(Linear) (Six-base) MAPSORT of: KIEE2485.seq Check: 3569 from: 1 to: 2148 KIEE2485.orf With 182 enzymes: SgfI * October 10, 2013 10:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1725 2148 Size: 1725 423 AatII G_ACGT'C Cuts at: 0 431 607 2148 Size: 431 176 1541 Fragments arranged by size: 1541 431 176 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 199 2148 Size: 199 1949 AfeI AGC'GCT Cuts at: 0 1768 2148 Size: 1768 380 AflIII A'CryG_T Cuts at: 0 590 2148 Size: 590 1558 AlwNI CAG_nnn'CTG Cuts at: 0 1158 1782 2148 Size: 1158 624 366 Fragments arranged by size: 1158 624 366 ApaI G_GGCC'C Cuts at: 0 86 290 336 2148 Size: 86 204 46 1812 Fragments arranged by size: 1812 204 86 46 ApaLI G'TGCA_C Cuts at: 0 1480 2148 Size: 1480 668 ApoI r'AATT_y Cuts at: 0 1959 2148 Size: 1959 189 AvaI C'yCGr_G Cuts at: 0 258 649 2148 Size: 258 391 1499 Fragments arranged by size: 1499 391 258 BanI G'GyrC_C Cuts at: 0 103 757 1164 2148 Size: 103 654 407 984 Fragments arranged by size: 984 654 407 103 BanII G_rGCy'C Cuts at: 0 86 290 336 517 1055 1228 1320 2148 Size: 86 204 46 181 538 173 92 828 Fragments arranged by size: 828 538 204 181 173 92 86 46 BbsI GAAGACnn'nnnn_ Cuts at: 0 51 431 1112 1504 2148 Size: 51 380 681 392 644 Fragments arranged by size: 681 644 392 380 51 BfrBI ATG'CAT Cuts at: 0 142 2148 Size: 142 2006 BglI GCCn_nnn'nGGC Cuts at: 0 812 937 1354 2148 Size: 812 125 417 794 Fragments arranged by size: 812 794 417 125 BlpI GC'TnA_GC Cuts at: 0 1770 2148 Size: 1770 378 Bme1580I G_kGCm'C Cuts at: 0 86 290 336 762 1484 2148 Size: 86 204 46 426 722 664 Fragments arranged by size: 722 664 426 204 86 46 BmgBI CAC'GTC Cuts at: 0 1701 2019 2148 Size: 1701 318 129 Fragments arranged by size: 1701 318 129 BmrI ACTGGGnnnn_n' Cuts at: 0 909 2148 Size: 909 1239 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 384 576 2148 Size: 384 192 1572 Fragments arranged by size: 1572 384 192 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 364 505 753 1039 1570 2148 Size: 364 141 248 286 531 578 Fragments arranged by size: 578 531 364 286 248 141 Bpu10I CC'TnA_GC Cuts at: 0 511 1775 2148 Size: 511 1264 373 Fragments arranged by size: 1264 511 373 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1027 2148 Size: 1027 1121 BsaI GGTCTCn'nnnn_ Cuts at: 0 491 2148 Size: 491 1657 BsaHI Gr'CG_yC Cuts at: 0 428 604 989 1357 2148 Size: 428 176 385 368 791 Fragments arranged by size: 791 428 385 368 176 BsaWI w'CCGG_w Cuts at: 0 1818 2148 Size: 1818 330 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 289 319 697 727 974 1004 2148 Size: 289 30 378 30 247 30 1144 Fragments arranged by size: 1144 378 289 247 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 83 297 549 667 756 757 1104 2148 Size: 83 214 252 118 89 1 347 1044 Fragments arranged by size: 1044 347 252 214 118 89 83 1 BseYI C'CCAG_C Cuts at: 0 478 672 690 753 1711 1763 2148 Size: 478 194 18 63 958 52 385 Fragments arranged by size: 958 478 385 194 63 52 18 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 666 1315 1402 2148 Size: 666 649 87 746 Fragments arranged by size: 746 666 649 87 BsiEI CG_ry'CG Cuts at: 0 816 1091 1364 1449 1719 1823 2148 Size: 816 275 273 85 270 104 325 Fragments arranged by size: 816 325 275 273 270 104 85 BsiHKAI G_wGCw'C Cuts at: 0 1484 1807 2148 Size: 1484 323 341 Fragments arranged by size: 1484 341 323 BsiWI C'GTAC_G Cuts at: 0 587 2148 Size: 587 1561 BsmI GAATG_Cn' Cuts at: 0 1762 2148 Size: 1762 386 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1321 1642 2148 Size: 1321 321 506 Fragments arranged by size: 1321 506 321 Bsp1286I G_dGCh'C Cuts at: 0 86 290 336 517 762 1055 1228 1320 Size: 86 204 46 181 245 293 173 92 Cuts at: 1320 1484 1807 2148 Size: 164 323 341 Fragments arranged by size: 341 323 293 245 204 181 173 164 92 86 46 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1725 2148 Size: 1725 423 BsrBI CCG'CTC Cuts at: 0 171 764 2148 Size: 171 593 1384 Fragments arranged by size: 1384 593 171 BsrFI r'CCGG_y Cuts at: 0 97 195 243 815 2148 Size: 97 98 48 572 1333 Fragments arranged by size: 1333 572 98 97 48 BssHII G'CGCG_C Cuts at: 0 93 2148 Size: 93 2055 BssSI C'ACGA_G Cuts at: 0 369 504 894 1519 2148 Size: 369 135 390 625 629 Fragments arranged by size: 629 625 390 369 135 BstXI CCAn_nnnn'nTGG Cuts at: 0 1548 2148 Size: 1548 600 BstYI r'GATC_y Cuts at: 0 1553 1976 2148 Size: 1553 423 172 Fragments arranged by size: 1553 423 172 BtgI C'CryG_G Cuts at: 0 176 290 458 610 641 666 700 871 Size: 176 114 168 152 31 25 34 171 Cuts at: 871 2148 Size: 1277 Fragments arranged by size: 1277 176 171 168 152 114 34 31 25 BtsI GCAGTG_nn' Cuts at: 0 685 2148 Size: 685 1463 DraIII CAC_nnn'GTG Cuts at: 0 414 2148 Size: 414 1734 DrdI GACnn_nn'nnGTC Cuts at: 0 1876 2148 Size: 1876 272 EaeI y'GGCC_r Cuts at: 0 312 461 518 813 929 938 1088 1361 Size: 312 149 57 295 116 9 150 273 Cuts at: 1361 1716 1851 1905 2148 Size: 355 135 54 243 Fragments arranged by size: 355 312 295 273 243 150 149 135 116 57 54 9 EagI C'GGCC_G Cuts at: 0 813 1088 1361 1716 2148 Size: 813 275 273 355 432 Fragments arranged by size: 813 432 355 275 273 EarI CTCTTCn'nnn_ Cuts at: 0 2038 2148 Size: 2038 110 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 262 1456 2148 Size: 262 1194 692 Fragments arranged by size: 1194 692 262 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 199 364 505 753 1039 1570 2148 Size: 199 165 141 248 286 531 578 Fragments arranged by size: 578 531 286 248 199 165 141 EcoO109I rG'GnC_Cy Cuts at: 0 82 332 333 677 1065 1944 2148 Size: 82 250 1 344 388 879 204 Fragments arranged by size: 879 388 344 250 204 82 1 EcoRI G'AATT_C Cuts at: 0 1959 2148 Size: 1959 189 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1158 2148 Size: 1158 990 HaeII r_GCGC'y Cuts at: 0 1042 1770 2148 Size: 1042 728 378 Fragments arranged by size: 1042 728 378 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 241 273 974 1006 1099 1131 1361 1393 Size: 241 32 701 32 93 32 230 32 Cuts at: 1393 2148 Size: 755 Fragments arranged by size: 755 701 241 230 93 32 32 32 32 MluI A'CGCG_T Cuts at: 0 590 2148 Size: 590 1558 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 173 596 1559 1649 1769 2148 Size: 173 423 963 90 120 379 Fragments arranged by size: 963 423 379 173 120 90 MscI TGG'CCA Cuts at: 0 1853 1907 2148 Size: 1853 54 241 Fragments arranged by size: 1853 241 54 MslI CAynn'nnrTG Cuts at: 0 1885 2148 Size: 1885 263 MspA1I CmG'CkG Cuts at: 0 118 292 668 702 873 1254 1386 1413 Size: 118 174 376 34 171 381 132 27 Cuts at: 1413 1715 2148 Size: 302 433 Fragments arranged by size: 433 381 376 302 174 171 132 118 34 27 NaeI GCC'GGC Cuts at: 0 99 197 245 817 2148 Size: 99 98 48 572 1331 Fragments arranged by size: 1331 572 99 98 48 NcoI C'CATG_G Cuts at: 0 610 2148 Size: 610 1538 NgoMIV G'CCGG_C Cuts at: 0 97 195 243 815 2148 Size: 97 98 48 572 1333 Fragments arranged by size: 1333 572 98 97 48 NotI GC'GGCC_GC Cuts at: 0 1716 2148 Size: 1716 432 NsiI A_TGCA'T Cuts at: 0 144 2148 Size: 144 2004 NspI r_CATG'y Cuts at: 0 1581 2148 Size: 1581 567 PflMI CCAn_nnn'nTGG Cuts at: 0 414 1158 2148 Size: 414 744 990 Fragments arranged by size: 990 744 414 PfoI T'CCnGG_A Cuts at: 0 535 1547 2148 Size: 535 1012 601 Fragments arranged by size: 1012 601 535 PpuMI rG'GwC_Cy Cuts at: 0 1065 1944 2148 Size: 1065 879 204 Fragments arranged by size: 1065 879 204 PshAI GACnn'nnGTC Cuts at: 0 777 2148 Size: 777 1371 PspOMI G'GGCC_C Cuts at: 0 82 286 332 2148 Size: 82 204 46 1816 Fragments arranged by size: 1816 204 82 46 PstI C_TGCA'G Cuts at: 0 1424 1736 1868 2148 Size: 1424 312 132 280 Fragments arranged by size: 1424 312 280 132 PvuII CAG'CTG Cuts at: 0 1254 1386 2148 Size: 1254 132 762 Fragments arranged by size: 1254 762 132 SacII CC_GC'GG Cuts at: 0 293 669 703 874 2148 Size: 293 376 34 171 1274 Fragments arranged by size: 1274 376 293 171 34 SbfI CC_TGCA'GG Cuts at: 0 1736 1868 2148 Size: 1736 132 280 Fragments arranged by size: 1736 280 132 SexAI A'CCwGG_T Cuts at: 0 1475 2148 Size: 1475 673 SfcI C'TryA_G Cuts at: 0 492 1420 1732 1864 2148 Size: 492 928 312 132 284 Fragments arranged by size: 928 492 312 284 132 SfiI GGCCn_nnn'nGGCC Cuts at: 0 937 2148 Size: 937 1211 SgrAI Cr'CCGG_yG Cuts at: 0 97 243 2148 Size: 97 146 1905 Fragments arranged by size: 1905 146 97 SmlI C'TyrA_G Cuts at: 0 1042 2148 Size: 1042 1106 SphI G_CATG'C Cuts at: 0 1581 2148 Size: 1581 567 StuI AGG'CCT Cuts at: 0 324 490 556 2148 Size: 324 166 66 1592 Fragments arranged by size: 1592 324 166 66 StyI C'CwwG_G Cuts at: 0 610 1874 2148 Size: 610 1264 274 Fragments arranged by size: 1264 610 274 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 745 901 928 2148 Size: 745 156 27 1220 Fragments arranged by size: 1220 745 156 27 TatI w'GTAC_w Cuts at: 0 800 917 2148 Size: 800 117 1231 Fragments arranged by size: 1231 800 117 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 32 697 729 2064 2096 2148 Size: 32 665 32 1335 32 52 Fragments arranged by size: 1335 665 52 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1503 2148 Size: 1503 645 ZraI GAC'GTC Cuts at: 0 429 605 2148 Size: 429 176 1543 Fragments arranged by size: 1543 429 176 Enzymes that do cut: AarI AatII AcuI AfeI AflIII AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbsI BfrBI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspMI BsrBI BsrFI BssHII BssSI BstXI BstYI BtgI BtsI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI HaeII Hin4I MluI MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NotI NsiI NspI PflMI PfoI PpuMI PshAI PspOMI PstI PvuII SacII SbfI SexAI SfcI SfiI SgrAI SmlI SphI StuI StyI TaqII TatI TstI Tth111I ZraI Enzymes that do not cut: AccI Acc65I AclI AflII AgeI AhdI AleI AlfI AloI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI BglII BmtI BsaAI BsaBI BspEI BspHI BsrDI BsrGI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI EcoICRI EcoNI EcoRV FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI NarI NdeI NheI NruI PacI PasI PciI PmeI PmlI PpiI PsiI PspXI PsrI PvuI RsrII SacI SalI SanDI SapI ScaI SfoI SgfI SmaI SnaBI SpeI SrfI SspI SwaI XbaI XcmI XhoI XmaI XmnI