(Circular) (Six-base) MAPSORT of: pFN21AE5842.seq Check: 1890 from: 1 to: 10321 pFN21AE5842 10321 bp With 182 enzymes: SgfI * November 21, 2014 12:14 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 8131 278 Size: 53 83 186 936 6595 2468 Fragments arranged by size: 6595 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6770 7664 1937 Size: 4833 894 4594 Fragments arranged by size: 4833 4594 894 Acc65I G'GTAC_C Cuts at: 7642 7642 Size: 10321 AclI AA'CG_TT Cuts at: 1563 8448 8821 1563 Size: 6885 373 3063 Fragments arranged by size: 6885 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2533 2631 3093 3629 3957 4718 5265 5301 Size: 901 98 462 536 328 761 547 36 Cuts at: 5301 7182 7283 7438 8384 9768 10036 1632 Size: 1881 101 155 946 1384 268 1917 Fragments arranged by size: 1917 1881 1384 946 901 761 547 536 462 328 268 155 101 98 36 AfeI AGC'GCT Cuts at: 7517 7517 Size: 10321 AflII C'TTAA_G Cuts at: 820 1017 4595 5705 5732 820 Size: 197 3578 1110 27 5409 Fragments arranged by size: 5409 3578 1110 197 27 AflIII A'CryG_T Cuts at: 1203 3669 5322 9220 10197 1203 Size: 2466 1653 3898 977 1327 Fragments arranged by size: 3898 2466 1653 1327 977 AgeI A'CCGG_T Cuts at: 9152 9152 Size: 10321 AhdI GACnn_n'nnGTC Cuts at: 9050 9050 Size: 10321 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 10321 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2486 2673 3381 4677 4859 1184 Size: 1302 187 708 1296 182 6646 Fragments arranged by size: 6646 1302 1296 708 187 182 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6072 6104 6072 Size: 32 10289 AlwNI CAG_nnn'CTG Cuts at: 2573 2609 4283 8064 9636 2573 Size: 36 1674 3781 1572 3258 Fragments arranged by size: 3781 3258 1674 1572 36 ApaI G_GGCC'C Cuts at: 3831 5133 6927 3831 Size: 1302 1794 7225 Fragments arranged by size: 7225 1794 1302 ApaLI G'TGCA_C Cuts at: 3882 8378 9534 3882 Size: 4496 1156 4669 Fragments arranged by size: 4669 4496 1156 ApoI r'AATT_y Cuts at: 1493 2699 2963 3155 3540 3621 5030 5141 5615 Size: 1206 264 192 385 81 1409 111 474 Cuts at: 5615 5627 5834 6608 7630 7856 8008 10117 1493 Size: 12 207 774 1022 226 152 2109 1697 Fragments arranged by size: 2109 1697 1409 1206 1022 774 474 385 264 226 207 192 152 111 81 12 AseI AT'TA_AT Cuts at: 160 1032 8875 160 Size: 872 7843 1606 Fragments arranged by size: 7843 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10321 AvaI C'yCGr_G Cuts at: 1870 1943 2491 6327 6981 7646 1870 Size: 73 548 3836 654 665 4545 Fragments arranged by size: 4545 3836 665 654 548 73 BamHI G'GATC_C Cuts at: 7651 8018 7651 Size: 367 9954 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2279 3749 5807 6132 7642 Size: 325 217 627 492 1470 2058 325 1510 Cuts at: 7642 9097 618 Size: 1455 1842 Fragments arranged by size: 2058 1842 1510 1470 1455 627 492 325 325 217 BanII G_rGCy'C Cuts at: 1391 3831 5133 6927 7640 1391 Size: 2440 1302 1794 713 4072 Fragments arranged by size: 4072 2440 1794 1302 713 BbeI G_GCGC'C Cuts at: 6136 6136 Size: 10321 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2457 3734 4967 6465 928 Size: 971 558 1277 1233 1498 4784 Fragments arranged by size: 4784 1498 1277 1233 971 558 BbvCI CC'TCA_GC Cuts at: 3288 6936 3288 Size: 3648 