(Linear) (Six-base) MAPSORT of: KIEE2314.seq Check: 2811 from: 1 to: 6144 KIEE2314.orf With 182 enzymes: SgfI * January 31, 2011 13:14 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 5666 6144 Size: 5666 478 AccI GT'mk_AC Cuts at: 0 511 5272 6144 Size: 511 4761 872 Fragments arranged by size: 4761 872 511 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 383 833 1262 1790 4577 5354 6144 Size: 383 450 429 528 2787 777 790 Fragments arranged by size: 2787 790 777 528 450 429 383 AflII C'TTAA_G Cuts at: 0 4072 6144 Size: 4072 2072 AflIII A'CryG_T Cuts at: 0 1267 2725 6144 Size: 1267 1458 3419 Fragments arranged by size: 3419 1458 1267 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 3124 6144 Size: 3124 3020 AlwNI CAG_nnn'CTG Cuts at: 0 3721 5658 5807 6144 Size: 3721 1937 149 337 Fragments arranged by size: 3721 1937 337 149 ApaLI G'TGCA_C Cuts at: 0 4970 6144 Size: 4970 1174 ApoI r'AATT_y Cuts at: 0 1324 2050 2180 2646 3054 3129 3141 3153 Size: 1324 726 130 466 408 75 12 12 Cuts at: 3153 3276 3324 3861 4131 4153 4416 5115 5208 Size: 123 48 537 270 22 263 699 93 Cuts at: 5208 5320 5792 6144 Size: 112 472 352 Fragments arranged by size: 1324 726 699 537 472 466 408 352 270 263 130 123 112 93 75 48 22 12 12 AseI AT'TA_AT Cuts at: 0 2735 6144 Size: 2735 3409 AvaI C'yCGr_G Cuts at: 0 296 1430 6144 Size: 296 1134 4714 Fragments arranged by size: 4714 1134 296 BanI G'GyrC_C Cuts at: 0 117 238 6144 Size: 117 121 5906 Fragments arranged by size: 5906 121 117 BanII G_rGCy'C Cuts at: 0 40 103 1699 6144 Size: 40 63 1596 4445 Fragments arranged by size: 4445 1596 63 40 BbeI G_GCGC'C Cuts at: 0 121 242 6144 Size: 121 121 5902 Fragments arranged by size: 5902 121 121 BbsI GAAGACnn'nnnn_ Cuts at: 0 405 1226 2681 4451 6144 Size: 405 821 1455 1770 1693 Fragments arranged by size: 1770 1693 1455 821 405 BbvCI CC'TCA_GC Cuts at: 0 6056 6144 Size: 6056 88 BciVI GTATCCnnnnn_n' Cuts at: 0 487 5211 6144 Size: 487 4724 933 Fragments arranged by size: 4724 933 487 BclI T'GATC_A Cuts at: 0 2247 3384 3612 6144 Size: 2247 1137 228 2532 Fragments arranged by size: 2532 2247 1137 228 BglII A'GATC_T Cuts at: 0 6062 6144 Size: 6062 82 BlpI GC'TnA_GC Cuts at: 0 143 191 6144 Size: 143 48 5953 Fragments arranged by size: 5953 143 48 Bme1580I G_kGCm'C Cuts at: 0 4974 6144 Size: 4974 1170 BmgBI CAC'GTC Cuts at: 0 32 4933 6144 Size: 32 4901 1211 Fragments arranged by size: 4901 1211 32 BmrI ACTGGGnnnn_n' Cuts at: 0 262 352 3364 6144 Size: 262 90 3012 2780 Fragments arranged by size: 3012 2780 262 90 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 169 806 1597 5902 6144 Size: 169 637 791 4305 242 Fragments arranged by size: 4305 791 637 242 169 Bpu10I CC'TnA_GC Cuts at: 0 3035 6056 6144 Size: 3035 3021 88 Fragments arranged by size: 3035 3021 88 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 24 2102 2147 3070 6144 Size: 24 2078 45 923 3074 Fragments arranged by size: 3074 2078 923 45 24 BsaI GGTCTCn'nnnn_ Cuts at: 0 1420 3522 5603 6144 Size: 1420 2102 2081 541 Fragments arranged by size: 2102 2081 1420 541 BsaAI yAC'GTr Cuts at: 0 1264 1270 6144 Size: 1264 6 4874 Fragments arranged by size: 4874 1264 6 BsaBI GATnn'nnATC Cuts at: 0 475 2979 6144 Size: 475 2504 3165 Fragments arranged by size: 3165 2504 475 BsaHI Gr'CG_yC Cuts at: 0 118 239 6144 Size: 118 121 5905 Fragments arranged by size: 5905 121 118 BsaWI w'CCGG_w Cuts at: 0 79 337 6144 Size: 79 258 5807 Fragments arranged by size: 5807 258 79 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 614 2981 4846 5038 5041 6073 6144 Size: 614 2367 1865 192 3 1032 71 Fragments arranged by size: 2367 1865 1032 614 192 71 3 BseYI C'CCAG_C Cuts at: 0 5407 6144 Size: 5407 737 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 210 271 564 3029 3095 3302 3959 4955 Size: 210 61 293 2465 66 207 657 996 Cuts at: 4955 6144 Size: 1189 Fragments arranged by size: 2465 1189 996 657 293 210 207 66 61 BsiHKAI G_wGCw'C Cuts at: 0 4974 6144 Size: 4974 1170 BsiWI C'GTAC_G Cuts at: 0 182 6144 Size: 182 5962 BsmI GAATG_Cn' Cuts at: 0 1398 2326 4473 6144 Size: 1398 928 2147 1671 Fragments arranged by size: 2147 1671 1398 928 Bsp1286I G_dGCh'C Cuts at: 0 40 103 1699 4974 6144 Size: 40 63 1596 3275 1170 Fragments arranged by size: 3275 1596 1170 63 40 BspEI T'CCGG_A Cuts at: 0 79 6144 Size: 79 6065 BspHI T'CATG_A Cuts at: 0 843 1226 3345 3648 5472 6144 Size: 843 383 2119 303 1824 672 Fragments arranged by size: 2119 1824 843 672 383 303 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 791 1839 5666 6144 Size: 791 1048 3827 478 Fragments arranged by size: 3827 1048 791 478 BsrBI CCG'CTC Cuts at: 0 5 195 6144 Size: 5 190 5949 Fragments arranged by size: 5949 190 5 BsrDI GCAATG_nn' Cuts at: 0 2110 6144 Size: 2110 4034 BsrGI T'GTAC_A Cuts at: 0 374 1710 3252 3624 3921 6144 Size: 374 1336 1542 372 297 2223 Fragments arranged by size: 2223 1542 1336 374 372 297 BssSI C'ACGA_G Cuts at: 0 3608 6144 Size: 3608 2536 BstBI TT'CG_AA Cuts at: 0 2888 3826 6144 Size: 2888 938 2318 Fragments arranged by size: 2888 2318 938 BstYI r'GATC_y Cuts at: 0 5131 5648 6062 6144 Size: 5131 517 414 82 Fragments arranged by size: 5131 517 414 82 Bsu36I CC'TnA_GG Cuts at: 0 572 1536 4313 6144 Size: 572 964 2777 1831 Fragments arranged by size: 2777 1831 964 572 BtgI C'CryG_G Cuts at: 0 233 365 6144 Size: 233 132 5779 Fragments arranged by size: 5779 233 132 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 5186 6144 Size: 5186 958 BtsI GCAGTG_nn' Cuts at: 0 4185 6144 Size: 4185 1959 ClaI AT'CG_AT Cuts at: 0 2259 6144 Size: 2259 3885 DraI TTT'AAA Cuts at: 0 2055 2558 3603 4304 4637 5114 5213 6144 Size: 2055 503 1045 701 333 477 99 931 Fragments arranged by size: 2055 1045 931 701 503 477 333 99 DraIII CAC_nnn'GTG Cuts at: 0 1933 3937 6144 Size: 1933 2004 2207 Fragments arranged by size: 2207 2004 1933 EaeI y'GGCC_r Cuts at: 0 494 1209 1527 1731 6144 Size: 494 715 318 204 4413 Fragments arranged by size: 4413 715 494 318 204 EarI CTCTTCn'nnn_ Cuts at: 0 1452 1687 2306 2888 4470 4823 6144 Size: 1452 235 