################################## # NO CUT ENZYMES ( 117 enzymes) # ################################## AatII Acc65I AccII AclI AcyI AflII AflIII AgeI AocI Aor51HI AscI AseI AsnI Asp700I Asp718I AspI AvaI AviII BalI BanI BbeI BbrPI BbvCI BcgI BciVI BfrI BglI BglII BlpI Bpu1102I BsaAI BsaBI BsaHI BsiWI BsmI BsoBI Bsp1407I BspHI BspLU11I BspMI BsrBI BsrGI BssHII BstEII BstPI BstUI Bsu36I CelII CfoI CpoI DraIII DrdI Eco47III Eco81I EcoO65I EcoT22I FseI FspI HaeII HhaI Hin1I HinP1I HpaI KasI KpnI KspI MamI MfeI MluI MluNI MscI MunI MvnI NarI NdeI NheI NotI NruI NsiI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AccI 2315 GTATAC GT^[AC][GT]AC AhdI 428 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 20 GTGCAC G^TGCAC AlwNI 1448 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 2059 GGGCCC GGGCC^C ApaLI 20 GTGCAC G^TGCAC AspEI 428 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 1149 CCTAGG C^CTAGG BamHI 1585 GGATCC G^GATCC BclI 75 TGATCA T^GATCA BlnI 1149 CCTAGG C^CTAGG BsiEI 2041 CGGCCG CG[GA][CT]^CG BsmBI 1236 CGTCTC CGTCTC(1/5) BspDI 47 ATCGAT AT^CGAT BsrDI 2373 CATTGC GCAATG(2/0) Bst1107I 2315 GTATAC GTA^TAC BstAPI 1447 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 1869 TTCGAA TT^CGAA BstZ17I 2315 GTATAC GTA^TAC ClaI 47 ATCGAT AT^CGAT EagI 2041 CGGCCG C^GGCCG Eam1105I 428 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 2041 CGGCCG C^GGCCG Eco52I 2041 CGGCCG C^GGCCG EcoNI 1972 CCTGAACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FauI 453 GCGGG CCCGC(4/6) FbaI 75 TGATCA T^GATCA MspA1I 1448 CAGCTG C[AC]G^C[GT]G NaeI 2239 GCCGGC GCC^GGC NcoI 165 CCATGG C^CATGG NgoMIV 2239 GCCGGC G^CCGGC NspV 1869 TTCGAA TT^CGAA PstI 410 CTGCAG CTGCA^G PvuII 1448 CAGCTG CAG^CTG SapI 2563 GAAGAGC GCTCTTC(1/4) SfaNI 1432 GCATC GCATC(5/9) SfuI 1869 TTCGAA TT^CGAA ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AfaI 978 GTAC GT^AC 2247 GTAC BcnI 1577 CCGGG CC^[GC]GG 2039 CCCGG BfaI 1150 CTAG C^TAG 2476 CTAG BsmFI 265 GGGAC GGGAC(10/14) 343 GGGAC BsrFI 2043 GCCGGT [GA]^CCGG[CT] 2239 GCCGGC BssSI 1438 CACGAG CACGAG(-5/-1) 121 CTCGTG BstXI 1283 CCACTGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2393 CCAGGTCTCTGG BstYI 1397 AGATCC [GA]^GATC[CT] 1585 GGATCC Cfr10I 2043 GCCGGT [GA]^CCGG[CT] 2239 GCCGGC DraI 819 TTTAAA TTT^AAA 1684 TTTAAA DraII 594 AGGCCCT [GA]G^G[ACGT]CC[CT] 2058 GGGGCCC DsaI 165 CCATGG C^C[GA][CT]GG 1425 CCGTGG EaeI 2041 CGGCCG [CT]^GGCC[GA] 2241 CGGCCA EarI 775 GAAGAG CTCTTC(1/4) 2563 GAAGAG EcoO109I 594 AGGCCCT [GA]G^G[ACGT]CC[CT] 2058 GGGGCCC EcoRI 312 GAATTC G^AATTC 2098 GAATTC HgaI 64 GACGC GACGC(5/10) 2427 GCGTC HincII 226 GTCAAC GT[CT]^[GA]AC 1006 GTTGAC HindII 226 GTCAAC GT[CT]^[GA]AC 1006 GTTGAC HindIII 1113 AAGCTT A^AGCTT 2551 AAGCTT Ksp632I 775 GAAGAG CTCTTC(1/4) 2563 GAAGAG MaeI 1150 CTAG C^TAG 2476 CTAG MflI 1397 AGATCC [GA]^GATC[CT] 1585 GGATCC MslI 166 CATGGATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 843 CATTTATATG NciI 1577 CCGGG CC^[GC]GG 2039 CCCGG RsaI 978 GTAC GT^AC 2247 GTAC SacI 1378 GAGCTC GAGCT^C 1572 GAGCTC SfcI 410 CTGCAG C^T[GA][CT]AG 750 CTACAG SspI 789 AATATT AAT^ATT 2495 AATATT StuI 1024 AGGCCT AGG^CCT 1152 AGGCCT Tsp45I 1106 GTGAC ^GT[GC]AC 1480 GTGAC XhoII 1397 AGATCC [GA]^GATC[CT] 1585 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 72 enzymes) # ######################################### AccIII AciI AcsI AluI AlwI ApoI AspHI AvaII BanII BbsI BbvI BmyI BpmI BpuAI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiHKAI BsiYI BslI BsmAI Bsp1286I BspEI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EcoRII EcoRV EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MroI MseI MspI MvaI MwoI NdeII NlaIII NlaIV NspI PleI Sau3AI Sau96I ScrFI StyI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XcmI