Restriction map for
pFN21AB4576
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# NO CUT ENZYMES ( 169 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BalI
BbeI BbrPI BciVI BcnI BfrI
BglII BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiWI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstUI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
DsaI Eam1105I Eco47III Eco81I EcoNI
EcoO109I EcoRI EcoRV EcoT14I EcoT22I
FseI FspI HgaI Hin1I HincII
HindII HinfI HpaI KasI KpnI
KspI MaeII MamI MfeI MluI
MluNI MroI MscI MseI MslI
MspA1I MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TfiI
Tru9I Tsp509I Tth111I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AlwNI 186 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 211 GTGCTC G[AT]GC[AT]^C
BamHI 479 GGATCC G^GATCC
BanI 322 GGTGCC G^G[CT][GA]CC
BbvCI 330 CCTCAGC CCTCAGC(-5/-2)
BcgI 74 GCAAGATCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 344 TGATCA T^GATCA
BfaI 451 CTAG C^TAG
BseRI 328 CTCCTC GAGGAG(10/8)
BsiEI 546 CGGCCG CG[GA][CT]^CG
BsiHKAI 211 GTGCTC G[AT]GC[AT]^C
BsmFI 471 GTCCC GGGAC(10/14)
BsrI 426 CCAGT ACTGG(1/-1)
BssSI 52 CTCGTG CACGAG(-5/-1)
BstEII 288 GGTGACC G^GT[ACGT]ACC
BstPI 288 GGTGACC G^GT[ACGT]ACC
CfoI 238 GCGC GCG^C
EagI 546 CGGCCG C^GGCCG
EarI 214 CTCTTC CTCTTC(1/4)
EclXI 546 CGGCCG C^GGCCG
Eco52I 546 CGGCCG C^GGCCG
EcoO65I 288 GGTGACC G^GT[ACGT]ACC
FbaI 344 TGATCA T^GATCA
HaeII 237 GGCGCT [GA]GCGC^[CT]
HapII 221 CCGG C^CGG
HhaI 238 GCGC GCG^C
HinP1I 238 GCGC G^CGC
HindIII 407 AAGCTT A^AGCTT
HpaII 221 CCGG C^CGG
Ksp632I 214 CTCTTC CTCTTC(1/4)
MaeI 451 CTAG C^TAG
MspI 221 CCGG C^CGG
NheI 450 GCTAGC G^CTAGC
PflMI 426 CCAGTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI 213 GCTCTTC GCTCTTC(1/4)
TaqI 387 TCGA T^CGA
TspRI 441 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 387 TCGA T^CGA
Van91I 426 CCAGTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
BanII 181 GGGCTC G[GA]GC[CT]^C
281 GAGCCC
BbsI 66 GAAGAC GAAGAC(2/6)
551 GAAGAC
BbvI 12 GCAGC GCAGC(8/12)
486 GCTGC
BpuAI 66 GAAGAC GAAGAC(2/6)
551 GAAGAC
BsiYI 81 CCTCGGCGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
426 CCAGTTCTTGG
BslI 81 CCTCGGCGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
426 CCAGTTCTTGG
BsmAI 457 GAGAC GTCTC(1/5)
202 GTCTC
BsmBI 201 CGTCTC CGTCTC(1/5)
457 GAGACG
BsrDI 225 CATTGC GCAATG(2/0)
146 GCAATG
BstF5I 306 CATCC GGATG(2/0)
99 GGATG
BstYI 77 AGATCC [GA]^GATC[CT]
479 GGATCC
EaeI 5 CGGCCA [CT]^GGCC[GA]
546 CGGCCG
FokI 306 CATCC GGATG(9/13)
99 GGATG
HphI 288 GGTGA GGTGA(8/7)
401 TCACC
MaeIII 289 GTGAC ^GT[ACGT]AC
439 GTCAC
MflI 77 AGATCC [GA]^GATC[CT]
479 GGATCC
TseI 12 GCAGC G^C[AT]GC
486 GCTGC
Tsp45I 289 GTGAC ^GT[GC]AC
439 GTCAC
XhoII 77 AGATCC [GA]^GATC[CT]
479 GGATCC
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# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AciI AluI AlwI AvaII BglI
BmyI BsaJI Bsp1286I BssKI BstNI
Cac8I Cfr13I DdeI DpnII EcoRII
FauI Fnu4HI HaeIII ItaI MboI
MboII MnlI MvaI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SfaNI