(Circular) (Six-base) MAPSORT of: pFN21AE3957.seq Check: 3706 from: 1 to: 5728 pFN21AE3957 5728 bp With 182 enzymes: SgfI * March 28, 2012 11:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3538 278 Size: 53 83 186 936 2002 2468 Fragments arranged by size: 2468 2002 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3071 1937 Size: 1134 4594 Acc65I G'GTAC_C Cuts at: 3049 3049 Size: 5728 AclI AA'CG_TT Cuts at: 1563 3855 4228 1563 Size: 2292 373 3063 Fragments arranged by size: 3063 2292 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2045 2072 2240 2914 3791 5175 5443 1632 Size: 413 27 168 674 877 1384 268 1917 Fragments arranged by size: 1917 1384 877 674 413 268 168 27 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5531 AflIII A'CryG_T Cuts at: 1203 2131 4627 5604 1203 Size: 928 2496 977 1327 Fragments arranged by size: 2496 1327 977 928 AgeI A'CCGG_T Cuts at: 4559 4559 Size: 5728 AhdI GACnn_n'nnGTC Cuts at: 4457 4457 Size: 5728 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5728 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5728 AlwNI CAG_nnn'CTG Cuts at: 2068 2167 2562 3471 5043 2068 Size: 99 395 909 1572 2753 Fragments arranged by size: 2753 1572 909 395 99 ApaLI G'TGCA_C Cuts at: 3785 4941 3785 Size: 1156 4572 ApoI r'AATT_y Cuts at: 1493 3037 3263 3415 5524 1493 Size: 1544 226 152 2109 1697 Fragments arranged by size: 2109 1697 1544 226 152 AseI AT'TA_AT Cuts at: 160 1032 4282 160 Size: 872 3250 1606 Fragments arranged by size: 3250 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5728 AvaI C'yCGr_G Cuts at: 1870 1943 3053 1870 Size: 73 1110 4545 Fragments arranged by size: 4545 1110 73 BamHI G'GATC_C Cuts at: 3058 3425 3058 Size: 367 5361 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3049 4504 618 Size: 325 217 627 1262 1455 1842 Fragments arranged by size: 1842 1455 1262 627 325 217 BanII G_rGCy'C Cuts at: 1391 2202 2636 3047 1391 Size: 811 434 411 4072 Fragments arranged by size: 4072 811 434 411 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2693 928 Size: 971 794 3963 Fragments arranged by size: 3963 971 794 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3919 3953 1903 Size: 34 1982 34 3678 Fragments arranged by size: 3678 1982 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3620 4830 3620 Size: 1210 4518 BclI T'GATC_A Cuts at: 1548 1548 Size: 5728 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4339 136 Size: 107 122 71 631 605 2667 1525 Fragments arranged by size: 2667 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5723 5723 Size: 5728 BlpI GC'TnA_GC Cuts at: 3145 3145 Size: 5728 Bme1580I G_kGCm'C Cuts at: 1790 3789 4945 1790 Size: 1999 1156 2573 Fragments arranged by size: 2573 1999 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5728 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2639 2674 3459 Size: 416 53 83 399 24 1222 35 785 Cuts at: 3459 4417 442 Size: 958 1753 Fragments arranged by size: 1753 1222 958 785 416 399 83 53 35 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5728 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2060 4388 1239 Size: 821 2328 2579 Fragments arranged by size: 2579 2328 821 Bpu10I CC'TnA_GC Cuts at: 2754 2754 Size: 5728 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2599 2620 3853 4718 5016 2599 Size: 21 1233 865 298 3311 Fragments arranged by size: 3311 1233 865 298 21 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4391 882 Size: 617 2892 2219 Fragments arranged by size: 2892 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3519 493 Size: 711 2315 2702 Fragments arranged by size: 2702 2315 711 BsaBI GATnn'nnATC Cuts at: 5722 5722 Size: 5728 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3535 3917 4552 275 Size: 53 83 186 936 2002 382 635 1451 Fragments arranged by size: 2002 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4161 4559 4833 4980 5391 4161 Size: 398 274 147 411 4498 Fragments arranged by size: 4498 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2222 2252 1178 Size: 30 165 30 819 30 4654 Fragments arranged by size: 4654 819 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2024 2027 2243 1186 Size: 838 3 216 4671 Fragments arranged by size: 4671 838 216 3 BseYI C'CCAG_C Cuts at: 4931 5427 4931 Size: 496 5232 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2153 1163 Size: 573 417 4738 Fragments arranged by size: 4738 573 417 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3178 3939 4967 1540 Size: 275 186 1177 761 1028 2301 Fragments arranged by size: 2301 1177 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2202 2541 3789 3874 4945 2202 Size: 339 1248 85 1071 2985 Fragments arranged by size: 2985 1248 1071 339 85 BsmI GAATG_Cn' Cuts at: 2295 3239 3332 2295 Size: 944 93 4691 Fragments arranged by size: 4691 944 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2202 2541 2636 3047 3789 3874 4945 Size: 399 412 339 95 411 742 85 1071 Cuts at: 4945 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 412 411 399 339 95 85 BspEI T'CCGG_A Cuts at: 5391 5391 Size: 5728 BspHI T'CATG_A Cuts at: 3617 5347 3617 Size: 1730 3998 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3083 844 Size: 2239 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3615 1394 Size: 429 1792 3507 Fragments arranged by size: 3507 1792 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2270 3005 4223 4397 65 Size: 1183 1022 735 1218 174 1396 Fragments arranged by size: 1396 1218 1183 1022 735 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4372 4559 1700 Size: 110 2562 187 2869 Fragments arranged by size: 2869 2562 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5728 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5728 BssSI C'ACGA_G Cuts at: 3788 4800 3788 Size: 1012 4716 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5728 BstBI TT'CG_AA Cuts at: 4532 4532 Size: 5728 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5728 BstYI r'GATC_y Cuts at: 1972 3058 3425 3810 3827 5279 5365 5377 5723 Size: 1086 367 385 17 1452 86 12 346 Cuts at: 5723 1972 Size: 1977 Fragments arranged by size: 1977 1452 1086 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2069 2069 Size: 5728 BtgI C'CryG_G Cuts at: 513 1065 2004 2139 513 Size: 552 939 135 4102 Fragments arranged by size: 4102 939 552 135 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4989 Fragments arranged by size: 4989 683 56 BtsI GCAGTG_nn' Cuts at: 3244 4038 4058 3244 Size: 794 20 4914 Fragments arranged by size: 4914 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5728 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5693 DraI TTT'AAA Cuts at: 3032 3032 Size: 5728 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4735 809 Size: 507 3419 1802 Fragments arranged by size: 3419 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3175 4064 5693 Size: 54 1425 50 275 18 1345 889 1629 Cuts at: 5693 8 Size: 43 Fragments arranged by size: 1629 1425 1345 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3175 1537 Size: 275 1363 4090 Fragments arranged by size: 4090 1363 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2202 3658 1353 Size: 849 1456 3423 Fragments arranged by size: 3423 1456 849 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4315 4689 4835 387 Size: 1064 138 2726 374 146 1280 Fragments arranged by size: 2726 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2200 2200 Size: 5728 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2045 2060 2072 2240 2914 3791 4388 Size: 393 413 15 12 168 674 877 597 Cuts at: 4388 5175 5443 1239 Size: 787 268 1524 Fragments arranged by size: 1524 877 787 674 597 413 393 268 168 15 12 EcoNI CCTnn'n_nnAGG Cuts at: 2757 2849 2757 Size: 92 5636 EcoO109I rG'GnC_Cy Cuts at: 2438 2438 Size: 5728 EcoRI G'AATT_C Cuts at: 3037 3415 3037 Size: 378 5350 FspI TGC'GCA Cuts at: 4234 4234 Size: 5728 HaeII r_GCGC'y Cuts at: 1678 4875 1678 Size: 3197 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2706 2738 4369 4401 4443 Size: 32 135 32 1134 32 1631 32 42 Cuts at: 4443 4475 1373 Size: 32 2626 Fragments arranged by size: 2626 1631 1134 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2036 3072 3318 669 Size: 988 281 98 1036 246 3079 Fragments arranged by size: 3079 1036 988 281 246 98 HindIII A'AGCT_T Cuts at: 748 748 Size: 5728 HpaI GTT'AAC Cuts at: 3318 3318 Size: 5728 KpnI G_GTAC'C Cuts at: 3053 3053 Size: 5728 MfeI C'AATT_G Cuts at: 3327 3327 Size: 5728 MluI A'CGCG_T Cuts at: 5604 5604 Size: 5728 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2050 2683 4842 5026 1305 Size: 745 633 2159 184 2007 Fragments arranged by size: 2159 2007 745 633 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3906 Fragments arranged by size: 3906 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2942 3686 4045 4204 518 Size: 648 1776 744 359 159 2042 Fragments arranged by size: 2042 1776 744 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2739 3144 3821 4969 5214 1606 Size: 1133 405 677 1148 245 2120 Fragments arranged by size: 2120 1148 1133 677 405 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2139 513 Size: 552 939 135 4102 Fragments arranged by size: 4102 939 552 135 NdeI CA'TA_TG Cuts at: 387 387 Size: 5728 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5728 NotI GC'GGCC_GC Cuts at: 3175 3175 Size: 5728 NspI r_CATG'y Cuts at: 1132 1229 2080 2135 3086 4631 1132 Size: 97 851 55 951 1545 2229 Fragments arranged by size: 2229 1545 951 851 97 55 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5728 PciI A'CATG_T Cuts at: 2131 4627 2131 Size: 2496 3232 PflMI CCAn_nnn'nTGG Cuts at: 1108 2452 2562 1108 Size: 1344 110 4274 Fragments arranged by size: 4274 1344 110 PfoI T'CCnGG_A Cuts at: 2202 2202 Size: 5728 PmeI GTTT'AAAC Cuts at: 3032 3032 Size: 5728 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3797 3829 5310 5342 3797 Size: 32 1481 32 4183 Fragments arranged by size: 4183 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2438 2438 Size: 5728 PshAI GACnn'nnGTC Cuts at: 1613 2887 1613 Size: 1274 4454 PsiI TTA'TAA Cuts at: 3298 3298 Size: 5728 PstI C_TGCA'G Cuts at: 830 2127 3080 830 Size: 1297 953 3478 Fragments arranged by size: 3478 1297 953 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5728 PvuII CAG'CTG Cuts at: 2739 2739 Size: 5728 SacI G_AGCT'C Cuts at: 2202 2202 Size: 5728 SalI G'TCGA_C Cuts at: 1936 3070 1936 Size: 1134 4594 SbfI CC_TGCA'GG Cuts at: 3080 3080 Size: 5728 ScaI AGT'ACT Cuts at: 3976 5688 3976 Size: 1712 4016 SfcI C'TryA_G Cuts at: 826 1045 2123 3076 4211 4892 5083 826 Size: 219 1078 953 1135 681 191 1471 Fragments arranged by size: 1471 1135 1078 953 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5728 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5728 SmaI CCC'GGG Cuts at: 1872 3055 1872 Size: 1183 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2614 2635 3832 4733 4995 820 Size: 197 926 671 21 1197 901 262 1553 Fragments arranged by size: 1553 1197 926 901 671 262 197 21 SnaBI TAC'GTA Cuts at: 493 3519 493 Size: 3026 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5728 SphI G_CATG'C Cuts at: 1132 3086 1132 Size: 1954 3774 SspI AAT'ATT Cuts at: 5 52 3652 5 Size: 47 3600 2081 Fragments arranged by size: 3600 2081 47 StuI AGG'CCT Cuts at: 1517 2490 1517 Size: 973 4755 StyI C'CwwG_G Cuts at: 513 1065 2004 2139 2277 3167 513 Size: 552 939 135 138 890 3074 Fragments arranged by size: 3074 939 890 552 138 135 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2541 3755 3772 3925 4110 4555 325 Size: 1255 961 1214 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1214 961 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2430 2448 2626 Size: 274 80 33 51 1443 453 18 178 Cuts at: 2626 3974 5686 96 Size: 1348 1712 138 Fragments arranged by size: 1712 1443 1348 453 274 178 138 80 51 33 18 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2367 2399 5688 5720 2367 Size: 32 3289 32 2375 Fragments arranged by size: 3289 2375 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5728 XbaI T'CTAG_A Cuts at: 3064 3064 Size: 5728 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2284 2284 Size: 5728 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5728 XmaI C'CCGG_G Cuts at: 1870 3053 1870 Size: 1183 4545 XmnI GAAnn'nnTTC Cuts at: 2324 3857 2324 Size: 1533 4195 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3536 276 Size: 53 83 186 936 2002 2468 Fragments arranged by size: 2468 2002 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI