################################## # NO CUT ENZYMES ( 108 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AflII AgeI AhdI Alw44I ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI BalI BbrPI BcgI BciVI BclI BfrI BglII BsaAI BsmBI BsmI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DrdI Eam1105I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HinfI HpaI KpnI MfeI MluNI MscI MseI MslI MunI NcoI NdeI NheI NlaIII NruI NsiI NspI NspV PacI PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tth111I Van91I XbaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccIII 437 TCCGGA T^CCGGA AfaI 150 GTAC GT^AC AflIII 152 ACGCGT A^C[GA][CT]GT AocI 1013 CCTCAGG CC^T[ACGT]AGG AviII 752 TGCGCA TGC^GCA AvrII 651 CCTAGG C^CTAGG BanI 236 GGCGCC G^G[CT][GA]CC BbeI 236 GGCGCC GGCGC^C BbvCI 804 GCTGAGG CCTCAGC(-5/-2) BglI 223 GCCGAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 651 CCTAGG C^CTAGG BlpI 676 GCTGAGC GC^T[ACGT]AGC Bpu1102I 676 GCTGAGC GC^T[ACGT]AGC BsaBI 1353 GATGACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 484 GAGACC GGTCTC(1/5) BseAI 437 TCCGGA T^CCGGA BsiWI 149 CGTACG C^GTACG BspEI 437 TCCGGA T^CCGGA BstXI 1202 CCAGGCGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 1013 CCTCAGG CC^T[ACGT]AGG CelII 676 GCTGAGC GC^T[ACGT]AGC DraIII 367 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 1115 CCGCGG C^C[GA][CT]GG EarI 1321 CTCTTC CTCTTC(1/4) Eco81I 1013 CCTCAGG CC^T[ACGT]AGG FspI 752 TGCGCA TGC^GCA KasI 236 GGCGCC G^GCGCC Ksp632I 1321 CTCTTC CTCTTC(1/4) KspI 1115 CCGCGG CCGC^GG MamI 1353 GATGACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluI 152 ACGCGT A^CGCGT MroI 437 TCCGGA T^CCGGA NaeI 1364 GCCGGC GCC^GGC NarI 236 GGCGCC GG^CGCC NgoMIV 1364 GCCGGC G^CCGGC NotI 375 GCGGCCGC GC^GGCCGC PaeR7I 694 CTCGAG C^TCGAG PshAI 486 GACCTGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RsaI 150 GTAC GT^AC SacII 1115 CCGCGG CCGC^GG SfoI 236 GGCGCC GGC^GCC SmaI 825 CCCGGG CCC^GGG Tsp509I 1162 AATT ^AATT XcmI 1055 CCAGCTGGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 694 CTCGAG C^TCGAG XmaCI 825 CCCGGG C^CCGGG XmaI 825 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AcyI 236 GGCGCC G[GA]^CG[CT]C 745 GACGCC ApaI 144 GGGCCC GGGCC^C 1061 GGGCCC BamHI 72 GGATCC G^GATCC 1133 GGATCC BfaI 652 CTAG C^TAG 673 CTAG BsaHI 236 GGCGCC G[GA]^CG[CT]C 745 GACGCC BsaWI 75 TCCGGT [AT]^CCGG[AT] 437 TCCGGA BsmAI 484 GAGAC GTCTC(1/5) 838 GAGAC BsrFI 255 GCCGGT [GA]^CCGG[CT] 1364 GCCGGC Cfr10I 255 GCCGGT [GA]^CCGG[CT] 1364 GCCGGC EcoNI 27 CCTCCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 939 CCTACGGCAGG Hin1I 236 GGCGCC G[GA]^CG[CT]C 745 GACGCC MaeI 652 CTAG C^TAG 673 CTAG SacI 679 GAGCTC GAGCT^C 691 GAGCTC SmlI 403 CTCAAG C^T[CT][GA]AG 694 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 89 enzymes) # ######################################### AccII AciI AluI AlwI AlwNI Aor51HI AspHI AvaI AvaII BanII BbsI BbvI BcnI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EagI EclXI Eco47III Eco52I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIV PpuMI PstI PvuII Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TseI Tsp45I TspRI TthHB8I XhoII