################################## # NO CUT ENZYMES ( 71 enzymes) # ################################## AatII Acc65I AclI AgeI AhdI Aor51HI ApaI AscI Asp718I AspEI AvrII BalI BamHI BbrPI BcgI BglI BlnI BsiEI BsmBI BsrFI BssHII Bst1107I BstAPI BstEII BstPI BstXI BstZ17I Cfr10I CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoT22I FseI HgaI KpnI MluI MluNI MscI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SacI SalI SbfI SfiI SgrAI SnaBI SphI Sse8387I XcmI XhoI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccIII 79 TCCGGA T^CCGGA AflII 4072 CTTAAG C^TTAAG Alw44I 4970 GTGCAC G^TGCAC ApaLI 4970 GTGCAC G^TGCAC AseI 2734 ATTAAT AT^TAAT AsnI 2734 ATTAAT AT^TAAT Asp700I 5631 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 4970 GTGCAC G[AT]GC[AT]^C BbvCI 6055 GCTGAGG CCTCAGC(-5/-2) BfrI 4072 CTTAAG C^TTAAG BglII 6062 AGATCT A^GATCT BseAI 79 TCCGGA T^CCGGA BsiHKAI 4970 GTGCAC G[AT]GC[AT]^C BsiWI 182 CGTACG C^GTACG BspDI 2258 ATCGAT AT^CGAT BspEI 79 TCCGGA T^CCGGA BspLU11I 2725 ACATGT A^CATGT BsrDI 2111 CATTGC GCAATG(2/0) BssSI 3608 CTCGTG CACGAG(-5/-1) ClaI 2258 ATCGAT AT^CGAT DraII 1850 AGGTCCT [GA]G^G[ACGT]CC[CT] EcoO109I 1850 AGGTCCT [GA]G^G[ACGT]CC[CT] EcoRV 973 GATATC GAT^ATC HincII 5068 GTTAAC GT[CT]^[GA]AC HindII 5068 GTTAAC GT[CT]^[GA]AC HpaI 5068 GTTAAC GTT^AAC KspI 233 CCGCGG CCGC^GG MfeI 261 CAATTG C^AATTG MroI 79 TCCGGA T^CCGGA MunI 261 CAATTG C^AATTG PpuMI 1850 AGGTCCT [GA]G^G[AT]CC[CT] PshAI 1518 GACTTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 2734 ATTAAT AT^TAAT PstI 5659 CTGCAG CTGCA^G SacII 233 CCGCGG CCGC^GG ScaI 1273 AGTACT AGT^ACT SmaI 296 CCCGGG CCC^GGG StuI 2277 AGGCCT AGG^CCT SwaI 2052 ATTTAAAT ATTT^AAAT XmaCI 296 CCCGGG C^CCGGG XmaI 296 CCCGGG C^CCGGG XmnI 5631 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 37 enzymes) # ################################### AccI 510 GTCTAC GT^[AC][GT]AC 5271 GTCTAC AccII 177 CGCG CG^CG 234 CGCG AcyI 117 GGCGCC G[GA]^CG[CT]C 238 GGCGCC AflIII 1267 ACACGT A^C[GA][CT]GT 2725 ACATGT AvaI 296 CCCGGG C^[CT]CG[GA]G 1430 CCCGAG AviII 5139 TGCGCA TGC^GCA 5433 TGCGCA BanI 117 GGCGCC G^G[CT][GA]CC 238 GGCGCC BbeI 117 GGCGCC GGCGC^C 238 GGCGCC BciVI 5217 GGATAC GTATCC(6/5) 476 GTATCC BlpI 142 GCTCAGC GC^T[ACGT]AGC 190 GCTGAGC Bpu1102I 142 GCTCAGC GC^T[ACGT]AGC 190 GCTGAGC BsaAI 1262 CACGTA [CT]AC^GT[GA] 1268 CACGTA BsaBI 471 GATGGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2975 GATTTTTATC BsaHI 117 GGCGCC G[GA]^CG[CT]C 238 GGCGCC BsaWI 79 TCCGGA [AT]^CCGG[AT] 337 ACCGGA BsoBI 296 CCCGGG C^[CT]CG[GA]G 1430 CCCGAG BsrBI 3 GAGCGG CCGCTC(-3/-3) 193 GAGCGG BstBI 2887 TTCGAA TT^CGAA 3825 TTCGAA BstUI 177 CGCG CG^CG 234 CGCG CelII 142 GCTCAGC GC^T[ACGT]AGC 190 GCTGAGC DraIII 1928 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG 3932 CACTGAGTG DsaI 233 CCGCGG C^C[GA][CT]GG 365 CCGTGG FspI 5139 TGCGCA TGC^GCA 5433 TGCGCA Hin1I 117 GGCGCC G[GA]^CG[CT]C 238 GGCGCC HindIII 518 AAGCTT A^AGCTT 862 AAGCTT KasI 117 GGCGCC G^GCGCC 238 GGCGCC MamI 471 GATGGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2975 GATTTTTATC MvnI 177 CGCG CG^CG 234 CGCG NarI 117 GGCGCC GG^CGCC 238 GGCGCC NspI 2725 ACATGT [GA]CATG^[CT] 4515 GCATGT NspV 2887 TTCGAA TT^CGAA 3825 TTCGAA PflMI 465 CCAGGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 915 CCAGATTTTGG SapI 1692 GAAGAGC GCTCTTC(1/4) 2893 GAAGAGC SexAI 464 ACCAGGT A^CC[AT]GGT 741 ACCAGGT SfoI 117 GGCGCC GGC^GCC 238 GGCGCC SfuI 2887 TTCGAA TT^CGAA 3825 TTCGAA Van91I 465 CCAGGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 915 CCAGATTTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 108 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI AocI ApoI AspI AvaII BanII BbsI BbvI BclI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I Bsp1407I BspHI BspMI BsrGI BsrI BssKI BstF5I BstNI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraI EaeI EarI Eco81I EcoRI EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PflFI PleI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SpeI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XbaI XhoII