(Circular) (Six-base) MAPSORT of: pFN21AE3830.seq Check: 8346 from: 1 to: 13597 pFN21AE3830 13597 bp With 182 enzymes: SgfI * March 28, 2012 12:08 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2765 2765 Size: 13597 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 11407 278 Size: 53 83 186 936 9871 2468 Fragments arranged by size: 9871 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2329 7558 8057 8232 8706 9604 9942 10940 Size: 392 5229 499 175 474 898 338 998 Cuts at: 10940 1937 Size: 4594 Fragments arranged by size: 5229 4594 998 898 499 474 392 338 175 Acc65I G'GTAC_C Cuts at: 10918 10918 Size: 13597 AclI AA'CG_TT Cuts at: 1563 3840 4236 11724 12097 1563 Size: 2277 396 7488 373 3063 Fragments arranged by size: 7488 3063 2277 396 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2502 3990 4814 6672 7562 8975 11660 13044 Size: 870 1488 824 1858 890 1413 2685 1384 Cuts at: 13044 13312 1632 Size: 268 1917 Fragments arranged by size: 2685 1917 1858 1488 1413 1384 890 870 824 268 AfeI AGC'GCT Cuts at: 6551 10526 6551 Size: 3975 9622 AflII C'TTAA_G Cuts at: 820 1017 3311 820 Size: 197 2294 11106 Fragments arranged by size: 11106 2294 197 AflIII A'CryG_T Cuts at: 1203 6615 12496 13473 1203 Size: 5412 5881 977 1327 Fragments arranged by size: 5881 5412 1327 977 AgeI A'CCGG_T Cuts at: 12428 12428 Size: 13597 AhdI GACnn_n'nnGTC Cuts at: 4462 12326 4462 Size: 7864 5733 AleI CACnn'nnGTG Cuts at: 1166 5526 9141 1166 Size: 4360 3615 5622 Fragments arranged by size: 5622 4360 3615 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 10868 1184 Size: 9684 3913 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6657 6689 6657 Size: 32 13565 AlwNI CAG_nnn'CTG Cuts at: 4067 9707 11340 12912 4067 Size: 5640 1633 1572 4752 Fragments arranged by size: 5640 4752 1633 1572 ApaLI G'TGCA_C Cuts at: 11654 12810 11654 Size: 1156 12441 ApoI r'AATT_y Cuts at: 1493 2254 3786 5509 5651 5904 8217 9342 10906 Size: 761 1532 1723 142 253 2313 1125 1564 Cuts at: 10906 11132 11284 13393 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2313 2109 1723 1697 1564 1532 1125 761 253 226 152 142 AseI AT'TA_AT Cuts at: 160 1032 2571 2955 3468 4937 12151 160 Size: 872 1539 384 513 1469 7214 1606 Fragments arranged by size: 7214 1606 1539 1469 872 513 384 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 13597 AvaI C'yCGr_G Cuts at: 1870 1943 5940 9309 10922 1870 Size: 73 3997 3369 1613 4545 Fragments arranged by size: 4545 3997 3369 1613 73 AvrII C'CTAG_G Cuts at: 8512 8512 Size: 13597 BamHI G'GATC_C Cuts at: 2605 4834 5854 10927 11294 2605 Size: 2229 1020 5073 367 4908 Fragments arranged by size: 5073 4908 2229 1020 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 6456 10219 10918 12373 618 Size: 325 217 627 4669 3763 699 1455 1842 Fragments arranged by size: 4669 3763 1842 1455 699 627 325 217 BanII G_rGCy'C Cuts at: 1391 5363 7191 8481 10916 1391 Size: 3972 1828 1290 2435 4072 Fragments arranged by size: 4072 3972 2435 1828 1290 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2489 2691 4979 5328 7056 9214 928 Size: 971 590 202 2288 349 1728 2158 5311 Fragments arranged by size: 5311 2288 2158 1728 971 590 349 202 BbvCI CC'TCA_GC Cuts at: 5783 8445 5783 Size: 2662 10935 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6008 6042 9236 9270 11788 11822 1903 Size: 34 4071 34 3194 34 2518 34 3678 Fragments arranged by size: 4071 3678 3194 2518 