(Circular) (Six-base) MAPSORT of: pFN21AE3963.seq Check: 8238 from: 1 to: 6109 pFN21AE3963 6109 bp With 182 enzymes: SgfI * March 28, 2012 11:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2057 2057 Size: 6109 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3919 278 Size: 53 83 186 936 2383 2468 Fragments arranged by size: 2468 2383 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3452 1937 Size: 1515 4594 Acc65I G'GTAC_C Cuts at: 3430 3430 Size: 6109 AclI AA'CG_TT Cuts at: 1563 4236 4609 1563 Size: 2673 373 3063 Fragments arranged by size: 3063 2673 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2934 4172 5556 5824 1632 Size: 1302 1238 1384 268 1917 Fragments arranged by size: 1917 1384 1302 1238 268 AfeI AGC'GCT Cuts at: 2351 2975 3011 2351 Size: 624 36 5449 Fragments arranged by size: 5449 624 36 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5912 AflIII A'CryG_T Cuts at: 1203 2157 5008 5985 1203 Size: 954 2851 977 1327 Fragments arranged by size: 2851 1327 977 954 AgeI A'CCGG_T Cuts at: 4940 4940 Size: 6109 AhdI GACnn_n'nnGTC Cuts at: 4838 4838 Size: 6109 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6109 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6109 AlwNI CAG_nnn'CTG Cuts at: 2590 2593 2602 2956 3852 5424 2590 Size: 3 9 354 896 1572 3275 Fragments arranged by size: 3275 1572 896 354 9 3 ApaI G_GGCC'C Cuts at: 2153 3070 2153 Size: 917 5192 ApaLI G'TGCA_C Cuts at: 4166 5322 4166 Size: 1156 4953 ApoI r'AATT_y Cuts at: 1493 3418 3644 3796 5905 1493 Size: 1925 226 152 2109 1697 Fragments arranged by size: 2109 1925 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4663 160 Size: 872 3631 1606 Fragments arranged by size: 3631 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6109 AvaI C'yCGr_G Cuts at: 1870 1943 2144 2165 2699 2830 3293 3307 3434 Size: 73 201 21 534 131 463 14 127 Cuts at: 3434 1870 Size: 4545 Fragments arranged by size: 4545 534 463 201 131 127 73 21 14 AvrII C'CTAG_G Cuts at: 2656 2656 Size: 6109 BamHI G'GATC_C Cuts at: 2077 3138 3439 3806 2077 Size: 1061 301 367 4380 Fragments arranged by size: 4380 1061 367 301 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2241 3430 4885 618 Size: 325 217 627 454 1189 1455 1842 Fragments arranged by size: 1842 1455 1189 627 454 325 217 BanII G_rGCy'C Cuts at: 1391 2153 2688 2700 3070 3428 1391 Size: 762 535 12 370 358 4072 Fragments arranged by size: 4072 762 535 370 358 12 BbeI G_GCGC'C Cuts at: 2245 2245 Size: 6109 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2409 2549 2922 3139 928 Size: 971 510 140 373 217 3898 Fragments arranged by size: 3898 971 510 373 217 140 BbvCI CC'TCA_GC Cuts at: 2810 2810 Size: 6109 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4300 4334 1903 Size: 34 2363 34 3678 Fragments arranged by size: 3678 2363 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4001 5211 4001 Size: 1210 4899 BclI T'GATC_A Cuts at: 1548 1548 Size: 6109 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2234 4720 136 Size: 107 122 71 631 605 562 2486 1525 Fragments arranged by size: 2486 1525 631 605 562 122 107 71 BglII A'GATC_T Cuts at: 6104 6104 Size: 6109 BlpI GC'TnA_GC Cuts at: 2682 3526 2682 Size: 844 5265 Bme1580I G_kGCm'C Cuts at: 1790 2153 2165 2282 3070 4170 5326 1790 Size: 363 12 117 788 1100 1156 2573 Fragments arranged by size: 2573 1156 1100 788 363 117 12 BmgBI CAC'GTC Cuts at: 1318 2869 1318 Size: 1551 4558 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2374 3043 3840 Size: 416 53 83 399 24 957 669 797 Cuts at: 3840 4798 442 Size: 958 1753 Fragments arranged by size: 1753 958 957 797 669 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6109 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2018 2441 2453 2624 2639 4769 1239 Size: 779 423 12 171 15 2130 2579 Fragments arranged by size: 2579 2130 779 423 171 15 12 Bpu10I CC'TnA_GC Cuts at: 2810 2898 2810 Size: 88 6021 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2393 4234 5099 5397 2393 Size: 1841 865 298 3105 Fragments arranged by size: 3105 1841 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2483 4772 882 Size: 617 984 2289 2219 Fragments arranged by size: 2289 2219 984 617 BsaAI yAC'GTr Cuts at: 493 1204 3900 493 Size: 711 2696 2702 Fragments arranged by size: 2702 2696 711 BsaBI GATnn'nnATC Cuts at: 3362 6103 3362 Size: 2741 3368 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2242 2751 3916 4298 Size: 53 83 186 936 709 509 1165 382 Cuts at: 4298 4933 275 Size: 635 1451 Fragments arranged by size: 1451 1165 936 709 635 509 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2080 2442 4542 4940 5214 5361 5772 2080 Size: 362 2100 398 274 147 411 2417 Fragments arranged by size: 2417 2100 411 398 362 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3385 3415 1178 Size: 30 165 30 1982 30 3872 Fragments arranged by size: 3872 1982 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2054 2591 2594 2621 2624 2627 1186 Size: 868 537 3 27 3 3 4668 Fragments arranged by size: 4668 868 537 27 3 3 3 BseYI C'CCAG_C Cuts at: 2133 2539 2998 3063 5312 5808 2133 Size: 406 459 65 2249 496 2434 Fragments arranged by size: 2434 2249 496 459 406 65 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2231 2842 2951 3100 1163 Size: 573 495 611 109 149 4172 Fragments arranged by size: 4172 611 573 495 149 109 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2384 2400 3374 3559 4320 5348 Size: 275 186 383 16 974 185 761 1028 Cuts at: 5348 1540 Size: 2301 Fragments arranged by size: 2301 1028 974 761 383 275 186 185 16 BsiHKAI G_wGCw'C Cuts at: 2364 2688 2700 4170 4255 5326 2364 Size: 324 12 1470 85 1071 3147 Fragments arranged by size: 3147 1470 1071 324 85 12 BsiWI C'GTAC_G Cuts at: 2154 2154 Size: 6109 BsmI GAATG_Cn' Cuts at: 3620 3713 3620 Size: 93 6016 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2153 2165 2282 2364 2688 2700 3070 Size: 399 363 12 117 82 324 12 370 Cuts at: 3070 3428 4170 4255 5326 1391 Size: 358 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 370 363 358 324 117 85 82 12 12 BspEI T'CCGG_A Cuts at: 2442 5772 2442 Size: 3330 2779 BspHI T'CATG_A Cuts at: 3998 5728 3998 Size: 1730 4379 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2057 2205 2501 3464 844 Size: 1213 148 296 963 3489 Fragments arranged by size: 3489 1213 963 296 148 BsrBI CCG'CTC Cuts at: 1394 1823 3996 1394 Size: 429 2173 3507 Fragments arranged by size: 3507 2173 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4604 4778 65 Size: 1183 3356 174 1396 Fragments arranged by size: 3356 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2260 3369 4753 4940 1700 Size: 110 450 1109 1384 187 2869 Fragments arranged by size: 2869 1384 1109 450 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6109 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6109 BssSI C'ACGA_G Cuts at: 4169 5181 4169 Size: 1012 5097 BstAPI GCAn_nnn'nTGC Cuts at: 794 2377 2536 2590 2593 2956 794 Size: 1583 159 54 3 363 3947 Fragments arranged by size: 3947 1583 363 159 54 3 BstBI TT'CG_AA Cuts at: 4913 4913 Size: 6109 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6109 BstXI CCAn_nnnn'nTGG Cuts at: 3214 3214 Size: 6109 BstYI r'GATC_y Cuts at: 1972 2077 3138 3219 3297 3439 3806 4191 4208 Size: 105 1061 81 78 142 367 385 17 Cuts at: 4208 5660 5746 5758 6104 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1061 385 367 346 142 105 86 81 78 17 12 Bsu36I CC'TnA_GG Cuts at: 3019 3019 Size: 6109 BtgI C'CryG_G Cuts at: 513 1065 2004 3120 513 Size: 552 939 1116 3502 Fragments arranged by size: 3502 1116 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2674 487 Size: 683 56 1448 3922 Fragments arranged by size: 3922 1448 683 56 BtsI GCAGTG_nn' Cuts at: 2846 3275 3625 4419 4439 2846 Size: 429 350 794 20 4516 Fragments arranged by size: 4516 794 429 350 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6109 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6074 DraI TTT'AAA Cuts at: 3413 3413 Size: 6109 DraIII CAC_nnn'GTG Cuts at: 2377 2377 Size: 6109 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5116 809 Size: 507 3800 1802 Fragments arranged by size: 3800 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2098 2226 Size: 54 1425 50 275 18 177 91 128 Cuts at: 2226 2301 2381 2397 3371 3556 4445 6074 8 Size: 75 80 16 974 185 889 1629 43 Fragments arranged by size: 1629 1425 974 889 275 185 177 128 91 80 75 54 50 43 18 16 EagI C'GGCC_G Cuts at: 1537 1812 2381 2397 3371 3556 1537 Size: 275 569 16 974 185 4090 Fragments arranged by size: 4090 974 569 275 185 16 EarI CTCTTCn'nnn_ Cuts at: 1353 3332 4039 1353 Size: 1979 707 3423 Fragments arranged by size: 3423 1979 707 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2498 2938 4696 5070 5216 387 Size: 1064 138 909 440 1758 374 146 1280 Fragments arranged by size: 1758 1280 1064 909 440 374 146 138 EcoICRI GAG'CTC Cuts at: 2686 2698 2686 Size: 12 6097 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2018 2441 2453 2624 2639 2934 4172 Size: 393 386 423 12 171 15 295 1238 Cuts at: 4172 4769 5556 5824 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1238 787 597 423 393 386 295 268 171 15 12 EcoNI CCTnn'n_nnAGG Cuts at: 2036 2948 2036 Size: 912 5197 EcoO109I rG'GnC_Cy Cuts at: 2149 2827 2941 2953 3067 3199 2149 Size: 678 114 12 114 132 5059 Fragments arranged by size: 5059 678 132 114 114 12 EcoRI G'AATT_C Cuts at: 3418 3796 3418 Size: 378 5731 FspI TGC'GCA Cuts at: 2759 4615 2759 Size: 1856 4253 HaeII r_GCGC'y Cuts at: 1678 2131 2245 2353 2673 2977 3013 5256 1678 Size: 453 114 108 320 304 36 2243 2531 Fragments arranged by size: 2531 2243 453 320 304 114 108 36 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4750 4782 4824 4856 1373 Size: 32 135 32 3178 32 42 32 2626 Fragments arranged by size: 3178 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3453 3699 669 Size: 988 281 1515 246 3079 Fragments arranged by size: 3079 1515 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6109 HpaI GTT'AAC Cuts at: 3699 3699 Size: 6109 KasI G'GCGC_C Cuts at: 2241 2241 Size: 6109 KpnI G_GTAC'C Cuts at: 3434 3434 Size: 6109 MfeI C'AATT_G Cuts at: 3708 3708 Size: 6109 MluI A'CGCG_T Cuts at: 2157 5985 2157 Size: 3828 2281 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5223 5407 1305 Size: 3918 184 2007 Fragments arranged by size: 3918 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4287 Fragments arranged by size: 4287 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4067 4426 4585 518 Size: 648 2901 359 159 2042 Fragments arranged by size: 2901 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2386 2527 2590 2709 2716 2935 3063 3122 Size: 780 141 63 119 7 219 128 59 Cuts at: 3122 3525 4202 5350 5595 1606 Size: 403 677 1148 245 2120 Fragments arranged by size: 2120 1148 780 677 403 245 219 141 128 119 63 59 7 NaeI GCC'GGC Cuts at: 3371 3371 Size: 6109 NarI GG'CG_CC Cuts at: 2242 2242 Size: 6109 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4618 Fragments arranged by size: 4618 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6109 NgoMIV G'CCGG_C Cuts at: 3369 3369 Size: 6109 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6109 NotI GC'GGCC_GC Cuts at: 2381 3556 2381 Size: 1175 4934 NspI r_CATG'y Cuts at: 1132 1229 3467 5012 1132 Size: 97 2238 1545 2229 Fragments arranged by size: 2238 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3070 1348 Size: 1722 4387 PciI A'CATG_T Cuts at: 5008 5008 Size: 6109 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6109 PfoI T'CCnGG_A Cuts at: 2034 2766 3195 2034 Size: 732 429 4948 Fragments arranged by size: 4948 732 429 PmeI GTTT'AAAC Cuts at: 3413 3413 Size: 6109 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4178 4210 5691 5723 4178 Size: 32 1481 32 4564 Fragments arranged by size: 4564 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2827 2941 3199 2827 Size: 114 258 5737 Fragments arranged by size: 5737 258 114 PshAI GACnn'nnGTC Cuts at: 1613 2495 1613 Size: 882 5227 PsiI TTA'TAA Cuts at: 3679 3679 Size: 6109 PspOMI G'GGCC_C Cuts at: 2149 3066 2149 Size: 917 5192 PspXI vC'TCGA_Gb Cuts at: 2699 2699 Size: 6109 PstI C_TGCA'G Cuts at: 830 2391 2598 2739 2784 3461 830 Size: 1561 207 141 45 677 3478 Fragments arranged by size: 3478 1561 677 207 141 45 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6109 PvuII CAG'CTG Cuts at: 2590 2716 3063 2590 Size: 126 347 5636 Fragments arranged by size: 5636 347 126 SacI G_AGCT'C Cuts at: 2688 2700 2688 Size: 12 6097 SacII CC_GC'GG Cuts at: 3123 3123 Size: 6109 SalI G'TCGA_C Cuts at: 1936 3451 1936 Size: 1515 4594 SanDI GG'GwC_CC Cuts at: 2827 3199 2827 Size: 372 5737 SbfI CC_TGCA'GG Cuts at: 3461 3461 Size: 6109 ScaI AGT'ACT Cuts at: 4357 6069 4357 Size: 1712 4397 SfcI C'TryA_G Cuts at: 826 1045 2387 2594 2735 2780 3256 3457 4592 Size: 219 1342 207 141 45 476 201 1135 Cuts at: 4592 5273 5464 826 Size: 681 191 1471 Fragments arranged by size: 1471 1342 1135 681 476 219 207 201 191 141 45 SfoI GGC'GCC Cuts at: 2243 2243 Size: 6109 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6109 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6109 SmaI CCC'GGG Cuts at: 1872 2832 3436 1872 Size: 960 604 4545 Fragments arranged by size: 4545 960 604 SmlI C'TyrA_G Cuts at: 820 1017 1943 2408 2699 4213 5114 5376 820 Size: 197 926 465 291 1514 901 262 1553 Fragments arranged by size: 1553 1514 926 901 465 291 262 197 SnaBI TAC'GTA Cuts at: 493 3900 493 Size: 3407 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6109 SphI G_CATG'C Cuts at: 1132 3467 1132 Size: 2335 3774 SspI AAT'ATT Cuts at: 5 52 4033 5 Size: 47 3981 2081 Fragments arranged by size: 3981 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6109 StyI C'CwwG_G Cuts at: 513 1065 2004 2656 2874 3183 3548 513 Size: 552 939 652 218 309 365 3074 Fragments arranged by size: 3074 939 652 552 365 309 218 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2105 2531 3220 4136 4153 4306 4491 Size: 1255 525 426 689 916 17 153 185 Cuts at: 4491 4936 325 Size: 445 1498 Fragments arranged by size: 1498 1255 916 689 525 445 426 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4355 6067 96 Size: 274 80 33 51 1443 2378 1712 138 Fragments arranged by size: 2378 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3385 3417 6069 6101 3385 Size: 32 2652 32 3393 Fragments arranged by size: 3393 2652 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6109 XbaI T'CTAG_A Cuts at: 3445 3445 Size: 6109 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3067 3067 Size: 6109 XhoI C'TCGA_G Cuts at: 1943 2699 1943 Size: 756 5353 XmaI C'CCGG_G Cuts at: 1870 2830 3434 1870 Size: 960 604 4545 Fragments arranged by size: 4545 960 604 XmnI GAAnn'nnTTC Cuts at: 4238 4238 Size: 6109 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3917 276 Size: 53 83 186 936 2383 2468 Fragments arranged by size: 2468 2383 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BaeI BfrBI BplI BsmBI BstZ17I EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PsrI RsrII SapI SexAI SfiI SrfI SwaI