Restriction map for
pFN21AE2530
(Linear) (Six-base) MAPSORT of: KIEE2530.seq Check: 4155 from: 1 to: 9366
KIEE2530.orf
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 384 9366
Size: 384 8982
AatII G_ACGT'C
Cuts at: 0 2306 6790 9366
Size: 2306 4484 2576
Fragments arranged by size:
4484 2576 2306
AccI GT'mk_AC
Cuts at: 0 2188 9366
Size: 2188 7178
Acc65I G'GTAC_C
Cuts at: 0 6686 7070 9366
Size: 6686 384 2296
Fragments arranged by size:
6686 2296 384
AclI AA'CG_TT
Cuts at: 0 2825 9366
Size: 2825 6541
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1070 1176 1958 2076 2423 3548 3583 5134
Size: 1070 106 782 118 347 1125 35 1551
Cuts at: 5134 5240 5896 5907 6214 6431 7486 8457 9292
Size: 106 656 11 307 217 1055 971 835
Cuts at: 9292 9366
Size: 74
Fragments arranged by size:
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347 307 217 118 106 106 74 35
11
AflIII A'CryG_T
Cuts at: 0 169 213 937 1174 1854 6645 6882 8797
Size: 169 44 724 237 680 4791 237 1915
Cuts at: 8797 9366
Size: 569
Fragments arranged by size:
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44
AgeI A'CCGG_T
Cuts at: 0 8839 9366
Size: 8839 527
AhdI GACnn_n'nnGTC
Cuts at: 0 2643 8243 9366
Size: 2643 5600 1123
Fragments arranged by size:
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AleI CACnn'nnGTG
Cuts at: 0 2339 9366
Size: 2339 7027
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 521 3890 4706 9366
Size: 521 3369 816 4660
Fragments arranged by size:
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AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1844 1876 8598 8630 9366
Size: 1844 32 6722 32 736
Fragments arranged by size:
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AlwNI CAG_nnn'CTG
Cuts at: 0 2158 3494 5071 9366
Size: 2158 1336 1577 4295
Fragments arranged by size:
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ApaI G_GGCC'C
Cuts at: 0 4884 9366
Size: 4884 4482
ApaLI G'TGCA_C
Cuts at: 0 3131 3399 4731 9366
Size: 3131 268 1332 4635
Fragments arranged by size:
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ApoI r'AATT_y
Cuts at: 0 700 2017 2037 3250 3454 4038 5038 5136
Size: 700 1317 20 1213 204 584 1000 98
Cuts at: 5136 6300 6568 6948 7286 7499 8002 8576 8893
Size: 1164 268 380 338 213 503 574 317
Cuts at: 8893 8899 9366
Size: 6 467
Fragments arranged by size:
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467 380 338 317 268 213 204 98
20 6
AseI AT'TA_AT
Cuts at: 0 1136 9366
Size: 1136 8230
AvaI C'yCGr_G
Cuts at: 0 49 3782 3953 3974 5057 5114 5282 5876
Size: 49 3733 171 21 1083 57 168 594
Cuts at: 5876 9366
Size: 3490
Fragments arranged by size:
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21
AvrII C'CTAG_G
Cuts at: 0 2218 7720 9366
Size: 2218 5502 1646
Fragments arranged by size:
5502 2218 1646
BamHI G'GATC_C
Cuts at: 0 740 1318 1437 3196 9366
Size: 740 578 119 1759 6170
Fragments arranged by size:
6170 1759 740 578 119
BanI G'GyrC_C
Cuts at: 0 1311 2957 4243 6686 7070 9366
Size: 1311 1646 1286 2443 384 2296
Fragments arranged by size:
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BanII G_rGCy'C
Cuts at: 0 2104 2699 4884 6173 6741 7178 7216 7616
Size: 2104 595 2185 1289 568 437 38 400
Cuts at: 7616 9116 9366
Size: 1500 250
Fragments arranged by size:
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250 38
BbsI GAAGACnn'nnnn_
Cuts at: 0 2356 2411 3986 5576 5902 5981 6437 7349
Size: 2356 55 1575 1590 326 79 456 912
Cuts at: 7349 9366
Size: 2017
Fragments arranged by size:
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55
BbvCI CC'TCA_GC
Cuts at: 0 1691 4874 9366
Size: 1691 3183 4492
Fragments arranged by size:
4492 3183 1691
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3074 3108 6268 6302 8277 8311 9366
Size: 3074 34 3160 34 1975 34 1055
Fragments arranged by size:
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BciVI GTATCCnnnnn_n'
Cuts at: 0 4354 9366
Size: 4354 5012
BclI T'GATC_A
Cuts at: 0 954 3384 3821 7829 9366
Size: 954 2430 437 4008 1537
Fragments arranged by size:
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BfrBI ATG'CAT
Cuts at: 0 3089 7045 9366
Size: 3089 3956 2321
Fragments arranged by size:
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BglI GCCn_nnn'nGGC
Cuts at: 0 53 3449 9366
Size: 53 3396 5917
Fragments arranged by size:
5917 3396 53
BglII A'GATC_T
Cuts at: 0 3678 5838 9366
Size: 3678 2160 3528
Fragments arranged by size:
3678 3528 2160
BlpI GC'TnA_GC
Cuts at: 0 3569 9366
Size: 3569 5797
Bme1580I G_kGCm'C
Cuts at: 0 2179 3000 3135 3403 4246 4530 4613 4735
Size: 2179 821 135 268 843 284 83 122
Cuts at: 4735 4884 6770 7190 7809 8133 9366
Size: 149 1886 420 619 324 1233
Fragments arranged by size:
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284 268 149 135 122 83
BmgBI CAC'GTC
Cuts at: 0 3551 4096 6646 9366
Size: 3551 545 2550 2720
Fragments arranged by size:
3551 2720 2550 545
BmrI ACTGGGnnnn_n'
Cuts at: 0 332 566 3089 3401 3513 6719 6863 8224
Size: 332 234 2523 312 112 3206 144 1361
Cuts at: 8224 9366
Size: 1142
Fragments arranged by size:
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112
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3697 9366
Size: 3697 5669
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 671 1008 1770 1846 2330 2594 2987 4100
Size: 671 337 762 76 484 264 393 1113
Cuts at: 4100 4580 4670 4786 6656 6779 7588 8153 8763
Size: 480 90 116 1870 123 809 565 610
Cuts at: 8763 8942 9322 9366
Size: 179 380 44
Fragments arranged by size:
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480 393 380 337 264 179 123 116
90 76 44
Bpu10I CC'TnA_GC
Cuts at: 0 1691 4874 7215 7595 8192 9366
Size: 1691 3183 2341 380 597 1174
Fragments arranged by size:
3183 2341 1691 1174 597 380
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1914 3742 3800 6057 6376 7455 9202 9366
Size: 1914 1828 58 2257 319 1079 1747 164
Fragments arranged by size:
2257 1914 1828 1747 1079 319 164 58
BsaI GGTCTCn'nnnn_
Cuts at: 0 657 7142 9366
Size: 657 6485 2224
Fragments arranged by size:
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BsaAI yAC'GTr
Cuts at: 0 1105 4640 6883 9366
Size: 1105 3535 2243 2483
Fragments arranged by size:
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BsaBI GATnn'nnATC
Cuts at: 0 643 859 1702 7328 9366
Size: 643 216 843 5626 2038
Fragments arranged by size:
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BsaHI Gr'CG_yC
Cuts at: 0 2303 6787 9366
Size: 2303 4484 2579
Fragments arranged by size:
4484 2579 2303
BsaWI w'CCGG_w
Cuts at: 0 40 2474 3545 4990 8839 9366
Size: 40 2434 1071 1445 3849 527
Fragments arranged by size:
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BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 654 684 1112 1142 1844 1874 2711 2741
Size: 654 30 428 30 702 30 837 30
Cuts at: 2741 4095 4125 6777 6807 9366
Size: 1354 30 2652 30 2559
Fragments arranged by size:
2652 2559 1354 837 702 654 428 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 13 1496 2187 3869 4103 5199 5617 6535
Size: 13 1483 691 1682 234 1096 418 918
Cuts at: 6535 6782 7351 7756 9366
Size: 247 569 405 1610
Fragments arranged by size:
1682 1610 1483 1096 918 691 569 418
405 247 234 13
BseYI C'CCAG_C
Cuts at: 0 1667 1785 2857 4703 6769 8208 9040 9156
Size: 1667 118 1072 1846 2066 1439 832 116
Cuts at: 9156 9357 9366
Size: 201 9
Fragments arranged by size:
2066 1846 1667 1439 1072 832 201 118
116 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 579 1912 2561 2835 3015 3297 4464 4620
Size: 579 1333 649 274 180 282 1167 156
Cuts at: 4620 9366
Size: 4746
Fragments arranged by size:
4746 1333 1167 649 579 282 274 180
156
BsiEI CG_ry'CG
Cuts at: 0 73 9366
Size: 73 9293
BsiHKAI G_wGCw'C
Cuts at: 0 2104 2699 3135 3403 3526 4356 4735 6173
Size: 2104 595 436 268 123 830 379 1438
Cuts at: 6173 7125 7178 7616 7995 9116 9366
Size: 952 53 438 379 1121 250
Fragments arranged by size:
2104 1438 1121 952 830 595 438 436
379 379 268 250 123 53
BsmI GAATG_Cn'
Cuts at: 0 759 1060 1652 2320 6243 6461 8654 9366
Size: 759 301 592 668 3923 218 2193 712
Fragments arranged by size:
3923 2193 759 712 668 592 301 218
BsmBI CGTCTCn'nnnn_
Cuts at: 0 5111 6794 8362 9366
Size: 5111 1683 1568 1004
Fragments arranged by size:
5111 1683 1568 1004
Bsp1286I G_dGCh'C
Cuts at: 0 2104 2179 2699 3000 3135 3403 3526 4246
Size: 2104 75 520 301 135 268 123 720
Cuts at: 4246 4356 4530 4613 4735 4884 6173 6741 6770
Size: 110 174 83 122 149 1289 568 29
Cuts at: 6770 7125 7178 7190 7216 7616 7809 7995 8133
Size: 355 53 12 26 400 193 186 138
Cuts at: 8133 9116 9366
Size: 983 250
Fragments arranged by size:
2104 1289 983 720 568 520 400 355
301 268 250 193 186 174 149 138
135 123 122 110 83 75 53 29
26 12
BspEI T'CCGG_A
Cuts at: 0 40 3545 9366
Size: 40 3505 5821
Fragments arranged by size:
5821 3505 40
BspHI T'CATG_A
Cuts at: 0 4778 7163 7854 9366
Size: 4778 2385 691 1512
Fragments arranged by size:
4778 2385 1512 691
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 384 1934 9366
Size: 384 1550 7432
Fragments arranged by size:
