Restriction map for
pFN21AE1797
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# NO CUT ENZYMES ( 85 enzymes) #
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AatII Acc65I AccI AclI AflII
AflIII AgeI AhdI AlwNI AocI
AscI AseI AsnI Asp718I AspEI
AviII AvrII BamHI BbrPI BclI
BfrI BlnI BsaAI BsaBI BsgI
BsiWI BsmI Bsp1407I BspHI BspLU11I
BsrGI BssHII BssSI Bst1107I BstBI
BstZ17I Bsu36I CpoI DraIII DrdI
Eam1105I Eco81I FbaI FseI FspI
HgaI HindIII HpaI KpnI MaeII
MamI MfeI MluI MunI NcoI
NdeI NruI NspV PacI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI XbaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Alw44I 1615 GTGCAC G^TGCAC
Aor51HI 7 AGCGCT AGC^GCT
ApaLI 1615 GTGCAC G^TGCAC
Asp700I 2317 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 234 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 1297 GAAGAC GAAGAC(2/6)
BbvCI 2113 GCTGAGG CCTCAGC(-5/-2)
BcgI 2427 CGATGAGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 2110 CTAG C^TAG
BglII 2459 AGATCT A^GATCT
BpuAI 1297 GAAGAC GAAGAC(2/6)
BsaI 94 GAGACC GGTCTC(1/5)
BsiEI 1469 CGGCCG CG[GA][CT]^CG
BspDI 2524 ATCGAT AT^CGAT
BspMI 2260 GCAGGT ACCTGC(4/8)
BsrFI 1973 GCCGGC [GA]^CCGG[CT]
BstEII 132 GGTCACC G^GT[ACGT]ACC
BstPI 132 GGTCACC G^GT[ACGT]ACC
Cfr10I 1973 GCCGGC [GA]^CCGG[CT]
ClaI 2524 ATCGAT AT^CGAT
DraI 2371 TTTAAA TTT^AAA
EagI 1469 CGGCCG C^GGCCG
EclXI 1469 CGGCCG C^GGCCG
Eco47III 7 AGCGCT AGC^GCT
Eco52I 1469 CGGCCG C^GGCCG
EcoO65I 132 GGTCACC G^GT[ACGT]ACC
EcoRI 962 GAATTC G^AATTC
EcoRV 903 GATATC GAT^ATC
EcoT22I 1009 ATGCAT ATGCA^T
HincII 799 GTTGAC GT[CT]^[GA]AC
HindII 799 GTTGAC GT[CT]^[GA]AC
MaeI 2110 CTAG C^TAG
NaeI 1973 GCCGGC GCC^GGC
NgoMIV 1973 GCCGGC G^CCGGC
NheI 2109 GCTAGC G^CTAGC
NotI 1468 GCGGCCGC GC^GGCCGC
NsiI 1009 ATGCAT ATGCA^T
NspI 1536 GCATGT [GA]CATG^[CT]
PaeR7I 1125 CTCGAG C^TCGAG
PflFI 234 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1164 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 1222 GAGCTC GAGCT^C
SmaI 1826 CCCGGG CCC^GGG
Tth111I 234 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1164 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2213 CCAATCCAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1125 CTCGAG C^TCGAG
XmaCI 1826 CCCGGG C^CCGGG
XmaI 1826 CCCGGG C^CCGGG
XmnI 2317 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 30 enzymes) #
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AccIII 210 TCCGGA T^CCGGA
1683 TCCGGA
AcsI 962 GAATTC [GA]^AATT[CT]
2321 AAATTC
AcyI 1248 GGCGCC G[GA]^CG[CT]C
1483 GGCGCC
ApaI 1793 GGGCCC GGGCC^C
1811 GGGCCC
ApoI 962 GAATTC [GA]^AATT[CT]
2321 AAATTC
BalI 1032 TGGCCA TGG^CCA
2150 TGGCCA
BbeI 1248 GGCGCC GGCGC^C
1483 GGCGCC
BglI 192 GCCGGAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1628 GCCCTGGGGGC
BsaHI 1248 GGCGCC G[GA]^CG[CT]C
1483 GGCGCC
BseAI 210 TCCGGA T^CCGGA
1683 TCCGGA
BsmBI 1952 CGTCTC CGTCTC(1/5)
2473 GAGACG
BspEI 210 TCCGGA T^CCGGA
1683 TCCGGA
BsrDI 341 GCAATG GCAATG(2/0)
1742 GCAATG
BstAPI 1608 GCAGCGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1860 GCAGTGGATGC
BstXI 2072 CCAACACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2282 CCAGTGAGTTGG
Hin1I 1248 GGCGCC G[GA]^CG[CT]C
1483 GGCGCC
KasI 1248 GGCGCC G^GCGCC
1483 GGCGCC
KspI 64 CCGCGG CCGC^GG
1466 CCGCGG
MluNI 1032 TGGCCA TGG^CCA
2150 TGGCCA
MroI 210 TCCGGA T^CCGGA
1683 TCCGGA
MscI 1032 TGGCCA TGG^CCA
2150 TGGCCA
MseI 839 TTAA T^TAA
2372 TTAA
NarI 1248 GGCGCC GG^CGCC
1483 GGCGCC
PleI 641 GACTC GAGTC(4/5)
1939 GAGTC
PstI 1183 CTGCAG CTGCA^G
1816 CTGCAG
SacII 64 CCGCGG CCGC^GG
1466 CCGCGG
SfoI 1248 GGCGCC GGC^GCC
1483 GGCGCC
SmlI 1125 CTCGAG C^T[CT][GA]AG
1339 CTCAAG
StuI 1925 AGGCCT AGG^CCT
2096 AGGCCT
Tru9I 839 TTAA T^TAA
2372 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaI AvaII BanI BanII
BbvI BciVI BcnI BlpI BmyI
BpmI Bpu1102I BsaJI BsaWI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrBI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PpuMI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII