(Circular) (Six-base) MAPSORT of: pFN21AB5948.seq Check: 1201 from: 1 to: 6337 pFN21AB5948 6337 bp With 182 enzymes: SgfI * November 21, 2014 12:14 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4147 278 Size: 53 83 186 936 2611 2468 Fragments arranged by size: 2611 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3680 1937 Size: 1743 4594 Acc65I G'GTAC_C Cuts at: 3658 3658 Size: 6337 AclI AA'CG_TT Cuts at: 1563 4464 4837 1563 Size: 2901 373 3063 Fragments arranged by size: 3063 2901 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2064 2142 2208 2538 4400 5784 6052 1632 Size: 432 78 66 330 1862 1384 268 1917 Fragments arranged by size: 1917 1862 1384 432 330 268 78 66 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6140 AflIII A'CryG_T Cuts at: 1203 5236 6213 1203 Size: 4033 977 1327 Fragments arranged by size: 4033 1327 977 AgeI A'CCGG_T Cuts at: 5168 5168 Size: 6337 AhdI GACnn_n'nnGTC Cuts at: 5066 5066 Size: 6337 AleI CACnn'nnGTG Cuts at: 1166 3269 1166 Size: 2103 4234 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3121 1184 Size: 1937 4400 AlwNI CAG_nnn'CTG Cuts at: 2489 2846 4080 5652 2489 Size: 357 1234 1572 3174 Fragments arranged by size: 3174 1572 1234 357 ApaLI G'TGCA_C Cuts at: 2442 4394 5550 2442 Size: 1952 1156 3229 Fragments arranged by size: 3229 1952 1156 ApoI r'AATT_y Cuts at: 1493 2273 2283 2375 2943 3646 3872 4024 6133 Size: 780 10 92 568 703 226 152 2109 Cuts at: 6133 1493 Size: 1697 Fragments arranged by size: 2109 1697 780 703 568 226 152 92 10 AseI AT'TA_AT Cuts at: 160 1032 2930 2937 4891 160 Size: 872 1898 7 1954 1606 Fragments arranged by size: 1954 1898 1606 872 7 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6337 AvaI C'yCGr_G Cuts at: 1870 1943 3188 3662 1870 Size: 73 1245 474 4545 Fragments arranged by size: 4545 1245 474 73 BamHI G'GATC_C Cuts at: 3667 4034 3667 Size: 367 5970 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3292 3658 5113 618 Size: 325 217 627 1505 366 1455 1842 Fragments arranged by size: 1842 1505 1455 627 366 325 217 BanII G_rGCy'C Cuts at: 1391 3450 3656 1391 Size: 2059 206 4072 Fragments arranged by size: 4072 2059 206 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5366 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4528 4562 1903 Size: 34 2591 34 3678 Fragments arranged by size: 3678 2591 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2444 4229 5439 2444 Size: 1785 1210 3342 Fragments arranged by size: 3342 1785 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6337 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4948 136 Size: 107 122 71 631 605 3276 1525 Fragments arranged by size: 3276 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2211 2754 6332 2211 Size: 543 3578 2216 Fragments arranged by size: 3578 2216 543 BlpI GC'TnA_GC Cuts at: 3754 3754 Size: 6337 Bme1580I G_kGCm'C Cuts at: 1790 2302 2446 3235 4398 5554 1790 Size: 512 144 789 1163 1156 2573 Fragments arranged by size: 2573 1163 1156 789 512 144 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6337 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4068 5026 442 Size: 416 53 83 399 24 2651 958 1753 Fragments arranged by size: 2651 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6337 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3426 3434 3565 4997 1239 Size: 2187 8 131 1432 2579 Fragments arranged by size: 2579 2187 1432 131 8 Bpu10I CC'TnA_GC Cuts at: 3246 3246 Size: 6337 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3524 4462 5327 5625 3524 Size: 938 865 298 4236 Fragments arranged by size: 4236 938 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3065 3348 5000 882 Size: 617 1566 283 1652 2219 Fragments arranged by size: 2219 1652 1566 617 283 BsaAI yAC'GTr Cuts at: 493 1204 4128 493 Size: 711 2924 2702 Fragments arranged by size: 2924 2702 711 BsaBI GATnn'nnATC Cuts at: 6331 6331 Size: 6337 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4144 4526 5161 275 Size: 53 83 186 936 2611 382 635 1451 Fragments arranged by size: 2611 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4770 5168 5442 5589 6000 4770 Size: 398 274 147 411 5107 Fragments arranged by size: 5107 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2081 2111 2292 2322 1178 Size: 30 165 30 678 30 181 30 5193 Fragments arranged by size: 5193 678 181 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2034 2181 2765 3582 1186 Size: 848 147 584 817 3941 Fragments arranged by size: 3941 848 817 584 147 BseYI C'CCAG_C Cuts at: 5540 6036 5540 Size: 496 5841 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3122 1163 Size: 573 1386 4378 Fragments arranged by size: 4378 1386 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3787 4548 5576 1540 Size: 275 186 1786 761 1028 2301 Fragments arranged by size: 2301 1786 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2446 3450 4398 4483 5554 2446 Size: 1004 948 85 1071 3229 Fragments arranged by size: 3229 1071 1004 948 85 BsmI GAATG_Cn' Cuts at: 3848 3941 3848 Size: 93 6244 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2302 2446 3235 3450 3656 4398 4483 Size: 399 512 144 789 215 206 742 85 Cuts at: 4483 5554 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 789 742 512 399 215 206 144 85 BspEI T'CCGG_A Cuts at: 6000 6000 Size: 6337 BspHI T'CATG_A Cuts at: 2952 4226 5956 2952 Size: 1274 1730 3333 Fragments arranged by size: 3333 1730 1274 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3692 844 Size: 2848 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4224 1394 Size: 429 2401 3507 Fragments arranged by size: 3507 2401 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2300 2631 2865 4832 5006 65 Size: 1183 1052 331 234 1967 174 1396 Fragments arranged by size: 1967 1396 1183 1052 331 234 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4981 5168 1700 Size: 110 3171 187 2869 Fragments arranged by size: 3171 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2703 96 Size: 2607 3730 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6337 BssSI C'ACGA_G Cuts at: 4397 5409 4397 Size: 1012 5325 BstAPI GCAn_nnn'nTGC Cuts at: 794 3114 794 Size: 2320 4017 BstBI TT'CG_AA Cuts at: 5141 5141 Size: 6337 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6337 BstXI CCAn_nnnn'nTGG Cuts at: 2295 2295 Size: 6337 BstYI r'GATC_y Cuts at: 1972 2211 2754 3527 3667 4034 4419 4436 5888 Size: 239 543 773 140 367 385 17 1452 Cuts at: 5888 5974 5986 6332 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 773 543 385 367 346 239 140 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3297 3297 Size: 6337 BtgI C'CryG_G Cuts at: 513 1065 2112 513 Size: 552 1047 4738 Fragments arranged by size: 4738 1047 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5598 Fragments arranged by size: 5598 683 56 BtsI GCAGTG_nn' Cuts at: 3853 4647 4667 3853 Size: 794 20 5523 Fragments arranged by size: 5523 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6337 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6302 DraI TTT'AAA Cuts at: 3492 3561 3641 3492 Size: 69 80 6188 Fragments arranged by size: 6188 80 69 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5344 809 Size: 507 4028 1802 Fragments arranged by size: 4028 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2886 3784 4673 Size: 54 1425 50 275 18 1056 898 889 Cuts at: 4673 6302 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1056 898 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3784 1537 Size: 275 1972 4090 Fragments arranged by size: 4090 1972 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2022 2118 2223 2526 4267 1353 Size: 669 96 105 303 1741 3423 Fragments arranged by size: 3423 1741 669 303 105 96 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4924 5298 5444 387 Size: 1064 138 3335 374 146 1280 Fragments arranged by size: 3335 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3448 3448 Size: 6337 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2064 2142 2208 2538 3426 3434 3565 Size: 393 432 78 66 330 888 8 131 Cuts at: 3565 4400 4997 5784 6052 1239 Size: 835 597 787 268 1524 Fragments arranged by size: 1524 888 835 787 597 432 393 330 268 131 78 66 8 EcoO109I rG'GnC_Cy Cuts at: 2791 2791 Size: 6337 EcoRI G'AATT_C Cuts at: 2273 2283 3646 4024 2273 Size: 10 1363 378 4586 Fragments arranged by size: 4586 1363 378 10 EcoRV GAT'ATC Cuts at: 3337 3337 Size: 6337 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2162 2162 Size: 6337 FspI TGC'GCA Cuts at: 4843 4843 Size: 6337 HaeII r_GCGC'y Cuts at: 1678 2206 5484 1678 Size: 528 3278 2531 Fragments arranged by size: 3278 2531 528 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3326 3358 3452 3484 4978 Size: 32 135 32 1754 32 94 32 1494 Cuts at: 4978 5010 5052 5084 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1754 1494 135 94 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2401 3681 3927 669 Size: 988 281 463 1280 246 3079 Fragments arranged by size: 3079 1280 988 463 281 246 HindIII A'AGCT_T Cuts at: 748 3123 3315 748 Size: 2375 192 3770 Fragments arranged by size: 3770 2375 192 HpaI GTT'AAC Cuts at: 3927 3927 Size: 6337 KpnI G_GTAC'C Cuts at: 3662 3662 Size: 6337 MfeI C'AATT_G Cuts at: 3360 3936 3360 Size: 576 5761 MluI A'CGCG_T Cuts at: 6213 6213 Size: 6337 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2343 5451 5635 1305 Size: 1038 3108 184 2007 Fragments arranged by size: 3108 2007 1038 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2888 10 Size: 54 1425 343 1056 3459 Fragments arranged by size: 3459 1425 1056 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3269 4295 4654 4813 518 Size: 648 2103 1026 359 159 2042 Fragments arranged by size: 2103 2042 1026 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3101 3473 3753 4430 5578 5823 1606 Size: 1495 372 280 677 1148 245 2120 Fragments arranged by size: 2120 1495 1148 677 372 280 245 NcoI C'CATG_G Cuts at: 513 1065 2112 513 Size: 552 1047 4738 Fragments arranged by size: 4738 1047 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6337 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6337 NotI GC'GGCC_GC Cuts at: 3784 3784 Size: 6337 NspI r_CATG'y Cuts at: 1132 1229 3695 5240 1132 Size: 97 2466 1545 2229 Fragments arranged by size: 2466 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6337 PciI A'CATG_T Cuts at: 5236 5236 Size: 6337 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6337 PmeI GTTT'AAAC Cuts at: 3641 3641 Size: 6337 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4406 4438 5919 5951 4406 Size: 32 1481 32 4792 Fragments arranged by size: 4792 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2791 2791 Size: 6337 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6337 PsiI TTA'TAA Cuts at: 3907 3907 Size: 6337 PstI C_TGCA'G Cuts at: 830 3110 3689 830 Size: 2280 579 3478 Fragments arranged by size: 3478 2280 579 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6337 PvuII CAG'CTG Cuts at: 3101 3473 3101 Size: 372 5965 SacI G_AGCT'C Cuts at: 3450 3450 Size: 6337 SalI G'TCGA_C Cuts at: 1936 3679 1936 Size: 1743 4594 SanDI GG'GwC_CC Cuts at: 2791 2791 Size: 6337 SbfI CC_TGCA'GG Cuts at: 3689 3689 Size: 6337 ScaI AGT'ACT Cuts at: 2698 4585 6297 2698 Size: 1887 1712 2738 Fragments arranged by size: 2738 1887 1712 SexAI A'CCwGG_T Cuts at: 3147 3147 Size: 6337 SfcI C'TryA_G Cuts at: 826 1045 3106 3685 4820 5501 5692 826 Size: 219 2061 579 1135 681 191 1471 Fragments arranged by size: 2061 1471 1135 681 579 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6337 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6337 SmaI CCC'GGG Cuts at: 1872 3664 1872 Size: 1792 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3503 4441 5342 5604 820 Size: 197 926 1560 938 901 262 1553 Fragments arranged by size: 1560 1553 938 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4128 493 Size: 3635 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6337 SphI G_CATG'C Cuts at: 1132 3695 1132 Size: 2563 3774 SspI AAT'ATT Cuts at: 5 52 2977 3181 4261 5 Size: 47 2925 204 1080 2081 Fragments arranged by size: 2925 2081 1080 204 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6337 StyI C'CwwG_G Cuts at: 513 1065 2112 3534 3776 513 Size: 552 1047 1422 242 3074 Fragments arranged by size: 3074 1422 1047 552 242 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2996 4364 4381 4534 4719 5164 325 Size: 1255 1416 1368 17 153 185 445 1498 Fragments arranged by size: 1498 1416 1368 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2474 2696 2703 Size: 274 80 33 51 1443 497 222 7 Cuts at: 2703 4583 6295 96 Size: 1880 1712 138 Fragments arranged by size: 1880 1712 1443 497 274 222 138 80 51 33 7 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2269 2292 2301 2324 2868 2900 6297 6329 2269 Size: 23 9 23 544 32 3397 32 2277 Fragments arranged by size: 3397 2277 544 32 32 23 23 9 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6337 XbaI T'CTAG_A Cuts at: 2362 3673 2362 Size: 1311 5026 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6337 XmaI C'CCGG_G Cuts at: 1870 3662 1870 Size: 1792 4545 XmnI GAAnn'nnTTC Cuts at: 4466 4466 Size: 6337 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4145 276 Size: 53 83 186 936 2611 2468 Fragments arranged by size: 2611 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SapI SfiI SfoI SrfI SwaI XcmI