################################## # NO CUT ENZYMES ( 206 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstBI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DpnII DraI DraII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT14I EcoT22I FauI FbaI FokI FseI FspI HaeII HaeIII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MboI MfeI MflI MluI MluNI MroI MscI MseI MspA1I MunI MvaI MvnI NaeI NarI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau3AI SbfI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tru9I Tsp509I Tth111I TthHB8I Van91I XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AciI 71 CCGC CCGC(-3/-1) AfaI 326 GTAC GT^AC BanI 136 GGTGCC G^G[CT][GA]CC BbsI 204 GAAGAC GAAGAC(2/6) BcnI 133 CCGGG CC^[GC]GG BfaI 173 CTAG C^TAG BmyI 137 GTGCCC G[AGT]GC[ACT]^C BpuAI 204 GAAGAC GAAGAC(2/6) BsgI 82 GTGCAG GTGCAG(16/14) BsmI 97 GAATGC GAATGC(1/-1) Bsp1286I 137 GTGCCC G[AGT]GC[ACT]^C BsrBI 71 CCGCTC CCGCTC(-3/-3) BssKI 133 CCGGG ^CC[ACGT]GG BstAPI 155 GCATGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DraIII 309 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 121 CTCTTC CTCTTC(1/4) HapII 133 CCGG C^CGG HinfI 238 GAGTC G^A[ACGT]TC HpaII 133 CCGG C^CGG HphI 230 TCACC GGTGA(8/7) Ksp632I 121 CTCTTC CTCTTC(1/4) MaeI 173 CTAG C^TAG MaeII 227 ACGT A^CGT MnlI 233 CCTC CCTC(7/6) MslI 276 CACAGTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 133 CCGG C^CGG NciI 133 CCGGG CC^[GC]GG NlaIV 136 GGTGCC GG[ACGT]^[ACGT]CC NspI 57 GCATGT [GA]CATG^[CT] PleI 238 GAGTC GAGTC(4/5) PstI 321 CTGCAG CTGCA^G RsaI 326 GTAC GT^AC ScaI 325 AGTACT AGT^ACT ScrFI 133 CCGGG CC^[ACGT]GG XbaI 172 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 10 enzymes) # ################################### AluI 24 AGCT AG^CT 255 AGCT AvaII 197 GGTCC G^G[AT]CC 216 GGTCC Cac8I 101 GCACGC GC[ACGT]^[ACGT]GC 313 GCGTGC Cfr13I 197 GGTCC G^G[ACGT]CC 216 GGTCC DdeI 193 CTAAG C^T[ACGT]AG 234 CTCAG MboII 204 GAAGA GAAGA(8/7) 122 TCTTC MwoI 92 GCCCAGAATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 155 GCATGGGCTGC NlaIII 58 CATG CATG^ 156 CATG Sau96I 197 GGTCC G^G[ACGT]CC 216 GGTCC TspRI 151 CAGTG CA[GC]TG[ACGT][ACGT]^ 319 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 7 enzymes) # ######################################### BbvI Fnu4HI ItaI MaeIII SfcI TseI Tsp45I