(Linear) (Six-base) MAPSORT of: KIEE3830.seq Check: 594 from: 1 to: 8892 KIEE3830.orf With 182 enzymes: SgfI * March 22, 2012 16:47 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 760 8892 Size: 760 8132 AccI GT'mk_AC Cuts at: 0 324 5553 6052 6227 6701 7599 7937 8892 Size: 324 5229 499 175 474 898 338 955 Fragments arranged by size: 5229 955 898 499 474 338 324 175 AclI AA'CG_TT Cuts at: 0 1835 2231 8892 Size: 1835 396 6661 Fragments arranged by size: 6661 1835 396 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 497 1985 2809 4667 5557 6970 8892 Size: 497 1488 824 1858 890 1413 1922 Fragments arranged by size: 1922 1858 1488 1413 890 824 497 AfeI AGC'GCT Cuts at: 0 4546 8521 8892 Size: 4546 3975 371 Fragments arranged by size: 4546 3975 371 AflII C'TTAA_G Cuts at: 0 1306 8892 Size: 1306 7586 AflIII A'CryG_T Cuts at: 0 4610 8892 Size: 4610 4282 AhdI GACnn_n'nnGTC Cuts at: 0 2457 8892 Size: 2457 6435 AleI CACnn'nnGTG Cuts at: 0 3521 7136 8892 Size: 3521 3615 1756 Fragments arranged by size: 3615 3521 1756 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 8863 8892 Size: 8863 29 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 4652 4684 8892 Size: 4652 32 4208 Fragments arranged by size: 4652 4208 32 AlwNI CAG_nnn'CTG Cuts at: 0 2062 7702 8892 Size: 2062 5640 1190 Fragments arranged by size: 5640 2062 1190 ApoI r'AATT_y Cuts at: 0 249 1781 3504 3646 3899 6212 7337 8892 Size: 249 1532 1723 142 253 2313 1125 1555 Fragments arranged by size: 2313 1723 1555 1532 1125 253 249 142 AseI AT'TA_AT Cuts at: 0 566 950 1463 2932 8892 Size: 566 384 513 1469 5960 Fragments arranged by size: 5960 1469 566 513 384 AvaI C'yCGr_G Cuts at: 0 3935 7304 8892 Size: 3935 3369 1588 Fragments arranged by size: 3935 3369 1588 AvrII C'CTAG_G Cuts at: 0 6507 8892 Size: 6507 2385 BamHI G'GATC_C Cuts at: 0 600 2829 3849 8892 Size: 600 2229 1020 5043 Fragments arranged by size: 5043 2229 1020 600 BanI G'GyrC_C Cuts at: 0 4451 8214 8892 Size: 4451 3763 678 Fragments arranged by size: 4451 3763 678 BanII G_rGCy'C Cuts at: 0 3358 5186 6476 8892 Size: 3358 1828 1290 2416 Fragments arranged by size: 3358 2416 1828 1290 BbsI GAAGACnn'nnnn_ Cuts at: 0 484 686 2974 3323 5051 7209 8892 Size: 484 202 2288 349 1728 2158 1683 Fragments arranged by size: 2288 2158 1728 1683 484 349 202 BbvCI CC'TCA_GC Cuts at: 0 3778 6440 8892 Size: 3778 2662 2452 Fragments arranged by size: 3778 2662 2452 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 4003 4037 7231 7265 8892 Size: 4003 34 3194 34 1627 Fragments arranged by size: 4003 3194 1627 34 34 BciVI GTATCCnnnnn_n' Cuts at: 0 4220 6389 8892 Size: 4220 2169 2503 Fragments arranged by size: 4220 2503 2169 BclI T'GATC_A Cuts at: 0 758 1247 8418 8892 Size: 758 489 7171 474 Fragments arranged by size: 7171 758 489 474 BfrBI ATG'CAT Cuts at: 0 1499 4281 8892 Size: 1499 2782 4611 Fragments arranged by size: 4611 2782 1499 BglI GCCn_nnn'nGGC Cuts at: 0 2682 6371 8892 Size: 2682 3689 2521 Fragments arranged by size: 3689 2682 2521 BglII A'GATC_T Cuts at: 0 3939 7325 7442 7458 8762 8892 Size: 3939 3386 117 16 1304 130 Fragments arranged by size: 3939 3386 1304 130 117 16 