Restriction map for
pFN21AB9896
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# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII AccIII AclI AflII AflIII
AgeI AhdI AscI AseI AsnI
Asp700I AspEI AspI AvrII BamHI
BbrPI BciVI BclI BfrI BlnI
BsaAI BsaBI BsaWI BseAI BsiWI
BsmI BspDI BspEI BspHI BspLU11I
BsrDI BssSI Bst1107I BstEII BstPI
BstZ17I ClaI CpoI DraI Eam1105I
EcoO65I EcoRV EcoT22I FbaI HincII
HindII HpaI MamI MfeI MluI
MroI MseI MunI NdeI NotI
NruI NsiI PacI PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
RcaI RsrII SalI SapI ScaI
SexAI SfiI SnaBI SpeI SspI
SwaI TfiI Tru9I Tth111I XbaI
XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AcsI 2584 GAATTC [GA]^AATT[CT]
Alw44I 1560 GTGCAC G^TGCAC
AocI 3150 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1088 AGCGCT AGC^GCT
ApaLI 1560 GTGCAC G^TGCAC
ApoI 2584 GAATTC [GA]^AATT[CT]
BbeI 3399 GGCGCC GGCGC^C
BbvCI 3427 GCTGAGG CCTCAGC(-5/-2)
BcgI 1181 GCAAGGCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 2075 CTAG C^TAG
BglII 2240 AGATCT A^GATCT
BlpI 2770 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2770 GCTGAGC GC^T[ACGT]AGC
Bsp1407I 2450 TGTACA T^GTACA
BsrGI 2450 TGTACA T^GTACA
BssHII 3436 GCGCGC G^CGCGC
BstBI 175 TTCGAA TT^CGAA
Bsu36I 3150 CCTGAGG CC^T[ACGT]AGG
CelII 2770 GCTGAGC GC^T[ACGT]AGC
DrdI 1468 GACAGCCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1510 CGGCCG C^GGCCG
EarI 460 GAAGAG CTCTTC(1/4)
EclXI 1510 CGGCCG C^GGCCG
Eco47III 1088 AGCGCT AGC^GCT
Eco52I 1510 CGGCCG C^GGCCG
Eco81I 3150 CCTGAGG CC^T[ACGT]AGG
EcoRI 2584 GAATTC G^AATTC
FseI 1927 GGCCGGCC GGCCGG^CC
HindIII 3451 AAGCTT A^AGCTT
KasI 3399 GGCGCC G^GCGCC
Ksp632I 460 GAAGAG CTCTTC(1/4)
KspI 3325 CCGCGG CCGC^GG
MaeI 2075 CTAG C^TAG
NaeI 1928 GCCGGC GCC^GGC
NarI 3399 GGCGCC GG^CGCC
NgoMIV 1928 GCCGGC G^CCGGC
NheI 2074 GCTAGC G^CTAGC
NspV 175 TTCGAA TT^CGAA
PaeR7I 3445 CTCGAG C^TCGAG
PvuI 3459 CGATCG CGAT^CG
SacII 3325 CCGCGG CCGC^GG
SbfI 2943 CCTGCAGG CCTGCA^GG
SfoI 3399 GGCGCC GGC^GCC
SfuI 175 TTCGAA TT^CGAA
SgrAI 2688 CACCGGCG C[GA]^CCGG[CT]G
Sse8387I 2943 CCTGCAGG CCTGCA^GG
SspBI 2450 TGTACA T^GTACA
Tsp509I 2585 AATT ^AATT
XcmI 1940 CCACCTCCCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 3445 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
Acc65I 2603 GGTACC G^GTACC
2939 GGTACC
AcyI 130 GACGCC G[GA]^CG[CT]C
3399 GGCGCC
Asp718I 2603 GGTACC G^GTACC
2939 GGTACC
BalI 2678 TGGCCA TGG^CCA
2705 TGGCCA
BpmI 1836 CTGGAG CTGGAG(16/14)
3319 CTGGAG
BsaHI 130 GACGCC G[GA]^CG[CT]C
3399 GGCGCC
DraIII 2342 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
2383 CACCCCGTG
Hin1I 130 GACGCC G[GA]^CG[CT]C
3399 GGCGCC
KpnI 2603 GGTACC GGTAC^C
2939 GGTACC
MaeII 1322 ACGT A^CGT
1673 ACGT
MluNI 2678 TGGCCA TGG^CCA
2705 TGGCCA
MscI 2678 TGGCCA TGG^CCA
2705 TGGCCA
PshAI 3262 GACCGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3513 GACCACTGTC
SacI 327 GAGCTC GAGCT^C
1123 GAGCTC
SphI 1532 GCATGC GCATG^C
3419 GCATGC
StuI 2330 AGGCCT AGG^CCT
3353 AGGCCT
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# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccI AccII AciI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII AviII BanI BanII BbsI
BbvI BcnI BglI BmyI BpuAI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
FspI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StyI TaqI TseI Tsp45I
TspRI TthHB8I Van91I XhoII XmaCI
XmaI