################################## # NO CUT ENZYMES ( 100 enzymes) # ################################## Acc65I AccI AccIII AclI AflII AgeI AhdI AocI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglII BlnI BsaAI BsaBI BseAI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI Eam1105I Eco81I EcoNI EcoO65I EcoRV FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI MamI MfeI MroI MseI MunI NarI NdeI NheI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI ScaI SfoI SfuI SmaI SnaBI SpeI SspBI SspI SwaI Tru9I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AcsI 1959 GAATTC [GA]^AATT[CT] AflIII 590 ACGCGT A^C[GA][CT]GT Alw44I 1480 GTGCAC G^TGCAC Aor51HI 1766 AGCGCT AGC^GCT ApaLI 1480 GTGCAC G^TGCAC ApoI 1959 GAATTC [GA]^AATT[CT] AspI 1500 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC BfaI 437 CTAG C^TAG BlpI 1769 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1769 GCTCAGC GC^T[ACGT]AGC BsaI 497 GAGACC GGTCTC(1/5) BsaWI 1818 TCCGGT [AT]^CCGG[AT] BsiWI 587 CGTACG C^GTACG BsmI 1756 GAATGC GAATGC(1/-1) BspMI 1734 GCAGGT ACCTGC(4/8) BssHII 93 GCGCGC G^CGCGC BstXI 1541 CCAAAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 1769 GCTCAGC GC^T[ACGT]AGC DraIII 409 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1870 GACTCCAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 2043 GAAGAG CTCTTC(1/4) Eco47III 1766 AGCGCT AGC^GCT EcoRI 1959 GAATTC G^AATTC EcoT22I 140 ATGCAT ATGCA^T Ksp632I 2043 GAAGAG CTCTTC(1/4) MaeI 437 CTAG C^TAG MluI 590 ACGCGT A^CGCGT MslI 1881 CATTTTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 610 CCATGG C^CATGG NotI 1715 GCGGCCGC GC^GGCCGC NsiI 140 ATGCAT ATGCA^T NspI 1577 GCATGC [GA]CATG^[CT] PflFI 1500 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 773 GACCCGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SexAI 1475 ACCAGGT A^CC[AT]GGT SfiI 930 GGCCATCGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmlI 1042 CTCAAG C^T[CT][GA]AG SphI 1577 GCATGC GCATG^C Tsp509I 1960 AATT ^AATT Tth111I 1500 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AatII 427 GACGTC GACGT^C 603 GACGTC AlwNI 1153 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG 1777 CAGGAGCTG AspHI 1480 GTGCAC G[AT]GC[AT]^C 1803 GAGCAC AvaI 258 CTCGGG C^[CT]CG[GA]G 649 CCCGAG BalI 1851 TGGCCA TGG^CCA 1905 TGGCCA BsiHKAI 1480 GTGCAC G[AT]GC[AT]^C 1803 GAGCAC BsmBI 1327 GAGACG CGTCTC(1/5) 1648 GAGACG BsoBI 258 CTCGGG C^[CT]CG[GA]G 649 CCCGAG BsrBI 169 CCGCTC CCGCTC(-3/-3) 762 CCGCTC BstYI 1553 AGATCC [GA]^GATC[CT] 1976 AGATCC EcoT14I 610 CCATGG C^C[AT][AT]GG 1874 CCAAGG HaeII 1038 GGCGCT [GA]GCGC^[CT] 1766 AGCGCT MflI 1553 AGATCC [GA]^GATC[CT] 1976 AGATCC MluNI 1851 TGGCCA TGG^CCA 1905 TGGCCA MscI 1851 TGGCCA TGG^CCA 1905 TGGCCA PflMI 408 CCACGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1152 CCAGTACCTGG PpuMI 1064 AGGACCT [GA]G^G[AT]CC[CT] 1943 GGGACCT PvuII 1252 CAGCTG CAG^CTG 1384 CAGCTG SbfI 1731 CCTGCAGG CCTGCA^GG 1863 CCTGCAGG SgrAI 96 CGCCGGCG C[GA]^CCGG[CT]G 242 CGCCGGCG Sse8387I 1731 CCTGCAGG CCTGCA^GG 1863 CCTGCAGG StyI 610 CCATGG C^C[AT][AT]GG 1874 CCAAGG TaqI 359 TCGA T^CGA 1754 TCGA TfiI 1604 GAATC G^A[AT]TC 1992 GAATC Tsp45I 736 GTCAC ^GT[GC]AC 883 GTGAC TthHB8I 359 TCGA T^CGA 1754 TCGA Van91I 408 CCACGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1152 CCAGTACCTGG XhoII 1553 AGATCC [GA]^GATC[CT] 1976 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 90 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI ApaI AvaII BanI BanII BbsI BbvI BcnI BglI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsgI BsiEI BsiYI BslI BsmAI BsmFI Bsp1286I BsrFI BsrI BssKI BssSI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EclXI Eco52I EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KspI MaeII MaeIII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV PleI PstI RsaI SacII Sau3AI Sau96I ScrFI SfaNI SfcI StuI TseI TspRI