(Linear) (Six-base) MAPSORT of: KIEE5842.seq Check: 1556 from: 1 to: 5616 KIEE5842.orf With 182 enzymes: SgfI * November 21, 2014 11:14 .. AccI GT'mk_AC Cuts at: 0 4765 5616 Size: 4765 851 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 528 626 1088 1624 1952 2713 3260 3296 Size: 528 98 462 536 328 761 547 36 Cuts at: 3296 5177 5278 5433 5616 Size: 1881 101 155 183 Fragments arranged by size: 1881 761 547 536 528 462 328 183 155 101 98 36 AfeI AGC'GCT Cuts at: 0 5512 5616 Size: 5512 104 AflII C'TTAA_G Cuts at: 0 2590 3700 3727 5616 Size: 2590 1110 27 1889 Fragments arranged by size: 2590 1889 1110 27 AflIII A'CryG_T Cuts at: 0 1664 3317 5616 Size: 1664 1653 2299 Fragments arranged by size: 2299 1664 1653 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 481 668 1376 2672 2854 5616 Size: 481 187 708 1296 182 2762 Fragments arranged by size: 2762 1296 708 481 187 182 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 4067 4099 5616 Size: 4067 32 1517 Fragments arranged by size: 4067 1517 32 AlwNI CAG_nnn'CTG Cuts at: 0 568 604 2278 5616 Size: 568 36 1674 3338 Fragments arranged by size: 3338 1674 568 36 ApaI G_GGCC'C Cuts at: 0 1826 3128 4922 5616 Size: 1826 1302 1794 694 Fragments arranged by size: 1826 1794 1302 694 ApaLI G'TGCA_C Cuts at: 0 1877 5616 Size: 1877 3739 ApoI r'AATT_y Cuts at: 0 694 958 1150 1535 1616 3025 3136 3610 Size: 694 264 192 385 81 1409 111 474 Cuts at: 3610 3622 3829 4603 5616 Size: 12 207 774 1013 Fragments arranged by size: 1409 1013 774 694 474 385 264 207 192 111 81 12 AvaI C'yCGr_G Cuts at: 0 486 4322 4976 5616 Size: 486 3836 654 640 Fragments arranged by size: 3836 654 640 486 BanI G'GyrC_C Cuts at: 0 274 1744 3802 4127 5616 Size: 274 1470 2058 325 1489 Fragments arranged by size: 2058 1489 1470 325 274 BanII G_rGCy'C Cuts at: 0 1826 3128 4922 5616 Size: 1826 1302 1794 694 Fragments arranged by size: 1826 1794 1302 694 BbeI G_GCGC'C Cuts at: 0 4131 5616 Size: 4131 1485 BbsI GAAGACnn'nnnn_ Cuts at: 0 452 1729 2962 4460 5616 Size: 452 1277 1233 1498 1156 Fragments arranged by size: 1498 1277 1233 1156 452 BbvCI CC'TCA_GC Cuts at: 0 1283 4931 5616 Size: 1283 3648 685 Fragments arranged by size: 3648 1283 685 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1828 1862 5616 Size: 1828 34 3754 Fragments arranged by size: 3754 1828 34 BciVI GTATCCnnnnn_n' Cuts at: 0 2465 5616 Size: 2465 3151 BclI T'GATC_A Cuts at: 0 230 2288 5616 Size: 230 2058 3328 Fragments arranged by size: 3328 2058 230 BglI GCCn_nnn'nGGC Cuts at: 0 1301 5616 Size: 1301 4315 BglII A'GATC_T Cuts at: 0 4191 4403 5616 Size: 4191 212 1213 Fragments arranged by size: 4191 1213 212 BlpI GC'TnA_GC Cuts at: 0 3167 3270 3411 5616 Size: 3167 103 141 2205 Fragments arranged by size: 3167 2205 141 103 Bme1580I G_kGCm'C Cuts at: 0 1826 1881 3128 4922 5616 Size: 1826 55 1247 1794 694 Fragments arranged by size: 1826 1794 1247 694 55 BmrI ACTGGGnnnn_n' Cuts at: 0 2861 4585 5024 5616 Size: 2861 1724 439 592 Fragments arranged by size: 2861 1724 592 439 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1578 5529 5616 Size: 1578 3951 87 Fragments arranged by size: 3951 1578 87 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 113 175 887 995 2156 2714 3137 4205 Size: 113 62 712 108 1161 558 423 1068 Cuts at: 4205 4366 5024 5616 Size: 161 658 592 Fragments arranged by size: 1161 1068 712 658 592 558 423 161 113 108 62 Bpu10I CC'TnA_GC Cuts at: 0 1283 1463 4931 5616 Size: 1283 180 3468 685 Fragments arranged by size: 3468 1283 685 180 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1183 3478 4012 4861 5105 5616 Size: 1183 2295 534 849 244 511 Fragments arranged by size: 2295 1183 849 534 511 244 BsaI GGTCTCn'nnnn_ Cuts at: 0 4645 5616 Size: 4645 971 BsaAI yAC'GTr Cuts at: 0 3022 3355 4660 5616 Size: 3022 333 1305 956 Fragments arranged by size: 3022 1305 956 333 BsaBI GATnn'nnATC Cuts at: 0 2027 5081 5616 Size: 2027 3054 535 Fragments arranged by size: 3054 2027 535 BsaHI Gr'CG_yC Cuts at: 0 4128 5616 Size: 4128 1488 BsaWI w'CCGG_w Cuts at: 0 2525 3421 5616 Size: 2525 896 2195 Fragments arranged by size: 2525 2195 896 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 820 850 1558 1588 3889 3919 4271 4301 Size: 820 30 708 30 2301 30 352 30 Cuts at: 4301 5616 Size: 1315 Fragments arranged by size: 2301 1315 820 708 352 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 461 3913 3916 5425 5494 5509 5512 5572 Size: 461 3452 3 1509 69 15 3 60 Cuts at: 5572 5616 Size: 44 Fragments arranged by size: 3452 1509 461 69 60 44 15 3 3 BseYI C'CCAG_C Cuts at: 0 219 634 1056 1436 3065 5182 5587 5616 Size: 219 415 422 380 1629 2117 405 29 Fragments arranged by size: 2117 1629 422 415 405 380 219 29 BsiHKAI G_wGCw'C Cuts at: 0 774 841 1881 2142 2250 5616 Size: 774 67 1040 261 108 3366 Fragments arranged by size: 3366 1040 774 261 108 67 BsmI GAATG_Cn' Cuts at: 0 1335 1770 3398 5616 Size: 1335 435 1628 2218 Fragments arranged by size: 2218 1628 1335 435 BsmBI CGTCTCn'nnnn_ Cuts at: 0 5345 5616 Size: 5345 271 Bsp1286I G_dGCh'C Cuts at: 0 774 841 1826 1881 2142 2250 3128 4922 Size: 774 67 985 55 261 108 878 1794 Cuts at: 4922 5616 Size: 694 Fragments arranged by size: 1794 985 878 774 694 261 108 67 55 BspEI T'CCGG_A Cuts at: 0 2525 3421 5616 Size: 2525 896 2195 Fragments arranged by size: 2525 2195 896 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 2067 5040 5616 Size: 2067 2973 576 Fragments arranged by size: 2973 2067 576 BsrBI CCG'CTC Cuts at: 0 3375 5616 Size: 3375 2241 BsrDI GCAATG_nn' Cuts at: 0 2813 5582 5616 Size: 2813 2769 34 Fragments arranged by size: 2813 2769 34 BsrFI r'CCGG_y Cuts at: 0 4124 5616 Size: 4124 1492 BsrGI T'GTAC_A Cuts at: 0 5109 5616 Size: 5109 507 BstAPI GCAn_nnn'nTGC Cuts at: 0 3802 5616 Size: 3802 1814 BstBI TT'CG_AA Cuts at: 0 3618 5616 Size: 3618 1998 BstXI CCAn_nnnn'nTGG Cuts at: 0 761 973 1818 4574 5616 Size: 761 212 845 2756 1042 Fragments arranged by size: 2756 1042 845 761 212 BstYI r'GATC_y Cuts at: 0 821 1419 1686 2130 2862 4191 4403 4422 Size: 821 598 267 444 732 1329 212 19 Cuts at: 4422 5257 5616 Size: 835 359 Fragments arranged by size: 1329 835 821 732 598 444 359 267 212 19 BstZ17I GTA'TAC Cuts at: 0 4766 5616 Size: 4766 850 Bsu36I CC'TnA_GG Cuts at: 0 581 2003 3038 5616 Size: 581 1422 1035 2578 Fragments arranged by size: 2578 1422 1035 581 BtgI C'CryG_G Cuts at: 0 1690 2464 2867 3144 5370 5616 Size: 1690 774 403 277 2226 246 Fragments arranged by size: 2226 1690 774 403 277 246 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1348 1831 5616 Size: 1348 483 3785 Fragments arranged by size: 3785 1348 483 BtsI GCAGTG_nn' Cuts at: 0 44 916 1083 2359 2656 2783 5616 Size: 44 872 167 1276 297 127 2833 Fragments arranged by size: 2833 1276 872 297 167 127 44 DraIII CAC_nnn'GTG Cuts at: 0 2873 5616 Size: 2873 2743 DrdI GACnn_nn'nnGTC Cuts at: 0 4155 4540 4757 5616 Size: 4155 385 217 859 Fragments arranged by size: 4155 859 385 217 EaeI y'GGCC_r Cuts at: 0 2208 2346 2461 4267 4576 4727 5616 Size: 2208 138 115 1806 309 151 889 Fragments arranged by size: 2208 1806 889 309 151 138 115 EarI CTCTTCn'nnn_ Cuts at: 0 809 1547 1603 2524 2615 3038 3477 3515 Size: 809 738 56 921 91 423 439 38 Cuts at: 3515 3647 4322 5090 5498 5616 Size: 132 675 768 408 118 Fragments arranged by size: 921 809 768 738 675 439 423 408 132 118 91 56 38 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 780 3687 5616 Size: 780 2907 1929 Fragments arranged by size: 2907 1929 780 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 113 175 528 626 887 995 1088 1624 Size: 113 62 353 98 261 108 93 536 Cuts at: 1624 1952 2156 2713 2714 3137 3260 3296 4205 Size: 328 204 557 1 423 123 36 909 Cuts at: 4205 4366 5024 5177 5278 5433 5616 Size: 161 658 153 101 155 183 Fragments arranged by size: 909 658 557 536 423 353 328 261 204 183 161 155 153 123 113 108 101 98 93 62 36 1 EcoNI CCTnn'n_nnAGG Cuts at: 0 5137 5548 5616 Size: 5137 411 68 Fragments arranged by size: 5137 411 68 EcoO109I rG'GnC_Cy Cuts at: 0 4 577 1822 1894 2007 2807 3124 3733 Size: 4 573 1245 72 113 800 317 609 Cuts at: 3733 4918 4919 5616 Size: 1185 1 697 Fragments arranged by size: 1245 1185 800 697 609 573 317 113 72 4 1 EcoRI G'AATT_C Cuts at: 0 1616 3610 5616 Size: 1616 1994 2006 Fragments arranged by size: 2006 1994 1616 EcoRV GAT'ATC Cuts at: 0 1239 5616 Size: 1239 4377 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 210 5616 Size: 210 5406 FspI TGC'GCA Cuts at: 0 1996 3088 3586 5616 Size: 1996 1092 498 2030 Fragments arranged by size: 2030 1996 1092 498 FspAI