Restriction map for
pFN21AE2373
(Linear) (Six-base) MAPSORT of: KIEE2373.seq Check: 4021 from: 1 to: 3429
KIEE2373.orf
With 182 enzymes: SgfI *
September 6, 2011 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1950 2533 3274 3429
Size: 1950 583 741 155
Fragments arranged by size:
1950 741 583 155
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 544 594 2419 3429
Size: 544 50 1825 1010
Fragments arranged by size:
1825 1010 544 50
AfeI AGC'GCT
Cuts at: 0 275 1219 3429
Size: 275 944 2210
Fragments arranged by size:
2210 944 275
AflIII A'CryG_T
Cuts at: 0 103 733 3026 3429
Size: 103 630 2293 403
Fragments arranged by size:
2293 630 403 103
AleI CACnn'nnGTG
Cuts at: 0 580 1018 3400 3429
Size: 580 438 2382 29
Fragments arranged by size:
2382 580 438 29
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 388 2230 2369 3429
Size: 388 1842 139 1060
Fragments arranged by size:
1842 1060 388 139
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2299 2331 3429
Size: 2299 32 1098
Fragments arranged by size:
2299 1098 32
AlwNI CAG_nnn'CTG
Cuts at: 0 360 522 594 891 2373 2568 2582 2895
Size: 360 162 72 297 1482 195 14 313
Cuts at: 2895 3130 3182 3301 3429
Size: 235 52 119 128
Fragments arranged by size:
1482 360 313 297 235 195 162 128
119 72 52 14
ApaI G_GGCC'C
Cuts at: 0 1489 1698 2100 2160 3293 3429
Size: 1489 209 402 60 1133 136
Fragments arranged by size:
1489 1133 402 209 136 60
ApaLI G'TGCA_C
Cuts at: 0 624 3429
Size: 624 2805
ApoI r'AATT_y
Cuts at: 0 1556 3429
Size: 1556 1873
AvaI C'yCGr_G
Cuts at: 0 2545 3429
Size: 2545 884
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2906 2939 3429
Size: 2906 33 490
Fragments arranged by size:
2906 490 33
BamHI G'GATC_C
Cuts at: 0 1352 3429
Size: 1352 2077
BanI G'GyrC_C
Cuts at: 0 582 990 1284 1997 2051 2506 3429
Size: 582 408 294 713 54 455 923
Fragments arranged by size:
923 713 582 455 408 294 54
BanII G_rGCy'C
Cuts at: 0 54 352 408 1489 1643 1698 1789 2100
Size: 54 298 56 1081 154 55 91 311
Cuts at: 2100 2160 3293 3429
Size: 60 1133 136
Fragments arranged by size:
1133 1081 311 298 154 136 91 60
56 55 54
BbeI G_GCGC'C
Cuts at: 0 2055 2510 3429
Size: 2055 455 919
Fragments arranged by size:
2055 919 455
BbsI GAAGACnn'nnnn_
Cuts at: 0 1140 1935 2523 3337 3429
Size: 1140 795 588 814 92
Fragments arranged by size:
1140 814 795 588 92
BbvCI CC'TCA_GC
Cuts at: 0 1153 2635 3302 3429
Size: 1153 1482 667 127
Fragments arranged by size:
1482 1153 667 127
BciVI GTATCCnnnnn_n'
Cuts at: 0 2907 3429
Size: 2907 522
BfrBI ATG'CAT
Cuts at: 0 1307 3429
Size: 1307 2122
BglI GCCn_nnn'nGGC
Cuts at: 0 903 999 1475 1484 2009 3080 3429
Size: 903 96 476 9 525 1071 349
Fragments arranged by size:
1071 903 525 476 349 96 9
BlpI GC'TnA_GC
Cuts at: 0 2361 3429
Size: 2361 1068
Bme1580I G_kGCm'C
Cuts at: 0 587 628 1489 1698 2100 2160 2844 2859
Size: 587 41 861 209 402 60 684 15
Cuts at: 2859 3293 3429
Size: 434 136
