################################## # NO CUT ENZYMES ( 69 enzymes) # ################################## AatII Acc65I AccII AcyI AgeI Alw44I ApaI ApaLI AscI Asp718I AviII BbeI BbrPI BsaBI BsaHI BsiEI BsiWI BspDI BsrBI BssHII BssSI BstUI BstXI ClaI DraIII DrdI EagI EclXI Eco52I EcoRI FseI FspI HgaI Hin1I KasI KpnI KspI MamI MfeI MluI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PaeR7I PinAI PmaCI PmeI PmlI PvuI SacI SacII SbfI SexAI SfiI SfoI SgrAI SmaI Sse8387I SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AccIII 1587 TCCGGA T^CCGGA AflII 1306 CTTAAG C^TTAAG AflIII 4610 ACATGT A^C[GA][CT]GT AhdI 2452 GACCTTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 8722 CCTGAGG CC^T[ACGT]AGG AspEI 2452 GACCTTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 5470 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 6507 CCTAGG C^CTAGG BfrI 1306 CTTAAG C^TTAAG BlnI 6507 CCTAGG C^CTAGG BsaAI 8280 TACGTA [CT]AC^GT[GA] BseAI 1587 TCCGGA T^CCGGA BsmBI 5636 GAGACG CGTCTC(1/5) BspEI 1587 TCCGGA T^CCGGA BspLU11I 4610 ACATGT A^CATGT BsrFI 2183 GCCGGT [GA]^CCGG[CT] Bsu36I 8722 CCTGAGG CC^T[ACGT]AGG Cfr10I 2183 GCCGGT [GA]^CCGG[CT] CpoI 8033 CGGTCCG CG^G[AT]CCG Eam1105I 2452 GACCTTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 8722 CCTGAGG CC^T[ACGT]AGG EcoRV 6148 GATATC GAT^ATC FauI 7313 CCCGC CCCGC(4/6) HpaI 2008 GTTAAC GTT^AAC MroI 1587 TCCGGA T^CCGGA PflFI 5470 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 7692 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RsrII 8033 CGGTCCG CG^G[AT]CCG SnaBI 8280 TACGTA TAC^GTA SphI 4281 GCATGC GCATG^C Tth111I 5470 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 6423 TCTAGA T^CTAGA XcmI 1827 CCACTTGAACGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AclI 1834 AACGTT AA^CGTT 2230 AACGTT AlwNI 2057 CAGTATCTG CAG[ACGT][ACGT][ACGT]^CTG 7697 CAGTCTCTG Aor51HI 4544 AGCGCT AGC^GCT 8519 AGCGCT AvaI 3935 CCCGAG C^[CT]CG[GA]G 7304 CTCGGG BanI 4451 GGCACC G^G[CT][GA]CC 8214 GGTGCC BbvCI 3777 CCTCAGC CCTCAGC(-5/-2) 6439 GCTGAGG BcgI 4014 GCACTACATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 7242 GCAGACAGCTCG BciVI 6395 GGATAC GTATCC(6/5) 4209 GTATCC BglI 2676 GCCTAAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 6365 GCCCATGTGGC BlpI 999 GCTAAGC GC^T[ACGT]AGC 3720 GCTAAGC Bpu1102I 999 GCTAAGC GC^T[ACGT]AGC 3720 GCTAAGC BsoBI 3935 CCCGAG C^[CT]CG[GA]G 7304 CTCGGG Bsp1407I 434 TGTACA T^GTACA 8324 TGTACA BsrGI 434 TGTACA T^GTACA 8324 TGTACA Bst1107I 6051 GTATAC GTA^TAC 6700 GTATAC BstAPI 2056 GCAGTATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 5904 GCAGCCTGTGC BstEII 2693 GGTCACC G^GT[ACGT]ACC 8140 GGTCACC BstPI 2693 GGTCACC G^GT[ACGT]ACC 8140 GGTCACC BstZ17I 6051 GTATAC GTA^TAC 6700 GTATAC CelII 999 GCTAAGC GC^T[ACGT]AGC 3720 GCTAAGC DraII 140 AGGACCT [GA]G^G[ACGT]CC[CT] 8254 GGGACCT DsaI 5186 CCATGG C^C[GA][CT]GG 6890 CCATGG Eco47III 4544 AGCGCT AGC^GCT 8519 AGCGCT EcoO109I 140 AGGACCT [GA]G^G[ACGT]CC[CT] 8254 GGGACCT EcoO65I 2693 GGTCACC G^GT[ACGT]ACC 8140 GGTCACC EcoT22I 1497 ATGCAT ATGCA^T 4279 ATGCAT NcoI 5186 CCATGG C^CATGG 6890 CCATGG NsiI 1497 ATGCAT ATGCA^T 4279 ATGCAT PflMI 4138 CCATCTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 6885 CCAGCCCATGG PpuMI 140 AGGACCT [GA]G^G[AT]CC[CT] 8254 GGGACCT Psp1406I 1834 AACGTT AA^CGTT 2230 AACGTT SalI 323 GTCGAC G^TCGAC 5552 GTCGAC SspBI 434 TGTACA T^GTACA 8324 TGTACA SspI 7144 AATATT AAT^ATT 7291 AATATT Van91I 4138 CCATCTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 6885 CCAGCCCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 121 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI ApoI AseI AsnI Asp700I AspHI AvaII BalI BamHI BanII BbsI BbvI BclI BcnI BfaI BglII BmyI BpmI BpuAI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspHI BspMI BsrDI BsrI BssKI BstBI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI EaeI EarI EcoNI EcoRII EcoT14I FbaI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI NspV PleI PshBI PstI PvuII RcaI RsaI SapI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SfuI SmlI SpeI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI