Restriction map for pFN21AE5140
 (Circular) (Six-base) MAPSORT of: pFN21AE5140.seq  Check: 8178  from: 1  to: 5053



pFN21AE5140  5053 bp

 With 182 enzymes: SgfI * 

                        January 30, 2014 17:13  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    2863     278 
   Size:         53      83     186     936    1327    2468

  Fragments arranged by size: 

                2468    1327     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2396    1937 
   Size:        459    4594
 
Acc65I G'GTAC_C

Cuts at:   2374    2374 
   Size:       5053
 
AclI AA'CG_TT

Cuts at:   1563    3180    3553    1563 
   Size:       1617     373    3063

  Fragments arranged by size: 

                3063    1617     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2138    3116    4500    4768    1632 
   Size:        506     978    1384     268    1917

  Fragments arranged by size: 

                1917    1384     978     506     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    4856
 
AflIII A'CryG_T

Cuts at:   1203    3952    4929    1203 
   Size:       2749     977    1327

  Fragments arranged by size: 

                2749    1327     977
 
AgeI A'CCGG_T

Cuts at:   3884    3884 
   Size:       5053
 
AhdI GACnn_n'nnGTC

Cuts at:   3782    3782 
   Size:       5053
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5053
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5053
 
AlwNI CAG_nnn'CTG

Cuts at:   2796    4368    2796 
   Size:       1572    3481
 
ApaLI G'TGCA_C

Cuts at:   3110    4266    3110 
   Size:       1156    3897
 
ApoI r'AATT_y

Cuts at:   1493    2362    2588    2740    4849    1493 
   Size:        869     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     869     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3607     160 
   Size:        872    2575    1606

  Fragments arranged by size: 

                2575    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5053
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2378    1870 
   Size:         73     435    4545

  Fragments arranged by size: 

                4545     435      73
 
BamHI G'GATC_C

Cuts at:   2383    2750    2383 
   Size:        367    4686
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2141    2374    3829     618 
   Size:        325     217     627     354     233    1455    1842

  Fragments arranged by size: 

                1842    1455     627     354     325     233     217
 
BanII G_rGCy'C

Cuts at:   1391    2372    1391 
   Size:        981    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2216     928 
   Size:        971     317    3765

  Fragments arranged by size: 

                3765     971     317
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3244    3278    1903 
   Size:         34    1307      34    3678

  Fragments arranged by size: 

                3678    1307      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2945    4155    2945 
   Size:       1210    3843
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5053
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    3664     136 
   Size:        107     122      71     631     605    1992    1525

  Fragments arranged by size: 

                1992    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5048    5048 
   Size:       5053
 
BlpI GC'TnA_GC

Cuts at:   2470    2470 
   Size:       5053
 
Bme1580I G_kGCm'C

Cuts at:   1790    2146    3114    4270    1790 
   Size:        356     968    1156    2573

  Fragments arranged by size: 

                2573    1156     968     356
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5053
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2784    3742     442 
   Size:        416      53      83     399      24    1367     958    1753

  Fragments arranged by size: 

                1753    1367     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5053
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    3713    1239 
   Size:       2474    2579
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3178    4043    4341    3178 
   Size:        865     298    3890

  Fragments arranged by size: 

                3890     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    3716     882 
   Size:        617    2217    2219

  Fragments arranged by size: 

                2219    2217     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2844     493 
   Size:        711    1640    2702

  Fragments arranged by size: 

                2702    1640     711
 
BsaBI GATnn'nnATC

Cuts at:   5047    5047 
   Size:       5053
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2860    3242    3877     275 
   Size:         53      83     186     936    1327     382     635    1451

  Fragments arranged by size: 

                1451    1327     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3486    3884    4158    4305    4716    3486 
   Size:        398     274     147     411    3823

  Fragments arranged by size: 

                3823     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    4828

  Fragments arranged by size: 

                4828     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5053
 
BseYI C'CCAG_C

Cuts at:   2133    4256    4752    2133 
   Size:       2123     496    2434

  Fragments arranged by size: 

                2434    2123     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2108    1163 
   Size:        573     372    4108

  Fragments arranged by size: 

                4108     573     372
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2503    3264    4292    1540 
   Size:        275     186     502     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     502     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3114    3199    4270    3114 
   Size:         85    1071    3897

  Fragments arranged by size: 

                3897    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2108    2564    2657    2108 
   Size:        456      93    4504

  Fragments arranged by size: 

                4504     456      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2146    2372    3114    3199    4270    1391 
   Size:        399     356     226     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     399     356     226      85
 
BspEI T'CCGG_A

Cuts at:   4716    4716 
   Size:       5053
 
BspHI T'CATG_A

Cuts at:   2942    4672    2942 
   Size:       1730    3323
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2408     844 
   Size:       1564    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2078    2940    1394 
   Size:        429     255     862    3507

  Fragments arranged by size: 

                3507     862     429     255
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3548    3722      65 
   Size:       1183    2300     174    1396

  Fragments arranged by size: 

                2300    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3697    3884    1700 
   Size:        110    1887     187    2869

  Fragments arranged by size: 

                2869    1887     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5053
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5053
 
BssSI C'ACGA_G

Cuts at:   3113    4125    3113 
   Size:       1012    4041
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2166     794 
   Size:       1372    3681
 
BstBI TT'CG_AA

Cuts at:   3857    3857 
   Size:       5053
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5053
 
BstYI r'GATC_y

Cuts at:   1972    2383    2750    3135    3152    4604    4690    4702    5048 
   Size:        411     367     385      17    1452      86      12     346

Cuts at:   5048    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452     411     385     367     346      86      17
                  12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3562

  Fragments arranged by size: 

                3562     939     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4314

  Fragments arranged by size: 

                4314     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2323    2569    3363    3383    2323 
   Size:        246     794      20    3993

  Fragments arranged by size: 

                3993     794     246      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5053
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5018
 
DraI TTT'AAA

Cuts at:   2357    2357 
   Size:       5053
 
DraIII CAC_nnn'GTG

Cuts at:   2319    2319 
   Size:       5053
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4060     809 
   Size:        507    2744    1802

  Fragments arranged by size: 

                2744    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2500    3389    5018 
   Size:         54    1425      50     275      18     670     889    1629

Cuts at:   5018       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425     889     670     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2500    1537 
   Size:        275     688    4090

  Fragments arranged by size: 

                4090     688     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2132    2983    1353 
   Size:        779     851    3423

  Fragments arranged by size: 

                3423     851     779
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3640    4014    4160     387 
   Size:       1064     138    2051     374     146    1280

  Fragments arranged by size: 

                2051    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2138    3116    3713    4500    4768    1239 
   Size:        393     506     978     597     787     268    1524

  Fragments arranged by size: 

                1524     978     787     597     506     393     268
 
EcoRI G'AATT_C

Cuts at:   2362    2740    2362 
   Size:        378    4675
 
FspI TGC'GCA

Cuts at:   3559    3559 
   Size:       5053
 
HaeII r_GCGC'y

Cuts at:   1678    4200    1678 
   Size:       2522    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    3694    3726    3768    3800    1373 
   Size:         32     135      32    2122      32      42      32    2626

  Fragments arranged by size: 

                2626    2122     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2397    2643     669 
   Size:        988     281     459     246    3079

  Fragments arranged by size: 

                3079     988     459     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5053
 
HpaI GTT'AAC

Cuts at:   2643    2643 
   Size:       5053
 
KpnI G_GTAC'C

Cuts at:   2378    2378 
   Size:       5053
 
MfeI C'AATT_G

Cuts at:   2652    2652 
   Size:       5053
 
MluI A'CGCG_T

Cuts at:   4929    4929 
   Size:       5053
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4167    4351    1305 
   Size:       2862     184    2007

  Fragments arranged by size: 

                2862    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3231

  Fragments arranged by size: 

                3231    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2285    3011    3370    3529     518 
   Size:        648    1119     726     359     159    2042

  Fragments arranged by size: 

                2042    1119     726     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2469    3146    4294    4539    1606 
   Size:        863     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     863     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3562

  Fragments arranged by size: 

                3562     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5053
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5053
 
NotI GC'GGCC_GC

Cuts at:   2500    2500 
   Size:       5053
 
NspI r_CATG'y

Cuts at:   1132    1229    2066    2411    3956    1132 
   Size:         97     837     345    1545    2229

  Fragments arranged by size: 

                2229    1545     837     345      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5053
 
PciI A'CATG_T

Cuts at:   3952    3952 
   Size:       5053
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5053
 
PmeI GTTT'AAAC

Cuts at:   2357    2357 
   Size:       5053
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2222    2254    3122    3154    4635    4667    2222 
   Size:         32     868      32    1481      32    2608

  Fragments arranged by size: 

                2608    1481     868      32      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5053
 
PsiI TTA'TAA

Cuts at:   2623    2623 
   Size:       5053
 
PstI C_TGCA'G

Cuts at:    830    2330    2405     830 
   Size:       1500      75    3478

  Fragments arranged by size: 

                3478    1500      75
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5053
 
SalI G'TCGA_C

Cuts at:   1936    2395    1936 
   Size:        459    4594
 
SbfI CC_TGCA'GG

Cuts at:   2405    2405 
   Size:       5053
 
ScaI AGT'ACT

Cuts at:   2332    3301    5013    2332 
   Size:        969    1712    2372

  Fragments arranged by size: 

                2372    1712     969
 
SfcI C'TryA_G

Cuts at:    826    1045    2014    2111    2326    2401    3536    4217    4408 
   Size:        219     969      97     215      75    1135     681     191

Cuts at:   4408     826 
   Size:       1471

  Fragments arranged by size: 

                1471    1135     969     681     219     215     191      97
                  75
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5053
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5053
 
SmaI CCC'GGG

Cuts at:   1872    2380    1872 
   Size:        508    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3157    4058    4320     820 
   Size:        197     926    1214     901     262    1553

  Fragments arranged by size: 

                1553    1214     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    2844     493 
   Size:       2351    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5053
 
SphI G_CATG'C

Cuts at:   1132    2411    1132 
   Size:       1279    3774
 
SspI AAT'ATT

Cuts at:      5      52    2977       5 
   Size:         47    2925    2081

  Fragments arranged by size: 

                2925    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5053
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2492     513 
   Size:        552     939     488    3074

  Fragments arranged by size: 

                3074     939     552     488
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3080    3097    3250    3435    3880     325 
   Size:       1255    1500      17     153     185     445    1498

  Fragments arranged by size: 

                1500    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2330    3299    5011 
   Size:        274      80      33      51    1443     353     969    1712

Cuts at:   5011      96 
   Size:        138

  Fragments arranged by size: 

                1712    1443     969     353     274     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5013    5045    5013 
   Size:         32    5021
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5053
 
XbaI T'CTAG_A

Cuts at:   2177    2389    2177 
   Size:        212    4841
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5053
 
XmaI C'CCGG_G

Cuts at:   1870    2378    1870 
   Size:        508    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3182    3182 
   Size:       5053
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    2861     276 
   Size:         53      83     186     936    1327    2468

  Fragments arranged by size: 

                2468    1327     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI  Eco57MI
    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NcoI     NdeI
     NheI     NotI     NspI     PasI     PciI    PflMI     PmeI     PpiI
    PshAI     PsiI     PstI     PvuI     SalI     SbfI     ScaI     SfcI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I
   Bsu36I  EcoICRI    EcoNI EcoO109I    EcoRV     FalI     FseI    FspAI
     KasI     NaeI     NarI   NgoMIV     NruI     NsiI     PacI     PfoI
     PmlI    PpuMI   PspOMI    PspXI     PsrI    PvuII    RsrII     SacI
    SacII    SanDI     SapI    SexAI     SfiI     SfoI     SrfI     SwaI
     XcmI
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