944 477 193
SmlI C'TyrA_G
Cuts at: 0 241 1478 1614
Size: 241 1237 136
Fragments arranged by size:
1237 241 136
StyI C'CwwG_G
Cuts at: 0 1125 1614
Size: 1125 489
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 766 798 1614
Size: 766 32 816
Fragments arranged by size:
816 766 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 570 1614
Size: 570 1044
XhoI C'TCGA_G
Cuts at: 0 1478 1614
Size: 1478 136
XmnI GAAnn'nnTTC
Cuts at: 0 1004 1614
Size: 1004 610
Enzymes that do cut:
AccI AcuI AlwNI ApoI AseI AvaI BanI BanII
BbsI BbvCI BciVI BclI BglII BlpI Bme1580I BplI
BpmI Bpu10I BpuEI BsaAI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspMI BsrBI BstYI BstZ17I BtgI BtgZI
CspCI DraI EaeI EarI Eco57MI EcoO109I EcoRI FalI
Hin4I MmeI MscI MslI NcoI PflMI PfoI PmlI
PspXI PstI SapI SexAI SfcI SmlI StyI TstI
XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AsiSI
AvrII BaeI BamHI BbeI BcgI BfrBI BglI BmgBI
BmrI BmtI BsaI BsaBI BsaHI BsaWI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI Bsu36I BtsI ClaI
DraIII DrdI EagI EciI EcoICRI EcoNI EcoRV FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI MspA1I NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PmeI
PpiI PpuMI PshAI PsiI PspOMI PsrI PvuI PvuII
RsrII SacI SacII SalI SanDI SbfI ScaI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII TatI Tth111I XbaI XmaI
ZraI