rTGC'GCAy Cuts at: 0 1996 3088 3586 5616 Size: 1996 1092 498 2030 Fragments arranged by size: 2030 1996 1092 498 HaeII r_GCGC'y Cuts at: 0 4131 5514 5616 Size: 4131 1383 102 Fragments arranged by size: 4131 1383 102 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 456 488 550 564 582 596 927 959 Size: 456 32 62 14 18 14 331 32 Cuts at: 959 1266 1298 1491 1523 3253 3285 3476 3508 Size: 307 32 193 32 1730 32 191 32 Cuts at: 3508 4620 4652 5242 5274 5410 5442 5563 5590 Size: 1112 32 590 32 136 32 121 27 Cuts at: 5590 5595 5616 Size: 5 21 Fragments arranged by size: 1730 1112 590 456 331 307 193 191 136 121 62 32 32 32 32 32 32 32 32 32 27 21 18 14 14 5 HincII GTy'rAC Cuts at: 0 3098 5616 Size: 3098 2518 HindIII A'AGCT_T Cuts at: 0 1964 3724 5445 5616 Size: 1964 1760 1721 171 Fragments arranged by size: 1964 1760 1721 171 KasI G'GCGC_C Cuts at: 0 4127 5616 Size: 4127 1489 MfeI C'AATT_G Cuts at: 0 2212 5616 Size: 2212 3404 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1463 1932 3887 3893 4053 5616 Size: 1463 469 1955 6 160 1563 Fragments arranged by size: 1955 1563 1463 469 160 6 MscI TGG'CCA Cuts at: 0 2210 2348 4578 4729 5616 Size: 2210 138 2230 151 887 Fragments arranged by size: 2230 2210 887 151 138 MslI CAynn'nnrTG Cuts at: 0 937 1816 2566 3437 5308 5616 Size: 937 879 750 871 1871 308 Fragments arranged by size: 1871 937 879 871 750 308 MspA1I CmG'CkG Cuts at: 0 91 153 1221 3065 3274 3565 5226 5616 Size: 91 62 1068 1844 209 291 1661 390 Fragments arranged by size: 1844 1661 1068 390 291 209 91 62 NarI GG'CG_CC Cuts at: 0 4128 5616 Size: 4128 1488 NcoI C'CATG_G Cuts at: 0 1690 5370 5616 Size: 1690 3680 246 Fragments arranged by size: 3680 1690 246 NspI r_CATG'y Cuts at: 0 1668 3321 3800 5292 5616 Size: 1668 1653 479 1492 324 Fragments arranged by size: 1668 1653 1492 479 324 PasI CC'CwG_GG Cuts at: 0 4915 5616 Size: 4915 701 PciI A'CATG_T Cuts at: 0 1664 3317 5616 Size: 1664 1653 2299 Fragments arranged by size: 2299 1664 1653 PflMI CCAn_nnn'nTGG Cuts at: 0 634 831 5376 5616 Size: 634 197 4545 240 Fragments arranged by size: 4545 634 240 197 PfoI T'CCnGG_A Cuts at: 0 824 2133 5616 Size: 824 1309 3483 Fragments arranged by size: 3483 1309 824 PmlI CAC'GTG Cuts at: 0 3355 5616 Size: 3355 2261 PpuMI rG'GwC_Cy Cuts at: 0 4 577 1894 2007 3733 5616 Size: 4 573 1317 113 1726 1883 Fragments arranged by size: 1883 1726 1317 573 113 4 PshAI GACnn'nnGTC Cuts at: 0 2984 5616 Size: 2984 2632 PsiI TTA'TAA Cuts at: 0 2111 5616 Size: 2111 3505 PspOMI G'GGCC_C Cuts at: 0 1822 3124 4918 5616 Size: 1822 1302 1794 698 Fragments arranged by size: 1822 1794 1302 698 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 868 900 4783 4815 5616 Size: 868 32 3883 32 801 Fragments arranged by size: 3883 868 801 32 32 PstI C_TGCA'G Cuts at: 0 2682 5616 Size: 2682 2934 PvuII CAG'CTG Cuts at: 0 153 1221 3274 3565 5226 5616 Size: 153 1068 2053 291 1661 390 Fragments arranged by size: 2053 1661 1068 390 291 153 SanDI GG'GwC_CC Cuts at: 0 4 5616 Size: 4 5612 SapI GCTCTTCn'nnn_ Cuts at: 0 809 5498 5616 Size: 809 4689 118 Fragments arranged by size: 4689 809 118 ScaI AGT'ACT Cuts at: 0 1867 5616 Size: 1867 3749 SfcI C'TryA_G Cuts at: 0 2419 2678 3214 3385 5616 Size: 2419 259 536 171 2231 Fragments arranged by size: 2419 2231 536 259 171 SfoI GGC'GCC Cuts at: 0 4129 5616 Size: 4129 1487 SgrAI Cr'CCGG_yG Cuts at: 0 4124 5616 Size: 4124 1492 SmaI CCC'GGG Cuts at: 0 488 5616 Size: 488 5128 SmlI C'TyrA_G Cuts at: 0 1162 2590 3493 3700 3727 4027 4840 4976 Size: 1162 1428 903 207 27 300 813 136 Cuts at: 4976 5120 5616 Size: 144 496 Fragments arranged by size: 1428 1162 903 813 496 300 207 144 136 27 SnaBI TAC'GTA Cuts at: 0 3022 4660 5616 Size: 3022 1638 956 Fragments arranged by size: 3022 1638 956 SphI G_CATG'C Cuts at: 0 5292 5616 Size: 5292 324 SspI AAT'ATT Cuts at: 0 1761 5616 Size: 1761 3855 StyI C'CwwG_G Cuts at: 0 278 1387 1577 1690 2791 5370 5616 Size: 278 1109 190 113 1101 2579 246 Fragments arranged by size: 2579 1109 1101 278 246 190 113 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1048 1828 2574 4561 4604 5449 5616 Size: 1048 780 746 1987 43 845 167 Fragments arranged by size: 1987 1048 845 780 746 167 43 TatI w'GTAC_w Cuts at: 0 307 1865 4855 5109 5616 Size: 307 1558 2990 254 507 Fragments arranged by size: 2990 1558 507 307 254 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 258 290 820 852 5616 Size: 258 32 530 32 4764 Fragments arranged by size: 4764 530 258 32 32 Tth111I GACn'n_nGTC Cuts at: 0 3488 4152 5616 Size: 3488 664 1464 Fragments arranged by size: 3488 1464 664 XbaI T'CTAG_A Cuts at: 0 1135 5616 Size: 1135 4481 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1008 3969 5584 5616 Size: 1008 2961 1615 32 Fragments arranged by size: 2961 1615 1008 32 XhoI C'TCGA_G Cuts at: 0 4976 5616 Size: 4976 640 XmaI C'CCGG_G Cuts at: 0 486 5616 Size: 486 5130 XmnI GAAnn'nnTTC Cuts at: 0 686 3614 5616 Size: 686 2928 2002 Fragments arranged by size: 2928 2002 686 Enzymes that do cut: AccI AcuI AfeI AflII AflIII AlfI AloI AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraIII DrdI EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII HindIII KasI MfeI MmeI MscI MslI MspA1I NarI NcoI NspI PasI PciI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PsrI PstI PvuII SanDI SapI ScaI SfcI SfoI SgrAI SmaI SmlI SnaBI SphI SspI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AgeI AhdI AleI AscI AseI AsiSI AvrII BaeI BamHI BfrBI BmgBI BmtI BsgI BsiEI BsiWI BspHI BssHII BssSI BstEII ClaI CspCI DraI EagI EcoICRI FseI HpaI KpnI MluI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PmeI PpiI PspXI PvuI RsrII SacI SacII SalI SbfI SexAI SfiI SgfI SpeI SrfI StuI SwaI ZraI