7432 1550 384
BsrBI CCG'CTC
Cuts at: 0 712 9366
Size: 712 8654
BsrDI GCAATG_nn'
Cuts at: 0 1242 1326 2147 2607 3222 4408 5511 6272
Size: 1242 84 821 460 615 1186 1103 761
Cuts at: 6272 6836 8318 9366
Size: 564 1482 1048
Fragments arranged by size:
1482 1242 1186 1103 1048 821 761 615
564 460 84
BsrFI r'CCGG_y
Cuts at: 0 1758 2684 2764 3443 6320 8839 9366
Size: 1758 926 80 679 2877 2519 527
Fragments arranged by size:
2877 2519 1758 926 679 527 80
BsrGI T'GTAC_A
Cuts at: 0 3268 9366
Size: 3268 6098
BssHII G'CGCG_C
Cuts at: 0 1754 9366
Size: 1754 7612
BssSI C'ACGA_G
Cuts at: 0 4927 7145 9366
Size: 4927 2218 2221
Fragments arranged by size:
4927 2221 2218
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1731 2731 3494 6980 9366
Size: 1731 1000 763 3486 2386
Fragments arranged by size:
3486 2386 1731 1000 763
BstBI TT'CG_AA
Cuts at: 0 6465 9366
Size: 6465 2901
BstEII G'GTnAC_C
Cuts at: 0 8666 9366
Size: 8666 700
BstXI CCAn_nnnn'nTGG
Cuts at: 0 5986 7255 9324 9366
Size: 5986 1269 2069 42
Fragments arranged by size:
5986 2069 1269 42
BstYI r'GATC_y
Cuts at: 0 740 1318 1437 3196 3678 5310 5838 6030
Size: 740 578 119 1759 482 1632 528 192
Cuts at: 6030 6252 6631 9265 9366
Size: 222 379 2634 101
Fragments arranged by size:
2634 1759 1632 740 578 528 482 379
222 192 119 101
BstZ17I GTA'TAC
Cuts at: 0 2189 9366
Size: 2189 7177
Bsu36I CC'TnA_GG
Cuts at: 0 6995 9366
Size: 6995 2371
BtgI C'CryG_G
Cuts at: 0 3000 4492 4655 8078 9366
Size: 3000 1492 163 3423 1288
Fragments arranged by size:
3423 3000 1492 1288 163
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1559 3099 9366
Size: 1559 1540 6267
Fragments arranged by size:
6267 1559 1540
BtsI GCAGTG_nn'
Cuts at: 0 250 1151 1419 3403 4325 4600 5762 9023
Size: 250 901 268 1984 922 275 1162 3261
Cuts at: 9023 9083 9366
Size: 60 283
Fragments arranged by size:
3261 1984 1162 922 901 283 275 268
250 60
ClaI AT'CG_AT
Cuts at: 0 4300 9366
Size: 4300 5066
DraI TTT'AAA
Cuts at: 0 5495 9366
Size: 5495 3871
DraIII CAC_nnn'GTG
Cuts at: 0 326 524 2530 9366
Size: 326 198 2006 6836
Fragments arranged by size:
6836 2006 326 198
DrdI GACnn_nn'nnGTC
Cuts at: 0 805 9366
Size: 805 8561
EaeI y'GGCC_r
Cuts at: 0 70 1647 3888 4218 8532 9366
Size: 70 1577 2241 330 4314 834
Fragments arranged by size:
4314 2241 1577 834 330 70
EagI C'GGCC_G
Cuts at: 0 70 9366
Size: 70 9296
EarI CTCTTCn'nnn_
Cuts at: 0 1829 2038 2933 7130 7857 9366
Size: 1829 209 895 4197 727 1509
Fragments arranged by size:
4197 1829 1509 895 727 209
EcoICRI GAG'CTC
Cuts at: 0 2102 2697 6171 7176 7614 9114 9366
Size: 2102 595 3474 1005 438 1500 252
Fragments arranged by size:
3474 2102 1500 1005 595 438 252
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 671 1008 1070 1176 1770 1846 1958 2076
Size: 671 337 62 106 594 76 112 118
Cuts at: 2076 2330 2423 2594 2987 3548 3583 4100 4580
Size: 254 93 171 393 561 35 517 480
Cuts at: 4580 4670 4786 5134 5240 5896 5907 6214 6431
Size: 90 116 348 106 656 11 307 217
Cuts at: 6431 6656 6779 7486 7588 8153 8457 8763 8942
Size: 225 123 707 102 565 304 306 179
Cuts at: 8942 9292 9322 9366
Size: 350 30 44
Fragments arranged by size:
707 671 656 594 565 561 517 480
393 350 348 337 307 306 304 254
225 217 179 171 123 118 116 112
106 106 102 93 90 76 62 44
35 30 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2390 4625 5734 8039 9366
Size: 2390 2235 1109 2305 1327
Fragments arranged by size:
2390 2305 2235 1327 1109
EcoO109I rG'GnC_Cy
Cuts at: 0 18 184 2861 4881 5361 5872 8751 9321
Size: 18 166 2677 2020 480 511 2879 570
Cuts at: 9321 9366
Size: 45
Fragments arranged by size:
2879 2677 2020 570 511 480 166 45
18
EcoRI G'AATT_C
Cuts at: 0 5038 7286 8576 8899 9366
Size: 5038 2248 1290 323 467
Fragments arranged by size:
5038 2248 1290 467 323
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 5926 8596 9366
Size: 5926 2670 770
Fragments arranged by size:
5926 2670 770
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 759 791 1112 1144 1344 1376 1669 1701
Size: 759 32 321 32 200 32 293 32
Cuts at: 1701 2492 2524 2629 2661 3663 3695 3828 3860
Size: 791 32 105 32 1002 32 133 32
Cuts at: 3860 5178 5210 5614 5646 5653 5685 6616 6648
Size: 1318 32 404 32 7 32 931 32
Cuts at: 6648 6777 6809 6971 7003 7198 7230 8229 8261
Size: 129 32 162 32 195 32 999 32
Cuts at: 8261 8928 8960 9245 9277 9366
Size: 667 32 285 32 89
Fragments arranged by size:
1318 1002 999 931 791 759 667 404
321 293 285 200 195 162 133 129
105 89 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 7
HincII GTy'rAC
Cuts at: 0 1805 2109 2235 2413 4318 6516 9366
Size: 1805 304 126 178 1905 2198 2850
Fragments arranged by size:
2850 2198 1905 1805 304 178 126
HindIII A'AGCT_T
Cuts at: 0 5653 5777 5919 7975 9366
Size: 5653 124 142 2056 1391
Fragments arranged by size:
5653 2056 1391 142 124
HpaI GTT'AAC
Cuts at: 0 2109 2235 9366
Size: 2109 126 7131
Fragments arranged by size:
7131 2109 126
KpnI G_GTAC'C
Cuts at: 0 6690 7074 9366
Size: 6690 384 2292
Fragments arranged by size:
6690 2292 384
MfeI C'AATT_G
Cuts at: 0 1032 1253 3892 7310 7628 8324 8363 9366
Size: 1032 221 2639 3418 318 696 39 1003
Fragments arranged by size:
3418 2639 1032 1003 696 318 221 39
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 828 6609 6621 8351 8624 9177 9366
Size: 828 5781 12 1730 273 553 189
Fragments arranged by size:
5781 1730 828 553 273 189 12
MscI TGG'CCA
Cuts at: 0 3890 8534 9366
Size: 3890 4644 832
Fragments arranged by size:
4644 3890 832
MslI CAynn'nnrTG
Cuts at: 0 1657 1949 2271 2339 2595 4260 4302 4688
Size: 1657 292 322 68 256 1665 42 386
Cuts at: 4688 5984 7090 7168 7253 9059 9366
Size: 1296 1106 78 85 1806 307
Fragments arranged by size:
1806 1665 1657 1296 1106 386 322 307
292 256 85 78 68 42
MspA1I CmG'CkG
Cuts at: 0 69 84 2122 2158 2633 2993 3494 4120
Size: 69 15 2038 36 475 360 501 626
Cuts at: 4120 4478 4803 4878 4902 6091 6529 8173 8451
Size: 358 325 75 24 1189 438 1644 278
Cuts at: 8451 9172 9366
Size: 721 194
Fragments arranged by size:
2038 1644 1189 721 626 501 475 438