Fragments arranged by size:
861 684 587 434 402 209 136 60
41 15
BmgBI CAC'GTC
Cuts at: 0 1052 3429
Size: 1052 2377
BmrI ACTGGGnnnn_n'
Cuts at: 0 370 489 582 807 3429
Size: 370 119 93 225 2622
Fragments arranged by size:
2622 370 225 119 93
BmtI G_CTAG'C
Cuts at: 0 1741 2791 3429
Size: 1741 1050 638
Fragments arranged by size:
1741 1050 638
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 210 868 942 1117 1204 1313 1924 2229
Size: 210 658 74 175 87 109 611 305
Cuts at: 2229 2590 2674 2686 2797 2917 3262 3310 3429
Size: 361 84 12 111 120 345 48 119
Fragments arranged by size:
658 611 361 345 305 210 175 120
119 111 109 87 84 74 48 12
Bpu10I CC'TnA_GC
Cuts at: 0 1153 2374 2635 2947 3302 3429
Size: 1153 1221 261 312 355 127
Fragments arranged by size:
1221 1153 355 312 261 127
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 898 3429
Size: 202 696 2531
Fragments arranged by size:
2531 696 202
BsaI GGTCTCn'nnnn_
Cuts at: 0 32 1324 2650 3429
Size: 32 1292 1326 779
Fragments arranged by size:
1326 1292 779 32
BsaAI yAC'GTr
Cuts at: 0 3426 3429
Size: 3426 3
BsaBI GATnn'nnATC
Cuts at: 0 1777 3429
Size: 1777 1652
BsaHI Gr'CG_yC
Cuts at: 0 450 878 2052 2447 2507 3429
Size: 450 428 1174 395 60 922
Fragments arranged by size:
1174 922 450 428 395 60
BsaWI w'CCGG_w
Cuts at: 0 1298 1372 3413 3429
Size: 1298 74 2041 16
Fragments arranged by size:
2041 1298 74 16
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2227 2257 2615 2645 3429
Size: 2227 30 358 30 784
Fragments arranged by size:
2227 784 358 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 250 1132 1141 2032 2200 2242 2623 2914
Size: 250 882 9 891 168 42 381 291
Cuts at: 2914 2917 2920 2923 3429
Size: 3 3 3 506
Fragments arranged by size:
891 882 506 381 291 250 168 42
9 3 3 3
BseYI C'CCAG_C
Cuts at: 0 55 270 629 802 1214 1220 1742 2092
Size: 55 215 359 173 412 6 522 350
Cuts at: 2092 2268 2409 2846 2885 3051 3429
Size: 176 141 437 39 166 378
Fragments arranged by size:
522 437 412 378 359 350 215 176
173 166 141 55 39 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1222 1978 2146 2500 2539 3429
Size: 1222 756 168 354 39 890
Fragments arranged by size:
1222 890 756 354 168 39
BsiEI CG_ry'CG
Cuts at: 0 1900 3429
Size: 1900 1529
BsiHKAI G_wGCw'C
Cuts at: 0 533 628 733 1817 3429
Size: 533 95 105 1084 1612
Fragments arranged by size:
1612 1084 533 105 95
BsmBI CGTCTCn'nnnn_
Cuts at: 0 94 2591 3429
Size: 94 2497 838
Fragments arranged by size:
2497 838 94
Bsp1286I G_dGCh'C
Cuts at: 0 54 352 408 533 587 628 733 1489
Size: 54 298 56 125 54 41 105 756
Cuts at: 1489 1643 1698 1789 1817 2100 2160 2844 2859
Size: 154 55 91 28 283 60 684 15
Cuts at: 2859 3293 3429
Size: 434 136
Fragments arranged by size:
756 684 434 298 283 154 136 125
105 91 60 56 55 54 54 41
28 15
BspEI T'CCGG_A
Cuts at: 0 1298 1372 3429
Size: 1298 74 2057
Fragments arranged by size:
2057 1298 74
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 602 1950 2100 2533 3211 3274 3429
Size: 602 1348 150 433 678 63 155
Fragments arranged by size:
1348 678 602 433 155 150 63
BsrBI CCG'CTC
Cuts at: 0 474 849 1131 3429
Size: 474 375 282 2298
Fragments arranged by size:
2298 474 375 282
BsrDI GCAATG_nn'
Cuts at: 0 978 1322 3429
Size: 978 344 2107
Fragments arranged by size:
2107 978 344
BsrFI r'CCGG_y
Cuts at: 0 550 559 2723 2864 3429
Size: 550 9 2164 141 565
Fragments arranged by size:
2164 565 550 141 9
BsrGI T'GTAC_A
Cuts at: 0 3173 3429
Size: 3173 256
BssSI C'ACGA_G
Cuts at: 0 2182 3429
Size: 2182 1247
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1422 1997 3429
Size: 1422 575 1432
Fragments arranged by size:
1432 1422 575
BstEII G'GTnAC_C
Cuts at: 0 1135 1918 3429
Size: 1135 783 1511
Fragments arranged by size:
1511 1135 783
BstXI CCAn_nnnn'nTGG
Cuts at: 0 669 2937 3070 3429
Size: 669 2268 133 359
Fragments arranged by size:
2268 669 359 133
BstYI r'GATC_y
Cuts at: 0 716 1352 3429
Size: 716 636 2077
Fragments arranged by size:
2077 716 636
Bsu36I CC'TnA_GG
Cuts at: 0 1087 1124 3429
Size: 1087 37 2305
Fragments arranged by size:
2305 1087 37
BtgI C'CryG_G
Cuts at: 0 377 1075 2079 3429
Size: 377 698 1004 1350
Fragments arranged by size:
1350 1004 698 377
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 474 1806 3234 3429
Size: 474 1332 1428 195
Fragments arranged by size:
1428 1332 474 195
BtsI GCAGTG_nn'
Cuts at: 0 1234 3357 3429
Size: 1234 2123 72
Fragments arranged by size:
2123 1234 72
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 735 770 1305 1340 3429
Size: 735 35 535 35 2089
Fragments arranged by size:
2089 735 535 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 537 3429
Size: 537 2892
DrdI GACnn_nn'nnGTC
Cuts at: 0 916 1144 3429
Size: 916 228 2285
Fragments arranged by size:
2285 916 228
EaeI y'GGCC_r
Cuts at: 0 278 429 455 548 659 743 932 1256
Size: 278 151 26 93 111 84 189 324
Cuts at: 1256 1897 2039 2435 2762 2939 3429
Size: 641 142 396 327 177 490
Fragments arranged by size:
641 490 396 327 324 278 189 177
151 142 111 93 84 26
EagI C'GGCC_G
Cuts at: 0 1897 3429
Size: 1897 1532
EarI CTCTTCn'nnn_
Cuts at: 0 59 131 958 1591 1939 3429
Size: 59 72 827 633 348 1490
Fragments arranged by size:
1490 827 633 348 72 59
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 388 654 1054 1180 1492 3429
Size: 388 266 400 126 312 1937
Fragments arranged by size:
1937 400 388 312 266 126
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 210 544 594 868 942 1117 1204 1313
Size: 210 334 50 274 74 175 87 109
Cuts at: 1313 1924 2229 2419 2590 2674 2686 2797 2917
Size: 611 305 190 171 84 12 111 120
Cuts at: 2917 3262 3310 3429
Size: 345 48 119
Fragments arranged by size:
611 345 334 305 274 210 190 175
171 120 119 111 109 87 84 74
50 48 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2950 3429
Size: 2950 479
EcoO109I rG'GnC_Cy
Cuts at: 0 822 1486 1614 1694 1695 1713 1730 2096
Size: 822 664 128 80 1 18 17 366
Cuts at: 2096 2157 2370 2418 3037 3276 3290 3350 3429
Size: 61 213 48 619 239 14 60 79
Fragments arranged by size:
822 664 619 366 239 213 128 80
79 61 60 48 18 17 14 1
HaeII r_GCGC'y
Cuts at: 0 277 891 1178 1221 1247 1865 2055 2145
Size: 277 614 287 43 26 618 190 90
Cuts at: 2145 2154 2510 3429
Size: 9 356 919
Fragments arranged by size:
919 618 614 356 287 277 190 90
43 26 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 672 704 1383 1415 2227 2259 3429
Size: 672 32 679 32 812 32 1170
Fragments arranged by size:
1170 812 679 672 32 32 32
HindIII A'AGCT_T
Cuts at: 0 2320 3429
Size: 2320 1109
KasI G'GCGC_C
Cuts at: 0 2051 2506 3429
Size: 2051 455 923
Fragments arranged by size:
2051 923 455
MluI A'CGCG_T
Cuts at: 0 103 3429
Size: 103 3326
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2051 2835 3429
Size: 2051 784 594
Fragments arranged by size:
2051 784 594
MscI TGG'CCA
Cuts at: 0 431 457 661 2041 2941 3429
Size: 431 26 204 1380 900 488
Fragments arranged by size:
1380 900 488 431 204 26
MslI CAynn'nnrTG
Cuts at: 0 580 925 1018 1606 3031 3400 3429
Size: 580 345 93 588 1425 369 29
Fragments arranged by size:
1425 588 580 369 345 93 29
MspA1I CmG'CkG
Cuts at: 0 379 795 1077 1157 1461 1746 1902 2169
Size: 379 416 282 80 304 285 156 267
Cuts at: 2169 2343 2353 2388 2433 2846 2871 2895 3429
Size: 174 10 35 45 413 25 24 534
Fragments arranged by size:
534 416 413 379 304 285 282 267
174 156 80 45 35 25 24 10
NarI GG'CG_CC
Cuts at: 0 2052 2507 3429
Size: 2052 455 922
Fragments arranged by size:
2052 922 455
NheI G'CTAG_C
Cuts at: 0 1737 2787 3429
Size: 1737 1050 642
Fragments arranged by size:
1737 1050 642
NotI GC'GGCC_GC
Cuts at: 0 1897 3429
Size: 1897 1532
NsiI A_TGCA'T
Cuts at: 0 1309 3429
Size: 1309 2120
NspI r_CATG'y
Cuts at: 0 3030 3226 3429
Size: 3030 196 203
Fragments arranged by size:
3030 203 196
PasI CC'CwG_GG
Cuts at: 0 2278 3295 3429
Size: 2278 1017 134
Fragments arranged by size:
2278 1017 134
PciI A'CATG_T
Cuts at: 0 3026 3429
Size: 3026 403
PflMI CCAn_nnn'nTGG
Cuts at: 0 540 3286 3429
Size: 540 2746 143
Fragments arranged by size:
2746 540 143
PfoI T'CCnGG_A
Cuts at: 0 226 1550 1682 2303 3429
Size: 226 1324 132 621 1126
Fragments arranged by size:
1324 1126 621 226 132
PmlI CAC'GTG
Cuts at: 0 3426 3429
Size: 3426 3
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 720 752 3429
Size: 720 32 2677
Fragments arranged by size:
2677 720 32
PpuMI rG'GwC_Cy
Cuts at: 0 822 1614 1730 2370 3037 3429
Size: 822 792 116 640 667 392
Fragments arranged by size:
822 792 667 640 392 116
PshAI GACnn'nnGTC
Cuts at: 0 1336 3337 3429
Size: 1336 2001 92
Fragments arranged by size:
2001 1336 92
PspOMI G'GGCC_C
Cuts at: 0 1485 1694 2096 2156 3289 3429
Size: 1485 209 402 60 1133 140
Fragments arranged by size:
1485 1133 402 209 140 60
PstI C_TGCA'G
Cuts at: 0 203 467 518 1427 1592 2225 2348 2786