BlpI GC'TnA_GC Cuts at: 0 1000 3721 8892 Size: 1000 2721 5171 Fragments arranged by size: 5171 2721 1000 BmgBI CAC'GTC Cuts at: 0 5947 8892 Size: 5947 2945 BmrI ACTGGGnnnn_n' Cuts at: 0 931 2059 3383 3587 4394 5849 6977 7104 Size: 931 1128 1324 204 807 1455 1128 127 Cuts at: 7104 7221 7570 8700 8892 Size: 117 349 1130 192 Fragments arranged by size: 1455 1324 1130 1128 1128 931 807 349 204 192 127 117 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 6985 7461 8892 Size: 6985 476 1431 Fragments arranged by size: 6985 1431 476 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 256 521 2415 3402 3892 4845 6041 6067 Size: 256 265 1894 987 490 953 1196 26 Cuts at: 6067 8647 8845 8892 Size: 2580 198 47 Fragments arranged by size: 2580 1894 1196 987 953 490 265 256 198 47 26 Bpu10I CC'TnA_GC Cuts at: 0 3778 6440 8892 Size: 3778 2662 2452 Fragments arranged by size: 3778 2662 2452 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2905 3238 3540 8892 Size: 2905 333 302 5352 Fragments arranged by size: 5352 2905 333 302 BsaI GGTCTCn'nnnn_ Cuts at: 0 413 2192 3577 4047 7684 7780 8289 8892 Size: 413 1779 1385 470 3637 96 509 603 Fragments arranged by size: 3637 1779 1385 603 509 470 413 96 BsaAI yAC'GTr Cuts at: 0 8282 8892 Size: 8282 610 BsaWI w'CCGG_w Cuts at: 0 1587 6704 8378 8892 Size: 1587 5117 1674 514 Fragments arranged by size: 5117 1674 1587 514 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 666 696 1980 2010 4164 4194 4843 4873 Size: 666 30 1284 30 2154 30 649 30 Cuts at: 4873 8892 Size: 4019 Fragments arranged by size: 4019 2154 1284 666 649 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 3476 3486 3681 4223 8644 8892 Size: 3476 10 195 542 4421 248 Fragments arranged by size: 4421 3476 542 248 195 10 BseYI C'CCAG_C Cuts at: 0 178 3241 3830 4988 5012 5871 6484 6739 Size: 178 3063 589 1158 24 859 613 255 Cuts at: 6739 8620 8892 Size: 1881 272 Fragments arranged by size: 3063 1881 1158 859 613 589 272 255 178 24 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1664 2075 4889 5210 5641 8382 8892 Size: 1664 411 2814 321 431 2741 510 Fragments arranged by size: 2814 2741 1664 510 431 411 321 BsiHKAI G_wGCw'C Cuts at: 0 4442 5032 7850 8892 Size: 4442 590 2818 1042 Fragments arranged by size: 4442 2818 1042 590 BsmI GAATG_Cn' Cuts at: 0 472 1499 1982 3672 5596 7428 7551 8892 Size: 472 1027 483 1690 1924 1832 123 1341 Fragments arranged by size: 1924 1832 1690 1341 1027 483 472 123 BsmBI CGTCTCn'nnnn_ Cuts at: 0 5630 8892 Size: 5630 3262 Bsp1286I G_dGCh'C Cuts at: 0 3358 4442 5032 5186 6476 7850 8892 Size: 3358 1084 590 154 1290 1374 1042 Fragments arranged by size: 3358 1374 1290 1084 1042 590 154 BspEI T'CCGG_A Cuts at: 0 1587 8892 Size: 1587 7305 BspHI T'CATG_A Cuts at: 0 2313 3768 7220 7778 8892 Size: 2313 1455 3452 558 1114 Fragments arranged by size: 3452 2313 1455 1114 558 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 336 760 1231 3364 8266 8892 Size: 336 424 471 2133 4902 626 Fragments arranged by size: 4902 2133 626 471 424 336 BsrDI GCAATG_nn' Cuts at: 0 1193 3455 4082 6385 6424 8892 Size: 1193 2262 627 2303 39 2468 Fragments arranged by size: 2468 2303 2262 1193 627 39 BsrFI r'CCGG_y Cuts at: 0 2183 8892 Size: 2183 6709 BsrGI T'GTAC_A Cuts at: 0 434 8324 8892 Size: 434 7890 568 Fragments arranged by size: 7890 568 434 BstAPI GCAn_nnn'nTGC Cuts at: 0 2062 5910 8892 Size: 2062 3848 2982 Fragments arranged by size: 3848 2982 2062 BstBI TT'CG_AA Cuts at: 0 3837 3960 4023 7260 8886 8892 Size: 3837 123 63 3237 1626 6 Fragments arranged by size: 3837 3237 1626 123 63 6 BstEII G'GTnAC_C Cuts at: 0 2693 8140 8892 Size: 2693 5447 752 Fragments arranged by size: 5447 2693 752 BstYI r'GATC_y Cuts at: 0 600 855 1534 2829 3849 3939 7181 7325 Size: 600 255 679 1295 1020 90 3242 144 Cuts at: 7325 7442 7458 7481 8372 8762 8892 Size: 117 16 23 891 390 130 Fragments arranged by size: 3242 1295 1020 891 679 600 390 255 144 130 117 90 23 16 BstZ17I GTA'TAC Cuts at: 0 6053 6702 8892 Size: 6053 649 2190 Fragments arranged by size: 6053 2190 649 Bsu36I CC'TnA_GG Cuts at: 0 8723 8892 Size: 8723 169 BtgI C'CryG_G Cuts at: 0 5186 6890 8892 Size: 5186 1704 2002 Fragments arranged by size: 5186 2002 1704 BtsI GCAGTG_nn' Cuts at: 0 1282 2076 2802 4803 4945 5653 6166 7692 Size: 1282 794 726 2001 142 708 513 1526 Cuts at: 7692 7870 8651 8892 Size: 178 781 241 Fragments arranged by size: 2001 1526 1282 794 781 726 708 513 241 178 142 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 7862 7897 8892 Size: 7862 35 995 Fragments arranged by size: 7862 995 35 DraI TTT'AAA Cuts at: 0 254 611 1596 2045 2226 2540 2975 8892 Size: 254 357 985 449 181 314 435 5917 Fragments arranged by size: 5917 985 449 435 357 314 254 181 EaeI y'GGCC_r Cuts at: 0 1148 4866 5017 5450 6893 8025 8892 Size: 1148 3718 151 433 1443 1132 867 Fragments arranged by size: 3718 1443 1148 1132 867 433 151 EarI CTCTTCn'nnn_ Cuts at: 0 131 590 1947 3144 4521 5737 5801 6922 Size: 131 459 1357 1197 1377 1216 64 1121 Cuts at: 6922 8573 8892 Size: 1651 319 Fragments arranged by size: 1651 1377 1357 1216 1197 1121 459 319 131 64 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 256 497 521 1985 2415 2809 3402 3892 Size: 256 241 24 1464 430 394 593 490 Cuts at: 3892 4667 4845 5557 6041 6067 6970 8647 8845 Size: 775 178 712 484 26 903 1677 198 Cuts at: 8845 8892 Size: 47 Fragments arranged by size: 1677 1464 903 775 712 593 490 484 430 394 256 241 198 178 47 26 24 EcoNI CCTnn'n_nnAGG Cuts at: 0 1238 6511 7762 8892 Size: 1238 5273 1251 1130 Fragments arranged by size: 5273 1251 1238 1130 EcoO109I rG'GnC_Cy Cuts at: 0 141 8255 8892 Size: 141 8114 637 Fragments arranged by size: 8114 637 141 EcoRV GAT'ATC Cuts at: 0 6150 8892 Size: 6150 2742 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 160 1985 5336 6828 8876 8892 Size: 160 1825 3351 1492 2048 16 Fragments arranged by size: 3351 2048 1825 1492 160 16 HaeII r_GCGC'y Cuts at: 0 4548 6091 8523 8892 Size: 4548 1543 2432 369 Fragments arranged by size: 4548 2432 1543 369 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1501 1533 2443 2475 2802 2834 3138 3170 Size: 1501 32 910 32 327 32 304 32 Cuts at: 3170 3426 3458 3897 3929 4498 4530 4993 5025 Size: 256 32 439 32 569 32 463 32 Cuts at: 5025 5697 5729 5841 5873 6818 6850 6951 6983 Size: 672 32 112 32 945 32 101 32 Cuts at: 6983 7434 7466 7992 8024 8818 8850 8892 Size: 451 32 526 32 794 32 42 Fragments arranged by size: 1501 945 910 794 672 569 526 463 451 439 327 304 256 112 101 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 325 2010 5357 5554 5619 8711 8892 Size: 325 1685 3347 197 65 3092 181 Fragments arranged by size: 3347 3092 1685 325 197 181 65 HindIII A'AGCT_T Cuts at: 0 4641 4752 5826 7511 8101 8892 Size: 4641 111 1074 1685 590 791 Fragments arranged by size: 4641 1685 1074 791 590 111 HpaI GTT'AAC Cuts at: 0 2010 8892 Size: 2010 6882 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 819 1074 1776 4408 4551 6081 7153 8249 Size: 819 255 702 2632 143 1530 1072 1096 Cuts at: 8249 8892 Size: 643 Fragments arranged by size: 2632 1530 1096 1072 819 702 643 255 143 MscI TGG'CCA Cuts at: 0 1150 5452 6895 8027 8892 Size: 1150 4302 1443 1132 865 Fragments arranged by size: 4302 1443 1150 1132 865 MslI CAynn'nnrTG Cuts at: 0 3521 3578 6071 6656 7136 8809 8892 Size: 3521 57 2493 585 480 1673 83 Fragments arranged by size: 3521 2493 1673 585 480 83 57 MspA1I CmG'CkG Cuts at: 0 666 940 3241 4177 4838 5016 5157 5366 Size: 666 274 2301 936 661 178 141 209 Cuts at: 5366 6739 7279 8892 Size: 1373 540 1613 Fragments arranged by size: 2301 1613 1373 936 666 661 540 274 209 178 141 NcoI C'CATG_G Cuts at: 0 5186 6890 8892 Size: 5186 1704 2002 Fragments arranged by size: 5186 2002 1704 NsiI A_TGCA'T Cuts at: 0 1501 4283 8892 Size: 1501 2782 4609 Fragments arranged by size: 4609 2782 1501 NspI r_CATG'y Cuts at: 0 1499 2183 3688 4285 4614 6735 7530 8892 Size: 1499 684 1505 597 329 2121 795 1362 Fragments arranged by size: 2121 1505 1499 1362 795 684 597 329 PasI CC'CwG_GG Cuts at: 0 5274 8892 Size: 5274 3618 PciI A'CATG_T Cuts at: 0 4610 8892 Size: 4610 4282 PflMI CCAn_nnn'nTGG Cuts at: 0 4144 6891 8892 Size: 4144 2747 2001 Fragments arranged by size: 4144 2747 2001 PfoI T'CCnGG_A Cuts at: 0 4529 6057 8506 8892 Size: 4529 1528 2449 386 Fragments arranged by size: 4529 2449 1528 386 PpuMI rG'GwC_Cy Cuts at: 0 141 8255 8892 Size: 141 8114 637 Fragments arranged by size: 8114 637 141 PshAI GACnn'nnGTC Cuts at: 0 7696 8892 Size: 7696 1196 PsiI TTA'TAA Cuts at: 0 2471 6764 7361 8892 Size: 2471 4293 597 1531 Fragments arranged by size: 4293 2471 1531 597 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 714 746 918 950 969 1001 5078 5110 Size: 714 32 172 32 19 32 4077 32 Cuts at: 5110 8892 Size: 3782 Fragments arranged by size: 4077 3782 714 172 32 32 32 32 19 PstI C_TGCA'G Cuts at: 0 4843 5156 5307 7244 7284 8892 Size: 4843 313 151 1937 40 1608 Fragments arranged by size: 4843 1937 1608 313 151 40 PvuII CAG'CTG Cuts at: 0 666 3241 4177 4838 5366 6739 7279 8892 Size: 666 2575 936 661 528 1373 540 1613 Fragments arranged by size: 2575 1613 1373 936 666 661 540 528 RsrII CG'GwC_CG Cuts at: 0 8034 8892 Size: 8034 858 SalI G'TCGA_C Cuts at: 0 323 5552 8892 Size: 323 5229 3340 Fragments arranged by size: 5229 3340 323 SapI GCTCTTCn'nnn_ Cuts at: 0 1947 4521 5801 8892 Size: 1947 2574 1280 3091 Fragments arranged by size: 3091 2574 1947 1280 ScaI AGT'ACT Cuts at: 0 81 2103 4125 5073 7558 8892 Size: 81 2022 2022 948 2485 1334 Fragments arranged by size: 2485 2022 2022 1334 948 81 SfcI C'TryA_G Cuts at: 0 731 782 935 986 1763 2868 3014 3713 Size: 731 51 153 51 777 1105 146 699 Cuts at: 3713 4548 4839 5152 5303 6002 7240 7280 8246 Size: 835 291 313 151 699 1238 40 966 Cuts at: 8246 8892 Size: 646 Fragments arranged by size: 1238 1105 966 835 777 731 699 699 646 313 291 153 151 146 51 51 40 SmlI C'TyrA_G Cuts at: 0 1306 2884 3217 3519 8892 Size: 1306 1578 333 302 5373 Fragments arranged by size: 5373 1578 1306 333 302 SnaBI TAC'GTA Cuts at: 0 8282 8892 Size: 8282 610 SpeI A'CTAG_T Cuts at: 0 640 2017 2104 3340 8892 Size: 640 1377 87 1236 5552 Fragments arranged by size: 5552 1377 1236 640 87 SphI G_CATG'C Cuts at: 0 4285 8892 Size: 4285 4607 SspI AAT'ATT Cuts at: 0 7146 7293 8892 Size: 7146 147 1599 Fragments arranged by size: 7146 1599 147 StuI AGG'CCT Cuts at: 0 2490 2676 3460 4351 7609 7878 8892 Size: 2490 186 784 891 3258 269 1014 Fragments arranged by size: 3258 2490 1014 891 784 269 186 StyI C'CwwG_G Cuts at: 0 355 1151 1947 4703 4761 5186 5926 6507 Size: 355 796 796 2756 58 425 740 581 Cuts at: 6507 6890 7873 8237 8892 Size: 383 983 364 655 Fragments arranged by size: 2756 983 796 796 740 655 581 425 383 364 355 58 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 368 2041 5282 6402 8892 Size: 368 1673 3241 1120 2490 Fragments arranged by size: 3241 2490 1673 1120 368 TatI w'GTAC_w Cuts at: 0 79 434 540 1321 2101 2718 4123 4968 Size: 79 355 106 781 780 617 1405 845 Cuts at: 4968 5071 7556 8324 8892 Size: 103 2485 768 568 Fragments arranged by size: 2485 1405 845 781 780 768 617 568 355 106 103 79 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 756 788 2410 2442 2736 2768 4209 4241 Size: 756 32 1622 32 294 32 1441 32 Cuts at: 4241 4245 4277 6294 6326 8164 8196 8247 8279 Size: 4 32 2017 32 1838 32 51 32 Cuts at: 8279 8892 Size: 613 Fragments arranged by size: 2017 1838 1622 1441 756 613 294 51 32 32 32 32 32 32 32 32 4 Tth111I GACn'n_nGTC Cuts at: 0 5473 8892 Size: 5473 3419 XbaI T'CTAG_A Cuts at: 0 6423 8892 Size: 6423 2469 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1834 8892 Size: 1834 7058 XmnI GAAnn'nnTTC Cuts at: 0 6431 8207 8582 8892 Size: 6431 1776 375 310 Fragments arranged by size: 6431 1776 375 310 Enzymes that do cut: AarI AccI AclI AcuI AfeI AflII AflIII AhdI AleI AlfI AloI AlwNI ApoI AseI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI BsrGI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtsI CspCI DraI EaeI EarI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I HincII HindIII HpaI MmeI MscI MslI MspA1I NcoI NsiI NspI PasI PciI PflMI PfoI PpuMI PshAI PsiI PsrI PstI PvuII RsrII SalI SapI ScaI SfcI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmnI Enzymes that do not cut: AatII Acc65I AgeI ApaI ApaLI AscI AsiSI BaeI BbeI Bme1580I BmtI BsaBI BsaHI BsiEI BsiWI BsrBI BssHII BssSI BstXI BtgZI ClaI DraIII DrdI EagI EciI EcoICRI EcoRI FseI FspI FspAI KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PmeI PmlI PpiI PspOMI PspXI PvuI SacI SacII SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SrfI SwaI XhoI XmaI ZraI