6673 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3833 3867 8512 8546 1903 Size: 34 1896 34 4645 34 3678 Fragments arranged by size: 4645 3678 1896 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4470 8213 9423 4470 Size: 3743 1210 5368 Fragments arranged by size: 5368 3743 1210 BclI T'GATC_A Cuts at: 1548 2235 4293 1548 Size: 687 2058 7576 Fragments arranged by size: 7576 2058 687 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3306 8932 136 Size: 107 122 71 631 605 1634 5626 1525 Fragments arranged by size: 5626 1634 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6196 6408 10316 6196 Size: 212 3908 6201 Fragments arranged by size: 6201 3908 212 BlpI GC'TnA_GC Cuts at: 5172 5275 5416 7738 5172 Size: 103 141 2322 7755 Fragments arranged by size: 7755 2322 141 103 Bme1580I G_kGCm'C Cuts at: 1790 3831 3886 5133 6927 8382 9538 1790 Size: 2041 55 1247 1794 1455 1156 2573 Fragments arranged by size: 2573 2041 1794 1455 1247 1156 55 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 10321 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4866 6590 7029 Size: 416 53 83 399 24 3449 1724 439 Cuts at: 7029 8052 9010 442 Size: 1023 958 1753 Fragments arranged by size: 3449 1753 1724 1023 958 439 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 10321 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3583 7534 3583 Size: 3951 6370 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2118 2180 2892 3000 4161 4719 5142 6210 Size: 879 62 712 108 1161 558 423 1068 Cuts at: 6210 6371 7029 8981 1239 Size: 161 658 1952 2579 Fragments arranged by size: 2579 1952 1161 1068 879 712 658 558 423 161 108 62 Bpu10I CC'TnA_GC Cuts at: 3288 3468 6936 3288 Size: 180 3468 6673 Fragments arranged by size: 6673 3468 180 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3188 5483 6017 6866 7110 8446 9311 9609 3188 Size: 2295 534 849 244 1336 865 298 3900 Fragments arranged by size: 3900 2295 1336 865 849 534 298 244 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6650 8984 882 Size: 617 5151 2334 2219 Fragments arranged by size: 5151 2334 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5027 5360 6665 8112 493 Size: 711 3823 333 1305 1447 2702 Fragments arranged by size: 3823 2702 1447 1305 711 333 BsaBI GATnn'nnATC Cuts at: 4032 7086 10315 4032 Size: 3054 3229 4038 Fragments arranged by size: 4038 3229 3054 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6133 8128 8510 9145 Size: 53 83 186 936 4600 1995 382 635 Cuts at: 9145 275 Size: 1451 Fragments arranged by size: 4600 1995 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4530 5426 8754 9152 9426 9573 9984 4530 Size: 896 3328 398 274 147 411 4867 Fragments arranged by size: 4867 3328 896 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2825 2855 3563 3593 5894 Size: 30 165 30 1422 30 708 30 2301 Cuts at: 5894 5924 6276 6306 1178 Size: 30 352 30 5193 Fragments arranged by size: 5193 2301 1422 708 352 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2466 5918 5921 7430 7499 7514 7517 7577 Size: 1280 3452 3 1509 69 15 3 60 Cuts at: 7577 1186 Size: 3930 Fragments arranged by size: 3930 3452 1509 1280 69 60 15 3 3 BseYI C'CCAG_C Cuts at: 2224 2639 3061 3441 5070 7187 7592 9524 10020 Size: 415 422 380 1629 2117 405 1932 496 Cuts at: 10020 2224 Size: 2525 Fragments arranged by size: 2525 2117 1932 1629 496 422 415 405 380 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 9748 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 7771 8532 9560 1540 Size: 275 186 5770 761 1028 2301 Fragments arranged by size: 5770 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2779 2846 3886 4147 4255 8382 8467 9538 2779 Size: 67 1040 261 108 4127 85 1071 3562 Fragments arranged by size: 4127 3562 1071 1040 261 108 85 67 BsmI GAATG_Cn' Cuts at: 3340 3775 5403 7832 7925 3340 Size: 435 1628 2429 93 5736 Fragments arranged by size: 5736 2429 1628 435 93 BsmBI CGTCTCn'nnnn_ Cuts at: 7350 7350 Size: 10321 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2779 2846 3831 3886 4147 4255 5133 Size: 399 989 67 985 55 261 108 878 Cuts at: 5133 6927 7640 8382 8467 9538 1391 Size: 1794 713 742 85 1071 2174 Fragments arranged by size: 2174 1794 1071 989 985 878 742 713 399 261 108 85 67 55 BspEI T'CCGG_A Cuts at: 4530 5426 9984 4530 Size: 896 4558 4867 Fragments arranged by size: 4867 4558 896 BspHI T'CATG_A Cuts at: 8210 9940 8210 Size: 1730 8591 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4072 7045 7676 844 Size: 3228 2973 631 3489 Fragments arranged by size: 3489 3228 2973 631 BsrBI CCG'CTC Cuts at: 1394 1823 5380 8208 1394 Size: 429 3557 2828 3507 Fragments arranged by size: 3557 3507 2828 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4818 7587 8816 8990 65 Size: 1183 3570 2769 1229 174 1396 Fragments arranged by size: 3570 2769 1396 1229 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6129 8965 9152 1700 Size: 110 4319 2836 187 2869 Fragments arranged by size: 4319 2869 2836 187 110 BsrGI T'GTAC_A Cuts at: 96 7114 96 Size: 7018 3303 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10321 BssSI C'ACGA_G Cuts at: 8381 9393 8381 Size: 1012 9309 BstAPI GCAn_nnn'nTGC Cuts at: 794 5807 794 Size: 5013 5308 BstBI TT'CG_AA Cuts at: 5623 9125 5623 Size: 3502 6819 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 10321 BstXI CCAn_nnnn'nTGG Cuts at: 2766 2978 3823 6579 2766 Size: 212 845 2756 6508 Fragments arranged by size: 6508 2756 845 212 BstYI r'GATC_y Cuts at: 1972 2826 3424 3691 4135 4867 6196 6408 6427 Size: 854 598 267 444 732 1329 212 19 Cuts at: 6427 7262 7651 8018 8403 8420 9872 9958 9970 Size: 835 389 367 385 17 1452 86 12 Cuts at: 9970 10316 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1329 854 835 732 598 444 389 385 367 346 267 212 86 19 17 12 BstZ17I GTA'TAC Cuts at: 6771 6771 Size: 10321 Bsu36I CC'TnA_GG Cuts at: 2586 4008 5043 2586 Size: 1422 1035 7864 Fragments arranged by size: 7864 1422 1035 BtgI C'CryG_G Cuts at: 513 1065 2004 3695 4469 4872 5149 7375 513 Size: 552 939 1691 774 403 277 2226 3459 Fragments arranged by size: 3459 2226 1691 939 774 552 403 277 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3353 3836 487 Size: 683 56 2127 483 6972 Fragments arranged by size: 6972 2127 683 483 56 BtsI GCAGTG_nn' Cuts at: 2049 2921 3088 4364 4661 4788 7837 8631 8651 Size: 872 167 1276 297 127 3049 794 20 Cuts at: 8651 2049 Size: 3719 Fragments arranged by size: 3719 3049 1276 872 794 297 167 127 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 10321 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 10286 DraI TTT'AAA Cuts at: 7625 7625 Size: 10321 DraIII CAC_nnn'GTG Cuts at: 4878 4878 Size: 10321 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6160 6545 6762 9328 809 Size: 507 4844 385 217 2566 1802 Fragments arranged by size: 4844 2566 1802 507 385 217 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4213 4351 4466 Size: 54 1425 50 275 18 2383 138 115 Cuts at: 4466 6272 6581 6732 7768 8657 10286 8 Size: 1806 309 151 1036 889 1629 43 Fragments arranged by size: 2383 1806 1629 1425 1036 889 309 275 151 138 115 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 7768 1537 Size: 275 5956 4090 Fragments arranged by size: 5956 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2814 3552 3608 4529 4620 5043 5482 5520 Size: 1461 738 56 921 91 423 439 38 Cuts at: 5520 5652 6327 7095 7503 8251 1353 Size: 132 675 768 408 748 3423 Fragments arranged by size: 3423 1461 921 768 748 738 675 439 423 408 132 91 56 38 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2785 5692 8908 9282 9428 387 Size: 1064 138 1196 2907 3216 374 146 1280 Fragments arranged by size: 3216 2907 1280 1196 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2118 2180 2533 2631 2892 3000 3093 Size: 393 486 62 353 98 261 108 93 Cuts at: 3093 3629 3957 4161 4718 4719 5142 5265 5301 Size: 536 328 204 557 1 423 123 36 Cuts at: 5301 6210 6371 7029 7182 7283 7438 8384 8981 Size: 909 161 658 153 101 155 946 597 Cuts at: 8981 9768 10036 1239 Size: 787 268 1524 Fragments arranged by size: 1524 946 909 787 658 597 557 536 486 423 393 353 328 268 261 204 161 155 153 123 108 101 98 93 62 36 1 EcoNI CCTnn'n_nnAGG Cuts at: 7142 7553 7142 Size: 411 9910 EcoO109I rG'GnC_Cy Cuts at: 2009 2582 3827 3899 4012 4812 5129 5738 6923 Size: 573 1245 72 113 800 317 609 1185 Cuts at: 6923 6924 2009 Size: 1 5406 Fragments arranged by size: 5406 1245 1185 800 609 573 317 113 72 1 EcoRI G'AATT_C Cuts at: 3621 5615 7630 8008 3621 Size: 1994 2015 378 5934 Fragments arranged by size: 5934 2015 1994 378 EcoRV GAT'ATC Cuts at: 3244 3244 Size: 10321 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2215 2215 Size: 10321 FspI TGC'GCA Cuts at: 4001 5093 5591 8827 4001 Size: 1092 498 3236 5495 Fragments arranged by size: 5495 3236 1092 498 FspAI rTGC'GCAy Cuts at: 4001 5093 5591 4001 Size: 1092 498 8731 Fragments arranged by size: 8731 1092 498 HaeII r_GCGC'y Cuts at: 1678 6136 7519 9468 1678 Size: 4458 1383 1949 2531 Fragments arranged by size: 4458 2531 1949 1383 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2461 2493 2555 2569 2587 Size: 32 135 32 889 32 62 14 18 Cuts at: 2587 2601 2932 2964 3271 3303 3496 3528 5258 Size: 14 331 32 307 32 193 32 1730 Cuts at: 5258 5290 5481 5513 6625 6657 7247 7279 7415 Size: 32 191 32 1112 32 590 32 136 Cuts at: 7415 7447 7568 7595 7600 7627 8962 8994 9036 Size: 32 121 27 5 27 1335 32 42 Cuts at: 9036 9068 1373 Size: 32 2626 Fragments arranged by size: 2626 1730 1335 1112 889 590 331 307 193 191 136 135 121 62 42 32 32 32 32 32 32 32 32 32 32 32 32 32 27 27 18 14 14 5 HincII GTy'rAC Cuts at: 669 1657 1938 5103 7665 7911 669 Size: 988 281 3165 2562 246 3079 Fragments arranged by size: 3165 3079 2562 988 281 246 HindIII A'AGCT_T Cuts at: 748 3969 5729 7450 748 Size: 3221 1760 1721 3619 Fragments arranged by size: 3619 3221 1760 1721 HpaI GTT'AAC Cuts at: 7911 7911 Size: 10321 KasI G'GCGC_C Cuts at: 6132 6132 Size: 10321 KpnI G_GTAC'C Cuts at: 7646 7646 Size: 10321 MfeI C'AATT_G Cuts at: 4217 7920 4217 Size: 3703 6618 MluI A'CGCG_T Cuts at: 10197 10197 Size: 10321 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3468 3937 5892 5898 6058 9435 9619 1305 Size: 2163 469 1955 6 160 3377 184 2007 Fragments arranged by size: 3377 2163 2007 1955 469 184 160 6 MscI TGG'CCA Cuts at: 10 64 1489 1832 4215 4353 6583 6734 10 Size: 54 1425 343 2383 138 2230 151 3597 Fragments arranged by size: 3597 2383 2230 1425 343 151 138 54 MslI CAynn'nnrTG Cuts at: 518 1166 2942 3821 4571 5442 7313 8279 8638 Size: 648 1776 879 750 871 1871 966 359 Cuts at: 8638 8797 518 Size: 159 2042 Fragments arranged by size: 2042 1871 1776 966 879 871 750 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2096 2158 3226 5070 5279 5570 7231 7737 Size: 490 62 1068 1844 209 291 1661 506 Cuts at: 7737 8414 9562 9807 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1844 1661 1148 1068 677 506 490 291 245 209 62 NarI GG'CG_CC Cuts at: 6133 6133 Size: 10321 NcoI C'CATG_G Cuts at: 513 1065 2004 3695 7375 513 Size: 552 939 1691 3680 3459 Fragments arranged by size: 3680 3459 1691 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 10321 NheI G'CTAG_C Cuts at: 1052 1052 Size: 10321 NotI GC'GGCC_GC Cuts at: 7768 7768 Size: 10321 NspI r_CATG'y Cuts at: 1132 1229 3673 5326 5805 7297 7679 9224 1132 Size: 97 2444 1653 479 1492 382 1545 2229 Fragments arranged by size: 2444 2229 1653 1545 1492 479 382 97 PasI CC'CwG_GG Cuts at: 1348 6920 1348 Size: 5572 4749 PciI A'CATG_T Cuts at: 3669 5322 9220 3669 Size: 1653 3898 4770 Fragments arranged by size: 4770 3898 1653 PflMI CCAn_nnn'nTGG Cuts at: 1108 2639 2836 7381 1108 Size: 1531 197 4545 4048 Fragments arranged by size: 4545 4048 1531 197 PfoI T'CCnGG_A Cuts at: 2829 4138 2829 Size: 1309 9012 PmeI GTTT'AAAC Cuts at: 7625 7625 Size: 10321 PmlI CAC'GTG Cuts at: 5360 5360 Size: 10321 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 8390 8422 9903 9935 8390 Size: 32 1481 32 8776 Fragments arranged by size: 8776 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2009 2582 3899 4012 5738 2009 Size: 573 1317 113 1726 6592 Fragments arranged by size: 6592 1726 1317 573 113 PshAI GACnn'nnGTC Cuts at: 1613 4989 1613 Size: 3376 6945 PsiI TTA'TAA Cuts at: 4116 7891 4116 Size: 3775 6546 PspOMI G'GGCC_C Cuts at: 3827 5129 6923 3827 Size: 1302 1794 7225 Fragments arranged by size: 7225 1794 1302 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2873 2905 6788 6820 2873 Size: 32 3883 32 6374 Fragments arranged by size: 6374 3883 32 32 PstI C_TGCA'G Cuts at: 830 4687 7673 830 Size: 3857 2986 3478 Fragments arranged by size: 3857 3478 2986 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10321 PvuII CAG'CTG Cuts at: 2158 3226 5279 5570 7231 2158 Size: 1068 2053 291 1661 5248 Fragments arranged by size: 5248 2053 1661 1068 291 SalI G'TCGA_C Cuts at: 1936 7663 1936 Size: 5727 4594 SanDI GG'GwC_CC Cuts at: 2009 2009 Size: 10321 SapI GCTCTTCn'nnn_ Cuts at: 2814 7503 2814 Size: 4689 5632 SbfI CC_TGCA'GG Cuts at: 7673 7673 Size: 10321 ScaI AGT'ACT Cuts at: 3872 8569 10281 3872 Size: 4697 1712 3912 Fragments arranged by size: 4697 3912 1712 SfcI C'TryA_G Cuts at: 826 1045 4424 4683 5219 5390 7669 8804 9485 Size: 219 3379 259 536 171 2279 1135 681 Cuts at: 9485 9676 826 Size: 191 1471 Fragments arranged by size: 3379 2279 1471 1135 681 536 259 219 191 171 SfoI GGC'GCC Cuts at: 6134 6134 Size: 10321 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10321 SgrAI Cr'CCGG_yG Cuts at: 1700 6129 1700 Size: 4429 5892 SmaI CCC'GGG Cuts at: 1872 2493 7648 1872 Size: 621 5155 4545 Fragments arranged by size: 5155 4545 621 SmlI C'TyrA_G Cuts at: 820 1017 1943 3167 4595 5498 5705 5732 6032 Size: 197 926 1224 1428 903 207 27 300 Cuts at: 6032 6845 6981 7125 8425 9326 9588 820 Size: 813 136 144 1300 901 262 1553 Fragments arranged by size: 1553 1428 1300 1224 926 903 901 813 300 262 207 197 144 136 27 SnaBI TAC'GTA Cuts at: 493 5027 6665 8112 493 Size: 4534 1638 1447 2702 Fragments arranged by size: 4534 2702 1638 1447 SpeI A'CTAG_T Cuts at: 152 152 Size: 10321 SphI G_CATG'C Cuts at: 1132 7297 7679 1132 Size: 6165 382 3774 Fragments arranged by size: 6165 3774 382 SspI AAT'ATT Cuts at: 5 52 3766 8245 5 Size: 47 3714 4479 2081 Fragments arranged by size: 4479 3714 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 10321 StyI C'CwwG_G Cuts at: 513 1065 2004 2283 3392 3582 3695 4796 7375 Size: 552 939 279 1109 190 113 1101 2579 Cuts at: 7375 7760 513 Size: 385 3074 Fragments arranged by size: 3074 2579 1109 1101 939 552 385 279 190 113 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3053 3833 4579 6566 6609 7454 8348 Size: 1255 1473 780 746 1987 43 845 894 Cuts at: 8348 8365 8518 8703 9148 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1987 1498 1473 1255 894 845 780 746 445 185 153 43 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2312 3870 6860 Size: 274 80 33 51 1443 335 1558 2990 Cuts at: 6860 7114 8567 10279 96 Size: 254 1453 1712 138 Fragments arranged by size: 2990 1712 1558 1453 1443 335 274 254 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2263 2295 2825 2857 10281 10313 2263 Size: 32 530 32 7424 32 2271 Fragments arranged by size: 7424 2271 530 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 5493 6157 1316 Size: 4177 664 5480 Fragments arranged by size: 5480 4177 664 XbaI T'CTAG_A Cuts at: 3140 7657 3140 Size: 4517 5804 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3013 5974 7589 3013 Size: 2961 1615 5745 Fragments arranged by size: 5745 2961 1615 XhoI C'TCGA_G Cuts at: 1943 6981 1943 Size: 5038 5283 XmaI C'CCGG_G Cuts at: 1870 2491 7646 1870 Size: 621 5155 4545 Fragments arranged by size: 5155 4545 621 XmnI GAAnn'nnTTC Cuts at: 2691 5619 8450 2691 Size: 2928 2831 4562 Fragments arranged by size: 4562 2928 2831 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 8129 276 Size: 53 83 186 936 6595 2468 Fragments arranged by size: 6595 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BfrBI BsiWI EcoICRI FseI NaeI NgoMIV NruI NsiI PacI PspXI RsrII SacI SacII SexAI SfiI SrfI SwaI