Restriction map for pFN21AE2485
 (Linear) (Six-base) MAPSORT of: KIEE2485.seq  Check: 3569  from: 1  to: 2148



KIEE2485.orf

 With 182 enzymes: SgfI * 

                        October 10, 2013 10:36  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1725    2148 
   Size:       1725     423
 
AatII G_ACGT'C

Cuts at:      0     431     607    2148 
   Size:        431     176    1541

  Fragments arranged by size: 

                1541     431     176
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     199    2148 
   Size:        199    1949
 
AfeI AGC'GCT

Cuts at:      0    1768    2148 
   Size:       1768     380
 
AflIII A'CryG_T

Cuts at:      0     590    2148 
   Size:        590    1558
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1158    1782    2148 
   Size:       1158     624     366

  Fragments arranged by size: 

                1158     624     366
 
ApaI G_GGCC'C

Cuts at:      0      86     290     336    2148 
   Size:         86     204      46    1812

  Fragments arranged by size: 

                1812     204      86      46
 
ApaLI G'TGCA_C

Cuts at:      0    1480    2148 
   Size:       1480     668
 
ApoI r'AATT_y

Cuts at:      0    1959    2148 
   Size:       1959     189
 
AvaI C'yCGr_G

Cuts at:      0     258     649    2148 
   Size:        258     391    1499

  Fragments arranged by size: 

                1499     391     258
 
BanI G'GyrC_C

Cuts at:      0     103     757    1164    2148 
   Size:        103     654     407     984

  Fragments arranged by size: 

                 984     654     407     103
 
BanII G_rGCy'C

Cuts at:      0      86     290     336     517    1055    1228    1320    2148 
   Size:         86     204      46     181     538     173      92     828

  Fragments arranged by size: 

                 828     538     204     181     173      92      86      46
            
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      51     431    1112    1504    2148 
   Size:         51     380     681     392     644

  Fragments arranged by size: 

                 681     644     392     380      51
 
BfrBI ATG'CAT

Cuts at:      0     142    2148 
   Size:        142    2006
 
BglI GCCn_nnn'nGGC

Cuts at:      0     812     937    1354    2148 
   Size:        812     125     417     794

  Fragments arranged by size: 

                 812     794     417     125
 
BlpI GC'TnA_GC

Cuts at:      0    1770    2148 
   Size:       1770     378
 
Bme1580I G_kGCm'C

Cuts at:      0      86     290     336     762    1484    2148 
   Size:         86     204      46     426     722     664

  Fragments arranged by size: 

                 722     664     426     204      86      46
 
BmgBI CAC'GTC

Cuts at:      0    1701    2019    2148 
   Size:       1701     318     129

  Fragments arranged by size: 

                1701     318     129
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     909    2148 
   Size:        909    1239
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     384     576    2148 
   Size:        384     192    1572

  Fragments arranged by size: 

                1572     384     192
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     364     505     753    1039    1570    2148 
   Size:        364     141     248     286     531     578

  Fragments arranged by size: 

                 578     531     364     286     248     141
 
Bpu10I CC'TnA_GC

Cuts at:      0     511    1775    2148 
   Size:        511    1264     373

  Fragments arranged by size: 

                1264     511     373
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1027    2148 
   Size:       1027    1121
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     491    2148 
   Size:        491    1657
 
BsaHI Gr'CG_yC

Cuts at:      0     428     604     989    1357    2148 
   Size:        428     176     385     368     791

  Fragments arranged by size: 

                 791     428     385     368     176
 
BsaWI w'CCGG_w

Cuts at:      0    1818    2148 
   Size:       1818     330
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     289     319     697     727     974    1004    2148 
   Size:        289      30     378      30     247      30    1144

  Fragments arranged by size: 

                1144     378     289     247      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      83     297     549     667     756     757    1104    2148 
   Size:         83     214     252     118      89       1     347    1044

  Fragments arranged by size: 

                1044     347     252     214     118      89      83       1
            
 
BseYI C'CCAG_C

Cuts at:      0     478     672     690     753    1711    1763    2148 
   Size:        478     194      18      63     958      52     385

  Fragments arranged by size: 

                 958     478     385     194      63      52      18
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     666    1315    1402    2148 
   Size:        666     649      87     746

  Fragments arranged by size: 

                 746     666     649      87
 
BsiEI CG_ry'CG

Cuts at:      0     816    1091    1364    1449    1719    1823    2148 
   Size:        816     275     273      85     270     104     325

  Fragments arranged by size: 

                 816     325     275     273     270     104      85
 
BsiHKAI G_wGCw'C

Cuts at:      0    1484    1807    2148 
   Size:       1484     323     341

  Fragments arranged by size: 

                1484     341     323
 
BsiWI C'GTAC_G

Cuts at:      0     587    2148 
   Size:        587    1561
 
BsmI GAATG_Cn'

Cuts at:      0    1762    2148 
   Size:       1762     386
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1321    1642    2148 
   Size:       1321     321     506

  Fragments arranged by size: 

                1321     506     321
 
Bsp1286I G_dGCh'C

Cuts at:      0      86     290     336     517     762    1055    1228    1320 
   Size:         86     204      46     181     245     293     173      92

Cuts at:   1320    1484    1807    2148 
   Size:        164     323     341

  Fragments arranged by size: 

                 341     323     293     245     204     181     173     164
                  92      86      46
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1725    2148 
   Size:       1725     423
 
BsrBI CCG'CTC

Cuts at:      0     171     764    2148 
   Size:        171     593    1384

  Fragments arranged by size: 

                1384     593     171
 
BsrFI r'CCGG_y

Cuts at:      0      97     195     243     815    2148 
   Size:         97      98      48     572    1333

  Fragments arranged by size: 

                1333     572      98      97      48
 
BssHII G'CGCG_C

Cuts at:      0      93    2148 
   Size:         93    2055
 
BssSI C'ACGA_G

Cuts at:      0     369     504     894    1519    2148 
   Size:        369     135     390     625     629

  Fragments arranged by size: 

                 629     625     390     369     135
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1548    2148 
   Size:       1548     600
 
BstYI r'GATC_y

Cuts at:      0    1553    1976    2148 
   Size:       1553     423     172

  Fragments arranged by size: 

                1553     423     172
 
BtgI C'CryG_G

Cuts at:      0     176     290     458     610     641     666     700     871 
   Size:        176     114     168     152      31      25      34     171

Cuts at:    871    2148 
   Size:       1277

  Fragments arranged by size: 

                1277     176     171     168     152     114      34      31
                  25
 
BtsI GCAGTG_nn'

Cuts at:      0     685    2148 
   Size:        685    1463
 
DraIII CAC_nnn'GTG

Cuts at:      0     414    2148 
   Size:        414    1734
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1876    2148 
   Size:       1876     272
 
EaeI y'GGCC_r

Cuts at:      0     312     461     518     813     929     938    1088    1361 
   Size:        312     149      57     295     116       9     150     273

Cuts at:   1361    1716    1851    1905    2148 
   Size:        355     135      54     243

  Fragments arranged by size: 

                 355     312     295     273     243     150     149     135
                 116      57      54       9
 
EagI C'GGCC_G

Cuts at:      0     813    1088    1361    1716    2148 
   Size:        813     275     273     355     432

  Fragments arranged by size: 

                 813     432     355     275     273
 
EarI CTCTTCn'nnn_

Cuts at:      0    2038    2148 
   Size:       2038     110
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     262    1456    2148 
   Size:        262    1194     692

  Fragments arranged by size: 

                1194     692     262
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     199     364     505     753    1039    1570    2148 
   Size:        199     165     141     248     286     531     578

  Fragments arranged by size: 

                 578     531     286     248     199     165     141
 
EcoO109I rG'GnC_Cy

Cuts at:      0      82     332     333     677    1065    1944    2148 
   Size:         82     250       1     344     388     879     204

  Fragments arranged by size: 

                 879     388     344     250     204      82       1
 
EcoRI G'AATT_C

Cuts at:      0    1959    2148 
   Size:       1959     189
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1158    2148 
   Size:       1158     990
 
HaeII r_GCGC'y

Cuts at:      0    1042    1770    2148 
   Size:       1042     728     378

  Fragments arranged by size: 

                1042     728     378
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     241     273     974    1006    1099    1131    1361    1393 
   Size:        241      32     701      32      93      32     230      32

Cuts at:   1393    2148 
   Size:        755

  Fragments arranged by size: 

                 755     701     241     230      93      32      32      32
                  32
 
MluI A'CGCG_T

Cuts at:      0     590    2148 
   Size:        590    1558
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     173     596    1559    1649    1769    2148 
   Size:        173     423     963      90     120     379

  Fragments arranged by size: 

                 963     423     379     173     120      90
 
MscI TGG'CCA

Cuts at:      0    1853    1907    2148 
   Size:       1853      54     241

  Fragments arranged by size: 

                1853     241      54
 
MslI CAynn'nnrTG

Cuts at:      0    1885    2148 
   Size:       1885     263
 
MspA1I CmG'CkG

Cuts at:      0     118     292     668     702     873    1254    1386    1413 
   Size:        118     174     376      34     171     381     132      27

Cuts at:   1413    1715    2148 
   Size:        302     433

  Fragments arranged by size: 

                 433     381     376     302     174     171     132     118
                  34      27
 
NaeI GCC'GGC

Cuts at:      0      99     197     245     817    2148 
   Size:         99      98      48     572    1331

  Fragments arranged by size: 

                1331     572      99      98      48
 
NcoI C'CATG_G

Cuts at:      0     610    2148 
   Size:        610    1538
 
NgoMIV G'CCGG_C

Cuts at:      0      97     195     243     815    2148 
   Size:         97      98      48     572    1333

  Fragments arranged by size: 