360 358 325 278 194 75 69 36
24 15
NaeI GCC'GGC
Cuts at: 0 1760 3445 9366
Size: 1760 1685 5921
Fragments arranged by size:
5921 1760 1685
NcoI C'CATG_G
Cuts at: 0 8078 9366
Size: 8078 1288
NdeI CA'TA_TG
Cuts at: 0 570 1821 3869 8050 9366
Size: 570 1251 2048 4181 1316
Fragments arranged by size:
4181 2048 1316 1251 570
NgoMIV G'CCGG_C
Cuts at: 0 1758 3443 9366
Size: 1758 1685 5923
Fragments arranged by size:
5923 1758 1685
NotI GC'GGCC_GC
Cuts at: 0 70 9366
Size: 70 9296
NsiI A_TGCA'T
Cuts at: 0 3091 7047 9366
Size: 3091 3956 2319
Fragments arranged by size:
3956 3091 2319
NspI r_CATG'y
Cuts at: 0 173 217 941 1031 1178 1297 4181 4693
Size: 173 44 724 90 147 119 2884 512
Cuts at: 4693 7938 8801 9366
Size: 3245 863 565
Fragments arranged by size:
3245 2884 863 724 565 512 173 147
119 90 44
PasI CC'CwG_GG
Cuts at: 0 220 3331 4149 4239 5031 9366
Size: 220 3111 818 90 792 4335
Fragments arranged by size:
4335 3111 818 792 220 90
PciI A'CATG_T
Cuts at: 0 169 213 937 1174 8797 9366
Size: 169 44 724 237 7623 569
Fragments arranged by size:
7623 724 569 237 169 44
PflMI CCAn_nnn'nTGG
Cuts at: 0 3079 3143 7067 9366
Size: 3079 64 3924 2299
Fragments arranged by size:
3924 3079 2299 64
PfoI T'CCnGG_A
Cuts at: 0 966 5315 8633 9366
Size: 966 4349 3318 733
Fragments arranged by size:
4349 3318 966 733
PmlI CAC'GTG
Cuts at: 0 4640 9366
Size: 4640 4726
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1844 1876 2810 2842 9366
Size: 1844 32 934 32 6524
Fragments arranged by size:
6524 1844 934 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 18 184 2861 5361 5872 9366
Size: 18 166 2677 2500 511 3494
Fragments arranged by size:
3494 2677 2500 511 166 18
PshAI GACnn'nnGTC
Cuts at: 0 1490 2477 8120 9366
Size: 1490 987 5643 1246
Fragments arranged by size:
5643 1490 1246 987
PspOMI G'GGCC_C
Cuts at: 0 4880 9366
Size: 4880 4486
PspXI vC'TCGA_Gb
Cuts at: 0 3953 9366
Size: 3953 5413
PstI C_TGCA'G
Cuts at: 0 1414 3187 3490 3608 5241 5769 8259 9366
Size: 1414 1773 303 118 1633 528 2490 1107
Fragments arranged by size:
2490 1773 1633 1414 1107 528 303 118
PvuII CAG'CTG
Cuts at: 0 2158 2633 3494 4120 4902 6091 6529 8173
Size: 2158 475 861 626 782 1189 438 1644
Cuts at: 8173 8451 9172 9366
Size: 278 721 194
Fragments arranged by size:
2158 1644 1189 861 782 721 626 475
438 278 194
SacI G_AGCT'C
Cuts at: 0 2104 2699 6173 7178 7616 9116 9366
Size: 2104 595 3474 1005 438 1500 250
Fragments arranged by size:
3474 2104 1500 1005 595 438 250
SapI GCTCTTCn'nnn_
Cuts at: 0 7130 9366
Size: 7130 2236
ScaI AGT'ACT
Cuts at: 0 4513 9366
Size: 4513 4853
SexAI A'CCwGG_T
Cuts at: 0 2670 4212 9131 9366
Size: 2670 1542 4919 235
Fragments arranged by size:
4919 2670 1542 235
SfcI C'TryA_G
Cuts at: 0 1410 1488 2159 3183 3378 3486 3604 5237
Size: 1410 78 671 1024 195 108 118 1633
Cuts at: 5237 5765 6587 6935 8255 8709 9366
Size: 528 822 348 1320 454 657
Fragments arranged by size:
1633 1410 1320 1024 822 671 657 528
454 348 195 118 108 78
SmaI CCC'GGG
Cuts at: 0 5059 5284 9366
Size: 5059 225 4082
Fragments arranged by size:
5059 4082 225
SmlI C'TyrA_G
Cuts at: 0 1893 3757 3815 3953 5114 6036 6355 7434
Size: 1893 1864 58 138 1161 922 319 1079
Cuts at: 7434 9217 9366
Size: 1783 149
Fragments arranged by size:
1893 1864 1783 1161 1079 922 319 149
138 58
SphI G_CATG'C
Cuts at: 0 1031 4181 4693 9366
Size: 1031 3150 512 4673
Fragments arranged by size:
4673 3150 1031 512
SspI AAT'ATT
Cuts at: 0 543 3937 5790 6507 7474 8028 9366
Size: 543 3394 1853 717 967 554 1338
Fragments arranged by size:
3394 1853 1338 967 717 554 543
StuI AGG'CCT
Cuts at: 0 47 5125 7750 8094 9366
Size: 47 5078 2625 344 1272
Fragments arranged by size:
5078 2625 1272 344 47
StyI C'CwwG_G
Cuts at: 0 1222 2218 3200 3620 7720 8078 9317 9366
Size: 1222 996 982 420 4100 358 1239 49
Fragments arranged by size:
4100 1239 1222 996 982 420 358 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 819 1946 3150 3543 4948 8515 9366
Size: 819 1127 1204 393 1405 3567 851
Fragments arranged by size:
3567 1405 1204 1127 851 819 393
TatI w'GTAC_w
Cuts at: 0 194 1112 1177 3268 4511 6150 9366
Size: 194 918 65 2091 1243 1639 3216
Fragments arranged by size:
3216 2091 1639 1243 918 194 65
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 654 686 1983 2015 2420 2452 2970 3002
Size: 654 32 1297 32 405 32 518 32
Cuts at: 3002 4236 4268 4473 4505 6975 7007 8855 8887
Size: 1234 32 205 32 2470 32 1848 32
Cuts at: 8887 9366
Size: 479
Fragments arranged by size:
2470 1848 1297 1234 654 518 479 405
205 32 32 32 32 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 4621 6223 9366
Size: 4621 1602 3143
Fragments arranged by size:
4621 3143 1602
XbaI T'CTAG_A
Cuts at: 0 5196 6810 9366
Size: 5196 1614 2556
Fragments arranged by size:
5196 2556 1614
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1153 2797 3585 6184 9366
Size: 1153 1644 788 2599 3182
Fragments arranged by size:
3182 2599 1644 1153 788
XhoI C'TCGA_G
Cuts at: 0 3953 5114 9366
Size: 3953 1161 4252
Fragments arranged by size:
4252 3953 1161
XmaI C'CCGG_G
Cuts at: 0 5057 5282 9366
Size: 5057 225 4084
Fragments arranged by size:
5057 4084 225
XmnI GAAnn'nnTTC
Cuts at: 0 1856 5147 5246 5920 6726 7110 7224 7270
Size: 1856 3291 99 674 806 384 114 46
Cuts at: 7270 8576 9366
Size: 1306 790
Fragments arranged by size:
3291 1856 1306 806 790 674 384 114
99 46
ZraI GAC'GTC
Cuts at: 0 2304 6788 9366
Size: 2304 4484 2578
Fragments arranged by size:
4484 2578 2304
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
DraI DraIII DrdI EaeI EagI EarI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI Hin4I HincII HindIII HpaI
KpnI MfeI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NotI NsiI NspI PasI PciI PflMI
PfoI PmlI PpiI PpuMI PshAI PspOMI PspXI PstI
PvuII SacI SapI ScaI SexAI SfcI SmaI SmlI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AscI AsiSI BaeI BbeI BmtI BsiWI
CspCI EciI EcoRV FseI FspI FspAI HaeII KasI
MluI NarI NheI NruI PacI PmeI PsiI PsrI
PvuI RsrII SacII SalI SanDI SbfI SfiI SfoI
SgfI SgrAI SnaBI SpeI SrfI SwaI