619 582 1582 353 1321 Fragments arranged by size: 1582 1452 1321 619 582 353 235 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 2984 6144 Size: 2984 3160 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 169 383 806 833 1262 1597 1790 4577 Size: 169 214 423 27 429 335 193 2787 Cuts at: 4577 5354 5902 6144 Size: 777 548 242 Fragments arranged by size: 2787 777 548 429 423 335 242 214 193 169 27 EcoO109I rG'GnC_Cy Cuts at: 0 1851 6144 Size: 1851 4293 EcoRI G'AATT_C Cuts at: 0 2180 3129 3153 4131 5792 6144 Size: 2180 949 24 978 1661 352 Fragments arranged by size: 2180 1661 978 949 352 24 EcoRV GAT'ATC Cuts at: 0 975 6144 Size: 975 5169 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 429 1684 2611 2914 3488 4567 6144 Size: 429 1255 927 303 574 1079 1577 Fragments arranged by size: 1577 1255 1079 927 574 429 303 FspI TGC'GCA Cuts at: 0 5141 5435 6144 Size: 5141 294 709 Fragments arranged by size: 5141 709 294 HaeII r_GCGC'y Cuts at: 0 121 242 5783 6144 Size: 121 121 5541 361 Fragments arranged by size: 5541 361 121 121 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 680 712 825 857 1424 1456 2356 2388 Size: 680 32 113 32 567 32 900 32 Cuts at: 2388 2842 2874 4488 4520 6144 Size: 454 32 1614 32 1624 Fragments arranged by size: 1624 1614 900 680 567 454 113 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 5070 6144 Size: 5070 1074 HindIII A'AGCT_T Cuts at: 0 518 862 6144 Size: 518 344 5282 Fragments arranged by size: 5282 518 344 HpaI GTT'AAC Cuts at: 0 5070 6144 Size: 5070 1074 KasI G'GCGC_C Cuts at: 0 117 238 6144 Size: 117 121 5906 Fragments arranged by size: 5906 121 117 MfeI C'AATT_G Cuts at: 0 261 6144 Size: 261 5883 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 134 1326 1832 3312 3530 5908 6144 Size: 134 1192 506 1480 218 2378 236 Fragments arranged by size: 2378 1480 1192 506 236 218 134 MslI CAynn'nnrTG Cuts at: 0 1109 3162 5584 6097 6144 Size: 1109 2053 2422 513 47 Fragments arranged by size: 2422 2053 1109 513 47 MspA1I CmG'CkG Cuts at: 0 136 147 235 285 389 613 3124 6144 Size: 136 11 88 50 104 224 2511 3020 Fragments arranged by size: 3020 2511 224 136 104 88 50 11 NarI GG'CG_CC Cuts at: 0 118 239 6144 Size: 118 121 5905 Fragments arranged by size: 5905 121 118 NspI r_CATG'y Cuts at: 0 2729 4519 6144 Size: 2729 1790 1625 Fragments arranged by size: 2729 1790 1625 PciI A'CATG_T Cuts at: 0 2725 6144 Size: 2725 3419 PflMI CCAn_nnn'nTGG Cuts at: 0 471 921 6144 Size: 471 450 5223 Fragments arranged by size: 5223 471 450 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1448 1480 6144 Size: 1448 32 4664 Fragments arranged by size: 4664 1448 32 PpuMI rG'GwC_Cy Cuts at: 0 1851 6144 Size: 1851 4293 PshAI GACnn'nnGTC Cuts at: 0 1522 6144 Size: 1522 4622 PsiI TTA'TAA Cuts at: 0 1672 1793 2452 2837 2930 6144 Size: 1672 121 659 385 93 3214 Fragments arranged by size: 3214 1672 659 385 121 93 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 4714 4746 6144 Size: 4714 32 1398 Fragments arranged by size: 4714 1398 32 