                1333     572      98      97      48
 
NotI GC'GGCC_GC

Cuts at:      0    1716    2148 
   Size:       1716     432
 
NsiI A_TGCA'T

Cuts at:      0     144    2148 
   Size:        144    2004
 
NspI r_CATG'y

Cuts at:      0    1581    2148 
   Size:       1581     567
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     414    1158    2148 
   Size:        414     744     990

  Fragments arranged by size: 

                 990     744     414
 
PfoI T'CCnGG_A

Cuts at:      0     535    1547    2148 
   Size:        535    1012     601

  Fragments arranged by size: 

                1012     601     535
 
PpuMI rG'GwC_Cy

Cuts at:      0    1065    1944    2148 
   Size:       1065     879     204

  Fragments arranged by size: 

                1065     879     204
 
PshAI GACnn'nnGTC

Cuts at:      0     777    2148 
   Size:        777    1371
 
PspOMI G'GGCC_C

Cuts at:      0      82     286     332    2148 
   Size:         82     204      46    1816

  Fragments arranged by size: 

                1816     204      82      46
 
PstI C_TGCA'G

Cuts at:      0    1424    1736    1868    2148 
   Size:       1424     312     132     280

  Fragments arranged by size: 

                1424     312     280     132
 
PvuII CAG'CTG

Cuts at:      0    1254    1386    2148 
   Size:       1254     132     762

  Fragments arranged by size: 

                1254     762     132
 
SacII CC_GC'GG

Cuts at:      0     293     669     703     874    2148 
   Size:        293     376      34     171    1274

  Fragments arranged by size: 

                1274     376     293     171      34
 
SbfI CC_TGCA'GG

Cuts at:      0    1736    1868    2148 
   Size:       1736     132     280

  Fragments arranged by size: 

                1736     280     132
 
SexAI A'CCwGG_T

Cuts at:      0    1475    2148 
   Size:       1475     673
 
SfcI C'TryA_G

Cuts at:      0     492    1420    1732    1864    2148 
   Size:        492     928     312     132     284

  Fragments arranged by size: 

                 928     492     312     284     132
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     937    2148 
   Size:        937    1211
 
SgrAI Cr'CCGG_yG

Cuts at:      0      97     243    2148 
   Size:         97     146    1905

  Fragments arranged by size: 

                1905     146      97
 
SmlI C'TyrA_G

Cuts at:      0    1042    2148 
   Size:       1042    1106
 
SphI G_CATG'C

Cuts at:      0    1581    2148 
   Size:       1581     567
 
StuI AGG'CCT

Cuts at:      0     324     490     556    2148 
   Size:        324     166      66    1592

  Fragments arranged by size: 

                1592     324     166      66
 
StyI C'CwwG_G

Cuts at:      0     610    1874    2148 
   Size:        610    1264     274

  Fragments arranged by size: 

                1264     610     274
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     745     901     928    2148 
   Size:        745     156      27    1220

  Fragments arranged by size: 

                1220     745     156      27
 
TatI w'GTAC_w

Cuts at:      0     800     917    2148 
   Size:        800     117    1231

  Fragments arranged by size: 

                1231     800     117
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      32     697     729    2064    2096    2148 
   Size:         32     665      32    1335      32      52

  Fragments arranged by size: 

                1335     665      52      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1503    2148 
   Size:       1503     645
 
ZraI GAC'GTC

Cuts at:      0     429     605    2148 
   Size:        429     176    1543

  Fragments arranged by size: 

                1543     429     176
 

 Enzymes that do cut:

     AarI    AatII     AcuI     AfeI   AflIII    AlwNI     ApaI    ApaLI
     ApoI     AvaI     BanI    BanII     BbsI    BfrBI     BglI     BlpI
 Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspMI    BsrBI    BsrFI   BssHII
    BssSI    BstXI    BstYI     BtgI     BtsI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  Eco57MI EcoO109I    EcoRI     FalI    HaeII
    Hin4I     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NcoI
   NgoMIV     NotI     NsiI     NspI    PflMI     PfoI    PpuMI    PshAI
   PspOMI     PstI    PvuII    SacII     SbfI    SexAI     SfcI     SfiI
    SgrAI     SmlI     SphI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI    AflII     AgeI     AhdI     AleI     AlfI
     AloI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI
    BbvCI     BcgI    BciVI     BclI    BglII     BmtI    BsaAI    BsaBI
    BspEI    BspHI    BsrDI    BsrGI   BstAPI    BstBI   BstEII  BstZ17I
   Bsu36I    BtgZI     ClaI    CspCI     DraI  EcoICRI    EcoNI    EcoRV
     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     NarI     NdeI     NheI     NruI     PacI     PasI     PciI
     PmeI     PmlI     PpiI     PsiI    PspXI     PsrI     PvuI    RsrII
     SacI     SalI    SanDI     SapI     ScaI     SfoI     SgfI     SmaI
    SnaBI     SpeI     SrfI     SspI     SwaI     XbaI     XcmI     XhoI
     XmaI     XmnI
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