(Linear) (Six-base) MAPSORT of: KIEE2312.seq Check: 5562 from: 1 to: 6408 KIEE2312.orf With 182 enzymes: SgfI * January 11, 2011 13:49 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 3575 6408 Size: 3575 2833 AccI GT'mk_AC Cuts at: 0 647 4016 4166 6408 Size: 647 3369 150 2242 Fragments arranged by size: 3369 2242 647 150 AclI AA'CG_TT Cuts at: 0 3179 5916 6408 Size: 3179 2737 492 Fragments arranged by size: 3179 2737 492 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1369 2341 3034 3613 4267 4890 5038 5478 Size: 1369 972 693 579 654 623 148 440 Cuts at: 5478 5899 5974 6408 Size: 421 75 434 Fragments arranged by size: 1369 972 693 654 623 579 440 434 421 148 75 AflIII A'CryG_T Cuts at: 0 1995 2219 4034 5981 6408 Size: 1995 224 1815 1947 427 Fragments arranged by size: 1995 1947 1815 427 224 AhdI GACnn_n'nnGTC Cuts at: 0 5352 6408 Size: 5352 1056 AleI CACnn'nnGTG Cuts at: 0 2494 6408 Size: 2494 3914 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1358 4348 5729 6408 Size: 1358 2990 1381 679 Fragments arranged by size: 2990 1381 1358 679 AlwNI CAG_nnn'CTG Cuts at: 0 1224 2163 2296 2556 3012 5376 6148 6295 Size: 1224 939 133 260 456 2364 772 147 Cuts at: 6295 6408 Size: 113 Fragments arranged by size: 2364 1224 939 772 456 260 147 133 113 ApaI G_GGCC'C Cuts at: 0 4050 6408 Size: 4050 2358 ApaLI G'TGCA_C Cuts at: 0 1007 3562 6408 Size: 1007 2555 2846 Fragments arranged by size: 2846 2555 1007 ApoI r'AATT_y Cuts at: 0 3438 3970 6361 6408 Size: 3438 532 2391 47 Fragments arranged by size: 3438 2391 532 47 AvaI C'yCGr_G Cuts at: 0 326 779 1768 6408 Size: 326 453 989 4640 Fragments arranged by size: 4640 989 453 326 AvrII C'CTAG_G Cuts at: 0 5442 6408 Size: 5442 966 BamHI G'GATC_C Cuts at: 0 1297 1448 6408 Size: 1297 151 4960 Fragments arranged by size: 4960 1297 151 BanI G'GyrC_C Cuts at: 0 145 1585 2488 3129 3860 5430 5582 6298 Size: 145 1440 903 641 731 1570 152 716 Cuts at: 6298 6408 Size: 110 Fragments arranged by size: 1570 1440 903 731 716 641 152 145 110 BanII G_rGCy'C Cuts at: 0 2266 4050 4114 4871 5125 5492 6408 Size: 2266 1784 64 757 254 367 916 Fragments arranged by size: 2266 1784 916 757 367 254 64 BbeI G_GCGC'C Cuts at: 0 5434 6408 Size: 5434 974 BbsI GAAGACnn'nnnn_ Cuts at: 0 647 886 1531 2577 2929 3042 3520 4072 Size: 647 239 645 1046 352 113 478 552 Cuts at: 4072 5597 6408 Size: 1525 811 Fragments arranged by size: 1525 1046 811 647 645 552 478 352 239 113 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 39 73 799 833 3333 3367 5391 5425 Size: 39 34 726 34 2500 34 2024 34 Cuts at: 5425 6408 Size: 983 Fragments arranged by size: 2500 2024 983 726 39 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 0 4986 6408 Size: 4986 1422 BfrBI ATG'CAT Cuts at: 0 793 5196 6408 Size: 793 4403 1212 Fragments arranged by size: 4403 1212 793 BglII A'GATC_T Cuts at: 0 1167 2862 6408 Size: 1167 1695 3546 Fragments arranged by size: 3546 1695 1167 BlpI GC'TnA_GC Cuts at: 0 2265 2297 4870 5806 6408 Size: 2265 32 2573 936 602 Fragments arranged by size: 2573 2265 936 602 32 Bme1580I G_kGCm'C Cuts at: 0 148 1011 3315 3566 4050 6303 6408 Size: 148 863 2304 251 484 2253 105 Fragments arranged by size: 2304 2253 863 484 251 148 105 BmgBI CAC'GTC Cuts at: 0 2563 5049 5427 5664 6408 Size: 2563 2486 378 237 744 Fragments arranged by size: 2563 2486 744 378 237 BmrI ACTGGGnnnn_n' Cuts at: 0 1718 2539 2571 2959 3158 4992 6408 Size: 1718 821 32 388 199 1834 1416 Fragments arranged by size: 1834 1718 1416 821 388 199 32 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 5562 6408 Size: 5562 846 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 160 3124 3142 4822 5407 6142 6408 Size: 160 2964 18 1680 585 735 266 Fragments arranged by size: 2964 1680 735 585 266 160 18 Bpu10I CC'TnA_GC Cuts at: 0 4911 6408 Size: 4911 1497 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1498 2812 3706 4810 6408 Size: 1498 1314 894 1104 1598 Fragments arranged by size: 1598 1498 1314 1104 894 BsaI GGTCTCn'nnnn_ Cuts at: 0 325 1954 2020 3829 4225 6408 Size: 325 1629 66 1809 396 2183 Fragments arranged by size: 2183 1809 1629 396 325 66 BsaAI yAC'GTr Cuts at: 0 385 1996 6408 Size: 385 1611 4412 Fragments arranged by size: 4412 1611 385 BsaBI GATnn'nnATC Cuts at: 0 1490 6408 Size: 1490 4918 BsaHI Gr'CG_yC Cuts at: 0 4887 5431 6408 Size: 4887 544 977 Fragments arranged by size: 4887 977 544 BsaWI w'CCGG_w Cuts at: 0 86 3395 6408 Size: 86 3309 3013 Fragments arranged by size: 3309 3013 86 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 2614 2644 4417 4447 6408 Size: 2614 30 1773 30 1961 Fragments arranged by size: 2614 1961 1773 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 640 2635 3106 3109 3709 4422 6145 6408 Size: 640 1995 471 3 600 713 1723 263 Fragments arranged by size: 1995 1723 713 640 600 471 263 3 BseYI C'CCAG_C Cuts at: 0 480 1417 1519 1817 2556 2757 3839 4254 Size: 480 937 102 298 739 201 1082 415 Cuts at: 4254 4509 4952 6288 6408 Size: 255 443 1336 120 Fragments arranged by size: 1336 1082 937 739 480 443 415 298 255 201 120 102 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 628 696 1663 1746 2450 3013 3685 4097 Size: 628 68 967 83 704 563 672 412 Cuts at: 4097 4354 4558 4758 4760 6136 6408 Size: 257 204 200 2 1376 272 Fragments arranged by size: 1376 967 704 672 628 563 412 272 257 204 200 83 68 2 BsiHKAI G_wGCw'C Cuts at: 0 62 1011 1509 2538 3566 3749 4114 5492 Size: 62 949 498 1029 1028 183 365 1378 Cuts at: 5492 6408 Size: 916 Fragments arranged by size: 1378 1029 1028 949 916 498 365 183 62 BsmI GAATG_Cn' Cuts at: 0 2739 2789 4103 4424 6408 Size: 2739 50 1314 321 1984 Fragments arranged by size: 2739 1984 1314 321 50 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1474 4697 6408 Size: 1474 3223 1711 Fragments arranged by size: 3223 1711 1474 Bsp1286I G_dGCh'C Cuts at: 0 62 148 1011 1509 2266 2538 3315 3566 Size: 62 86 863 498 757 272 777 251 Cuts at: 3566 3749 4050 4114 4871 5125 5492 6303 6408 Size: 183 301 64 757 254 367 811 105 Fragments arranged by size: 863 811 777 757 757 498 367 301 272 254 251 183 105 86 64 62 BspEI T'CCGG_A Cuts at: 0 3395 6408 Size: 3395 3013 BspHI T'CATG_A Cuts at: 0 1089 2108 2817 6323 6408 Size: 1089 1019 709 3506 85 Fragments arranged by size: 3506 1089 1019 709 85 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 482 1209 3575 3917 4152 4865 5324 5684 Size: 482 727 2366 342 235 713 459 360 Cuts at: 5684 6408 Size: 724 Fragments arranged by size: 2366 727 724 713 482 459 360 342 235 BsrBI CCG'CTC Cuts at: 0 4446 6408 Size: 4446 1962 BsrDI GCAATG_nn' Cuts at: 0 1619 5161 5802 6152 6408 Size: 1619 3542 641 350 256 Fragments arranged by size: 3542 1619 641 350 256 BsrFI r'CCGG_y Cuts at: 0 230 3266 6408 Size: 230 3036 3142 Fragments arranged by size: 3142 3036 230 BsrGI T'GTAC_A Cuts at: 0 2498 4130 6408 Size: 2498 1632 2278 Fragments arranged by size: 2498 2278 1632 BssSI C'ACGA_G Cuts at: 0 2386 6408 Size: 2386 4022 BstAPI GCAn_nnn'nTGC Cuts at: 0 3117 3918 4084 6408 Size: 3117 801 166 2324 Fragments arranged by size: 3117 2324 801 166 BstEII G'GTnAC_C Cuts at: 0 5844 6408 Size: 5844 564 BstXI CCAn_nnnn'nTGG Cuts at: 0 1596 4497 6408 Size: 1596 2901 1911 Fragments arranged by size: 2901 1911 1596 BstYI r'GATC_y Cuts at: 0 1107 1167 1182 1297 1448 2325 2394 2835 Size: 1107 60 15 115 151 877 69 441 Cuts at: 2835 2862 4785 5015 6408 Size: 27 1923 230 1393 Fragments arranged by size: 1923 1393 1107 877 441 230 151 115 69 60 27 15 BstZ17I GTA'TAC Cuts at: 0 648 4017 4167 6408 Size: 648 3369 150 2241 Fragments arranged by size: 3369 2241 648 150 Bsu36I CC'TnA_GG Cuts at: 0 738 3407 5602 6408 Size: 738 2669 2195 806 Fragments arranged by size: 2669 2195 806 738 BtgI C'CryG_G Cuts at: 0 1178 1213 4052 4142 4622 4877 6408 Size: 1178 35 2839 90 480 255 1531 Fragments arranged by size: 2839 1531 1178 480 255 90 35 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 4326 6408 Size: 4326 2082 BtsI GCAGTG_nn' Cuts at: 0 2308 4524 4712 4770 6096 6408 Size: 2308 2216 188 58 1326 312 Fragments arranged by size: 2308 2216 1326 312 188 58 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 5839 5874 6408 Size: 5839 35 534 Fragments arranged by size: 5839 534 35 DraIII CAC_nnn'GTG Cuts at: 0 1015 6408 Size: 1015 5393 DrdI GACnn_nn'nnGTC Cuts at: 0 700 1946 6408 Size: 700 1246 4462 Fragments arranged by size: 4462 1246 700 EaeI y'GGCC_r Cuts at: 0 884 1001 1268 1676 1895 3264 4625 6408 Size: 884 117 267 408 219 1369 1361 1783 Fragments arranged by size: 1783 1369 1361 884 408 267 219 117 EarI CTCTTCn'nnn_ Cuts at: 0 195 659 1322 1727 4354 4479 4909 5497 Size: 195 464 663 405 2627 125 430 588 Cuts at: 5497 6169 6176 6408 Size: 672 7 232 Fragments arranged by size: 2627 672 663 588 464 430 405 232 195 125 7 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 19 6408 Size: 19 6389 EcoICRI GAG'CTC Cuts at: 0 4112 5490 6408 Size: 4112 1378 918 Fragments arranged by size: 4112 1378 918 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 160 1369 2341 3034 3124 3142 3613 4267 Size: 160 1209 972 693 90 18 471 654 Cuts at: 4267 4822 4890 5038 5407 5478 5899 5974 6142 Size: 555 68 148 369 71 421 75 168 Cuts at: 6142 6408 Size: 266 Fragments arranged by size: 1209 972 693 654 555 471 421 369 266 168 160 148 90 75 71 68 18 EcoNI CCTnn'n_nnAGG Cuts at: 0 2230 4861 5749 6408 Size: 2230 2631 888 659 Fragments arranged by size: 2631 2230 888 659 EcoO109I rG'GnC_Cy Cuts at: 0 1127 1236 1873 4046 4047 4149 5622 6145 Size: 1127 109 637 2173 1 102 1473 523 Cuts at: 6145 6408 Size: 263 Fragments arranged by size: 2173 1473 1127 637 523 263 109 102 1 EcoRV GAT'ATC Cuts at: 0 390 6408 Size: 390 6018 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2034 5154 5166 6294 6408 Size: 2034 3120 12 1128 114 Fragments arranged by size: 3120 2034 1128 114 12 HaeII r_GCGC'y Cuts at: 0 1674 2856 4125 5103 5434 6408 Size: 1674 1182 1269 978 331 974 Fragments arranged by size: 1674 1269 1182 978 974 331 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 106 138 1111 1143 1862 1894 5338 5370 Size: 106 32 973 32 719 32 3444 32 Cuts at: 5370 5519 5551 5710 5742 6199 6231 6408 Size: 149 32 159 32 457 32 177 Fragments arranged by size: 3444 973 719 457 177 159 149 106 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 2001 2376 6408 Size: 2001 375 4032 Fragments arranged by size: 4032 2001 375 HindIII A'AGCT_T Cuts at: 0 1886 2686 6408 Size: 1886 800 3722 Fragments arranged by size: 3722 1886 800 KasI G'GCGC_C Cuts at: 0 5430 6408 Size: 5430 978 MluI A'CGCG_T Cuts at: 0 5981 6408 Size: 5981 427 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 251 1962 2500 3530 3916 4776 5324 6254 Size: 251 1711 538 1030 386 860 548 930 Cuts at: 6254 6408 Size: 154 Fragments arranged by size: 1711 1030 930 860 548 538 386 251 154 MscI TGG'CCA Cuts at: 0 1003 1270 1678 1897 4627 6408 Size: 1003 267 408 219 2730 1781 Fragments arranged by size: 2730 1781 1003 408 267 219 MslI CAynn'nnrTG Cuts at: 0 151 955 1576 1594 1918 2494 3082 3527 Size: 151 804 621 18 324 576 588 445 Cuts at: 3527 3805 4141 4547 4963 5024 5207 5959 6408 Size: 278 336 406 416 61 183 752 449 Fragments arranged by size: 804 752 621 588 576 449 445 416 406 336 324 278 183 151 61 18 MspA1I CmG'CkG Cuts at: 0 406 807 915 1722 1821 2878 3009 3207 Size: 406 401 108 807 99 1057 131 198 Cuts at: 3207 3783 3821 3843 4061 5709 5886 5994 6120 Size: 576 38 22 218 1648 177 108 126 Cuts at: 6120 6408 Size: 288 Fragments arranged by size: 1648 1057 807 576 406 401 288 218 198 177 131 126 108 108 99 38 22 NarI GG'CG_CC Cuts at: 0 5431 6408 Size: 5431 977 NcoI C'CATG_G Cuts at: 0 1178 4142 4622 4877 6408 Size: 1178 2964 480 255 1531 Fragments arranged by size: 2964 1531 1178 480 255 NdeI CA'TA_TG Cuts at: 0 2064 6408 Size: 2064 4344 NsiI A_TGCA'T Cuts at: 0 795 5198 6408 Size: 795 4403 1210 Fragments arranged by size: 4403 1210 795 NspI r_CATG'y Cuts at: 0 1339 1551 2146 2223 2700 3366 3501 5502 Size: 1339 212 595 77 477 666 135 2001 Cuts at: 5502 5706 6408 Size: 204 702 Fragments arranged by size: 2001 1339 702 666 595 477 212 204 135 77 PciI A'CATG_T Cuts at: 0 2219 6408 Size: 2219 4189 PflMI CCAn_nnn'nTGG Cuts at: 0 570 1266 4509 4929 6295 6408 Size: 570 696 3243 420 1366 113 Fragments arranged by size: 3243 1366 696 570 420 113 PfoI T'CCnGG_A Cuts at: 0 1129 2179 2213 4190 6408 Size: 1129 1050 34 1977 2218 Fragments arranged by size: 2218 1977 1129 1050 34 PmlI CAC'GTG Cuts at: 0 385 6408 Size: 385 6023 PpuMI rG'GwC_Cy Cuts at: 0 1127 1236 1873 5622 6408 Size: 1127 109 637 3749 786 Fragments arranged by size: 3749 1127 786 637 109 PshAI GACnn'nnGTC Cuts at: 0 1871 6408 Size: 1871 4537 PspOMI G'GGCC_C Cuts at: 0 4046 6408 Size: 4046 2362 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 4171 4203 5290 5322 6408 Size: 4171 32 1087 32 1086 Fragments arranged by size: 4171 1087 1086 32 32 PstI C_TGCA'G Cuts at: 0 806 2552 3113 3572 3914 4109 4519 5321 Size: 806 1746 561 459 342 195 410 802 Cuts at: 5321 5687 6408 Size: 366 721 Fragments arranged by size: 1746 806 802 721 561 459 410 366 342 195 PvuII CAG'CTG Cuts at: 0 406 915 1722 1821 2878 3009 3207 3843 Size: 406 509 807 99 1057 131 198 636 Cuts at: 3843 5709 5886 5994 6120 6408 Size: 1866 177 108 126 288 Fragments arranged by size: 1866 1057 807 636 509 406 288 198 177 131 126 108 99 SacI G_AGCT'C Cuts at: 0 4114 5492 6408 Size: 4114 1378 916 Fragments arranged by size: 4114 1378 916 SapI GCTCTTCn'nnn_ Cuts at: 0 195 4479 4909 5497 6408 Size: 195 4284 430 588 911 Fragments arranged by size: 4284 911 588 430 195 SbfI CC_TGCA'GG Cuts at: 0 5321 5687 6408 Size: 5321 366 721 Fragments arranged by size: 5321 721 366 ScaI AGT'ACT Cuts at: 0 2632 3796 5002 6408 Size: 2632 1164 1206 1406 Fragments arranged by size: 2632 1406 1206 1164 SexAI A'CCwGG_T Cuts at: 0 1238 4976 6408 Size: 1238 3738 1432 Fragments arranged by size: 3738 1432 1238 SfcI C'TryA_G Cuts at: 0 802 2548 2626 3109 3568 3910 4105 4515 Size: 802 1746 78 483 459 342 195 410 Cuts at: 4515 5317 5683 6408 Size: 802 366 725 Fragments arranged by size: 1746 802 802 725 483 459 410 366 342 195 78 SfoI GGC'GCC Cuts at: 0 5432 6408 Size: 5432 976 SmlI C'TyrA_G Cuts at: 0 1477 2827 3721 4825 6408 Size: 1477 1350 894 1104 1583 Fragments arranged by size: 1583 1477 1350 1104 894 SphI G_CATG'C Cuts at: 0 1551 3501 5706 6408 Size: 1551 1950 2205 702 Fragments arranged by size: 2205 1950 1551 702 StuI AGG'CCT Cuts at: 0 5773 6408 Size: 5773 635 StyI C'CwwG_G Cuts at: 0 1178 1251 1637 2009 2588 2738 4142 4490 Size: 1178 73 386 372 579 150 1404 348 Cuts at: 4490 4565 4622 4877 5442 5519 5864 6263 6408 Size: 75 57 255 565 77 345 399 145 Fragments arranged by size: 1404 1178 579 565 399 386 372 348 345 255 150 145 77 75 73 57 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1831 2393 2506 5789 5863 6183 6408 Size: 1831 562 113 3283 74 320 225 Fragments arranged by size: 3283 1831 562 320 225 113 74 TatI w'GTAC_w Cuts at: 0 35 2498 2630 3794 4130 5000 6317 6356 Size: 35 2463 132 1164 336 870 1317 39 Cuts at: 6356 6408 Size: 52 Fragments arranged by size: 2463 1317 1164 870 336 132 52 39 35 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 374 406 6408 Size: 374 32 6002 Fragments arranged by size: 6002 374 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 367 1635 3594 3623 4149 4458 5005 6270 Size: 367 1268 1959 29 526 309 547 1265 Cuts at: 6270 6408 Size: 138 Fragments arranged by size: 1959 1268 1265 547 526 367 309 138 29 XmnI GAAnn'nnTTC Cuts at: 0 3439 5134 6408 Size: 3439 1695 1274 Fragments arranged by size: 3439 1695 1274 Enzymes that do cut: AarI AccI AclI AcuI AflIII AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I HincII HindIII KasI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NsiI NspI PciI PflMI PfoI PmlI PpuMI PshAI PspOMI PsrI PstI PvuII SacI SapI SbfI ScaI SexAI SfcI SfoI SmlI SphI StuI StyI TaqII TatI TstI XcmI XmnI Enzymes that do not cut: AatII Acc65I AfeI AflII AgeI AloI AscI AseI AsiSI BaeI BbvCI BclI BglI BmtI BsiEI BsiWI BssHII BstBI ClaI DraI EagI EcoRI FseI FspI FspAI HpaI KpnI MfeI NaeI NgoMIV NheI NotI NruI PacI PasI PmeI PpiI PsiI PspXI PvuI RsrII SacII SalI SanDI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XhoI XmaI ZraI