(Circular) (Six-base) MAPSORT of: pFN21AE5140.seq Check: 8178 from: 1 to: 5053 pFN21AE5140 5053 bp With 182 enzymes: SgfI * January 30, 2014 17:13 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2863 278 Size: 53 83 186 936 1327 2468 Fragments arranged by size: 2468 1327 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2396 1937 Size: 459 4594 Acc65I G'GTAC_C Cuts at: 2374 2374 Size: 5053 AclI AA'CG_TT Cuts at: 1563 3180 3553 1563 Size: 1617 373 3063 Fragments arranged by size: 3063 1617 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2138 3116 4500 4768 1632 Size: 506 978 1384 268 1917 Fragments arranged by size: 1917 1384 978 506 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4856 AflIII A'CryG_T Cuts at: 1203 3952 4929 1203 Size: 2749 977 1327 Fragments arranged by size: 2749 1327 977 AgeI A'CCGG_T Cuts at: 3884 3884 Size: 5053 AhdI GACnn_n'nnGTC Cuts at: 3782 3782 Size: 5053 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5053 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5053 AlwNI CAG_nnn'CTG Cuts at: 2796 4368 2796 Size: 1572 3481 ApaLI G'TGCA_C Cuts at: 3110 4266 3110 Size: 1156 3897 ApoI r'AATT_y Cuts at: 1493 2362 2588 2740 4849 1493 Size: 869 226 152 2109 1697 Fragments arranged by size: 2109 1697 869 226 152 AseI AT'TA_AT Cuts at: 160 1032 3607 160 Size: 872 2575 1606 Fragments arranged by size: 2575 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5053 AvaI C'yCGr_G Cuts at: 1870 1943 2378 1870 Size: 73 435 4545 Fragments arranged by size: 4545 435 73 BamHI G'GATC_C Cuts at: 2383 2750 2383 Size: 367 4686 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2141 2374 3829 618 Size: 325 217 627 354 233 1455 1842 Fragments arranged by size: 1842 1455 627 354 325 233 217 BanII G_rGCy'C Cuts at: 1391 2372 1391 Size: 981 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2216 928 Size: 971 317 3765 Fragments arranged by size: 3765 971 317 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3244 3278 1903 Size: 34 1307 34 3678 Fragments arranged by size: 3678 1307 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2945 4155 2945 Size: 1210 3843 BclI T'GATC_A Cuts at: 1548 1548 Size: 5053 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3664 136 Size: 107 122 71 631 605 1992 1525 Fragments arranged by size: 1992 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5048 5048 Size: 5053 BlpI GC'TnA_GC Cuts at: 2470 2470 Size: 5053 Bme1580I G_kGCm'C Cuts at: 1790 2146 3114 4270 1790 Size: 356 968 1156 2573 Fragments arranged by size: 2573 1156 968 356 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5053 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2784 3742 442 Size: 416 53 83 399 24 1367 958 1753 Fragments arranged by size: 1753 1367 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5053 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3713 1239 Size: 2474 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3178 4043 4341 3178 Size: 865 298 3890 Fragments arranged by size: 3890 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3716 882 Size: 617 2217 2219 Fragments arranged by size: 2219 2217 617 BsaAI yAC'GTr Cuts at: 493 1204 2844 493 Size: 711 1640 2702 Fragments arranged by size: 2702 1640 711 BsaBI GATnn'nnATC Cuts at: 5047 5047 Size: 5053 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2860 3242 3877 275 Size: 53 83 186 936 1327 382 635 1451 Fragments arranged by size: 1451 1327 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3486 3884 4158 4305 4716 3486 Size: 398 274 147 411 3823 Fragments arranged by size: 3823 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4828 Fragments arranged by size: 4828 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5053 BseYI C'CCAG_C Cuts at: 2133 4256 4752 2133 Size: 2123 496 2434 Fragments arranged by size: 2434 2123 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2108 1163 Size: 573 372 4108 Fragments arranged by size: 4108 573 372 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2503 3264 4292 1540 Size: 275 186 502 761 1028 2301 Fragments arranged by size: 2301 1028 761 502 275 186 BsiHKAI G_wGCw'C Cuts at: 3114 3199 4270 3114 Size: 85 1071 3897 Fragments arranged by size: 3897 1071 85 BsmI GAATG_Cn' Cuts at: 2108 2564 2657 2108 Size: 456 93 4504 Fragments arranged by size: 4504 456 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2146 2372 3114 3199 4270 1391 Size: 399 356 226 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 356 226 85 BspEI T'CCGG_A Cuts at: 4716 4716 Size: 5053 BspHI T'CATG_A Cuts at: 2942 4672 2942 Size: 1730 3323 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2408 844 Size: 1564 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2078 2940 1394 Size: 429 255 862 3507 Fragments arranged by size: 3507 862 429 255 BsrDI GCAATG_nn' Cuts at: 65 1248 3548 3722 65 Size: 1183 2300 174 1396 Fragments arranged by size: 2300 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3697 3884 1700 Size: 110 1887 187 2869 Fragments arranged by size: 2869 1887 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5053 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5053 BssSI C'ACGA_G Cuts at: 3113 4125 3113 Size: 1012 4041 BstAPI GCAn_nnn'nTGC Cuts at: 794 2166 794 Size: 1372 3681 BstBI TT'CG_AA Cuts at: 3857 3857 Size: 5053 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5053 BstYI r'GATC_y Cuts at: 1972 2383 2750 3135 3152 4604 4690 4702 5048 Size: 411 367 385 17 1452 86 12 346 Cuts at: 5048 1972 Size: 1977 Fragments arranged by size: 1977 1452 411 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3562 Fragments arranged by size: 3562 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4314 Fragments arranged by size: 4314 683 56 BtsI GCAGTG_nn' Cuts at: 2323 2569 3363 3383 2323 Size: 246 794 20 3993 Fragments arranged by size: 3993 794 246 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5053 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5018 DraI TTT'AAA Cuts at: 2357 2357 Size: 5053 DraIII CAC_nnn'GTG Cuts at: 2319 2319 Size: 5053 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4060 809 Size: 507 2744 1802 Fragments arranged by size: 2744 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2500 3389 5018 Size: 54 1425 50 275 18 670 889 1629 Cuts at: 5018 8 Size: 43 Fragments arranged by size: 1629 1425 889 670 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2500 1537 Size: 275 688 4090 Fragments arranged by size: 4090 688 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2132 2983 1353 Size: 779 851 3423 Fragments arranged by size: 3423 851 779 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3640 4014 4160 387 Size: 1064 138 2051 374 146 1280 Fragments arranged by size: 2051 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2138 3116 3713 4500 4768 1239 Size: 393 506 978 597 787 268 1524 Fragments arranged by size: 1524 978 787 597 506 393 268 EcoRI G'AATT_C Cuts at: 2362 2740 2362 Size: 378 4675 FspI TGC'GCA Cuts at: 3559 3559 Size: 5053 HaeII r_GCGC'y Cuts at: 1678 4200 1678 Size: 2522 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3694 3726 3768 3800 1373 Size: 32 135 32 2122 32 42 32 2626 Fragments arranged by size: 2626 2122 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2397 2643 669 Size: 988 281 459 246 3079 Fragments arranged by size: 3079 988 459 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5053 HpaI GTT'AAC Cuts at: 2643 2643 Size: 5053 KpnI G_GTAC'C Cuts at: 2378 2378 Size: 5053 MfeI C'AATT_G Cuts at: 2652 2652 Size: 5053 MluI A'CGCG_T Cuts at: 4929 4929 Size: 5053 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4167 4351 1305 Size: 2862 184 2007 Fragments arranged by size: 2862 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3231 Fragments arranged by size: 3231 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2285 3011 3370 3529 518 Size: 648 1119 726 359 159 2042 Fragments arranged by size: 2042 1119 726 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2469 3146 4294 4539 1606 Size: 863 677 1148 245 2120 Fragments arranged by size: 2120 1148 863 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3562 Fragments arranged by size: 3562 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5053 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5053 NotI GC'GGCC_GC Cuts at: 2500 2500 Size: 5053 NspI r_CATG'y Cuts at: 1132 1229 2066 2411 3956 1132 Size: 97 837 345 1545 2229 Fragments arranged by size: 2229 1545 837 345 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5053 PciI A'CATG_T Cuts at: 3952 3952 Size: 5053 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5053 PmeI GTTT'AAAC Cuts at: 2357 2357 Size: 5053 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2222 2254 3122 3154 4635 4667 2222 Size: 32 868 32 1481 32 2608 Fragments arranged by size: 2608 1481 868 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5053 PsiI TTA'TAA Cuts at: 2623 2623 Size: 5053 PstI C_TGCA'G Cuts at: 830 2330 2405 830 Size: 1500 75 3478 Fragments arranged by size: 3478 1500 75 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5053 SalI G'TCGA_C Cuts at: 1936 2395 1936 Size: 459 4594 SbfI CC_TGCA'GG Cuts at: 2405 2405 Size: 5053 ScaI AGT'ACT Cuts at: 2332 3301 5013 2332 Size: 969 1712 2372 Fragments arranged by size: 2372 1712 969 SfcI C'TryA_G Cuts at: 826 1045 2014 2111 2326 2401 3536 4217 4408 Size: 219 969 97 215 75 1135 681 191 Cuts at: 4408 826 Size: 1471 Fragments arranged by size: 1471 1135 969 681 219 215 191 97 75 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5053 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5053 SmaI CCC'GGG Cuts at: 1872 2380 1872 Size: 508 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3157 4058 4320 820 Size: 197 926 1214 901 262 1553 Fragments arranged by size: 1553 1214 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2844 493 Size: 2351 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5053 SphI G_CATG'C Cuts at: 1132 2411 1132 Size: 1279 3774 SspI AAT'ATT Cuts at: 5 52 2977 5 Size: 47 2925 2081 Fragments arranged by size: 2925 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5053 StyI C'CwwG_G Cuts at: 513 1065 2004 2492 513 Size: 552 939 488 3074 Fragments arranged by size: 3074 939 552 488 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3080 3097 3250 3435 3880 325 Size: 1255 1500 17 153 185 445 1498 Fragments arranged by size: 1500 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2330 3299 5011 Size: 274 80 33 51 1443 353 969 1712 Cuts at: 5011 96 Size: 138 Fragments arranged by size: 1712 1443 969 353 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5013 5045 5013 Size: 32 5021 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5053 XbaI T'CTAG_A Cuts at: 2177 2389 2177 Size: 212 4841 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5053 XmaI C'CCGG_G Cuts at: 1870 2378 1870 Size: 508 4545 XmnI GAAnn'nnTTC Cuts at: 3182 3182 Size: 5053 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2861 276 Size: 53 83 186 936 1327 2468 Fragments arranged by size: 2468 1327 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI