Restriction map for pFN21AE5842
 (Linear) (Six-base) MAPSORT of: KIEE5842.seq  Check: 1556  from: 1  to: 5616



KIEE5842.orf

 With 182 enzymes: SgfI * 

                        November 21, 2014 11:14  ..

AccI GT'mk_AC

Cuts at:      0    4765    5616 
   Size:       4765     851
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     528     626    1088    1624    1952    2713    3260    3296 
   Size:        528      98     462     536     328     761     547      36

Cuts at:   3296    5177    5278    5433    5616 
   Size:       1881     101     155     183

  Fragments arranged by size: 

                1881     761     547     536     528     462     328     183
                 155     101      98      36
 
AfeI AGC'GCT

Cuts at:      0    5512    5616 
   Size:       5512     104
 
AflII C'TTAA_G

Cuts at:      0    2590    3700    3727    5616 
   Size:       2590    1110      27    1889

  Fragments arranged by size: 

                2590    1889    1110      27
 
AflIII A'CryG_T

Cuts at:      0    1664    3317    5616 
   Size:       1664    1653    2299

  Fragments arranged by size: 

                2299    1664    1653
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     481     668    1376    2672    2854    5616 
   Size:        481     187     708    1296     182    2762

  Fragments arranged by size: 

                2762    1296     708     481     187     182
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    4067    4099    5616 
   Size:       4067      32    1517

  Fragments arranged by size: 

                4067    1517      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     568     604    2278    5616 
   Size:        568      36    1674    3338

  Fragments arranged by size: 

                3338    1674     568      36
 
ApaI G_GGCC'C

Cuts at:      0    1826    3128    4922    5616 
   Size:       1826    1302    1794     694

  Fragments arranged by size: 

                1826    1794    1302     694
 
ApaLI G'TGCA_C

Cuts at:      0    1877    5616 
   Size:       1877    3739
 
ApoI r'AATT_y

Cuts at:      0     694     958    1150    1535    1616    3025    3136    3610 
   Size:        694     264     192     385      81    1409     111     474

Cuts at:   3610    3622    3829    4603    5616 
   Size:         12     207     774    1013

  Fragments arranged by size: 

                1409    1013     774     694     474     385     264     207
                 192     111      81      12
 
AvaI C'yCGr_G

Cuts at:      0     486    4322    4976    5616 
   Size:        486    3836     654     640

  Fragments arranged by size: 

                3836     654     640     486
 
BanI G'GyrC_C

Cuts at:      0     274    1744    3802    4127    5616 
   Size:        274    1470    2058     325    1489

  Fragments arranged by size: 

                2058    1489    1470     325     274
 
BanII G_rGCy'C

Cuts at:      0    1826    3128    4922    5616 
   Size:       1826    1302    1794     694

  Fragments arranged by size: 

                1826    1794    1302     694
 
BbeI G_GCGC'C

Cuts at:      0    4131    5616 
   Size:       4131    1485
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     452    1729    2962    4460    5616 
   Size:        452    1277    1233    1498    1156

  Fragments arranged by size: 

                1498    1277    1233    1156     452
 
BbvCI CC'TCA_GC

Cuts at:      0    1283    4931    5616 
   Size:       1283    3648     685

  Fragments arranged by size: 

                3648    1283     685
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1828    1862    5616 
   Size:       1828      34    3754

  Fragments arranged by size: 

                3754    1828      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2465    5616 
   Size:       2465    3151
 
BclI T'GATC_A

Cuts at:      0     230    2288    5616 
   Size:        230    2058    3328

  Fragments arranged by size: 

                3328    2058     230
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1301    5616 
   Size:       1301    4315
 
BglII A'GATC_T

Cuts at:      0    4191    4403    5616 
   Size:       4191     212    1213

  Fragments arranged by size: 

                4191    1213     212
 
BlpI GC'TnA_GC

Cuts at:      0    3167    3270    3411    5616 
   Size:       3167     103     141    2205

  Fragments arranged by size: 

                3167    2205     141     103
 
Bme1580I G_kGCm'C

Cuts at:      0    1826    1881    3128    4922    5616 
   Size:       1826      55    1247    1794     694

  Fragments arranged by size: 

                1826    1794    1247     694      55
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2861    4585    5024    5616 
   Size:       2861    1724     439     592