PstI C_TGCA'G Cuts at: 0 5663 6144 Size: 5663 481 PvuII CAG'CTG Cuts at: 0 147 285 3124 6144 Size: 147 138 2839 3020 Fragments arranged by size: 3020 2839 147 138 SacII CC_GC'GG Cuts at: 0 236 6144 Size: 236 5908 SapI GCTCTTCn'nnn_ Cuts at: 0 1687 2888 6144 Size: 1687 1201 3256 Fragments arranged by size: 3256 1687 1201 ScaI AGT'ACT Cuts at: 0 1275 6144 Size: 1275 4869 SexAI A'CCwGG_T Cuts at: 0 464 741 6144 Size: 464 277 5403 Fragments arranged by size: 5403 464 277 SfcI C'TryA_G Cuts at: 0 1992 3708 5313 5659 6066 6144 Size: 1992 1716 1605 346 407 78 Fragments arranged by size: 1992 1716 1605 407 346 78 SfoI GGC'GCC Cuts at: 0 119 240 6144 Size: 119 121 5904 Fragments arranged by size: 5904 121 119 SmaI CCC'GGG Cuts at: 0 298 6144 Size: 298 5846 SmlI C'TyrA_G Cuts at: 0 39 2117 2162 3049 4072 6144 Size: 39 2078 45 887 1023 2072 Fragments arranged by size: 2078 2072 1023 887 45 39 SpeI A'CTAG_T Cuts at: 0 693 2800 3868 6144 Size: 693 2107 1068 2276 Fragments arranged by size: 2276 2107 1068 693 SspI AAT'ATT Cuts at: 0 1044 2664 3219 3734 5061 6144 Size: 1044 1620 555 515 1327 1083 Fragments arranged by size: 1620 1327 1083 1044 555 515 StuI AGG'CCT Cuts at: 0 2279 6144 Size: 2279 3865 StyI C'CwwG_G Cuts at: 0 113 434 5147 6144 Size: 113 321 4713 997 Fragments arranged by size: 4713 997 321 113 SwaI ATTT'AAAT Cuts at: 0 2055 6144 Size: 2055 4089 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1624 6144 Size: 1624 4520 TatI w'GTAC_w Cuts at: 0 374 1273 1710 2728 3252 3624 3921 4012 Size: 374 899 437 1018 524 372 297 91 Cuts at: 4012 4730 6144 Size: 718 1414 Fragments arranged by size: 1414 1018 899 718 524 437 374 372 297 91 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 526 558 6144 Size: 526 32 5586 Fragments arranged by size: 5586 526 32 Tth111I GACn'n_nGTC Cuts at: 0 507 1191 4502 6144 Size: 507 684 3311 1642 Fragments arranged by size: 3311 1642 684 507 XbaI T'CTAG_A Cuts at: 0 207 595 1245 3765 4161 6144 Size: 207 388 650 2520 396 1983 Fragments arranged by size: 2520 1983 650 396 388 207 XmaI C'CCGG_G Cuts at: 0 296 6144 Size: 296 5848 XmnI GAAnn'nnTTC Cuts at: 0 5635 6144 Size: 5635 509 Enzymes that do cut: AarI AccI AcuI AflII AflIII AlfI AlwNI ApaLI ApoI AseI AvaI BanI BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrGI BssSI BstBI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII EaeI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI MfeI MmeI MslI MspA1I NarI NspI PciI PflMI PpiI PpuMI PshAI PsiI PsrI PstI PvuII SacII SapI ScaI SexAI SfcI SfoI SmaI SmlI SpeI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XmaI XmnI Enzymes that do not cut: AatII Acc65I AclI AfeI AgeI AhdI AleI AloI ApaI AscI AsiSI AvrII BaeI BamHI BcgI BfrBI BglI BmtI BplI BsaXI BsiEI BsmBI BsrFI BssHII BstAPI BstEII BstXI BstZ17I CspCI DrdI EagI EcoICRI EcoNI FseI FspAI KpnI MluI MscI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PfoI PmeI PmlI PspOMI PspXI PvuI RsrII SacI SalI SanDI SbfI SfiI SgfI SgrAI SnaBI SphI SrfI XcmI XhoI ZraI