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6225 8394 11489 12699 6225 Size: 2169 3095 1210 7123 Fragments arranged by size: 7123 3095 2169 1210 BclI T'GATC_A Cuts at: 1548 2763 3252 10423 1548 Size: 1215 489 7171 4722 Fragments arranged by size: 7171 4722 1215 489 BfrBI ATG'CAT Cuts at: 3504 6286 3504 Size: 2782 10815 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4687 8376 12208 Size: 107 122 71 631 605 3015 3689 3832 Cuts at: 12208 136 Size: 1525 Fragments arranged by size: 3832 3689 3015 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5944 9330 9447 9463 10767 13592 5944 Size: 3386 117 16 1304 2825 5949 Fragments arranged by size: 5949 3386 2825 1304 117 16 BlpI GC'TnA_GC Cuts at: 3005 5726 11014 3005 Size: 2721 5288 5588 Fragments arranged by size: 5588 5288 2721 Bme1580I G_kGCm'C Cuts at: 1790 11658 12814 1790 Size: 9868 1156 2573 Fragments arranged by size: 9868 2573 1156 BmgBI CAC'GTC Cuts at: 1318 7952 1318 Size: 6634 6963 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2936 4064 5388 Size: 416 53 83 399 24 1519 1128 1324 Cuts at: 5388 5592 6399 7854 8982 9109 9226 9575 10705 Size: 204 807 1455 1128 127 117 349 1130 Cuts at: 10705 11328 12286 442 Size: 623 958 1753 Fragments arranged by size: 1753 1519 1455 1324 1130 1128 1128 958 807 623 416 399 349 204 127 117 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 13597 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 8990 9466 8990 Size: 476 13121 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2261 2526 4420 5407 5897 6850 8046 8072 Size: 1022 265 1894 987 490 953 1196 26 Cuts at: 8072 10652 10850 12257 1239 Size: 2580 198 1407 2579 Fragments arranged by size: 2580 2579 1894 1407 1196 1022 987 953 490 265 198 26 Bpu10I CC'TnA_GC Cuts at: 5783 8445 5783 Size: 2662 10935 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4910 5243 5545 11722 12587 12885 4910 Size: 333 302 6177 865 298 5622 Fragments arranged by size: 6177 5622 865 333 302 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2418 4197 5582 6052 9689 9785 10294 Size: 617 919 1779 1385 470 3637 96 509 Cuts at: 10294 12260 882 Size: 1966 2219 Fragments arranged by size: 3637 2219 1966 1779 1385 919 617 509 470 96 BsaAI yAC'GTr Cuts at: 493 1204 10287 11388 493 Size: 711 9083 1101 2702 Fragments arranged by size: 9083 2702 1101 711 BsaBI GATnn'nnATC Cuts at: 13591 13591 Size: 13597 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 11404 11786 12421 275 Size: 53 83 186 936 9871 382 635 1451 Fragments arranged by size: 9871 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3592 8709 10383 12030 12428 12702 12849 13260 3592 Size: 5117 1674 1647 398 274 147 411 3929 Fragments arranged by size: 5117 3929 1674 1647 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2671 2701 3985 4015 6169 Size: 30 165 30 1268 30 1284 30 2154 Cuts at: 6169 6199 6848 6878 1178 Size: 30 649 30 7897 Fragments arranged by size: 7897 2154 1284 1268 649 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 5481 5491 5686 6228 10649 1186 Size: 4295 10 195 542 4421 4134 Fragments arranged by size: 4421 4295 4134 542 195 10 BseYI C'CCAG_C Cuts at: 2183 5246 5835 6993 7017 7876 8489 8744 10625 Size: 3063 589 1158 24 859 613 255 1881 Cuts at: 10625 12800 13296 2183 Size: 2175 496 2484 Fragments arranged by size: 3063 2484 2175 1881 1158 859 613 589 496 255 24 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3669 4080 6894 7215 7646 10387 1163 Size: 573 1933 411 2814 321 431 2741 4373 Fragments arranged by size: 4373 2814 2741 1933 573 431 411 321 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 11047 11808 12836 1540 Size: 275 186 9046 761 1028 2301 Fragments arranged by size: 9046 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 6447 7037 9855 11658 11743 12814 6447 Size: 590 2818 1803 85 1071 7230 Fragments arranged by size: 7230 2818 1803 1071 590 85 BsmI GAATG_Cn' Cuts at: 2477 3504 3987 5677 7601 9433 9556 11108 11201 Size: 1027 483 1690 1924 1832 123 1552 93 Cuts at: 11201 2477 Size: 4873 Fragments arranged by size: 4873 1924 1832 1690 1552 1027 483 123 93 BsmBI CGTCTCn'nnnn_ Cuts at: 7635 7635 Size: 13597 Bsp1286I G_dGCh'C Cuts at: 1391 1790 5363 6447 7037 7191 8481 9855 10916 Size: 399 3573 1084 590 154 1290 1374 1061 Cuts at: 10916 11658 11743 12814 1391 Size: 742 85 1071 2174 Fragments arranged by size: 3573 2174 1374 1290 1084 1071 1061 742 590 399 154 85 BspEI T'CCGG_A Cuts at: 3592 13260 3592 Size: 9668 3929 BspHI T'CATG_A Cuts at: 4318 5773 9225 9783 11486 13216 4318 Size: 1455 3452 558 1703 1730 4699 Fragments arranged by size: 4699 3452 1730 1703 1455 558 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2341 2765 3236 5369 10271 10952 844 Size: 1497 424 471 2133 4902 681 3489 Fragments arranged by size: 4902 3489 2133 1497 681 471 424 BsrBI CCG'CTC Cuts at: 1394 1823 11484 1394 Size: 429 9661 3507 Fragments arranged by size: 9661 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3198 5460 6087 8390 8429 12092 12266 Size: 1183 1950 2262 627 2303 39 3663 174 Cuts at: 12266 65 Size: 1396 Fragments arranged by size: 3663 2303 2262 1950 1396 1183 627 174 39 BsrFI r'CCGG_y Cuts at: 1700 1810 4188 12241 12428 1700 Size: 110 2378 8053 187 2869 Fragments arranged by size: 8053 2869 2378 187 110 BsrGI T'GTAC_A Cuts at: 96 2439 10329 96 Size: 2343 7890 3364 Fragments arranged by size: 7890 3364 2343 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 13597 BssSI C'ACGA_G Cuts at: 11657 12669 11657 Size: 1012 12585 BstAPI GCAn_nnn'nTGC Cuts at: 794 4067 7915 794 Size: 3273 3848 6476 Fragments arranged by size: 6476 3848 3273 BstBI TT'CG_AA Cuts at: 5842 5965 6028 9265 10891 12401 5842 Size: 123 63 3237 1626 1510 7038 Fragments arranged by size: 7038 3237 1626 1510 123 63 BstEII G'GTnAC_C Cuts at: 1184 4698 10145 1184 Size: 3514 5447 4636 Fragments arranged by size: 5447 4636 3514 BstYI r'GATC_y Cuts at: 1972 2605 2860 3539 4834 5854 5944 9186 9330 Size: 633 255 679 1295 1020 90 3242 144 Cuts at: 9330 9447 9463 9486 10377 10767 10927 11294 11679 Size: 117 16 23 891 390 160 367 385 Cuts at: 11679 11696 13148 13234 13246 13592 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 3242 1977 1452 1295 1020 891 679 633 390 385 367 346 255 160 144 117 90 86 23 17 16 12 BstZ17I GTA'TAC Cuts at: 8058 8707 8058 Size: 649 12948 Bsu36I CC'TnA_GG Cuts at: 10728 10728 Size: 13597 BtgI C'CryG_G Cuts at: 513 1065 2004 7191 8895 513 Size: 552 939 5187 1704 5215 Fragments arranged by size: 5215 5187 1704 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 12858 Fragments arranged by size: 12858 683 56 BtsI GCAGTG_nn' Cuts at: 3287 4081 4807 6808 6950 7658 8171 9697 9875 Size: 794 726 2001 142 708 513 1526 178 Cuts at: 9875 10656 11113 11907 11927 3287 Size: 781 457 794 20 4957 Fragments arranged by size: 4957 2001 1526 794 794 781 726 708 513 457 178 142 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 