  Fragments arranged by size: 

                2861    1724     592     439
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1578    5529    5616 
   Size:       1578    3951      87

  Fragments arranged by size: 

                3951    1578      87
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     113     175     887     995    2156    2714    3137    4205 
   Size:        113      62     712     108    1161     558     423    1068

Cuts at:   4205    4366    5024    5616 
   Size:        161     658     592

  Fragments arranged by size: 

                1161    1068     712     658     592     558     423     161
                 113     108      62
 
Bpu10I CC'TnA_GC

Cuts at:      0    1283    1463    4931    5616 
   Size:       1283     180    3468     685

  Fragments arranged by size: 

                3468    1283     685     180
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1183    3478    4012    4861    5105    5616 
   Size:       1183    2295     534     849     244     511

  Fragments arranged by size: 

                2295    1183     849     534     511     244
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    4645    5616 
   Size:       4645     971
 
BsaAI yAC'GTr

Cuts at:      0    3022    3355    4660    5616 
   Size:       3022     333    1305     956

  Fragments arranged by size: 

                3022    1305     956     333
 
BsaBI GATnn'nnATC

Cuts at:      0    2027    5081    5616 
   Size:       2027    3054     535

  Fragments arranged by size: 

                3054    2027     535
 
BsaHI Gr'CG_yC

Cuts at:      0    4128    5616 
   Size:       4128    1488
 
BsaWI w'CCGG_w

Cuts at:      0    2525    3421    5616 
   Size:       2525     896    2195

  Fragments arranged by size: 

                2525    2195     896
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     820     850    1558    1588    3889    3919    4271    4301 
   Size:        820      30     708      30    2301      30     352      30

Cuts at:   4301    5616 
   Size:       1315

  Fragments arranged by size: 

                2301    1315     820     708     352      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     461    3913    3916    5425    5494    5509    5512    5572 
   Size:        461    3452       3    1509      69      15       3      60

Cuts at:   5572    5616 
   Size:         44

  Fragments arranged by size: 

                3452    1509     461      69      60      44      15       3
                   3
 
BseYI C'CCAG_C

Cuts at:      0     219     634    1056    1436    3065    5182    5587    5616 
   Size:        219     415     422     380    1629    2117     405      29

  Fragments arranged by size: 

                2117    1629     422     415     405     380     219      29
            
 
BsiHKAI G_wGCw'C

Cuts at:      0     774     841    1881    2142    2250    5616 
   Size:        774      67    1040     261     108    3366

  Fragments arranged by size: 

                3366    1040     774     261     108      67
 
BsmI GAATG_Cn'

Cuts at:      0    1335    1770    3398    5616 
   Size:       1335     435    1628    2218

  Fragments arranged by size: 

                2218    1628    1335     435
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    5345    5616 
   Size:       5345     271
 
Bsp1286I G_dGCh'C

Cuts at:      0     774     841    1826    1881    2142    2250    3128    4922 
   Size:        774      67     985      55     261     108     878    1794

Cuts at:   4922    5616 
   Size:        694

  Fragments arranged by size: 

                1794     985     878     774     694     261     108      67
                  55
 
BspEI T'CCGG_A

Cuts at:      0    2525    3421    5616 
   Size:       2525     896    2195

  Fragments arranged by size: 

                2525    2195     896
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    2067    5040    5616 
   Size:       2067    2973     576

  Fragments arranged by size: 

                2973    2067     576
 
BsrBI CCG'CTC

Cuts at:      0    3375    5616 
   Size:       3375    2241
 
BsrDI GCAATG_nn'

Cuts at:      0    2813    5582    5616 
   Size:       2813    2769      34

  Fragments arranged by size: 

                2813    2769      34
 
BsrFI r'CCGG_y

Cuts at:      0    4124    5616 
   Size:       4124    1492
 
BsrGI T'GTAC_A

Cuts at:      0    5109    5616 
   Size:       5109     507
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    3802    5616 
   Size:       3802    1814
 
BstBI TT'CG_AA

Cuts at:      0    3618    5616 
   Size:       3618    1998
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     761     973    1818    4574    5616 
   Size:        761     212     845    2756    1042

  Fragments arranged by size: 

                2756    1042     845     761     212
 
BstYI r'GATC_y

Cuts at:      0     821    1419    1686    2130    2862    4191    4403    4422 
   Size:        821     598     267     444     732    1329     212      19

Cuts at:   4422    5257    5616 
   Size:        835     359

  Fragments arranged by size: 

                1329     835     821     732     598     444     359     267
                 212      19
 
BstZ17I GTA'TAC

Cuts at:      0    4766    5616 
   Size:       4766     850
 
Bsu36I CC'TnA_GG

Cuts at:      0     581    2003    3038    5616 
   Size:        581    1422    1035    2578

  Fragments arranged by size: 

                2578    1422    1035     581
 
BtgI C'CryG_G

Cuts at:      0    1690    2464    2867    3144    5370    5616 
   Size:       1690     774     403     277    2226     246

  Fragments arranged by size: 

                2226    1690     774     403     277     246
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1348    1831    5616 
   Size:       1348     483    3785

  Fragments arranged by size: 

                3785    1348     483
 
BtsI GCAGTG_nn'

Cuts at:      0      44     916    1083    2359    2656    2783    5616 
   Size:         44     872     167    1276     297     127    2833

  Fragments arranged by size: 

                2833    1276     872     297     167     127      44
 
DraIII CAC_nnn'GTG

Cuts at:      0    2873    5616 
   Size:       2873    2743
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    4155    4540    4757    5616 
   Size:       4155     385     217     859

  Fragments arranged by size: 

                4155     859     385     217
 
EaeI y'GGCC_r

Cuts at:      0    2208    2346    2461    4267    4576    4727    5616 
   Size:       2208     138     115    1806     309     151     889

  Fragments arranged by size: 

                2208    1806     889     309     151     138     115
 
EarI CTCTTCn'nnn_

Cuts at:      0     809    1547    1603    2524    2615    3038    3477    3515 
   Size:        809     738      56     921      91     423     439      38

Cuts at:   3515    3647    4322    5090    5498    5616 
   Size:        132     675     768     408     118

  Fragments arranged by size: 

                 921     809     768     738     675     439     423     408
                 132     118      91      56      38
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     780    3687    5616 
   Size:        780    2907    1929

  Fragments arranged by size: 

                2907    1929     780
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     113     175     528     626     887     995    1088    1624 
   Size:        113      62     353      98     261     108      93     536

Cuts at:   1624    1952    2156    2713    2714    3137    3260    3296    4205 
   Size:        328     204     557       1     423     123      36     909

Cuts at:   4205    4366    5024    5177    5278    5433    5616 
   Size:        161     658     153     101     155     183

  Fragments arranged by size: 

                 909     658     557     536     423     353     328     261
                 204     183     161     155     153     123     113     108
                 101      98      93      62      36       1
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    5137    5548    5616 
   Size:       5137     411      68

  Fragments arranged by size: 

                5137     411      68
 
EcoO109I rG'GnC_Cy

Cuts at:      0       4     577    1822    1894    2007    2807    3124    3733 
   Size:          4     573    1245      72     113     800     317     609

Cuts at:   3733    4918    4919    5616 
   Size:       1185       1     697

  Fragments arranged by size: 

                1245    1185     800     697     609     573     317     113
                  72       4       1
 
EcoRI G'AATT_C

Cuts at:      0    1616    3610    5616 
   Size:       1616    1994    2006

  Fragments arranged by size: 

                2006    1994    1616
 
EcoRV GAT'ATC

Cuts at:      0    1239    5616 
   Size:       1239    4377
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     210    5616 
   Size:        210    5406
 
FspI TGC'GCA

Cuts at:      0    1996    3088    3586    5616 
   Size:       1996    1092     498    2030

  Fragments arranged by size: 

                2030    1996    1092     498
 
FspAI rTGC'GCAy

Cuts at:      0    1996    3088    3586    5616 
   Size:       1996    1092     498    2030

  Fragments arranged by size: 

                2030    1996    1092     498
 
HaeII r_GCGC'y

Cuts at:      0    4131    5514    5616 
   Size:       4131    1383     102

  Fragments arranged by size: 

                4131    1383     102
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     456     488     550     564     582     596     927     959 
   Size:        456      32      62      14      18      14     331      32

Cuts at:    959    1266    1298    1491    1523    3253    3285    3476    3508 
   Size:        307      32     193      32    1730      32     191      32

Cuts at:   3508    4620    4652    5242    5274    5410    5442    5563    5590 
   Size:       1112      32     590      32     136      32     121      27

Cuts at:   5590    5595    5616 
   Size:          5      21

  Fragments arranged by size: 

                1730    1112     590     456     331     307     193     191
                 136     121      62      32      32      32      32      32
                  32      32      32      32      27      21      18      14
                  14       5
 
HincII GTy'rAC

Cuts at:      0    3098    5616 
   Size:       3098    2518
 
HindIII A'AGCT_T

Cuts at:      0    1964    3724    5445    5616 
   Size:       1964    1760    1721     171

  Fragments arranged by size: 

                1964    1760    1721     171
 
KasI G'GCGC_C

Cuts at:      0    4127    5616 
   Size:       4127    1489
 
MfeI C'AATT_G

Cuts at:      0    2212    5616 
   Size:       2212    3404
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1463    1932    3887    3893    4053    5616 
   Size:       1463     469    1955       6     160    1563

  Fragments arranged by size: 

                1955    1563    1463     469     160       6
 
MscI TGG'CCA

Cuts at:      0    2210    2348    4578    4729    5616 
   Size:       2210     138    2230     151     887

  Fragments arranged by size: 

                2230    2210     887     151     138
 
MslI CAynn'nnrTG

Cuts at:      0     937    1816    2566    3437    5308    5616 
   Size:        937     879     750     871    1871     308

  Fragments arranged by size: 

                1871     937     879     871     750     308
 
MspA1I CmG'CkG

Cuts at:      0      91     153    1221    3065    3274    3565    5226    5616 
   Size:         91      62    1068    1844     209     291    1661     390

  Fragments arranged by size: 

                1844    1661    1068     390     291     209      91      62
            
 
NarI GG'CG_CC

Cuts at:      0    4128    5616 
   Size:       4128    1488
 
NcoI C'CATG_G

Cuts at:      0    1690    5370    5616 
   Size:       1690    3680     246

  Fragments arranged by size: 

                3680    1690     246
 
NspI r_CATG'y

Cuts at:      0    1668    3321    3800    5292    5616 
   Size:       1668    1653     479    1492     324

  Fragments arranged by size: 

                1668    1653    1492     479     324
 
PasI CC'CwG_GG

Cuts at:      0    4915    5616 
   Size:       4915     701
 
PciI A'CATG_T

Cuts at:      0    1664    3317    5616 
   Size:       1664    1653    2299

  Fragments arranged by size: 

                2299    1664    1653
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     634     831    5376    5616 
   Size:        634     197    4545     240

  Fragments arranged by size: 

                4545     634     240     197
 
PfoI T'CCnGG_A

Cuts at:      0     824    2133    5616 
   Size:        824    1309    3483

  Fragments arranged by size: 

                3483    1309     824
 
PmlI CAC'GTG

Cuts at:      0    3355    5616 
   Size:       3355    2261
 
PpuMI rG'GwC_Cy

Cuts at:      0       4     577    1894    2007    3733    5616 
   Size:          4     573    1317     113    1726    1883

  Fragments arranged by size: 

                1883    1726    1317     573     113       4
 
PshAI GACnn'nnGTC

Cuts at:      0    2984    5616 
   Size:       2984    2632
 
PsiI TTA'TAA

Cuts at:      0    2111    5616 
   Size:       2111    3505
 
PspOMI G'GGCC_C

Cuts at:      0    1822    3124    4918    5616 
   Size:       1822    1302    1794     698

  Fragments arranged by size: 

                1822    1794    1302     698
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     868     900    4783    4815    5616 
   Size:        868      32    3883      32     801

  Fragments arranged by size: 

                3883     868     801      32      32
 
PstI C_TGCA'G

Cuts at:      0    2682    5616 
   Size:       2682    2934
 
PvuII CAG'CTG

Cuts at:      0     153    1221    3274    3565    5226    5616 
   Size:        153    1068    2053     291    1661     390

  Fragments arranged by size: 

                2053    1661    1068     390     291     153
 
SanDI GG'GwC_CC

Cuts at:      0       4    5616 
   Size:          4    5612
 
SapI GCTCTTCn'nnn_

Cuts at:      0     809    5498    5616 
   Size:        809    4689     118

  Fragments arranged by size: 

                4689     809     118
 
ScaI AGT'ACT

Cuts at:      0    1867    5616 
   Size:       1867    3749
 
SfcI C'TryA_G

Cuts at:      0    2419    2678    3214    3385    5616 
   Size:       2419     259     536     171    2231

  Fragments arranged by size: 

                2419    2231     536     259     171
 
SfoI GGC'GCC

Cuts at:      0    4129    5616 
   Size:       4129    1487
 
SgrAI Cr'CCGG_yG

Cuts at:      0    4124    5616 
   Size:       4124    1492
 
SmaI CCC'GGG

Cuts at:      0     488    5616 
   Size:        488    5128
 
SmlI C'TyrA_G

Cuts at:      0    1162    2590    3493    3700    3727    4027    4840    4976 
   Size:       1162    1428     903     207      27     300     813     136

Cuts at:   4976    5120    5616 
   Size:        144     496

  Fragments arranged by size: 

                1428    1162     903     813     496     300     207     144
                 136      27
 
SnaBI TAC'GTA

Cuts at:      0    3022    4660    5616 
   Size:       3022    1638     956

  Fragments arranged by size: 

                3022    1638     956
 
SphI G_CATG'C

Cuts at:      0    5292    5616 
   Size:       5292     324
 
SspI AAT'ATT

Cuts at:      0    1761    5616 
   Size:       1761    3855
 
StyI C'CwwG_G

Cuts at:      0     278    1387    1577    1690    2791    5370    5616 
   Size:        278    1109     190     113    1101    2579     246

  Fragments arranged by size: 

                2579    1109    1101     278     246     190     113
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1048    1828    2574    4561    4604    5449    5616 
   Size:       1048     780     746    1987      43     845     167

  Fragments arranged by size: 

                1987    1048     845     780     746     167      43
 
TatI w'GTAC_w

Cuts at:      0     307    1865    4855    5109    5616 
   Size:        307    1558    2990     254     507

  Fragments arranged by size: 

                2990    1558     507     307     254
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     258     290     820     852    5616 
   Size:        258      32     530      32    4764

  Fragments arranged by size: 

                4764     530     258      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    3488    4152    5616 
   Size:       3488     664    1464

  Fragments arranged by size: 

                3488    1464     664
 
XbaI T'CTAG_A

Cuts at:      0    1135    5616 
   Size:       1135    4481
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1008    3969    5584    5616 
   Size:       1008    2961    1615      32

  Fragments arranged by size: 

                2961    1615    1008      32
 
XhoI C'TCGA_G

Cuts at:      0    4976    5616 
   Size:       4976     640
 
XmaI C'CCGG_G

Cuts at:      0     486    5616 
   Size:        486    5130
 
XmnI GAAnn'nnTTC

Cuts at:      0     686    3614    5616 
   Size:        686    2928    2002

  Fragments arranged by size: 

                2928    2002     686
 

 Enzymes that do cut:

     AccI     AcuI     AfeI    AflII   AflIII     AlfI     AloI    AlwNI
     ApaI    ApaLI     ApoI     AvaI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BstAPI
    BstBI    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
   DraIII     DrdI     EaeI     EarI     EciI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     KasI     MfeI     MmeI     MscI     MslI   MspA1I     NarI
     NcoI     NspI     PasI     PciI    PflMI     PfoI     PmlI    PpuMI
    PshAI     PsiI   PspOMI     PsrI     PstI    PvuII    SanDI     SapI
     ScaI     SfcI     SfoI    SgrAI     SmaI     SmlI    SnaBI     SphI
     SspI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AgeI     AhdI     AleI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI    BfrBI    BmgBI     BmtI
     BsgI    BsiEI    BsiWI    BspHI   BssHII    BssSI   BstEII     ClaI
    CspCI     DraI     EagI  EcoICRI     FseI     HpaI     KpnI     MluI
     NaeI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PmeI     PpiI    PspXI     PvuI    RsrII     SacI    SacII     SalI
     SbfI    SexAI     SfiI     SgfI     SpeI     SrfI     StuI     SwaI
     ZraI
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