Size: 203 264 51 909 165 633 123 438
Cuts at: 2786 3347 3429
Size: 561 82
Fragments arranged by size:
909 633 561 438 264 203 165 123
82 51
PvuII CAG'CTG
Cuts at: 0 1746 2169 2343 2388 2433 2871 2895 3429
Size: 1746 423 174 45 45 438 24 534
Fragments arranged by size:
1746 534 438 423 174 45 45 24
SacII CC_GC'GG
Cuts at: 0 380 1078 3429
Size: 380 698 2351
Fragments arranged by size:
2351 698 380
SanDI GG'GwC_CC
Cuts at: 0 1614 1730 3037 3429
Size: 1614 116 1307 392
Fragments arranged by size:
1614 1307 392 116
SapI GCTCTTCn'nnn_
Cuts at: 0 958 1591 1939 3429
Size: 958 633 348 1490
Fragments arranged by size:
1490 958 633 348
SexAI A'CCwGG_T
Cuts at: 0 533 2672 3429
Size: 533 2139 757
Fragments arranged by size:
2139 757 533
SfcI C'TryA_G
Cuts at: 0 199 463 514 1423 1588 2124 2221 2344
Size: 199 264 51 909 165 536 97 123
Cuts at: 2344 2782 3343 3429
Size: 438 561 86
Fragments arranged by size:
909 561 536 438 264 199 165 123
97 86 51
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1475 1484 3429
Size: 1475 9 1945
Fragments arranged by size:
1945 1475 9
SfoI GGC'GCC
Cuts at: 0 2053 2508 3429
Size: 2053 455 921
Fragments arranged by size:
2053 921 455
SmlI C'TyrA_G
Cuts at: 0 217 913 3429
Size: 217 696 2516
Fragments arranged by size:
2516 696 217
SphI G_CATG'C
Cuts at: 0 3226 3429
Size: 3226 203
StuI AGG'CCT
Cuts at: 0 70 3367 3429
Size: 70 3297 62
Fragments arranged by size:
3297 70 62
StyI C'CwwG_G
Cuts at: 0 71 2930 3429
Size: 71 2859 499
Fragments arranged by size:
2859 499 71
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 643 1212 1228 1737 1944 3408 3429
Size: 643 569 16 509 207 1464 21
Fragments arranged by size:
1464 643 569 509 207 21 16
TatI w'GTAC_w
Cuts at: 0 3173 3429
Size: 3173 256
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1823 1855 3429
Size: 1823 32 1574
Fragments arranged by size:
1823 1574 32
Tth111I GACn'n_nGTC
Cuts at: 0 1050 3429
Size: 1050 2379
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1797 2292 2814 3429
Size: 1797 495 522 615
Fragments arranged by size:
1797 615 522 495
XmnI GAAnn'nnTTC
Cuts at: 0 3379 3429
Size: 3379 50
Enzymes that do cut:
AarI AcuI AfeI AflIII AleI AlfI AloI AlwNI
ApaI ApaLI ApoI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BfrBI BglI BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I HaeII Hin4I HindIII KasI
MluI MmeI MscI MslI MspA1I NarI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmlI PpiI
PpuMI PshAI PspOMI PstI PvuII SacII SanDI SapI
SexAI SfcI SfiI SfoI SmlI SphI StuI StyI
TaqII TatI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AscI
AseI AsiSI AvrII BcgI BclI BglII BplI BsiWI
BsmI BspHI BssHII BstBI BstZ17I ClaI DraI EcoICRI
EcoRI EcoRV FalI FseI FspI FspAI HincII HpaI
KpnI MfeI NaeI NcoI NdeI NgoMIV NruI PacI
PmeI PsiI PspXI PsrI PvuI RsrII SacI SalI
SbfI ScaI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI SwaI XbaI XhoI XmaI ZraI