13597 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 9867 9902 529 Size: 35 9303 35 4224 Fragments arranged by size: 9303 4224 35 35 DraI TTT'AAA Cuts at: 2259 2616 3601 4050 4231 4545 4980 10901 2259 Size: 357 985 449 181 314 435 5921 4955 Fragments arranged by size: 5921 4955 985 449 435 357 314 181 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 12604 809 Size: 507 11288 1802 Fragments arranged by size: 11288 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3153 6871 7022 Size: 54 1425 50 275 18 1323 3718 151 Cuts at: 7022 7455 8898 10030 11044 11933 13562 8 Size: 433 1443 1132 1014 889 1629 43 Fragments arranged by size: 3718 1629 1443 1425 1323 1132 1014 889 433 275 151 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 11044 1537 Size: 275 9232 4090 Fragments arranged by size: 9232 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2136 2595 3952 5149 6526 7742 7806 8927 Size: 783 459 1357 1197 1377 1216 64 1121 Cuts at: 8927 10578 11527 1353 Size: 1651 949 3423 Fragments arranged by size: 3423 1651 1377 1357 1216 1197 1121 949 783 459 64 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 12184 12558 12704 387 Size: 1064 138 10595 374 146 1280 Fragments arranged by size: 10595 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2261 2502 2526 3990 4420 4814 5407 Size: 393 629 241 24 1464 430 394 593 Cuts at: 5407 5897 6672 6850 7562 8046 8072 8975 10652 Size: 490 775 178 712 484 26 903 1677 Cuts at: 10652 10850 11660 12257 13044 13312 1239 Size: 198 810 597 787 268 1524 Fragments arranged by size: 1677 1524 1464 903 810 787 775 712 629 597 593 490 484 430 394 393 268 241 198 178 26 24 EcoNI CCTnn'n_nnAGG Cuts at: 3243 8516 9767 3243 Size: 5273 1251 7073 Fragments arranged by size: 7073 5273 1251 EcoO109I rG'GnC_Cy Cuts at: 2146 10260 2146 Size: 8114 5483 EcoRI G'AATT_C Cuts at: 10906 11284 10906 Size: 378 13219 EcoRV GAT'ATC Cuts at: 8155 8155 Size: 13597 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2165 3990 7341 8833 10881 2165 Size: 1825 3351 1492 2048 4881 Fragments arranged by size: 4881 3351 2048 1825 1492 FspI TGC'GCA Cuts at: 12103 12103 Size: 13597 HaeII r_GCGC'y Cuts at: 1678 6553 8096 10528 12744 1678 Size: 4875 1543 2432 2216 2531 Fragments arranged by size: 4875 2531 2432 2216 1543 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3506 3538 4448 4480 4807 Size: 32 135 32 1934 32 910 32 327 Cuts at: 4807 4839 5143 5175 5431 5463 5902 5934 6503 Size: 32 304 32 256 32 439 32 569 Cuts at: 6503 6535 6998 7030 7702 7734 7846 7878 8823 Size: 32 463 32 672 32 112 32 945 Cuts at: 8823 8855 8956 8988 9439 9471 9997 10029 10823 Size: 32 101 32 451 32 526 32 794 Cuts at: 10823 10855 12238 12270 12312 12344 1373 Size: 32 1383 32 42 32 2626 Fragments arranged by size: 2626 1934 1383 945 910 794 672 569 526 463 451 439 327 304 256 135 112 101 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2330 4015 7362 7559 7624 10716 Size: 988 281 392 1685 3347 197 65 3092 Cuts at: 10716 10941 11187 669 Size: 225 246 3079 Fragments arranged by size: 3347 3092 3079 1685 988 392 281 246 225 197 65 HindIII A'AGCT_T Cuts at: 748 6646 6757 7831 9516 10106 748 Size: 5898 111 1074 1685 590 4239 Fragments arranged by size: 5898 4239 1685 1074 590 111 HpaI GTT'AAC Cuts at: 4015 11187 4015 Size: 7172 6425 KpnI G_GTAC'C Cuts at: 10922 10922 Size: 13597 MfeI C'AATT_G Cuts at: 11196 11196 Size: 13597 MluI A'CGCG_T Cuts at: 13473 13473 Size: 13597 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2824 3079 3781 6413 6556 8086 9158 10254 Size: 1519 255 702 2632 143 1530 1072 1096 Cuts at: 10254 12711 12895 1305 Size: 2457 184 2007 Fragments arranged by size: 2632 2457 2007 1530 1519 1096 1072 702 255 184 143 MscI TGG'CCA Cuts at: 10 64 1489 1832 3155 7457 8900 10032 10 Size: 54 1425 343 1323 4302 1443 1132 3575 Fragments arranged by size: 4302 3575 1443 1425 1323 1132 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 5526 5583 8076 8661 9141 10814 11555 Size: 648 4360 57 2493 585 480 1673 741 Cuts at: 11555 11914 12073 518 Size: 359 159 2042 Fragments arranged by size: 4360 2493 2042 1673 741 648 585 480 359 159 57 MspA1I CmG'CkG Cuts at: 1606 2671 2945 5246 6182 6843 7021 7162 7371 Size: 1065 274 2301 936 661 178 141 209 Cuts at: 7371 8744 9284 11013 11690 12838 13083 1606 Size: 1373 540 1729 677 1148 245 2120 Fragments arranged by size: 2301 2120 1729 1373 1148 1065 936 677 661 540 274 245 209 178 141 NcoI C'CATG_G Cuts at: 513 1065 2004 7191 8895 513 Size: 552 939 5187 1704 5215 Fragments arranged by size: 5215 5187 1704 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 13597 NheI G'CTAG_C Cuts at: 1052 1052 Size: 13597 NotI GC'GGCC_GC Cuts at: 11044 11044 Size: 13597 NsiI A_TGCA'T Cuts at: 3506 6288 3506 Size: 2782 10815 NspI r_CATG'y Cuts at: 1132 1229 3504 4188 5693 6290 6619 8740 9535 Size: 97 2275 684 1505 597 329 2121 795 Cuts at: 9535 10955 12500 1132 Size: 1420 1545 2229 Fragments arranged by size: 2275 2229 2121 1545 1505 1420 795 684 597 329 97 PasI CC'CwG_GG Cuts at: 1348 7279 1348 Size: 5931 7666 PciI A'CATG_T Cuts at: 6615 12496 6615 Size: 5881 7716 PflMI CCAn_nnn'nTGG Cuts at: 1108 6149 8896 1108 Size: 5041 2747 5809 Fragments arranged by size: 5809 5041 2747 PfoI T'CCnGG_A Cuts at: 6534 8062 10511 6534 Size: 1528 2449 9620 Fragments arranged by size: 9620 2449 1528 PmeI GTTT'AAAC Cuts at: 10901 10901 Size: 13597 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 11666 11698 13179 13211 11666 Size: 32 1481 32 12052 Fragments arranged by size: 12052 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2146 10260 2146 Size: 8114 5483 PshAI GACnn'nnGTC Cuts at: 1613 9701 1613 Size: 8088 5509 PsiI TTA'TAA Cuts at: 4476 8769 9366 11167 4476 Size: 4293 597 1801 6906 Fragments arranged by size: 6906 4293 1801 597 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2719 2751 2923 2955 2974 3006 7083 7115 2719 Size: 32 172 32 19 32 4077 32 9201 Fragments arranged by size: 9201 4077 172 32 32 32 32 19 PstI C_TGCA'G Cuts at: 830 6848 7161 7312 9249 9289 10949 830 Size: 6018 313 151 1937 40 1660 3478 Fragments arranged by size: 6018 3478 1937 1660 313 151 40 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 13597 PvuII CAG'CTG Cuts at: 2671 5246 6182 6843 7371 8744 9284 2671 Size: 2575 936 661 528 1373 540 6984 Fragments arranged by size: 6984 2575 1373 936 661 540 528 RsrII CG'GwC_CG Cuts at: 10039 10039 Size: 13597 SalI G'TCGA_C Cuts at: 1936 2328 7557 10939 1936 Size: 392 5229 3382 4594 Fragments arranged by size: 5229 4594 3382 392 SapI GCTCTTCn'nnn_ Cuts at: 3952 6526 7806 3952 Size: 2574 1280 9743 Fragments arranged by size: 9743 2574 1280 SbfI CC_TGCA'GG Cuts at: 10949 10949 Size: 13597 ScaI AGT'ACT Cuts at: 2086 4108 6130 7078 9563 11845 13557 2086 Size: 2022 2022 948 2485 2282 1712 2126 Fragments arranged by size: 2485 2282 2126 2022 2022 1712 948 SfcI C'TryA_G Cuts at: 826 1045 2736 2787 2940 2991 3768 4873 5019 Size: 219 1691 51 153 51 777 1105 146 Cuts at: 5019 5718 6553 6844 7157 7308 8007 9245 9285 Size: 699 835 291 313 151 699 1238 40 Cuts at: 9285 10251 10945 12080 12761 12952 826 Size: 966 694 1135 681 191 1471 Fragments arranged by size: 1691 1471 1238 1135 1105 966 835 777 699 699 694 681 313 291 219 191 153 151 146 51 51 40 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 13597 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 13597 SmaI CCC'GGG Cuts at: 1872 10924 1872 Size: 9052 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3311 4889 5222 5524 11701 12602 Size: 197 926 1368 1578 333 302 6177 901 Cuts at: 12602 12864 820 Size: 262 1553 Fragments arranged by size: 6177 1578 1553 1368 926 901 333 302 262 197 SnaBI TAC'GTA Cuts at: 493 10287 11388 493 Size: 9794 1101 2702 Fragments arranged by size: 9794 2702 1101 SpeI A'CTAG_T Cuts at: 152 2645 4022 4109 5345 152 Size: 2493 1377 87 1236 8404 Fragments arranged by size: 8404 2493 1377 1236 87 SphI G_CATG'C Cuts at: 1132 6290 10955 1132 Size: 5158 4665 3774 Fragments arranged by size: 5158 4665 3774 SspI AAT'ATT Cuts at: 5 52 9151 9298 11521 5 Size: 47 9099 147 2223 2081 Fragments arranged by size: 9099 2223 2081 147 47 StuI AGG'CCT Cuts at: 1517 4495 4681 5465 6356 9614 9883 1517 Size: 2978 186 784 891 3258 269 5231 Fragments arranged by size: 5231 3258 2978 891 784 269 186 StyI C'CwwG_G Cuts at: 513 1065 2004 2360 3156 3952 6708 6766 7191 Size: 552 939 356 796 796 2756 58 425 Cuts at: 7191 7931 8512 8895 9878 10242 11036 513 Size: 740 581 383 983 364 794 3074 Fragments arranged by size: 3074 2756 983 939 796 796 794 740 581 552 425 383 364 356 58 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2373 4046 7287 8407 11624 11641 11794 Size: 1255 793 1673 3241 1120 3217 17 153 Cuts at: 11794 11979 12424 325 Size: 185 445 1498 Fragments arranged by size: 3241 3217 1673 1498 1255 1120 793 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2084 2439 2545 Size: 274 80 33 51 1443 107 355 106 Cuts at: 2545 3326 4106 4723 6128 6973 7076 9561 10329 Size: 781 780 617 1405 845 103 2485 768 Cuts at: 10329 11843 13555 96 Size: 1514 1712 138 Fragments arranged by size: 2485 1712 1514 1443 1405 845 781 780 768 617 355 274 138 107 106 103 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2761 2793 4415 4447 4741 4773 6214 6246 6250 Size: 32 1622 32 294 32 1441 32 4 Cuts at: 6250 6282 8299 8331 10169 10201 10252 10284 13557 Size: 32 2017 32 1838 32 51 32 3273 Cuts at: 13557 13589 2761 Size: 32 2769 Fragments arranged by size: 3273 2769 2017 1838 1622 1441 294 51 32 32 32 32 32 32 32 32 32 4 Tth111I GACn'n_nGTC Cuts at: 1316 7478 1316 Size: 6162 7435 XbaI T'CTAG_A Cuts at: 8428 10933 8428 Size: 2505 11092 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3839 3839 Size: 13597 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 13597 XmaI C'CCGG_G Cuts at: 1870 10922 1870 Size: 9052 4545 XmnI GAAnn'nnTTC Cuts at: 8436 10212 10587 11726 8436 Size: 1776 375 1139 10307 Fragments arranged by size: 10307 1776 1139 375 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 11405 276 Size: 53 83 186 936 9871 2468 Fragments arranged by size: 9871 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII RsrII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: ApaI AscI BaeI BbeI BsiWI BstXI DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI