(Circular) (Six-base) MAPSORT of: pFN21AE9663.seq Check: 1502 from: 1 to: 5821 pFN21AE9663 5821 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2548 2548 Size: 5821 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2045 3631 278 Size: 53 83 186 936 509 1586 2468 Fragments arranged by size: 2468 1586 936 509 186 83 53 AccI GT'mk_AC Cuts at: 1937 3023 3164 1937 Size: 1086 141 4594 Fragments arranged by size: 4594 1086 141 Acc65I G'GTAC_C Cuts at: 3142 3142 Size: 5821 AclI AA'CG_TT Cuts at: 1563 3120 3948 4321 1563 Size: 1557 828 373 3063 Fragments arranged by size: 3063 1557 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3017 3884 5268 5536 1632 Size: 1385 867 1384 268 1917 Fragments arranged by size: 1917 1385 1384 867 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5624 AflIII A'CryG_T Cuts at: 1203 4720 5697 1203 Size: 3517 977 1327 Fragments arranged by size: 3517 1327 977 AgeI A'CCGG_T Cuts at: 4652 4652 Size: 5821 AhdI GACnn_n'nnGTC Cuts at: 4550 4550 Size: 5821 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5821 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3107 1184 Size: 1923 3898 AlwNI CAG_nnn'CTG Cuts at: 2593 3564 5136 2593 Size: 971 1572 3278 Fragments arranged by size: 3278 1572 971 ApaI G_GGCC'C Cuts at: 2470 2766 2470 Size: 296 5525 ApaLI G'TGCA_C Cuts at: 3878 5034 3878 Size: 1156 4665 ApoI r'AATT_y Cuts at: 1493 3130 3356 3508 5617 1493 Size: 1637 226 152 2109 1697 Fragments arranged by size: 2109 1697 1637 226 152 AseI AT'TA_AT Cuts at: 160 1032 4375 160 Size: 872 3343 1606 Fragments arranged by size: 3343 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5821 AvaI C'yCGr_G Cuts at: 1870 1943 2336 3146 1870 Size: 73 393 810 4545 Fragments arranged by size: 4545 810 393 73 AvrII C'CTAG_G Cuts at: 2689 2689 Size: 5821 BamHI G'GATC_C Cuts at: 2360 3151 3518 2360 Size: 791 367 4663 Fragments arranged by size: 4663 791 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2137 3142 4597 618 Size: 325 217 627 350 1005 1455 1842 Fragments arranged by size: 1842 1455 1005 627 350 325 217 BanII G_rGCy'C Cuts at: 1391 2470 2766 3140 1391 Size: 1079 296 374 4072 Fragments arranged by size: 4072 1079 374 296 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4850 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4012 4046 1903 Size: 34 2075 34 3678 Fragments arranged by size: 3678 2075 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3713 4923 3713 Size: 1210 4611 BclI T'GATC_A Cuts at: 1548 1548 Size: 5821 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2408 4432 136 Size: 107 122 71 631 605 736 2024 1525 Fragments arranged by size: 2024 1525 736 631 605 122 107 71 BglII A'GATC_T Cuts at: 5816 5816 Size: 5821 BlpI GC'TnA_GC Cuts at: 2985 3238 2985 Size: 253 5568 Bme1580I G_kGCm'C Cuts at: 1790 2140 2470 2537 2766 3882 5038 1790 Size: 350 330 67 229 1116 1156 2573 Fragments arranged by size: 2573 1156 1116 350 330 229 67 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5821 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3552 4510 442 Size: 416 53 83 399 24 2135 958 1753 Fragments arranged by size: 2135 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2348 1056 Size: 1292 4529 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2207 2723 2799 2992 4481 1239 Size: 968 516 76 193 1489 2579 Fragments arranged by size: 2579 1489 968 516 193 76 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3946 4811 5109 3946 Size: 865 298 4658 Fragments arranged by size: 4658 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4484 882 Size: 617 2985 2219 Fragments arranged by size: 2985 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3612 493 Size: 711 2408 2702 Fragments arranged by size: 2702 2408 711 BsaBI GATnn'nnATC Cuts at: 5815 5815 Size: 5821 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2042 3114 3628 4010 Size: 53 83 186 936 509 1072 514 382 Cuts at: 4010 4645 275 Size: 635 1451 Fragments arranged by size: 1451 1072 936 635 514 509 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2748 4254 4652 4926 5073 5484 2748 Size: 1506 398 274 147 411 3085 Fragments arranged by size: 3085 1506 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2772 2802 1178 Size: 30 165 30 1369 30 4197 Fragments arranged by size: 4197 1369 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5821 BseYI C'CCAG_C Cuts at: 2398 5024 5520 2398 Size: 2626 496 2699 Fragments arranged by size: 2699 2626 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2871 3113 1163 Size: 573 1135 242 3871 Fragments arranged by size: 3871 1135 573 242 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3271 4032 5060 1540 Size: 275 186 1270 761 1028 2301 Fragments arranged by size: 2301 1270 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3882 3967 5038 3882 Size: 85 1071 4665 Fragments arranged by size: 4665 1071 85 BsmI GAATG_Cn' Cuts at: 3332 3425 3332 Size: 93 5728 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2140 2470 2537 2766 3140 3882 3967 Size: 399 350 330 67 229 374 742 85 Cuts at: 3967 5038 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 374 350 330 229 85 67 BspEI T'CCGG_A Cuts at: 2748 5484 2748 Size: 2736 3085 BspHI T'CATG_A Cuts at: 3710 5440 3710 Size: 1730 4091 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2548 3176 844 Size: 1704 628 3489 Fragments arranged by size: 3489 1704 628 BsrBI CCG'CTC Cuts at: 1394 1823 3708 1394 Size: 429 1885 3507 Fragments arranged by size: 3507 1885 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2206 2928 4316 4490 65 Size: 1183 958 722 1388 174 1396 Fragments arranged by size: 1396 1388 1183 958 722 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2627 4465 4652 1700 Size: 110 817 1838 187 2869 Fragments arranged by size: 2869 1838 817 187 110 BsrGI T'GTAC_A Cuts at: 96 2145 96 Size: 2049 3772 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5821 BssSI C'ACGA_G Cuts at: 3881 4893 3881 Size: 1012 4809 BstAPI GCAn_nnn'nTGC Cuts at: 794 2248 794 Size: 1454 4367 BstBI TT'CG_AA Cuts at: 4625 4625 Size: 5821 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5821 BstYI r'GATC_y Cuts at: 1972 2360 3151 3518 3903 3920 5372 5458 5470 Size: 388 791 367 385 17 1452 86 12 Cuts at: 5470 5816 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 791 388 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2014 2076 2800 513 Size: 552 949 62 724 3534 Fragments arranged by size: 3534 949 724 552 62 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5082 Fragments arranged by size: 5082 683 56 BtsI GCAGTG_nn' Cuts at: 3337 4131 4151 3337 Size: 794 20 5007 Fragments arranged by size: 5007 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5821 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5786 DraI TTT'AAA Cuts at: 3125 3125 Size: 5821 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2047 4828 809 Size: 507 731 2781 1802 Fragments arranged by size: 2781 1802 731 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2151 2259 2584 Size: 54 1425 50 275 18 321 108 325 Cuts at: 2584 3102 3268 4157 5786 8 Size: 518 166 889 1629 43 Fragments arranged by size: 1629 1425 889 518 325 321 275 166 108 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3268 1537 Size: 275 1456 4090 Fragments arranged by size: 4090 1456 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2265 2582 3040 3751 1353 Size: 912 317 458 711 3423 Fragments arranged by size: 3423 912 711 458 317 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2098 4408 4782 4928 387 Size: 1064 138 509 2310 374 146 1280 Fragments arranged by size: 2310 1280 1064 509 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2207 2723 2799 2992 3017 3884 4481 Size: 393 575 516 76 193 25 867 597 Cuts at: 4481 5268 5536 1239 Size: 787 268 1524 Fragments arranged by size: 1524 867 787 597 575 516 393 268 193 76 25 EcoO109I rG'GnC_Cy Cuts at: 2466 2763 2466 Size: 297 5524 EcoRI G'AATT_C Cuts at: 3130 3508 3130 Size: 378 5443 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2593 2593 Size: 5821 FspI TGC'GCA Cuts at: 4327 4327 Size: 5821 HaeII r_GCGC'y Cuts at: 1678 2517 4968 1678 Size: 839 2451 2531 Fragments arranged by size: 2531 2451 839 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2772 2804 2968 3000 4462 Size: 32 135 32 1200 32 164 32 1462 Cuts at: 4462 4494 4536 4568 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1462 1200 164 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3165 3411 669 Size: 988 281 1227 246 3079 Fragments arranged by size: 3079 1227 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5821 HpaI GTT'AAC Cuts at: 3411 3411 Size: 5821 KpnI G_GTAC'C Cuts at: 3146 3146 Size: 5821 MfeI C'AATT_G Cuts at: 3420 3420 Size: 5821 MluI A'CGCG_T Cuts at: 5697 5697 Size: 5821 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2355 4935 5119 1305 Size: 1050 2580 184 2007 Fragments arranged by size: 2580 2007 1050 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2261 2586 3104 10 Size: 54 1425 343 429 325 518 2727 Fragments arranged by size: 2727 1425 518 429 343 325 54 MslI CAynn'nnrTG Cuts at: 518 1166 3089 3779 4138 4297 518 Size: 648 1923 690 359 159 2042 Fragments arranged by size: 2042 1923 690 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2016 2177 2284 2952 2989 3237 3914 5062 Size: 410 161 107 668 37 248 677 1148 Cuts at: 5062 5307 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 668 410 248 245 161 107 37 NaeI GCC'GGC Cuts at: 2629 2629 Size: 5821 NcoI C'CATG_G Cuts at: 513 1065 2800 513 Size: 552 1735 3534 Fragments arranged by size: 3534 1735 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5821 NgoMIV G'CCGG_C Cuts at: 2627 2627 Size: 5821 NheI G'CTAG_C Cuts at: 1052 2344 1052 Size: 1292 4529 NotI GC'GGCC_GC Cuts at: 3268 3268 Size: 5821 NspI r_CATG'y Cuts at: 1132 1229 3179 4724 1132 Size: 97 1950 1545 2229 Fragments arranged by size: 2229 1950 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5821 PciI A'CATG_T Cuts at: 4720 4720 Size: 5821 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5821 PfoI T'CCnGG_A Cuts at: 2160 2739 2160 Size: 579 5242 PmeI GTTT'AAAC Cuts at: 3125 3125 Size: 5821 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3890 3922 5403 5435 3890 Size: 32 1481 32 4276 Fragments arranged by size: 4276 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5821 PsiI TTA'TAA Cuts at: 3391 3391 Size: 5821 PspOMI G'GGCC_C Cuts at: 2466 2762 2466 Size: 296 5525 PstI C_TGCA'G Cuts at: 830 3173 830 Size: 2343 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5821 PvuII CAG'CTG Cuts at: 2284 2989 2284 Size: 705 5116 SacII CC_GC'GG Cuts at: 2017 2017 Size: 5821 SalI G'TCGA_C Cuts at: 1936 3163 1936 Size: 1227 4594 SapI GCTCTTCn'nnn_ Cuts at: 2265 2265 Size: 5821 SbfI CC_TGCA'GG Cuts at: 3173 3173 Size: 5821 ScaI AGT'ACT Cuts at: 4069 5781 4069 Size: 1712 4109 SfcI C'TryA_G Cuts at: 826 1045 3169 4304 4985 5176 826 Size: 219 2124 1135 681 191 1471 Fragments arranged by size: 2124 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5821 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5821 SmaI CCC'GGG Cuts at: 1872 3148 1872 Size: 1276 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3925 4826 5088 820 Size: 197 926 1982 901 262 1553 Fragments arranged by size: 1982 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3612 493 Size: 3119 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5821 SphI G_CATG'C Cuts at: 1132 3179 1132 Size: 2047 3774 SspI AAT'ATT Cuts at: 5 52 3745 5 Size: 47 3693 2081 Fragments arranged by size: 3693 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5821 StyI C'CwwG_G Cuts at: 513 1065 2581 2689 2766 2800 3260 513 Size: 552 1516 108 77 34 460 3074 Fragments arranged by size: 3074 1516 552 460 108 77 34 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3848 3865 4018 4203 4648 325 Size: 1255 2268 17 153 185 445 1498 Fragments arranged by size: 2268 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2118 2145 3051 Size: 274 80 33 51 1443 141 27 906 Cuts at: 3051 4067 5779 96 Size: 1016 1712 138 Fragments arranged by size: 1712 1443 1016 906 274 141 138 80 51 33 27 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2868 2900 5781 5813 2868 Size: 32 2881 32 2876 Fragments arranged by size: 2881 2876 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5821 XbaI T'CTAG_A Cuts at: 3157 3157 Size: 5821 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2371 2371 Size: 5821 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5821 XmaI C'CCGG_G Cuts at: 1870 3146 1870 Size: 1276 4545 XmnI GAAnn'nnTTC Cuts at: 2868 3950 2868 Size: 1082 4739 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2043 3629 276 Size: 53 83 186 936 509 1586 2468 Fragments arranged by size: 2468 1586 936 509 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SanDI SexAI SfiI SfoI SrfI SwaI