################################## # NO CUT ENZYMES ( 65 enzymes) # ################################## AatII Acc65I AflII AgeI Aor51HI AscI AseI AsnI Asp718I AspI AviII BbvCI BclI BfrI BglI BsiEI BsiWI BspDI BssHII BstBI ClaI CpoI DraI EagI EclXI Eco47III Eco52I EcoRI FbaI FseI FspI HpaI KpnI KspI MfeI MunI NaeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmeI PshBI PvuI RsrII SacII SalI SfiI SfuI SgrAI SmaI SnaBI SpeI SspI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AccII 5982 CGCG CG^CG AccIII 3395 TCCGGA T^CCGGA AhdI 5347 GACCACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApaI 4046 GGGCCC GGGCC^C AspEI 5347 GACCACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 5442 CCTAGG C^CTAGG BbeI 5430 GGCGCC GGCGC^C BbrPI 383 CACGTG CAC^GTG BciVI 4992 GGATAC GTATCC(6/5) BlnI 5442 CCTAGG C^CTAGG BsaBI 1486 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 3395 TCCGGA T^CCGGA BspEI 3395 TCCGGA T^CCGGA BspLU11I 2219 ACATGT A^CATGT BsrBI 4444 GAGCGG CCGCTC(-3/-3) BssSI 2386 CTCGTG CACGAG(-5/-1) BstEII 5844 GGTCACC G^GT[ACGT]ACC BstPI 5844 GGTCACC G^GT[ACGT]ACC BstUI 5982 CGCG CG^CG DraIII 1010 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 5347 GACCACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 5844 GGTCACC G^GT[ACGT]ACC EcoRV 388 GATATC GAT^ATC KasI 5430 GGCGCC G^GCGCC MamI 1486 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluI 5981 ACGCGT A^CGCGT MroI 3395 TCCGGA T^CCGGA MvnI 5982 CGCG CG^CG NarI 5430 GGCGCC GG^CGCC NdeI 2063 CATATG CA^TATG PmaCI 383 CACGTG CAC^GTG PmlI 383 CACGTG CAC^GTG PshAI 1867 GACAGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SfoI 5430 GGCGCC GGC^GCC StuI 5771 AGGCCT AGG^CCT ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AclI 3178 AACGTT AA^CGTT 5915 AACGTT AcyI 4886 GACGCC G[GA]^CG[CT]C 5430 GGCGCC Alw44I 1007 GTGCAC G^TGCAC 3562 GTGCAC ApaLI 1007 GTGCAC G^TGCAC 3562 GTGCAC Asp700I 3435 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 5130 GAAGGATTTC BamHI 1297 GGATCC G^GATCC 1448 GGATCC BglII 1167 AGATCT A^GATCT 2862 AGATCT BsaAI 383 CACGTG [CT]AC^GT[GA] 1994 TACGTG BsaHI 4886 GACGCC G[GA]^CG[CT]C 5430 GGCGCC BsaWI 86 ACCGGA [AT]^CCGG[AT] 3395 TCCGGA BsmBI 4691 CGTCTC CGTCTC(1/5) 1480 GAGACG Bsp1407I 2498 TGTACA T^GTACA 4130 TGTACA BsrFI 230 ACCGGC [GA]^CCGG[CT] 3266 GCCGGT BsrGI 2498 TGTACA T^GTACA 4130 TGTACA BstXI 1589 CCATCAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4490 CCAAGGATGTGG Cfr10I 230 ACCGGC [GA]^CCGG[CT] 3266 GCCGGT DrdI 694 GACGAGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1940 GACTCATTGGTC EcoT22I 791 ATGCAT ATGCA^T 5194 ATGCAT Hin1I 4886 GACGCC G[GA]^CG[CT]C 5430 GGCGCC HincII 1999 GTTGAC GT[CT]^[GA]AC 2374 GTTGAC HindII 1999 GTTGAC GT[CT]^[GA]AC 2374 GTTGAC HindIII 1886 AAGCTT A^AGCTT 2686 AAGCTT MseI 953 TTAA T^TAA 4007 TTAA NsiI 791 ATGCAT ATGCA^T 5194 ATGCAT Psp1406I 3178 AACGTT AA^CGTT 5915 AACGTT SacI 4110 GAGCTC GAGCT^C 5488 GAGCTC SbfI 5316 CCTGCAGG CCTGCA^GG 5682 CCTGCAGG SexAI 1238 ACCTGGT A^CC[AT]GGT 4976 ACCTGGT Sse8387I 5316 CCTGCAGG CCTGCA^GG 5682 CCTGCAGG SspBI 2498 TGTACA T^GTACA 4130 TGTACA Tru9I 953 TTAA T^TAA 4007 TTAA XmnI 3435 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 5130 GAAGGATTTC ######################################### # 3 OR MORE CUT ENZYMES ( 126 enzymes) # ######################################### AccI AciI AcsI AfaI AflIII AluI AlwI AlwNI AocI ApoI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcgI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspHI BspMI BsrDI BsrI BssKI Bst1107I BstAPI BstF5I BstNI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RcaI RsaI SapI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SphI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII